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"                                  $                      N",,III^^^{{{&&&999DDDXXXaavvv      **333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRROVVVNNNnnn$$#...888AAAJJJSSS\\aedlmlttt}||<<<;;;<<<;;;;;<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9085bf6ab9f54798a70a2db640de0cf7Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-02-01_20-53_16723130Seq18032137763_K1317_MX_trnh5fjmadera96-WellK1317_A1__trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7z?3(!&Aqda~#  &a@ 3ELUkZmX`->{K @ ' g"%)/ 5<CKS[b@i-p#sW>*  2ESXVM?-5TffR2.75(  0FV\VE/  2NdmfT;$7ThlcSIM`yiUP[ozdQL[~rehrys]>  %/1* !((!!2>?5$      /98-      ! /;?8)   !&$   #! *7:0         .]{^QYqiB&!9iB| :o*[@@5c c r f $ v"<ir|^b e59K88s3_M)a| i u])[2}7aF2$$1$^P^~\. 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'.-& /;>5%*;DB6$ &++$      1ALMB. &,'    A$h@bA.AK&A7LKB.bcpKB_3730_POP7_BDTv3.mobtermCTATTTAGTAAAGTTTTTTATTACTGTTTATTTTTTTTTCTGTGTTTTTAATGATAGGTGGAAAAGGATGAGGAAATAGGGACTTTTTTTTCTCTGTTAGAGAATGTCCACATTAAAATGGGGAAGGTTTTTTTGTGCTATTTAGTAAAGTTTTTTATTACTGTTTATTTTTTTTTCTGTGTTTTTAATGATAGGTGGAAAAGGATGAGGAAATAGGGACTTTTTTTTCTCTGTTAGAGAATGTCCACATTAAAATGGGGAAGGTTTTTTTGTG&3BU`t)1DXly %3CLYit#6BQ_iy 0?PXjp} 3=JUi '/9GNV^jr| )9E&3BU`t)1DXly %3CLYit#6BQ_iy 0?PXjp} 3=JUi '/9GNV^jr| )9E                                    !S!?U/*"0B764<S,VG)( ^1D5WL./ HQO 0SL523d#dZ`U&/%F** !`'# ]O6/N"O.+F-b>6ZB72 _+*/X 1[" @#20?ON$ EV#xU/`qD $CA)o.9yfYQ@[1{F|icoEd f 1GR w7JZDL   O 5 u Rx{BF1 YX EN',# @, zKC+ 8ii4z)$*?{5RBX|G4bh>X\Qp=8pgn8d,ikd 8cpbpg}p.21VYQe@p:L1V ![l Z3Y4V9S:PlB |h`2&J/Z@\Rh 4{!)nVvX kf'e}@2Q&)s.'f5U=$TATAAAGTGGGCCCCCCGGGGGCAAACCCTNCCCCCACCTGTGTGGGGGTTGAGGTCTGAAGTCGCTCACGTACCCCCCCCTAAAGGGGCCTCTCTCGGTGTTTAAGAACACCCCCCCAAAAAGGACCCCCCGGCCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-01 22:41:14 -05:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA /_TDATA /_DATA /_0DATA /_sDCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FWO_GATCGTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISӘP1AM%P1RLP1WD7P2AM[P2BAmP2RLIPBASTPBASPCON׊PCONPDMFTPDMFӯPLOCPLOCPSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND RUND RUND RUND RUNT 50RUNT (0RUNT 5RUNT ' Rate 0PRunN""JkS/N%ӐSCAN@SMED SMLtSMPLTSPACMSPACӨSPACMSVER4.0SVER tSVER,,/Scal@Scan@TUBEA1Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA /_TDATA /_DATA /_0DATA /_sDCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FTabFVocFWO_GATCFeat..GTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISӘP1AM%P1RLP1WD7P2AM[P2BAmP2RLIPBASTPBASPCON׊PCONPDMFTPDMFӯPLOCPLOCPSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND RUND RUND RUND RUNT 50RUNT (0RUNT 5RUNT ' Rate 0PRunN""JkS/N%ӐSCAN@SMED SMLtSMPLTSPACMSPACӨSPACMSVER4.0SVER tSVER,,/Scal@Scan@TUBEA1Tmpr<UserphARphCHphDYbigphQLcphTRphTR[4\QWuMVqrj/.$I2]DbhdJ)Y*@l./uX8?\>[wA#Q9p_`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q yfkYyG|nU,6Y\s!&&f6:jX3I.b>CCHY<6(mu y-WLa)&:\.~x|MIstAhUf0L/`ZTd02$F%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KK;x+Vx*WtdE@kZKUW59$DtVzsi0E#m1[M_5?%WE4Y8+;N}m@#=57A0Uy'KD7I 9XWXYD@xDIBGJt p7B-8'i2{js.>`T95E3&iL(g34OU,i5am9Mf:EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMK?+Nhsr5%NH!I$tU)+{Ga`o2]@Vr,,|BYD!K#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OLogXY@ZB 3O{ ~VJ(/on}sF{AR8=(V-OGC#!^MrrG]wQUfBXU/7/!WYPOu?z[(F!m,E-?){mnjH[q?Eb,8#V>AxYX]Ln?,}&8)TK7<4Zm[E,f *"c{+cWL N*hbDk0'_Cm ixPPis|K/l3672'[)VB!d+:pZ5KRCmnlcEn=6;}S$X:VDSBrRL8~f)NK1EDE?]wJUo6X?/WZ#6E]bZ&[';(Y`[2gx+C!K}Z'BNp{ e,&o.jax3o^b,:V AmE$ww*{we*V&1h.iOWhbk1^wKB_,qz74*le_M%~'pEA({]/AT^ogqlp,4lpy]AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDT~~B1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA /_TDATA /_DATA /_0DATA /_sDCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FTabFVocFWO_GATCFeat..GTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISӘP1AM%P1RLP1WD7P2AM[P2BAmP2RLIPBASTPBASPCON׊PCONPDMFTPDMFӯPLOCPLOCPSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND RUND RUND RUND RUNT 50RUNT (0RUNT 5RUNT ' Rate 0PRunN""JkS/N%ӐSCAN@SMED SMLtSMPLTSPACMSPACӨSPACMSVER4.0SVER tSVER,,/Scal@Scan@TUBEA1Tmpr<UserphARphCHphDYbigphQLcphTRphTR