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"                                  +#)(  )'&                  "  (%65' .3E9=(+F<JF:;D:D:;9+<(;5:DOI71E GD8@5BQEGBQCKNQHF@DS;CAB3">7+"'2#)' "-      'Y,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRNVV1NNOnnn###---887AAAJJIRSR[[\ddcllltss||{<<<<<<<<;;;<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d968efae6f9840109bbc1ea51088d1c0Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-16_05-53_17463130Seq18119137960_K1317_trnhjmadera96-WellK1317_F4_Miconia_sp._trnhfKB_3730_POP7_BDTv3.mobSeqPop7z-../01233333320/-,+++,+*)(&%$$#""! !"$%''''&%$$$$#" "&,29AIS^jx .@P]gmni_P?+lZJ=3,&!"&),.////038=DJQYbn~=]zvV2 ~obYSOLKLNRX_gq{B0M+MVE-Y=vmkoy2l mGs0;2ScL?99=CHMQTX_k~,syLe`<Q>]F956:@EMWhF <_oiN![rO7'   !"!  !%,3<FNUXVQNVspM/{ RM,H_g e5 "##!!"##" )@g0}=h:     6fPTh4  %.2/%K5i4!Xx-       #LAqf.  !%$ 3vQ5vV[- (T/mUu8%*.158;>@ABCEFGHIKMORTUUROLIHGHHHFC@=:9:=BIS]fidS:%%P_@<&@TOg1=*@6    "*/.&Zv;LB    !',/.' 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"#!!&)*'!'Ly}U=;S}sG&      (KreB$ 8YxtU5        5o7@(dOE@;3*%'2GdyhgwoQ<0++/4:AGLPSTUWZ_eilljf`ZSLC;7:Id dI;89;81($,Ep"IXL+{cTMMQW_fjie]UNKJJJIE>6.'!     {AHA /AHDAL0KB.bcpKB_3730_POP7_BDTv3.mobtermTATCTCTGACTAGCTGCTGTTGAGTTCCATCTACAAATGGATAAGACTTTTTTATTAGTCTGAATTTAGACGAGTTTTTGGTTTGAAAGTAAGGGAGCAATCAAATTTGATTGCTCCCTTACTTTCATTGTTGACTTTAAAGTCTATTAAAGTATTTAATAAAATAAATAGAAAGAGTTTTCAAAAAATAAAAATAGATGAAGTAGTATACTATTACTTACGAGAAGTAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCAACAAGCAGAAAAAAGGCATGGTATTGTGAATCCACCATGCGCGAAAAGTTACTGTGTAACAATTACTACCTTGCGTTATCTCTGACTAGCTGCTGTTGAGTTCCATCTACAAATGGATAAGACTTTTTTATTAGTCTGAATTTAGACGAGTTTTTGGTTTGAAAGTAAGGGAGCAATCAAATTTGATTGCTCCCTTACTTTCATTGTTGACTTTAAAGTCTATTAAAGTATTTAATAAAATAAATAGAAAGAGTTTTCAAAAAATAAAAATAGATGAAGTAGTATACTATTACTTACGAGAAGTAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCAACAAGCAGAAAAAAGGCATGGTATTGTGAATCCACCATGCGCGAAAAGTTACTGTGTAACAATTACTACCTTGCGT $ATXlw)7IXcm{$1;IU_iv *3?KUcox)3=IS]hu)5=IV`lw*6AJUamw#0;EP[fq|  +6ALV`kv    $ / 9 D O Z e n y    * 4 > H S _ k v    " . 9 A M W a l w   ) 3 ? 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XW3$%R42`'\4_8&"295B/HBaP N K" X!YO ,$(b[QAD=#!!]B`c!$ ;:Q=)4+6N ,D OI5#WW*TM* +_0;BF2$DK1(7OG)49.$R*. :<YN3/C!Z MA^. @B: SO># JW4 7_U/QYB"27$YCI_GEb+d3F5<XI9]1R<Yb-Y!#R^4R ( M?< &2I BQSP4,2A2 cV \9 O#\;: = =* )>A .RD#<d\CH=~f!Q=t%Fz7+9[g( Gdzl`JON L+_ve_ qe/!8 vghby YB/0,MqM:(D_;*A5o*X<}]PgsJ"qr}"oHSTh'UE2W:eOhvp$\l'N8BF{GHEqk/?9?] t {i[b2!`.]EOw5w~7w%o 3;> - Xx Yj  )(gqa$/@|ue{kW^FqS@}K^A0( %3?#$:#',(Ex''h8 (         $$ $$$ $$Fi$$$$$$$$$$$$$+$ $$$$$$$$$ppp(xp0pdd/dpdj%_4#p;plLBN8eLw*nDQ+gGd%-{d)'&<XMd\Fp1s:WAd8p+HWpYd8p?pmi,Jm CBpLycc9SmnCD}$pj(w_@Nr*` 7iA&*17*%"5}K;w7332=C%')/a$;O&,4<- HL93cVY`FJ:I*QEDQG32.yImps-12W(0kZ]k6Xk9H`u\EowN[v(DunfwbHlf8{nw;$m'WJcUJd~{r4c;e]L}3ScOO_Dp BMu&81c/%MKh<`O)BU\?]W?-oz{lO 'n0T $G[cEbzDa-H&r>_&dfKvATATCTAACAATATGCGCTGGAGTGCTACATACACTTAAATAGGACACCCCAATAACTGTCATTACCCCCTTTAAAAGGCTGCGAGGGTGTGATAGCGTTCATTTAACCCCCCGAAAAAACACCCACAACCGACACCCGGTCCTATACTTGCCTAGCTCCCTTGCCTTCTCCTCTGAAACCTGGCCCCCCTCCGACAGCCGGAACTAGCGTATCCCTCCGAACATTACCTTCCGCATAAACAGACGTCTATAAGGAAATTAATTACTGACCATTCAACTAGCAAGACCCCCCACGTGCTATAGTGATTCTGCAACGAATGTGCCTCGACAATCTGTCGCGACTTCCGTCATTTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-16 07:24:26 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtASPtB1PtB1PtBCTSBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA "EDATA "E*DATA "EpDATA "EDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~RLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISP1AMb P1RLbP1WDb P2AMb,P2BAbbP2RLbhPBASbbnPBASbb PCONbbPCONbbXPDMFPDMFPLOCbPLOCbPSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEF4Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtASPtB1PtB1PtBCTSBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA "EDATA "E*DATA "EpDATA "EDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab'6FVoc'%FWO_GATCFeat//&GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~RLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISP1AMb P1RLbP1WDb P2AMb,P2BAbbP2RLbhPBASbbnPBASbb PCONbbPCONbbXPDMFPDMFPLOCbPLOCbPSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEF4Tmpr<UserphARphCHphDYbigphQLcphTRphTRV#\*x9*ofW)NK[>9`ht1_n8J{]t>fDb6b@*-D~=7?L+EK|T+1#4v*'bvxZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_?DNchv{"$U]`EzWkp$=RyS4*% `%c~]M:]A|7ZGM9V \+1w.@{Uae[&BTd.u?zcw~CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72Wekij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.EW<#G@njH[=K{_"#k6l2WeH?WhjH:(TV#:qGtV1<#Y7zVkWgw/|9vAMV^@Yo]p&qn~+lU,YTl_{1#p{XPTF}cyy3of)`|!-Vt2`\wmj773Ku\@\=5NDzU4oD7N**s$[-33.BkS7> YlGcRb[xy8J9tG>udrV=y`6@WBktK3c*6?FYT7~U+U*rR_"qaf/h7kZ5UPh_,zi8*$IB1s lXp~c]VHr`m9>|[Ls8kV_n9Nfcd]R039OQJM~V[OCy KJFU'.QHT]R06`NV%]?EZX/-k'iJ4~CubWk@X=<-8 )T;Fi$t_SF`|zo;LY&PE!am&,*oe?F[Z*`E }Z&[g;z%!SNmW.$7KO],n`!3o %>