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 !   )     +  ") 36).#1/&3*('0($'     % # & $                                                 .           )!!!+1/5&+-D.*/2*49+.)+!"-"/1-9,D,) +17,'=;AM/;B=9;*34>#1 #"   'Y,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRNVV1NNOnnn###---887AAAJJIRSR[[\ddcllltss||{<<<<<<<<;;;<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 72e9d81677db4d7f9f07f615574180a3Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-16_05-53_17463130Seq18119137960_K1317_trnhjmadera96-Well#K1317_D4_Miconia_lonchophylla_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zeeedddeeffghhhgfeeeeeedb`][ZYZ[\]\[YVTSRQPPOMLKKMORTVWWWWXY[]^___`aaa`^ZVQMIGEEDCA?<:866677887778:=@CFIMRZdo{ >\xoO0yhZND;4/+)(()*,/1479;=>?@ACGKPTX[^bhs(R,H\fh_M/ ~R*yronoqtz-dR%_  Mo&y6)Eg@eIT}f%F".|aRLLQV\`cfkrUd(yi3` pYKEDHOZhzTR c^ ]<&       $'*,-...,*&! 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J U ` k w    + 5 A K U ^ f p y   !%3%)%,() -:54# **.+>/8'6>>%./8'6//>>/;>3+  ..&&-8;>;1>>;>>>;;>>>>6>>>>>>>>;;>"+++1"1;86;;;>>>;;>>>>>>>;>>>>;>6>>>>>>>>>>>>;;>>>>>>;1>>>>>>>>>>>>>>>>>>;>>>>>>;;>;;>>>>>>>>>;+>>>>;>>>>>1>>;8;3,+88>>;;38/;;8;;;,+,+$2-2222--3;8-8*8838828&,,88//8388,'83882/*'2&88 23/>2%'8/%8 '.2  ) !%3%)%,() -:54# **.+>/8'6>>%./8'6//>>/;>3+  ..&&-8;>;1>>;>>>;;>>>>6>>>>>>>>;;>"+++1"1;86;;;>>>;;>>>>>>>;>>>>;>6>>>>>>>>>>>>;;>>>>>>;1>>>>>>>>>>>>>>>>>>;>>>>>>;;>;;>>>>>>>>>;+>>>>;>>>>>1>>;8;3,+88>>;;38/;;8;;;,+,+$2-2222--3;8-8*8838828&,,88//8388,'83882/*'2&88 23/>2%'8/%8 '.2  )Xa_H;MJ%b G^K9=B%$-M-S.!_Z AX:F8RGF(ZVIa??+ \`2 -*'a/aCT@8" !.](6( ;: Q<`:3D=bOPN] @R%>3#;& H=`ZCG^4 _ !AR[448"]T=11YAB$[+D!N;'Y) (`G.C@!c*!S98;<.$%11> -_%Q0[=B.% >> 16D&BT IZ H D")7>O;(]J`&O1T+I,Q$0FM'PHLUZHY+_!$La<[UT098XSS+E< a!;B$K < 5>W1yn"HDflr(@;ch5'{D&' ]Ux l9#W3{,^io'{M\2[$$a1}bxB_ |4c#k VQrS1;}$I27rGW[}>EuawK9=],~iiW*_B`?nLixVUG=J:0_+lI8SN2Mca5x=v nV .4 H[4f?9UD.8|n|g]]Pm]J[J2.$ )3@*'?$,-0K` (/D @   ,      2){p pd1~W8XGdu,x%4d8%LmO@[ %QzkdvtP6/\7,8p5T:x2z2pW]=yQT/j!!QTeJd8 pr- 4lWBg# nMJ/Kxr<qoR -o'.C i$(_#64+6Bj,wuv^h ^E=#4?4)+>-P5(<"4UJ9y.Kbb=q +(%4#@/(%G/#?<':D90,F.04/"2O.),/,P 4!.:#T/)/A;!JeHJAO!!$F0'-3k(M<N0 /%2(3*jc&([[ZJq)Q^]EvP-r Sy?ua&bQD[/!5j>XiDGs%2Av.Lf@C7i_5c{(fmk2s~$>QM)b%TTAATCTAACAATATGCGGTGGAGTGCTACATACACGTAAATATGACACCCCAATAAGTCTGATTACCCCCTTTAAAGGGCTGCCAGGGTATGAAATCGCTATTCACCCCCTCTAGATATGGTAACCACCTTAATATGGCTCGGACCCAAACATAGTGGACGAATTGTCTATAAGGGTATGAATTATGCCCCCTAGACTGTTAACCCACGGCCCCGCCTTGAGTCTATATGAGGTAGTATACTATCCCTCACGAGAGGTGGACTCGCATATAGCGACGTGTATAAGGACAGTGTATAGTGATTCTTCAACGAGAAAGGGCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-16 07:24:28 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt$ASPt$B1Pt#B1Pt#BCTSQBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA "EDATA "E*DATA "EpDATA "EDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISP1AMA P1RLAP1WDA P2AMAP2BAAAP2RLAPBASAA6PBASAAPCONAAPCONAA{PDMFPDMFPLOCAPLOCAwPSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPL$$SPACMSPACSPACMSVER4.0SVER FSVER,,Scal@Scan3TUBED4Tmpr<UserphARphCHphDYbigphQLcphTRphTR 9This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt$ASPt$B1Pt#B1Pt#BCTSQBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA "EDATA "E*DATA "EpDATA "EDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab$FVoc$FWO_GATCFeat//$lGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISP1AMA P1RLAP1WDA P2AMAP2BAAAP2RLAPBASAA6PBASAAPCONAAPCONAA{PDMFPDMFPLOCAPLOCAwPSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPL$$SPACMSPACSPACMSVER4.0SVER FSVER,,Scal@Scan3TUBED4Tmpr<UserphARphCHphDYbigphQLcphTRphTR,*^(`P. O~JcW`@0'q qoVU-9k *c@{UaG6EG>dcnxgJB7`DK,*Tijh.iOW/D-]$/pTFV2TVyLyD,kSagv9'i?oX$^io4)X^UM]|ny!.qK|C!^g:G1|*dc0U+O}L6iyNmwP@rB ]:l=&3#Veqy{g_hD6z\[N.6| Vkyc p7xMO9j;q6TIUwch.sQ@_=!)}W}oQln:~]a:WkR,uKsH"Kg5!O$AUI/vO3G`8hC0rOHJJr9b6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(YzXr$cn?:;wWG{uHYs+/e_'UvBVvwp#_T99Wq3F:{SocJy>@kWgw/|9vAMV^@Yo]p&qn~+lU,YTl_{1#p{XPTF}cyy3of)`|!-Vt2`\wmj773Ku\@\=5NDzU4oD7N**s$[-33.BkS7> YlGcRb[xy8J9tG>udrV=y`6@TmkcDynWa9?QLwUF3TP.&"D|;f[5kr+E{@2Kmq1F#+9@E2]lDsSm}lk6t_;mc*=7s%4JYA/j+G)@-mq5FaT%ycX}OuK-fU'C,_,qz7h}l6t8OGz^n7aC.?bFW?E)X~6"den?,}!KPk;+s4U5hf6]X?K90lV5FaGwpBz.\_'glxU)Bk+7{lZp4[8e7?GOVcRohj4TIx*&`f5U_9%jE6;s1HX.M;76N8{q6.>`AJP:zdq3+fyW\j&1qTFU-J=(iI6WCryQ^+9K-?IzMHSF{,ComMOx8yT,I)_|_n?_;?oW~!^F7N? >S[jWcgEQm*Y6i47.r~+:mzv&1`Zkel{F&+?cfoQe=N^tpuzemMOMh7&NxOrz_l4~h@<}9W#()rR.X}e6bZ( ]'nya0#CFlc*{hAC?-4$&"Sf^wKB]A\9I*$6elkgahH;M[=g;^{AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt$ASPt$AUDT2B1Pt#B1Pt#BCTSQBufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA "EDATA "E*DATA "EpDATA "EDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab$FVoc$FWO_GATCFeat//$lGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISP1AMA P1RLAP1WDA P2AMAP2BAAAP2RLAPBASAA6PBASAAPCONAAPCONAA{PDMFPDMFPLOCAPLOCAwPSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPL$$SPACMSPACSPACMSVER4.0SVER FSVER,,Scal@Scan3TUBED4Tmpr<UserphARphCHphDYbigphQLcphTRphTR