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'$          $             'Y,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRNVV1NNOnnn###---887AAAJJIRSR[[\ddcllltss||{<<<<<<<<;;;<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 375bdf3c01be497783c24d3a498789d1Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-16_05-53_17463130Seq18119137960_K1317_trnhjmadera96-WellK1317_E9_Mimosa_albida_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z~xrkfa]YUPLIGGIKMNLIEA==?EMU]djpw;\} /<EGD9)gBiWJ@953210/.,,-06<CJQW^dkqvz~+SK~  m5Rn]QJHKR]iu=|sB6B3i~R2 )@a3yoUn0tTw_OFCEJQY_eimrz-y@BRi sgkh$&ZtrRh>|ZE80,*('&$"    %+4BY~_QADRX\0     #(+.038AO_p~xkdifQXcND"{89(QlD`. #&)**)&! !'-39<>=:72-)$ '.6=CFFDA=962-(!   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VA6VG50E9EP 13@ %6<(D\ 8 3GV* %P2V\OG=d :MQ*Q 1M9+R XM\RC ?/]G$T?KA__M2! >E0& +N5-D=<L9"0&C"C_1d@FSM,M[_R870 \M+)TG40&YO<.9[Ybb8M.-XTU ?40@/PWUN80& ^[9@40#+#*TWM40a0 c0@G S5B8$WIN/=WQ4A)^OA%S\Mb(0?v %-Xl51n{$CR.j!Ed .:)Od -8@j#D3C,Z=gFHgvL$oaMm:B54v.U>Oq{!\Li,Do{E :q3!\m*VNiQc?ejJ#U,,$q:v  |j ?\xEx~Ui y1/[;'`0p3Q 1tI$7u[e}S)oowK:`*2c(9gW(jMI~_xFRo[m g&1 pwqSqv VTf{3.6"ouX<CR f\$H!}& $yQh>F)A{q~Dy<Fq^8e  )Q H/3 yBaZ*k&I;awn6Vcge}[GE;>GCHO79R[372;4>1909)+0)'8MO'0# %,$     2 (!  x         x   :    . !G>    + 30``5C``CC}I#``P``<#4>[``4Cz/vv`0?````L`8``v``Zadkk``L``````````CLp ) ;)PEP+6/@.\8O O[e(0DdMYxCt+(x.E2n@7o-6 0NE%pdR Drjg<e 9{Sm=XaN6@VpGRRTDY-@Dr( \7$1dSNpjD=wo'@AI>zN2 ED[ &B q&S /S1|qJ( Jc!@2J<XP d1,adcJ#3|"HuWTN.4 YU}y>b_d`p>_YXb%?5djG@9>J59x2T2%o$7 7<.(PNCA(:Y)VVm771*@ .D$</9+3U>_xp,>Z(4,^_10A<>.9Q,Rn*%@*5=/#A3!c?!)9Jp9  p(^c\Pm6_#=Y(lu<|aFK*i=]jS:CRuHd+[V{3C8<_Uf=~e^u%x+.{~x`Ut'NcfY(3[$Y>Dfowaa~gH" 3-N(v8U{b -r<@, y:<`jaX -s=IfYn@s&qlHI$`k-gaj(;"Wgy]DI_ <x\~'k=W,"c  o$17Z{ {U  ~ YhCCTCTCTAACAATATGCACAGGTGACTCTACATATATCAGAATATGGTATCGTCGTCATGGTACACGCCCCCCAAAGGGAGGGGCAGCTTTATTCCTTGCCTCCTGGGACCGCCAGAGCAAAGGTGGCAAAACCCCTTACCCGTATGCTGGAAAAACGGCTCCTCATACAGCTAAAGCACGATTGAGGGTATAAGTATACTAAACAATAAGGAGAGGAGAAAAGAAACGAACAAAAACCCCCAAACAAGGACCTCGAATACTGAAACCTTACCAGTCTCCCTCGATCTCAAGGAGGTGCGCCTGGTACACCCCTTGAGGGGTGAGTTTGCACCCAATATTCTATCATTTCTCAGGGGGCACGTATGCCCTCATCCTTATTGAGCTCGGGAGCGAGTACACCTGAGTTTCTCTGTGTTCTCCATATAGTAAAGACCCGCATGTGGCTACGTGTATAATGACGGTGTATAGTGTCTAAACAAAACAAAAAAGGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-16 07:25:00 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt#ASPt#B1Pt"B1Pt"BCTS^NBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA *bTDATA *bT9DATA *bTkDATA *bT/DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~\LIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS7P1AMKpP1RLGP1WDOJP2AMVP2BAZP2RLS$PBAS:PBAS8.PCONEPCONCPDMFPDMF8PLOC?PLOC<PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%7SCAN3SMED SMLtSMPLSPACA\SPAC8 SPACA\SVER4.0SVER ^CSVER,,Scal@Scan3TUBEE9Tmpr<UserphARphCH8)phDYbigphQLcphTRphTR This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt#ASPt#B1Pt"B1Pt"BCTS^NBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA *bTDATA *bT9DATA *bTkDATA *bT/DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTablFVoclFWO_GATCFeat//liGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~\LIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS7P1AMKpP1RLGP1WDOJP2AMVP2BAZP2RLS$PBAS:PBAS8.PCONEPCONCPDMFPDMF8PLOC?PLOC<PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%7SCAN3SMED SMLtSMPLSPACA\SPAC8 SPACA\SVER4.0SVER ^CSVER,,Scal@Scan3TUBEE9Tmpr<UserphARphCH8)phDYbigphQLcphTRphTRKat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?P#`(5d/vo$.w{W7;P??G+6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\f.\u,q0;L<|WI#5/(2S[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0oW~!Te4;,: TNc:v?Ggbmr\gP+lWAxmkp$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VD*p;I:/wEkfptir"8|oTq}A|1D@<7>{AXG9{$5t{cA&5\pGe46W+W cLaQu\oi$uRN=r_ylDbaxJK[Yle9Su3]NE^O{|cHT?Ou4[42g|5(T3#T]TcM#yKAc,2/jzJ^QJH:,p""#]eRWJT|eiB]%j'kXI"ggZiOHkij7!}WQ`QV-K[YJ(V]AmE$w^6h7fcT%GY?tYsYbk11&OJ-sP!b``ye2&d\wmj7`;L b9A?aeD{$maDDy*r`snHo*uhGlB^PY6C}_lnqR9]]?EZp9}:>Ju6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkSOj!Q4aK{WlVS23|jyHAj}W2yv!)QBm#oCu"AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt#ASPt#AUDTzB1Pt"B1Pt"BCTS^NBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA *bTDATA *bT9DATA *bTkDATA *bT/DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTablFVoclFWO_GATCFeat//liGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~\LIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS7P1AMKpP1RLGP1WDOJP2AMVP2BAZP2RLS$PBAS:PBAS8.PCONEPCONCPDMFPDMF8PLOC?PLOC<PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%7SCAN3SMED SMLtSMPLSPACA\SPAC8 SPACA\SVER4.0SVER ^CSVER,,Scal@Scan3TUBEE9Tmpr<UserphARphCH8)phDYbigphQLcphTRphTR