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""!#                         "&     #'#(       ',$&,'700:/,5$4A<C5#=?,/"-\0?49E6,B-9F'';CD79'9-->"4 4 &   'Y,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRNVV1NNOnnn###---887AAAJJIRSR[[\ddcllltss||{<<<<<<<<;;;<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e48826888e2749eb8fedace602bbe38eSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-16_05-53_17463130Seq18119137960_K1317_trnhjmadera96-WellK1317_D2_Senna_sp._trnhfKB_3730_POP7_BDTv3.mobSeqPop7z      !%*/6@LZix $=Uj~lU;r`PB6.(#  ""###%(,048:=?AEJQZdp~7VrtW5zgYMD=:9:>CIPXakyYE p#be)QqG/*7UC(*qaar3is58;we\Z[]`diq{!` p8_)a[)eze`sBUl/1[9$       '0;GS\bc`\_ry*=d3K[b/$M[U,        #%''%" %,3:>@=600AnAy-uPT3*.6;<97Aa ]'VXSPIZkIv8     !   $*031+"!?~tdV]l0"()&"",Gy(cj[$ &.5970& ;xkL#=d3!",8CLRX\^^\YVTUX^emu}yl`\lYo9N"7\0!#%%&&$"#*1673*!BwXO{H@y`tA< Q)(PY Xb;} R    &0891 HmE%Ve `_*:CFEA;4,$   (166- =K DoH    !.:DIHC;1(! 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AuA&CAHuAKB.bcpKB_3730_POP7_BDTv3.mobtermAATGTCTGACTAGCTGCGGTTGAGGCTCCATCTATAAATGGATAAAATTGTGGTCCAAATATATATGAGTTTTTGAACGTAAAGGAGCAATATCAACTCTCTTGATATTGCTCCTTTACTTTCTTTTTTAGTAGTGTTTTTCTTCATATTCATACAATTTTTTTTATTTACTTCAACATTCTTTAACATTAGAAAAAGATAAATGATAAAAAATGATAAAGAAAGAATACAAATGAAAAATATCTGTAATTTTTTTTATATAGTATAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCCATAAAAAAAAAATGTCTGACTAGCTGCGGTTGAGGCTCCATCTATAAATGGATAAAATTGTGGTCCAAATATATATGAGTTTTTGAACGTAAAGGAGCAATATCAACTCTCTTGATATTGCTCCTTTACTTTCTTTTTTAGTAGTGTTTTTCTTCATATTCATACAATTTTTTTTATTTACTTCAACATTCTTTAACATTAGAAAAAGATAAATGATAAAAAATGATAAAGAAAGAATACAAATGAAAAATATCTGTAATTTTTTTTATATAGTATAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCCATAAAAAAAA->[au~ .9FU_ny.=DQ[gs}*6AMYhv +7ER\hs~(6@KUbnz $0<HT^jw)4@MYdo{ $0;FS`lv    ) 5 A O Z f r }  " . 9 D Q ] h t  " . : G S ^ j u    & 2 > H S ^ i u    ( 4 @ K W d o z   ,8ER[ht '4AN[j->[au~ .9FU_ny.=DQ[gs}*6AMYhv +7ER\hs~(6@KUbnz $0<HT^jw)4@MYdo{ $0;FS`lv    ) 5 A O Z f r }  " . 9 D Q ] h t  " . : G S ^ j u    & 2 > H S ^ i u    ( 4 @ K W d o z   ,8ER[ht '4AN[j " .!#*) 3:'4.26*:66:>+)>1/>/>>%>>>>8>>>1> &,-&;8;>>>>;>>>>>>;;>>>>>66>>>;381;&""%(;;6;;;>>>>>>>>;;3;>3>>>>>>>>>>>>;>>>;>>>>>>>66>>>>;>>>>>>>>;86;>>>>>>;806.8;>2>>>>>>;;;;88>;;>>>;>;;>;;;>>;>>>+;(.83+>>>66>>>>>>>>>>>>>>>>>>>>>1>>>>>1>>>;>>>>>>;;>>>;>>.>;>>>;;>>>>>;;>>>1;;>>;>>>8>+ %&2   " .!#*) 3:'4.26*:66:>+)>1/>/>>%>>>>8>>>1> &,-&;8;>>>>;>>>>>>;;>>>>>66>>>;381;&""%(;;6;;;>>>>>>>>;;3;>3>>>>>>>>>>>>;>>>;>>>>>>>66>>>>;>>>>>>>>;86;>>>>>>;806.8;>2>>>>>>;;;;88>;;>>>;>;;>;;;>>;>>>+;(.83+>>>66>>>>>>>>>>>>>>>>>>>>>1>>>>>1>>>;>>>>>>;;>>>;>>.>;>>>;;>>>>>;;>>>1;;>>;>>>8>+ %&2  "#9bZ@1WF D YQ>='@FC"CI$B=;1V bQ  32LWN A?!:<)N5 $C 7  \;?]24L@ER2 -&Y%+ X<IcP>#2 <c6Q^WO4E ,C7Uc-U9:RF@6+A_ST53 JBU)JF3(+<ST "S<`GI"^ _*_%R+3GC)'XB/N2_3^U(\/J(#R3L5B)AN?UB9@0<]!78AC:'/ME=T?Q5^G"@'WPbX ]I-7?51L V!A$X : PTJVH2KW &2KKGt R6>a9 Ey:eTG-U$\!B["rRyL;xZ2'J}97S3&sU$dE^9{[0YSG1VL2Q =3{Fdq019c/R[nby)B*`,2<} S8[O uI"E  0<8  fi}`q|4HA/foJ'?$p {]t  U^c(]:N]&[5!wlfrc{cp>EXJMGDYY_ZI=B9<7:.;27!-91:[P-?(#()0'($$" K"                   8~(Lpzpppp7Mp0fw;5uN%u 5q>dpd,8x'~PO c4j|L5QWJCx!-[v%?Q8*=]d- F#k#n74_ T+'8<?>,+%'#(+8*>" ;3TDD"?&8_PBAdF/0>, 0\#;y LAGK /8*9W@;4'/(<(G7-37' $.O)92>4G+F;]TP<PL*!M.UC<]0,0%+4$DqDM1R2D=k,u, #_9#.[)2."Q=6F$?;7@14CU#R(6J *@'#F&0$.E*BP+a5:<9@6?2.FU+22$'.*/!,PX0G;M>C<D XG=qqLK CTCCCTAACAATATGCGCTGGTGACGCTACATATATTTAAATATGTTAGTGCTCAACCTATATATGACCCCCCAAGGGAGTTGAAGCACTATCACCTCTCTAGAGATACCGCAAAAAGAAGCAAAAAAATAAGAGTGGACCAACATATAGATACACTCAACAAAATAAGCAACACCAGGCGCCATCTCCCCAGGGTTATATTTGCTCCTTTTCGTTCCGGACTCAGTACTTTTGTGGCCCACCTGTATTCCCCCGAATATATTAGGCCCCCGCCTGTCCTAACTTGAATAAGGACTGTGAATAGTGATTCTACAAACAGCGCGGGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-16 07:24:18 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt1ASPt1B1Pt0B1Pt0BCTSABufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA (IPDATA (IP5qDATA (IPDATA (IP֕DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~?LIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS'/P1AMF4P1RLF1P1WDF6P2AMF;P2BAFF>NP2RLF96PBASFF(PBASFF'bPCONFF0LPCONFF/PDMFPDMF'FPLOCF,zPLOCF)PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%''SCAN3SMED SMLtSMPLSPACMSPAC'?SPACMSVER4.0SVER ASVER,,Scal@Scan3TUBED2Tmpr<UserphARphCH']phDYbigphQLcphTRphTR?This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt1ASPt1B1Pt0B1Pt0BCTSABufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA (IPDATA (IP5qDATA (IPDATA (IP֕DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabOxFVocOgFWO_GATCFeat//O8GTyp!! 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S8UXabCDV_mi/fcGHO5QfOl>o %> NP2RLF96PBASFF(PBASFF'bPCONFF0LPCONFF/PDMFPDMF'FPLOCF,zPLOCF)PSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%''SCAN3SMED SMLtSMPLSPACMSPAC'?SPACMSVER4.0SVER ASVER,,Scal@Scan3TUBED2Tmpr<UserphARphCH']phDYbigphQLcphTRphTR