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"                   ,/ /1+7&$'$%6$3/4/$& %,'(3 ' ($5 ,O'Y,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRNVV1NNOnnn###---887AAAJJIRSR[[\ddcllltss||{<<<<<<<<;;;<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b41ec98238de401b9e814c8af6f5d1ceSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-16_05-53_17463130Seq18119137960_K1317_trnhjmadera96-WellK1317_F10_Thalictrum_sp._trnhfKB_3730_POP7_BDTv3.mobSeqPop7z///.-,++**+,,--,+)(''()+++*))()*+-/0110/.../1345432101134568:;<<;851/-,*)'$" !%*17>EKPV]ep} 2FXiwzm]K8%pdZQHA;620..---,,,,-./14567789<AFKOQQPOQV`n5Oet}|sdN2rbUMIHIJKLNPU]gtF'GMf$n"`iYQPSZcmvC=Ok=Nwv[KCABEHKOSW\bkw*jM QOgN?867:?ENZl0i7QYO2K sR9( "$%&&%!    !!      %+18AJT]ejjfactf uu}-.qy! ^Sxc6  #$$$#"$,<V})e` i@%%.8AJPTTPJA7,"  $*/231-' (/58877;F\4|=OkZPFqB    2V J{yGY9($)3=DE@85DqWr-a~s'   ! !',/.+%)Lv"lu9-D (Fz}db/  CR5DcC &)+*('&(+/22-&!3_vgweB426;?ELU_jsxzwpf\W]t?LvuJ,~I+"'*+,,,.036883*=tSGp/J "&('")OK58 K@]O,H.=FJIFB>:6310111/,("!$&&# !&-3775/(! $(*,,,+)&"  (032.&  !')(#  )5@FB3 }&T    )7CJI='Nb%QD  (06973,% %+035540)!"C~65x; !&&# /Q> p~u./z-  "'/7@HMLB0ZyI+( -YsZ^m%=/J*sz0S}N%qyTC   "*00)-oib-G   !'(# 2xN9&uC     #)*'  !&'%     " 3t1ug4&163* #&# "@gX0 .<B=0.g")[8ZYw|f$dXj}O,Q5i7#9HI;$HpQt3*^;)Y5  '3>C>/YXvy*  F!R# -56-  -eBijEd(0CI@*8!Dz:9o-}/A '033/*$ #(+++)'&%%%%&&&&%"  $2<=4"@ FT"9~_ Jt (570%!+23,2mVc!}:8MTL7"1rdfVAS,CTs w>   %3;:. 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H<L9 N)!,3640-,.1466531///01221/-+)((((('$!      !*29=?=951.,,*)&# !$(*,+' .BU]S9 fw zwu~1;X~qc\g4OgI)p%J>Lzvw|ycOGZJ>97][wK L@c:|~n[MFFJRZ`dc^VLA5,$  ` Ay>A&HA_wA QKB.bcpKB_3730_POP7_BDTv3.mobtermAATTCTCTGACTAGCTGCTGTTGAGTTCCATCCACAAATGGCTAAGACTTAGGTCTTAGTGTATATGAGTCGTTGAAGTTGCAGGATCAACCCCCATTTCTTGTTCTGTCAAGAGGCTGGGGGATTGATCCCGCATTTTTTTTTTTTATAACGTAGAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACACTAAGCAAGAAATTCTCTGACTAGCTGCTGTTGAGTTCCATCCACAAATGGCTAAGACTTAGGTCTTAGTGTATATGAGTCGTTGAAGTTGCAGGATCAACCCCCATTTCTTGTTCTGTCAAGAGGCTGGGGGATTGATCCCGCATTTTTTTTTTTTATAACGTAGAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACACTAAGCAAGA'0A^cw 1>J^mz-;HS^kz+7BLWbp~*5AO]fr| %1>IUamy"+7CPZfr} $09DNZer~&1<HU_kv    & 3 @ J V a m y    & 2 A N'0A^cw 1>J^mz-;HS^kz+7BLWbp~*5AO]fr| %1>IUamy"+7CPZfr} $09DNZer~&1<HU_kv    & 3 @ J V a m y    & 2 A N %-  3/ ::$#.46>>>*>>*)>>>>6>>>6>3>>>>3>/>  ""-;;>>>>>;;>>>>>>>>;>>>>>>>>>>;>++3>;;>>>>>;'>>;>>>>>>>>11>>>>>11>/8>>>>2*3,3>>>>8>/3>888>>8>86>8/68>>>>3888>8))88&)8)&&/   %-  3/ ::$#.46>>>*>>*)>>>>6>>>6>3>>>>3>/>  ""-;;>>>>>;;>>>>>>>>;>>>>>>>>>>;>++3>;;>>>>>;'>>;>>>>>>>>11>>>>>11>/8>>>>2*3,3>>>>8>/3>888>>8>86>8/68>>>>3888>8))88&)8)&&/  W$ %Z: !,; 00c8$aRTT(0aROI\P<8F\R9ZY_: ,@+ZX""G^ )5UV E$S8Fc9@?N+45_%H,7d9D,<:cFEZ9I:=9TJX'X/! &0"W,?U" X>%5*aVV^JH6#R&=@'J2Y,Xb:MP L N(^]NU%A.9L&++ a^HIK ?@$-S;z&htV2`ysS5z>/C7H1c-En;EoX|bV +tGo,uhZ;[`bA:!DUZ8i+pg>$^/*|%he9S&i ia~gDc_O5a]V`mBfaw& Hw> H1C3v, (m~gelA_}h2FG[RNE)F<KO;@AI<A02625~49'8M<H('       B       0&8p\ j-awr, =B7X,@dg YNG?F EXpd9"pQqp[}Gzi3Q?05F ',C,>dd's_vX#S4wkKOVN>bBz3p)7QY|29C/100M'(8.R`t#/Q} #]lo?4C12.0h](D*"06-TV&-%#!J%#-KaB:6<VLr- ?G>O+J8S4H2P)6"DZ,+9++E+]#"9hlQL5jL5.W,"<EWn1XNJ>4[3FOR~;O_gQARJFmzCCACTCTAACAATATGTGGTGGTGAGCTACAAACACCGGACGATGACACAGTTCTAATCGCATATACCCCTTAAAGGAAGCACAATAACGGTTGATCCCTCGAGCTGTCACCCGAAAGAAACATAGACCTAGCTTCCAGCCAAAAAATAGCGCGGAGTTCCGCACGTCTCAACGTGTAGAAGGATGCTGCATAGTGACTCTACACAAGCAAGGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-16 07:24:59 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt<ASPt<B1Pt;B1Pt;BCTSBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA $IDATA $I.{DATA $IxDATA $IDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~mLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS EP1AMP1RL0P1WDP2AMP2BAP2RL:PBAS OPBAS xPCONYPCONPDMFPDMF \PLOCPLOC &PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N% =SCAN3SMED SMLtSMPLSPACMSPAC USPACMSVER4.0SVER SVER,,Scal@Scan3TUBEF10Tmpr<UserphARphCH sphDYbigphQLcphTRphTŔThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt<ASPt<B1Pt;B1Pt;BCTSBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA $IDATA $I.{DATA $IxDATA $IDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab+PFVoc+?FWO_GATCFeat..+GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~mLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS EP1AMP1RL0P1WDP2AMP2BAP2RL:PBAS OPBAS xPCONYPCONPDMFPDMF \PLOCPLOC &PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N% =SCAN3SMED SMLtSMPLSPACMSPAC USPACMSVER4.0SVER SVER,,Scal@Scan3TUBEF10Tmpr<UserphARphCH sphDYbigphQLcphTRphTRf\mci2a>qN8>o Ueh,9n5g~Ri6(*24;x$}FNVUCs-113xT]o7U"y6^i_y>]y&m"o`^ kS\KQpYp&!gL-GI&7.{Mco6m~2HBIUbe1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&p5S_3I|o6HvFHZ( g>}WqD@~TlW+b@cT]`AqhyY-N73K*}5Qf{GFt!PkQw}x?@1m_! hkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}OPDsvA\Fu*tnPl'Z9dgTiK]5bf+mb~97KA0E}'@yVj@dUj4ov.lN"@>oW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'84?73z<^*5 %6pebvM<$AJT[Mo"$+r:y`|ESRDujXTE-yh;}S$oa$N;172e,qz7|!f|bEdG[~{?26ttRHSK%}jx7Z]et|?%9'o *&AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt<ASPt<AUDT9cB1Pt;B1Pt;BCTSBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA $IDATA $I.{DATA $IxDATA $IDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab+PFVoc+?FWO_GATCFeat..+GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~mLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS EP1AMP1RL0P1WDP2AMP2BAP2RL:PBAS OPBAS xPCONYPCONPDMFPDMF \PLOCPLOC &PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N% =SCAN3SMED SMLtSMPLSPACMSPAC USPACMSVER4.0SVER SVER,,Scal@Scan3TUBEF10Tmpr<UserphARphCH sphDYbigphQLcphTRphTR