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"("   ( $+"* 6$6 #' # 0((!"%                                 % "  (  * "34( 5$.$! $$)%# '& .&     .$&!($1HNEXidqk>-:q-&jX- %/HwZ@E?E3yKJgb2,xz[g<FHE/'&           &   "                                N",,III^^^{{{&&&999DDDXXXaavvv      **333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRROVVVNNNnnn$$#...888AAAJJJSSS\\aedlmlttt}||<<<;;;<<<;;;;;<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: acdfd44994624451aa91db14fc4ccfb0Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-02-01_20-53_16723130Seq18032137763_K1317_MX_trnh5fjmadera96-Well%K1317_E1_Ipomoea_dumosa_trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7zk| ]p|gcp g0MO4ok!zxseM/=C+:WN$p^QKMXm 9]rsbJ<IU>< % 9sK$*(-]2e7=CJvPW]d`jpvFz~Q~R|zWwEsmgaZToNIHC>t9/4u0.T-M-g.4/?0301223o44i4H32B0x.W,)&$+!DCEs~p ta)}%p2B V  1 - 'Q^baK 8 : _ _ d M C ; / !   ?Is6#<]x)Z  b 1  &w&0=HL_vUycREV;C3-)j%" D#}nCC ` cD&0I8k>{LFXe\h k2{1 Ebk1RhP1ZuzbD'  '3$eJ;3-(&+5BJI>+ *;A7" 7Tb]F(%.5<DJKB0 !292"!  *CRRD-  !'1@MQI4#' (=KPLE=867<GZr\:     0FRO?) 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Di 4 < 7pO '?OO=-qI~Ni>t    <kg1+aU& (_j3-`{F'FiU t/M+p6@5 8fu>VI0ctY]![%@u8 C@pj.J^+#=^l[9$2f7 R%# vqMMqA[!<sjI\2l?# ARQ-NQAF!@g8A%"Ab7A#MKB.bcpKB_3730_POP7_BDTv3.mobtermTATTTTTTTTCACGTTAAGATGTTTCTATAGCTGTGTTTTTTTTTCTTTTTATTTTTTCTTAACCTACCAAAACAATTACCTAAAAATTAAGGGGACTATTTTTTTTCACGTTAAGATGTTTCTATAGCTGTGTTTTTTTTTCTTTTTATTTTTTCTTAACCTACCAAAACAATTACCTAAAAATTAAGGGGAC $.:APWjx,CKTiz*16AES_gn~%3>O[p"4@Q]gp{ $.:APWjx,CKTiz*16AES_gn~%3>O[p"4@Q]gp{                      J? )$5F VP,C38EK'K=, =5cQ1JU8%^R[:c:HB0bPa/$53SS7ELDPKX6'DcXX6/ )P 4/_QS;7i.P')0e 0M 5  #)'I"  )y'sE{.y=@I)h3z+|- f 4 [ D %CZHC2c$ < E/ (sn;_uma'4D";9L $>IY1j/~W{oqZ.`K ;zPph4 u/bd\j^dDg8+x=x`F!6 uL2`.HX x75h)?!Hp":r7yFBiDujE~j )oVATCAGGAGGAAGAAGGCCCCCCCCCGGGCCCAAAACCCAACCCCCTCCCCCCCAAACCACCCCATCTATCCCCATCAACTTGTCCCCACCCCCTTTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-01 22:41:05 -05:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS5BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA /_TDATA /_vDATA /_DATA /_sDCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FWO_GATCGTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ڬLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISFP1AMaCP1RLaցP1WDaP2AMaىP2BAaaKP2RLaPBASaaPBASaayPCONaa PCONaaտPDMFTPDMF]PLOCaPLOCa;PSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND RUND RUND RUND RUNT 50RUNT (0RUNT 5RUNT ' Rate 0PRunN""JkS/N%>SCAN@SMED SMLtSMPL&&TSPACMSPACVSPACMSVER4.0SVER *SVER,,/Scal@Scan@TUBEE1Tmpr<UserphARphCHtphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS5BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA /_TDATA /_vDATA /_DATA /_sDCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FTabFVoc~FWO_GATCFeat..PGTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ڬLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISFP1AMaCP1RLaցP1WDaP2AMaىP2BAaaKP2RLaPBASaaPBASaayPCONaa PCONaaտPDMFTPDMF]PLOCaPLOCa;PSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND RUND RUND RUND RUNT 50RUNT (0RUNT 5RUNT ' Rate 0PRunN""JkS/N%>SCAN@SMED SMLtSMPL&&TSPACMSPACVSPACMSVER4.0SVER *SVER,,/Scal@Scan@TUBEE1Tmpr<UserphARphCHtphDYbigphQLcphTRphTRKat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?P#`(5^ivf0.rtW7RPGhH+6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\f.\u,f03hx'#[;c9]D&SYQ"1H+2G+kT#^`?&5hGQbS9YH|O[nYnuPQh^5Q1V?yRl<`QV-@[3>>BZuGZ-p@vulc*{<=pjHOo&eG1Vcy[*oCf`y6n?2x`agv`kt3G/3t\#9/hV6T]==6X12O[n/bsDU5hf61FA>&oPWTRI1]f)yA@r9s_|g+*F}t.("P~fI2P&N+E/Gz^n4.6t}VX*os7}Z&[Eb4;,}s@dUa0u:W^$B!X`7.ykI0o6Gzv&1fu4WyYZFU5FS7&3-OT5ro{`N%7`h?&03ghW~^(';_/C]^HLl}5.X}=$~I!|sp#at.D>["r87xFl@x4Kh4il5+||J4g-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+rapPj:,xNM,[*?BRCZcV]j?:{VxzTA=a:hVUDBW39ZmGTl{CbNOQFY1w??z+'Wzzq{H,lp^ksL}VK2]R[gF{\)lOY@ZWeoak"T'o0_s2#O|!f|bEdG~_ {JH:,bQ2a5|OjAZeXVm? >S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0SCAN@SMED SMLtSMPL&&TSPACMSPACVSPACMSVER4.0SVER *SVER,,/Scal@Scan@TUBEE1Tmpr<UserphARphCHtphDYbigphQLcphTRphTR