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                                       &          'Y,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRNVV1NNOnnn###---887AAAJJIRSR[[\ddcllltss||{<<<<<<<<;;;<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7848d0c6b474443bb172f7a3732a926bSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-16_05-53_17463130Seq18119137960_K1317_trnhjmadera96-WellK1317_H2_Eugenia_sp._trnhfKB_3730_POP7_BDTv3.mobSeqPop7z,+*)'&&&'('&&%&(*,..-,,,,,,,,-/134420-++-/3566531/.-./146788899:;<<;:8530-+(&$"  $).39>DKUbt0RvgG*obXNE=843455667:>BFIIIHJMS[bhmprtx}<d)E[gi`K+uGse[USTW[agnu~WD yF 7q559~9&#FEpmc<zwwwwwvvx}8r'X|xODqaUMJMUcuK4cX@pU@3+('''&#       !#" %*06:=???>=;72,%!!&.8BLV^dec`bpS=M+lmiHC@PjG2'#"#%&%$"   $).0/,+1DiF:dp[)*O)      *153+!8wx6WNRvPs\ ~R%  2]S;J/0z=     '3@MVWO>( QgRe~p@7.w!  )-/.*%1d i|&<s5  $&%1rp k17d8)+3:<:9?UE^7X9`EZHGNW_dgjmqtxz|}}~}}|{ywtqoligda^\[[[YWRNHDA@?@ABCDEEC>5)*X*mlh`<   "*01* >D )W#49     %7FRXXTLC7)'bSbZr!5HDm$         %:N]daWG6& *5?FGD<1%  "%''&#!  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"$"+% %/  *'% *)) ' ,5E4-?AE-751#&0*<9%,BA6)4&,=<18)Nb6?,>MMCT$OY5BPHODQQ@XzjRHPPPPPPPPP,GGTID<7pUF-F_@nS=jfl_xea,l2|m~kpsFvd<vPPrko_mA~i`q[aWkPP%qPPPPPPPmqPmqqPP,(pp,dpwBy2pvLM ]/N,wy=r=D?F(sOFX8N|W:&t[i:+dd{udi ]+`up#U* p,+jDpL?p#Rj rs88t4T UDpOJ;8$ YEN{;)G%QIC2od6ZuQu;gg)ZpVebOEu\L@`CdKp$9 v,pji6<]FPpppxp(sBpHu[Nzmcy L iq^9T9<d" {(==/$.)FN38.L9OX KY>jSJ5GQ/=,KB,'2^377{0/)&0>+F? |nlq."261^39I$jk2'S%$M=/@?+14N@&51RDP2KJSQ5(69(7SU$VK='H"xJ2UJwa?eg-FD,{PM)2!6@9Qh1DFJH J*>7,+:E:2g6XM!a";(53;J2"SEC0N?@`aJ}nVP%1C1-Q%g\#qn9d$,-d'8(MqPDU+H<?}2dO/2aj!Y.>|_~Lq'k\<&Me|`^`.J=mZ2KO'M4GF`O&gmKIK$TMh8)_9_4|B 1:xeTs~*$$o{?{V{ew93[#>-[|PKv.!5"T#eFInY)4T3.|S-_M0Wt%J5bwC4yr~rD^y><[0w[esp!6jK[AQxPAb[_dj`V]Kuk,qG!=U sCTACCTAACAATATGCGGTCGCGATCTACATACACGTGAATATGACACCGTTCTCCTTATATGTGCCCCCCTTAAAGGGCCAGCACCTTATGGAATCACTATCTTCTCCCTCATAAAATCTTGAGTGTGGATGAGAAATCTTAAACAAATTGAGCTCACCTAAGCATAGGAGAAACTCGACAACGCCACCCTAGGGGTTGTACTCGACAAAGAAGGCGATAAATAATAAAGCCCAACAGGCCATCCATGAGGGGAAATGAAAGGCGATTAATCAGAACTTTACAGCGGGAGTAGGAAACACAATCATAAACAAGTTCCAAAAAACGAGTACCCTATTCTTGTGGGGAGGAACGGCACCTCCTAGGCAAGAGGAGGGCACTGGGTTAAGGGGCCTAAACACCCACTCTGCGGGGGAAACGACACTGGTTAAGGGCCCTCCATTTTGCCTACAGTAACCCCCCCACTATAAACCGAGGCCCCGGGCCTTTACCTTTATTCGTGGGAGCGCCACCGCCGACAGATTCTAAAAGAACTTATGAGCATAACGTTTAACCCCCTGAACAAGGCAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-16 07:24:11 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtcASPtcB1PtbB1PtbBCTSPHBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA 'ODATA 'O4DATA 'ODATA 'OoDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~NLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS$QP1AM9r:P1RL9r6LP1WD9r?0P2AM9rHP2BA99LP2RL9rCPBAS99&PBAS99$PCON994PCON991PDMFPDMF$hPLOC9r-hPLOC9r(PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%$ISCAN3SMED SMLtSMPLSPACAaSPAC$aSPACAaSVER4.0SVER P=SVER,,Scal@Scan3TUBEH2Tmpr<UserphARphCH$phDYbigphQLcphTRphTR(This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtcASPtcB1PtbB1PtbBCTSPHBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA 'ODATA 'O4DATA 'ODATA 'OoDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab^FVoc^FWO_GATCFeat//^cGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~NLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS$QP1AM9r:P1RL9r6LP1WD9r?0P2AM9rHP2BA99LP2RL9rCPBAS99&PBAS99$PCON994PCON991PDMFPDMF$hPLOC9r-hPLOC9r(PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%$ISCAN3SMED SMLtSMPLSPACAaSPAC$aSPACAaSVER4.0SVER P=SVER,,Scal@Scan3TUBEH2Tmpr<UserphARphCH$phDYbigphQLcphTRphTRPF{ytl+hcDW4KuYGH[{O!QECN&0@]/0dwm\e+<}P?sw!iOup_3:E-v^gMs7M9{SHM6Fnf%)qk&YgDS7}_'AR;L6,8efj+l-uk#%C`]@zMxLrLei;E]yKoS]8qUvn}4|Mdna'"`ko`^ kg"#]eV&U)>`V2]<|':eqq?k>P"#(7z+d,BSbvIz=mcRFJ9'ngg61C"wY\V&Ip?Hds:MkO@JC)RVZW[Z!f]ZE!Q;eat6LK-fU'C,_,qz7h}l6t8OGz^n7aC.?bFW?E)X~6"den?,}!KPk;+s4U5hf6]X?K90lV5FaGwpBz.\_'glxU)Bk+7{lZp4[8e7?GOVcRohj4TIx*&`f5U_9%jE6;s1HX.M;76N8{q6.>`AJP:zdq3+fyW\j&1qTFU-[l'ny#RqAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtcASPtcAUDTlB1PtbB1PtbBCTSPHBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA 'ODATA 'O4DATA 'ODATA 'OoDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab^FVoc^FWO_GATCFeat//^cGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~NLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS$QP1AM9r:P1RL9r6LP1WD9r?0P2AM9rHP2BA99LP2RL9rCPBAS99&PBAS99$PCON994PCON991PDMFPDMF$hPLOC9r-hPLOC9r(PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%$ISCAN3SMED SMLtSMPLSPACAaSPAC$aSPACAaSVER4.0SVER P=SVER,,Scal@Scan3TUBEH2Tmpr<UserphARphCH$phDYbigphQLcphTRphTR