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' # %   " ! #  %                                                                                         F      N",,III^^^{{{&&&999DDDXXXaavvv      **333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRROVVVNNNnnn$$#...888AAAJJJSSS\\aedlmlttt}||<<<;;;<<<;;;;;<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bf199bf1fddc44dc9b84e4746f2d6766Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-02-01_20-53_16723130Seq18032137763_K1317_MX_trnh5fjmadera96-Well!K1317_A2_Asteraceae_trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7z|3 #t ) 9 - K d:Ufh !#(%*/7>FLRY`jw~{zs{H~/wng?ap]YWTSRS(SUV]WjWWVTQNBJHFA<72G,(V$""!! 7 qFX!&tk8'E; u"##$&(++.c1p35@667[8;>5BF?JNvQT%UW@YL]cnA}Z{ snjEhBgggfeGbK^!Y;T2OKfGDF@=;-9:L<>e@B BBCEHLQ TVXRYYZ\ ]^^_^^\BYoUQ|MIECPAA@AA@?><:"88V9:7= g Y  3  f h V:%70iQA%]GeFEo\p1\<M' '.   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(EJ!YT :c}z[, 0VdS+a[!$$'OVo'lI *31&  9 $* #11# F\"!G_]C(Lfm^@Cmm:1PpQ?*497- 1DMJ?1"V6r %FgK)%}DH0n\1 -MO2 ?sv}z 9GDG\yZ4,M}lH"2'>%~[h wX>{"K7zq)Q~\G:2Yz5wNlV8T1SX{89-E 0/wXOu>PGnda@_/D'h\Q=!2IXaeilkcR9LX0<   %# +QoymS8"E0{+ 9[sEt)+ 7XcP&(Sm>h#UvzeB +[yH  0^k>Adzya<   0xA !"mJf CCI JO)('  'tu$Fg.Nc$  "h>59 Wl' *;C?/0?D=,0@@."]Q P*ig)a/Yz}Y)]L#1(  A5?un?8kI 6/l`xDS_HBU3W?2KVbkN5>Go~X WjkK"p,"YrCM;#K>oO|~  83HGkTV} hk*}ttqkk!H?V)V7+&U6U3 S_L4KS>1 ^YaQDGkQic6>fHWa$U:]_T3P)l $& %EbuwhK( HS ;E.#dH% (+% aE:";&lg:>B Tg-obRj,21-&/CMI9$8SdlyU% %# 4qH.KUP ,$ k^Aj!$zw1tajIK\kTi@V&JL1u( .GO 39NbAK ,|'TU-m'XFR`}Um6/: ;SjJJhiKC"p-yVvjG7m:9 |(P:+`)W+ $n"F)/!cDv/ X!^TN fM %qFE-3y<mToLv za]9ZWd_  %T|`K60L u!<@A&&7. gl (0&+KntK%*MiqbE&!DzV6zP k4- rE]'tt"*YCw8W#RSG_i*QeOB&#+77*  .}=xHKRU#-DK@' 1n[ zs W%OypT5&gf:),1*,s6:#o/FWR5I{eG0!QX7$e`Z/I.U 'RP< Q{NgI= M_'c2~TB@ AAQAlKB.bcpKB_3730_POP7_BDTv3.mobtermTTAAAGTATTATATTCCTTATCTTGTGATTATTTTTTTCCTTGGGTTTTTGGGGGAACTGGAATTTACCCAGTCTGATAGGGTATTAAAGTATTATATTCCTTATCTTGTGATTATTTTTTTCCTTGGGTTTTTGGGGGAACTGGAATTTACCCAGTCTGATAGGGTA *<JShy8K`m{$)0;BPZco~#6?MWbmx #5DQ^j| *<JShy8K`m{$)0;BPZco~#6?MWbmx #5DQ^j|                29 *GZMA`Q/U*`+QDc>@T4*W#P+FVLH_= .BMFQ"]/ TLS*2CP*<*E@<?Ym\^v 8     g z]{, GPHBA# -8RRj Oc 24IUh"QuT5KjVg}s Xb HZ (q `R0gy0"qV51\t'o/M#eA5=1\ Gf~5p{kMLdG.zD[ 3;g51H 5 _g>mH"]/uv2V<7}]T[sW6NJRAAGGGAGGGCCCCCCTTGGGGGCCACCCAATGCCCCCCTGCCTTTGGCGGACCCCGCGCCCGGCCGCGGGCCGTGAGCCCCCCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-01 22:41:15 -05:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA /_TDATA /_DATA /_bDATA /_t&DCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FWO_GATCGTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMTmP1RLTP1WDTP2AMTeP2BATT P2RLTؽPBASTTyPBASTT%PCONTTqPCONTTPDMFTPDMF PLOCTuPLOCTPSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND RUND RUND RUND RUNT 50RUNT (0RUNT 5RUNT ' Rate 0PRunN""JkS/N%SCAN@SMED SMLtSMPL""TSPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEA2Tmpr<UserphARphCH phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA /_TDATA /_DATA /_bDATA /_t&DCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FTabDFVoc3FWO_GATCFeat..GTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMTmP1RLTP1WDTP2AMTeP2BATT P2RLTؽPBASTTyPBASTT%PCONTTqPCONTTPDMFTPDMF PLOCTuPLOCTPSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND RUND RUND RUND RUNT 50RUNT (0RUNT 5RUNT ' Rate 0PRunN""JkS/N%SCAN@SMED SMLtSMPL""TSPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEA2Tmpr<UserphARphCH phDYbigphQLcphTRphTRXPvc.[.Iu2Zv&1x5roz;P= =&e=1&!rE_g4Y@D}4r(>z1{zK90plkm$48v3}k76gg`q:QS#tyYZ(WxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]3Fo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEey069>R/Ury&EHtVeXciW 45=)ySQbir8r|:7'I3#wor({Y]?\/t]66{qT[sEAhiW?WUBs*u $O6%nOp$lYTPSD`^BnBCmJ1y~I,YtR%g`+m6L#BlHz24a^jmJ!]`-;z128)TK7BKv'xPP"F77jD8z{V-AMV^@Yo]p&9'*P7C4%`i$L3u$\`.!H=membzee+n[Y<,h`Fzmj7a`<{!,8HW>y;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:#a92!ap(Z~~gln:tl|3[!]"oB/mn`kI Aq@ES-wM<,kPlXV?6pyNmdD9opluA_oW~!^F7N? >S[jWcgEQm*Y6i47.r~+:mzv&1`Zkel{F&+?cfoQe=N^tpuzemMOMh7&NxOrz_l4~h@<}9W#()rR.X}e6bZ( ]'nya0#CFlc*{hAh.iOWhbk1^wK[*oCf!`LY&'!"lC+/CWVT0U3114Mw1B21NM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? sG9J31VS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8EE.Om,NeLVWYHjD(y$9eKobs>6I(C.FbZ?`gAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTWB1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA /_TDATA /_DATA /_bDATA /_t&DCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FTabDFVoc3FWO_GATCFeat..GTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMTmP1RLTP1WDTP2AMTeP2BATT P2RLTؽPBASTTyPBASTT%PCONTTqPCONTTPDMFTPDMF PLOCTuPLOCTPSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND RUND RUND RUND RUNT 50RUNT (0RUNT 5RUNT ' Rate 0PRunN""JkS/N%SCAN@SMED SMLtSMPL""TSPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEA2Tmpr<UserphARphCH phDYbigphQLcphTRphTR