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'Y,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRNVV1NNOnnn###---887AAAJJIRSR[[\ddcllltss||{<<<<<<<<;;;<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 043e1a88d6274239bf24823b3cf232f9Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-16_05-53_17463130Seq18119137960_K1317_trnhjmadera96-WellK1317_G1_Begonia_sp._trnhfKB_3730_POP7_BDTv3.mobSeqPop7z(((''&&%%%%'),/23320.,,,,--,,,-/1356642.+)'&&'(*,-/01110-*&#! #(-39@HR^jx9Yy jL/udVKC>:86531/..0269;>@CGKOSVXZ]`ekqzBk4L[a]P9`/re]YWY\agoz2l pGo 5L\@yvyIC06 uRiH5Uk]Z]adfgimt} Ne!j?MweYSONOS\j OP<C2 ~$c }O/     "##"    #&+/49?DJPW]acceqD{J4/:p ~"( h~Q5&  $')))('(+5IlA>glMe ~O/!"$$$#   %,39;82*)6_!u6ps3m'&g4   '+,)#.U?6RHm vK5.17;:4-,=nV|Z3P '.2541,&    "$$$#"  'TH$.&]A67=DHHFFQnlcWRy7   $)+)#$H0Tpf6wK::BLTZ^begikklnquz}! 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P0Y}@Q`cmD^Go.- *EcVfkB6Nl@U \'IBENrVUcbcHEc}w%!(u\  u,FeMy~q8M& NFrv[.c2KVep$$0-cy<q 4xpY.D.$`}v9gaxifehRE=>YFW^GCRO=FFHCCFF;?54;>6?xMO(46-,.+3-)5,"!"  5%.%)*((" '#  ! !          $'  "   ! ! % ! 2 ,  # 0!0-'  ?!?#961%)FD'#&$.& /!:($!@-)+&ECCC$!CC,)(")78/](?'3=).53E=15.*>U(R26;CQ]/k*`JvNFC5/G*3O_AUMQ\f.NW.C+\ZeVZ"%H[@R8!W=5C.SCC,GCCGCCCG4GC4G4OY`pppp:pwppp%I>)8:I+Go ,qjpp`8p#@npd@=0\H/,SRR,`,TKdw- S<~Oy8>3dMd=l(LppdKeD A*,7R#"8A<8;UTjo!G<;F,Gd@( 4=:!&&48;!&B228 P=jZ0'YV4bg3K.A.)5:i;wB =XfY/{6G>7b-tdC K_FY(JZ#1%c.v7gVLt]g?/CN@Sge`5VJgpf?[qj`Jr =ORhwA+W*zY14 z3L "ATATCTAACAATATGCGGTGGTGTGCTACATATATTTACATATGACACCGTTGTCATAGTATACAACCCCCCAAGGGAGTGGAAGCACTACATCCTTTCTCGAGAGACCCCCAGGTAGAAAAGCGCTCAACGGCAAACGATAAAGCATGGATAAACATAGGAGAACCCCCTAAATACGGCGCCACCTTCCGAGGGGGAATAAGAGCCCCCTCACAAAAAGAGGAAGTCCCAAAACTCCCCGGTAGGGGGAACTAAGAAAAGAAAAAGCACCAAAGTCATTATTTCCCAATCGGGGAGGAAAGGAGAAAAAACCAACTCCCCTCTCCCCCGAGGGATAGGGTTTGCCCCCTGACCTTCCTAAACCCCTCCCCGCCAAGACCAAGGCCCAACCCCCTTAAATACAGAGAATAGAAACGCATGTGTTTTATTGTATAATGTCAGTGTTTAGTAACTAACCACCCAAACAGAGAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-16 07:24:12 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtrASPtrB1PtqB1PtqBCTSIBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA (PDATA (P4DATA (PDATA (PDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~H<LIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS%P1AM7P1RL3P1WD;TP2AMBP2BAFdP2RL?PBAS' PBAS%4PCON2PCON0DPDMFPDMF%PLOC,PLOC(PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%$SCAN3SMED SMLtSMPLSPACAeL2SPAC%SPACAeL2SVER4.0SVER ISVER,,Scal@Scan3TUBEG1Tmpr<UserphARphCH%/phDYbigphQLcphTRphTRƁThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtrASPtrB1PtqB1PtqBCTSIBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA (PDATA (P4DATA (PDATA (PDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabX FVocXFWO_GATCFeat//WGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~H<LIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS%P1AM7P1RL3P1WD;TP2AMBP2BAFdP2RL?PBAS' PBAS%4PCON2PCON0DPDMFPDMF%PLOC,PLOC(PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%$SCAN3SMED SMLtSMPLSPACAeL2SPAC%SPACAeL2SVER4.0SVER ISVER,,Scal@Scan3TUBEG1Tmpr<UserphARphCH%/phDYbigphQLcphTRphTR8Bb:({.X|L=6XO?Tve!@.%J^K'p=mjzs,t5/`3T6YJ:0/.p9;4^;5s#oC&Opau_l_e5 }37Rgx|ff[`#|D5x_7&OMb'~6/lD-b)jJJTMF4Fi%P p'Icf]4'Uw\Pcc{\A_}$A>AR5Z(yHPx%bg*p/YgnP:Y'((n:cqB2k31<(\vxUW?Et/;k$;~6>udrVbH`6@v0kc V&Ip?H^Po)Gr(K.L-[DW[Zeh&0w#j'"LAX;:bz]QyQ]bxd$XF$~8+!3@*7_6SvrsPoIXz7_lnqR9vC.Vq`A #pJE?RVuU$HTl:h~6O$@v=aiBx[FohiXN"}a8rOrm>vdAV4+ H,vE[lm6$9LiP%.csf8=%^GHF@LeP *">BZuGZ-p@vddD\4ApZTFS$oak"T'oA_AN;)oX6YMI){mnjH[F.A[s:>ZCCd+4k,Z)lO[vHds<-F3x[R2LxC{IF}oH,k#_'>XA[.VJz$*#sejp~DsK2FtSEH$*YYo%rF(5,fX!Hiwe2{sGiSVqrj/.3~V'z(Z=kT/n@6TdO{7>kOptpuze/WR/X2EtERPV]O8oe])%,8Ea-cZ,f*F8B,}sv&'Yj}4]'xP!t p7swnqR#$|Nq_MU3tbKpZa~Ur4_wf~Vu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OL