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  !  $ +!(($&'                                   #      'Y,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRNVV1NNOnnn###---887AAAJJIRSR[[\ddcllltss||{<<<<<<<<;;;<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fbe4fc5bcd90452084d04acc1a5d65ecSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-16_05-53_17463130Seq18119137960_K1317_trnhjmadera96-WellK1317_A5_Stellaria_ovata_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z%%%&'((((())*+-./0/.-,,-/24665433455420.-..//.-,+*)(''&$#"! 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Nd1q/  !%% %Vb<xk<_DH #%# #UF`q: 3|PsI;i<qM  NTWQ      LCad'  @@<A     &h<<h%  (bUo,@     )aWFO ! 5t%vz(iM+>e#  0c$FH)l7   #*) $kBT8     2b.[hRN!      !#%&(()*+,-../001111110000000000/-,*)('&&%$#!  0L^glrz}xsng_VME@=<=AIWkCx<7nMzsS+~8axL' !2BNUTJ9 i?[47ZX$e=a{~gFq+Hs8tf\VRQPQQRRRTX]cinu}-Y6m^'l2||H0X_K(em\QKHGGHIJKLMNNOOONLJGD@><;::99889:=?@AA@??@BDGJLOSVY[\]^bm\k"-K.z]KB=;;;<<<<<;:99889;>CIOU\eq!gk Gon&Kk\XZ`hosuutyMe '||%]8S:/-18AJSZ__][[cyB==u kT+           &2=EIGB<@V|0\9nJ   !!  !&*+*'#   "!!!    $*18AM[m -Pv8pN.^T%go1"OP!tfZMB7.'" $(+-......///-*&! $'('%" !$'+,,*'$"! !!!! !$&('%" !!$+4=CE?4%"Ct|S>^"         #)*# !a]+ tn&""        $%%"  &1=LZdie^Zfg N9:   xph_VLC91*$  !!  "'% YBAYk|2              $$"    !$%! N .r;!&/0'#f\o'_nY,L      &)& l|ca    !##! C{'%Z    4jlC    !$%$!   !"!   "%&&&'(*-/1345679;=@BCCB@=964357;>@@>:4/*%!").,"WEIZeu-      !! AZ_I(Z@       $^hlh.  ?!bS l2        $3:1{zPmHv' *:=5,)5Rp#+v"P`B89?EGHHHHHHGFFFGIJKLMPSWXUL?55L3#uG68AE@2%(I[1!! $(+,+)%!  &A}A' JATA#ߤKB.bcpKB_3730_POP7_BDTv3.mobtermAATCTCTGACTAGCTGCTATTGACGTTCCATCTACAAATGGATAAAGGTTCGTTTTTAGTTTAGTATATACGAGTTTTGGTTTCAAAAGTAAAGGGGCAATATTTGTTTCTTGATAGAACAAGAAATTTTGTATTGCTCCTTTACTAGCGGATTAAGCGTTTTTGTTAAGTAATTAATATTAATAAAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGAGCAGGAGTTTTTTAACTAAGTCCAACGTTTCTATAGTATTCGATTCTTTTTAACCGTCTCTTAATAAATAAACTGTTTTTTTTTTTTTTTTTGTTAGTAAAATAGGGTCCGTGTTATCTTTTTTTTTTATGTAGACTGAATACAGAGGATTTATTACTGAAGATTTCCGCAAAATTCATCCACGCCTGGGCCCCTTGCCCCCCCAAAGTGTGGGGGGGGGAAAAAAACCCCCCCCGAAGAGTTGCAATCTCTGACTAGCTGCTATTGACGTTCCATCTACAAATGGATAAAGGTTCGTTTTTAGTTTAGTATATACGAGTTTTGGTTTCAAAAGTAAAGGGGCAATATTTGTTTCTTGATAGAACAAGAAATTTTGTATTGCTCCTTTACTAGCGGATTAAGCGTTTTTGTTAAGTAATTAATATTAATAAAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGAGCAGGAGTTTTTTAACTAAGTCCAACGTTTCTATAGTATTCGATTCTTTTTAACCGTCTCTTAATAAATAAACTGTTTTTTTTTTTTTTTTTGTTAGTAAAATAGGGTCCGTGTTATCTTTTTTTTTTATGTAGACTGAATACAGAGGATTTATTACTGAAGATTTCCGCAAAATTCATCCACGCCTGGGCCCCTTGCCCCCCCAAAGTGTGGGGGGGGGAAAAAAACCCCCCCCGAAGAGTTGC'8U[my %4CKXcq!(4?JXfnz $/;DOYep~ )5AKValx #/:ER^gr~ "-7BOYep| #.9DQ[fr}  +5ALWbmx   + 5 @ L W b n z    ( 1 > I S [ e o w   ! * 4 > H R [ d o w   # / 8 F Q c m x   & / 9 D P Y a n w  ".7CO\ht~  ,7HP^hq{'3DO[epz #2>M]ly "4<GR]hr}"2=O[dn{ '2BT]glu *4?JT_it'8U[my %4CKXcq!(4?JXfnz $/;DOYep~ )5AKValx #/:ER^gr~ "-7BOYep| #.9DQ[fr}  +5ALWbmx   + 5 @ L W b n z    ( 1 > I S [ e o w   ! * 4 > H R [ d o w   # / 8 F Q c m x   & / 9 D P Y a n w  ".7CO\ht~  ,7HP^hq{'3DO[epz #2>M]ly "4<GR]hr}"2=O[dn{ '2BT]glu *4?JT_it $(. "'! #6*2 ()* +/1::: $1::)*-6:>/6>* 8&.'/>>'*>>>6>>>>>>>;;>>;;;>>;3""-+$">;>>;>>>>>1;>>>6>>;>;;>66>>>>>>;;>>>;'>>>>;;>>>>>;>>>>>>>>;>>>;>;;>>;>>>>>8*6>>;$>>>>>>;>>>>>;+>>>>>>>>>>>;1>;;>>;1>1$    "                          !               0.2>"  )+"     $(. "'! #6*2 ()* +/1::: $1::)*-6:>/6>* 8&.'/>>'*>>>6>>>>>>>;;>>;;;>>;3""-+$">;>>;>>>>>1;>>>6>>;>;;>66>>>>>>;;>>>;'>>>>;;>>>>>;>>>>>>>>;>>>;>;;>>;>>>>>8*6>>;$>>>>>>;>>>>>;+>>>>>>>>>>>;1>;;>>;1>1$    "                          !               0.2>"  )+"    3I:>U09W&O?E+"EC YdH PD6>)#X9O5Z*D.Z&N\'A6&&<]N"D?1 7=V!>! 5V7 >FL1= 3a"I;5;VObC)*X612W60/4&]IQOZIE;Da W6L @ G(E?<bJ3U>+Ea`HS!,# Z'V YB0$L[!N#,=F)dAQ>&=FK!Q T!+SK;L) "d#K`Ncc7TC2!.R,2c^ #^c'RWC>c!cc!#2cC!EHVR*Y!_2c_P2[_2_ac__2_b'& S[ A7'-T9C2$2 9JK6[F G][a WY'O:SOKTOODOW` B@%O2#OKGOZGO$ b[<ALO DR(2^62Oc(<QO+2C2G]$9OMJH"Y-7 BPN2(9>W1U" cdbI:B$99+C5T[~F+Qhmd -kAx,=P{$6T#Wi42#HRv}V2{]&3",EN;EOS7!is~&l>VmG j^Fd3XF3Mk_ f@\'d] ]5>%*| [L ,/8\ 4#&441)[/cnzCa30 pZ|+:6}Zg4z;Ev\](KvTcqbU__HC~ xH~9vxGrueexs7W^rf48 I  LY Q-$;_.D$8 o!}\.'; o? _eIhWsh8 hygyx>/c  ( `rte&]m`p`[\VNIM94.+-)C!)$?,"225(9 ,  + "   !   !!                     up >0L8I9p+a,f8%[8:E2mdp"%Gp>C*RemKtZZ[-8{p(MO QuP,oc- <BCe]ecj.X"vz3%FeduE  _W+L{  eMKsu!-,88PH${dRXn%=Q<op2cp[BwWH1}>|bT$j~7",W]dE+Sy#1F78,06rkQ(GqX,!8AT nS32T-/"+(&1URFY2?l78-5Tx2"5S>.58+HM;E'101N_zA,*)-$.'20 $&.-'-1+0/" *k&GgcZJD AO|,,%2g0%' 0w!<B2&g"} K$y(Qhwh76"Q-+Cj /,B|F`Tj  b.Bi!/Ux#1p w0Bqxk{}Q]]s<C bKf}8,SPJqB&@a`li4Di_wynzG_oP96i**0n;*-n7YQ|6b^p9CTCTCTAACAATATGCGATAGACATGCAACATACACGTGAATATGGATGCGTGCCAATTGAATTGTACCCTCTAAAAGCTGCCACCGGTGGGGCCCCGCTATAAGCACCTCCCTAGAGCACGAGTTAGAGTGTAGCGCTTCAACAATCGAATAAGGAGCGAACCTGATGTCCTAATTATAATTACCCCCCCGAATATCCCGACTTGCATAACGACTGTGTATAGTGATTATACAACAACAGGAACCAAAACATGAGGTTCGTCCATGCCGCGGTAATCCAATCCTCGGAAGCAACATCACACTACTTGGTTAATGCGGACACAAAAAACCGCGATAATGGCAGAATGGGAATGACATGGCGAGGAAAGGTGGAGACTGGGCAGCCTCCAGATCGCGCAGTTTACATTTTTTTTGAATAAACGCTGATTCGATGGCTTAATTAACCGAGTGTTTAAAAAAGGTCCCCAAAGTTGGTGCCGTGCTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-16 07:24:44 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt3ASPt3B1Pt2B1Pt2BCTS!BufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA "EDATA "E*DATA "EpDATA "EsDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~ tLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISUP1AMpP1RL P1WD8P2AMP2BAP2RLPBASlPBASPCON PCONPDMFPDMFlPLOCPLOCPPSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%MSCAN3SMED SMLtSMPLSPACMSPACeSPACMSVER4.0SVER !SVER,,Scal@Scan3TUBEA5Tmpr<UserphARphCHphDYbigphQLcphTRphTR%This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt3ASPt3B1Pt2B1Pt2BCTS!BufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA "EDATA "E*DATA "EpDATA "EsDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab0WFVoc0FFWO_GATCFeat..0GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~ tLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISUP1AMpP1RL P1WD8P2AMP2BAP2RLPBASlPBASPCON PCONPDMFPDMFlPLOCPLOCPPSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%MSCAN3SMED SMLtSMPLSPACMSPACeSPACMSVER4.0SVER !SVER,,Scal@Scan3TUBEA5Tmpr<UserphARphCHphDYbigphQLcphTRphTR&T`XDG7IG!Hgn+1Ab;*55p]?'KzK:*]Q{Y^@>=,8,XNKioh|m C]Z&|k1jK4|<0S1pPRuKojmqy=Y`gP#0beaA({]/wQ2a52rW.X}=1m)h>VO@0$^tc:!R/.Jh=V\*ML4zO5+znJO!p8Npd-(bcnP6GZ]#(lCKOMQw T"S2Mmn[Ca,k +9}WQ@UzbaI23ttrRXc|LVn!<|'kU$3g;Z6Obl% eIe5_@{&]t[XS_Q7u2&q6X+9mvH%lFGTF4kDtFErs4gj$7u\oi$uRNBrhylDbaxJK[Yle9Su3]NE^O{|cHT?Ou4[42g|5(T3#T]TcM#yKAc,2/jzJ^QJH:,p""#]eRWJT|eiB]%j'kXI"ggZiOHkij7!}WQ`QV-K[YJ(V]AmE$w^6h7fcT%GY?tYsYbk11&OJ-sP!b``ye2&d\wmj7`;L b9A?aeD{$maDDy*r`snHo*uhGlB^PY6C}_lnqR9]]?EZp9}:>Ju6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7z3bj6[/3-6)SE8pT/@MOsijd(DUfb9b%.!1lX0JP2HwmiFmmkf{cOI#XK,8n-k|cy[*oCfo;LY&0E(_y"%_oU@tC]Q#V>AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnPk=o?eUKGM5J_bi(l{~Gj`X0?888%g^^7FDg;AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt3ASPt3AUDT>jB1Pt2B1Pt2BCTS!BufT'NXCMNT UCTID0CTNM6CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA "EDATA "E*DATA "EpDATA "EsDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab0WFVoc0FFWO_GATCFeat..0GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~ tLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISUP1AMpP1RL P1WD8P2AMP2BAP2RLPBASlPBASPCON PCONPDMFPDMFlPLOCPLOCPPSZE`PTYPPPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5+RUNT RUNT RUNT Rate RunN""S/N%MSCAN3SMED SMLtSMPLSPACMSPACeSPACMSVER4.0SVER !SVER,,Scal@Scan3TUBEA5Tmpr<UserphARphCHphDYbigphQLcphTRphTR