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"  & '# $+.%*$?!#"!')+0(*&#!$##2.!4,">%%+6289*1@@2-9::7.?J>GI56NK><PKSDIDHPFGKH_CE9C:>F.KDJ>CPJY80G;L\N(588D=C>/66;5%&)3(DA.<9'+ *0,A%( !&7.#0 /0'%,-# # " )!!& "# ";   !  %             !     !  !        &    $                                                                                                                             N",,III^^^{{{&&&999DDDXXXaavvv      **333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRROVVVNNNnnn$$#...888AAAJJJSSS\\aedlmlttt}||<<<;;;<<<;;;;;<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7f25bd21f10f4ca78ca1201e3fe9dd44Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-02-01_20-53_16723130Seq18032137763_K1317_MX_trnh5fjmadera96-Well K1317_D2_Senna_sp._trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7zw|2K^jv]3*xC` B b<a_!%t)C-04\7;m?+CCGLQV[_Nb4dQegghjlm/mwlk heb_\cXUPKFA='851.t+w(%#V!:F !"b"#$&H')*+++*('&=$#S!?6 | ^ Y / d D | L >Y i V   .X8Q&>qcE+ r)+V:8ETYL1 dM;10:K\fcVFBR{131@qA'g{sog g "+M6DUh}mn]NC:3-m($! 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"   "()*-3;@?5% &/2+   &-6<<6+!(/0'  "+.*#  6HQN@-  )44) *+# !#! #3<:.  !  #%%#    (.,$   '+& %"  *31( '10$ !:NTI3     '-) +0+ 4FJ=% -HVTD,+581$ ! ,F[c[F*   $#(+&  !'("    #"  '360"   *?IC0.@E<(/YqG "#$;IH8 (+% '+%$6>8'  -65*    &4?A9*)692# %01' +.$4GOK?4-+*'!2<=3$   #-584+  ->IJC7+$   *.)  ,/'#4;6(   ')!&6:0 %36,  *-( <\rwkQ2%:HKA.!2HY^S;    *9@;.  #6BA2#6>:+        %$ &@TXM5     3FNG4  ! 2;8()8=6' ,9@>6.),4;<3"/?C:) -=FE9'  ,;HRXZXTLA2!+;A<-   !<QYO7 )MnoQ11Mdj^C# 4HUYYYZ]_\QA1& 1e<@0UAیAJAKB.bcpKB_3730_POP7_BDTv3.mobtermTAAAGTTCAGCGTTTCCTTAGTGCATCTATTTTTCCCTTTGTTTCCATGGGGTGTGATATCTTTTTTGGTTTTGATGGTTTTTTTTTTTTTTTTTTGTTCCTTCTTGAATGGGGAGGTTTCTTGTAAAGTTCAGCGTTTCCTTAGTGCATCTATTTTTCCCTTTGTTTCCATGGGGTGTGATATCTTTTTTGGTTTTGATGGTTTTTTTTTTTTTTTTTTGTTCCTTCTTGAATGGGGAGGTTTCTTG %.FV_u $8MZiz!(2;CUhs";L]mx$.K]n~'2>Q_s$2;EKS_kw %.FV_u $8MZiz!(2;CUhs";L]mx$.K]n~'2>Q_s$2;EKS_kw                              4- B CO$TKV[S,%K' 5Y;Ica8RVBUXE;'='N9C b): a8bV: 3S6&*X7F :0[ R BWPY%F2?ZN 8_M3>%O/W'I3d7@5FIX\) EMU'n:Q @6TV >*.[Y%AhkdK p5Zx&9M|W P1POR&'? ^ X$~~#S}{ B * .Yxn:VrPY#0PX5#AK?Z7Y,#Su[$;=clXHi;XDt7#WmR!=dOpi5l8kURM:!1#om/ 2y+Usv, ,Xpd"1- O(7d);#>K E[ \ 5k(VJ~l V(YoAp3"s= `JtkqL0)M( !.wI:E6~l^osCxQ#X\e#:g j )VAGGGTGGGCCGCCCCTGGGCCCATCCTCTACCCGTATCGGTCCCTTGCCCATAAGATATGTACCCGGTCGCCCCCCTAAAGGCCCCCCCCCGCCGGTAAGTCGACCCCTAAAAAGCCCCCTCCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-01 22:41:09 -05:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1Pt~B1Pt~BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA /_TDATA /_DATA /_eDATA /_t+DCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FWO_GATCGTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~`LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM|P1RL| P1WD|P2AM|P2BA||P2RL|PBAS||ԨPBAS||,PCON||אPCON||PDMFTPDMFPLOC|PLOC|$PSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND RUND RUND RUND RUNT 50RUNT (0RUNT 5RUNT ' Rate 0PRunN""JkS/N%SCAN@SMED SMLtSMPL!!TSPACMSPAC SPACMSVER4.0SVER SVER,,/Scal@Scan@TUBED2Tmpr<UserphARphCH'phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1Pt~B1Pt~BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA /_TDATA /_DATA /_eDATA /_t+DCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FTabCFVoc2FWO_GATCFeat..GTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~`LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM|P1RL| P1WD|P2AM|P2BA||P2RL|PBAS||ԨPBAS||,PCON||אPCON||PDMFTPDMFPLOC|PLOC|$PSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND RUND RUND RUND RUNT 50RUNT (0RUNT 5RUNT ' Rate 0PRunN""JkS/N%SCAN@SMED SMLtSMPL!!TSPACMSPAC SPACMSVER4.0SVER SVER,,/Scal@Scan@TUBED2Tmpr<UserphARphCH'phDYbigphQLcphTRphTRYxr$c>'/_swW^$oVv+rrbf.f7y>i@UebYWg\I5Cd*9_krYZ=kZw4n,7]Ajci(l{~Gj`X0?888%g^^4tX {1t;#G)jp~0,W(YzXrDWy?0T4`FGN9\47.6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^nPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'zK\__KdrW*`)l@.u!n`%SzXbhe >k94uL'Jd"*+8@/S(`CW7;Kn|?I)_|_n?\h["r87xFl@x4Kh4ilCc|n@Gg-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+;Np@YS{j4zW2`&TA]BGmb,tJLV/D?*{|D$haygK >xP `v?{}I8w$YWmOyl}$rv/8NpFQlUAT/n@Z-I+TwM"f^@/(-[mMOMh7&05OPh~wA[h@tFt(#jyO~6X~3"F*+#dgU[_5:W^$OufcGHO5QfOl>o ZbCd]'GE#!#hmmfc4.=+I}S$o:/bEmKB6KP8)s5AVihx_7&OMb=KuRZ+kM7yW@"SD=y!.qK|-o*u0#|UUQ1V? MlYXjlnK[Yl`A #pJE,#VuU4zr)v43An\/V4{nfnkbW+7Z>0N}5!){mnjH[v]Eb,8HW>ycPJ~FT,{1:Kn6?_s2n`457^^sIdwz@{&]t[X'(A(f'PJA}*4d#kE_GY@ZWeoPTF}cyR6A;{IF}o-jAS_7ea_';_/Ft(#jvlj,DmZnyI;!H|x]0oPGlOG!>RE.$2<2oYWA8aM|@:}L0BG^O9#:7/GQY]eL4z&5OyXJd$P+Yy;3i-jE(_y"LAX;:,bQ2a5qO Jm}Pz%I!|sp:W+DO]h^5Q1V?ORJedjY{mwYBT3 #pJEK^;S4fT{-*<^kY{zsyN}@*ohj4TIx*&`_M(_yb;E2Yh^n@2dP1jpJ6~=D:W}&O[n/bsDkFhwD~EG7&o.9OQJMrVq}d7rLyEq28B]C\]A@r9I$sY;Fi$t_SF`|zo;LY&05OPh~wA[h@t?"\=QtAcfu?*M~~+N@c'v+gK >I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jL@A[]".6,x^.9M2"ZwclQ[vL}W$620eVSQ/AAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTVB1Pt~B1Pt~BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA /_TDATA /_DATA /_eDATA /_t+DCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FTabCFVoc2FWO_GATCFeat..GTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~`LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM|P1RL| P1WD|P2AM|P2BA||P2RL|PBAS||ԨPBAS||,PCON||אPCON||PDMFTPDMFPLOC|PLOC|$PSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND RUND RUND RUND RUNT 50RUNT (0RUNT 5RUNT ' Rate 0PRunN""JkS/N%SCAN@SMED SMLtSMPL!!TSPACMSPAC SPACMSVER4.0SVER SVER,,/Scal@Scan@TUBED2Tmpr<UserphARphCH'phDYbigphQLcphTRphTR