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  .$$&     (!9   (+%                      $    !"#$%&9:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijkl{|}~:;<=>? !"#$%&9:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijkl{|}~:;<=>?m2<,,,III^^^{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN NNFFFcccyyy   (((00/888???FFFMMMTTT[[_aagggnmmsssyyy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e4d9f80af2a74598b5f83c5ac4feb547Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-WellK1316_G5_Hyptis_sp_trnH05KB_3730_POP7_BDTv3.mobSeqPop7z 5aYF2J n= r e  M c c X J A ? 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'|6R,:q#R5  +ft99L   / ov3(A       cF w ,   (uD7I  $z@*m      CF}mqn hwg         *3*M vr       7:ZJ               !c-C}7  &h+*l,    /v9wh,  %h/9x5    #jN !&Z"   JDt3H  AUo I!0r=/|>  HEAR#    0j&V       ,j\Yq7  =y0@,yA  <0v[m6  B@jbD_PI#   F7tzDa/"T,W_C ~E  D4x`u;   0q&w8M#   /on?V*   )`@ijCg?>f@jnOL&  3v*x}5N%  H3pw>W9Ct Iia5a?DoFklJL(  &XCG,wD"  MFs*N(  $Pj=    #S!O\HZ1 0a--l?! 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(!6-,%  (#; &J|cB4,k"e!aAf&}gLb$*xu(()C3 vHOl Xd\$<|2T 0)[?%R6[^)t~S. 0\jx/FZCJqwryX3%4WEN9Gi{GM,3dUC0dfy`kM\0;U:hS`4EQq5'AGATGCGCGCTTATGAACGCCAATTCCACGTCGAACCTGCGATTCTATAATATATAAATGATACATAATACCTCCAAGCCTATATATACACCGCGTATACTGGGGGACCGTGTACACTGGGGGTCGGTATGTCCGTAAGAGTTCATGACAGGCATATTATACGAGGGAGTCGGGACGGGCGTCCACCGTTCGGCCTCGGTCTCAAGGGGGCTTCAAACCTCAAATCCTACCGGGGGCCGGGGGGTTTAGACTGGAGACCCAGGACTTTATTGAGGTTTCTCTTTCATCTGGGTCGGGGAGGAAGTCTTCTTTATGGACGGGAAGCGCTATGTTGGTCCTCGAAAAATATCGAGAATAGTCGTACTTCTTCAATAAAATGGGGGTCTCATATTCTAGGGGTTGGGAATCAGTTTCAAGTGGAGGAAGATCCTTTAGATCCTCTATGTCTTTTTTGGAAATAAGAGCAGTATACCTGTACACCAAAAGAGAGAGGGGGGCAAACCCCGTACAACCAACACTTAAAGGAGGGAAACCCCGGGCCTGCGACCGTATAGAGGTTTCCAGAGTGTAGCTATGGCTCAGGGCCTGCGAATGTCTAAAATTGTTTCATTCGTCACGGTCTATAAATTGTACAATCTGAGGGGTCTAGAAAGAGACTTGAGTATCGTTAATAGGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:11:13 -04:00AEPt?,AEPt?,APFN WAPXV2APrN 4 APrV1APrX}}4ARTNASPFASPtASPtB1PtB1PtBCTS-BufT MCMNT 3CTIDMCTNMMCTOwMCTTL 3CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA eDATA )9DATA ?TWDATA ?TADATA ?TVDATA ?TDCHTDSamDySN 3Dye#DyeN3DyeN3DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 39EVNT 3EEVNT3QEVNT3dFWO_GATCGTyp!!3HCFGCEHCFGHCFGHCFG==InScInVtLANE#LAST~~LIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISUEOfScf %P1AMHoP1RLHjP1WDHu(P2AMHP2BAP2RLHzpPBASXPBASUxPCONgPCONePPDMFWPDMFU\PLOCH`PLOCHZPSZE`PTYPMPXLBRGNmMRMXV1RMdNERMdV1RMdXERPrNMRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 3wRunN""MS/N%U=SCAN@SMED SMLtSMPLWSPACAaXSPACUUSPACAaXSVER4.0SVER "SVER,,3 SatdfScal@Scan@TUBEG5Tmpr<UserphARphCHUsphDYbigphQLcphTRphTR'؁This is the confidence range *ABI_Limits..FVocAEPt?,AEPt?,APFN WAPXV2APrN 4 APrV1APrX}}4ARTNASPFASPtASPtB1PtB1PtBCTS-BufT MCMNT 3CTIDMCTNMMCTOwMCTTL 3CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA eDATA )9DATA ?TWDATA ?TADATA ?TVDATA ?TDCHTDSamDySN 3Dye#DyeN3DyeN3DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 39EVNT 3EEVNT3QEVNT3dFTabFVocwFWO_GATCFeat//HGTyp!!3HCFGCEHCFGHCFGHCFG==InScInVtLANE#LAST~~LIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISUEOfScf %P1AMHoP1RLHjP1WDHu(P2AMHP2BAP2RLHzpPBASXPBASUxPCONgPCONePPDMFWPDMFU\PLOCH`PLOCHZPSZE`PTYPMPXLBRGNmMRMXV1RMdNERMdV1RMdXERPrNMRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 3wRunN""MS/N%U=SCAN@SMED SMLtSMPLWSPACAaXSPACUUSPACAaXSVER4.0SVER "SVER,,3 SatdfScal@Scan@TUBEG5Tmpr<UserphARphCHUsphDYbigphQLcphTRphTRY#rNT/$4OY ~?p96UI//`JiOzJ} {&kdA[L25nWsyZKh~8JePS"Fp?(J/Hcz,(,y]18`F7IOo_3@/$9E^E:D+UZ~en?R/FIOb]rDKRm*u!?Xa./l3672_4&blIk(!Y`%]RI1]f\+!@rIwYWgUTHG&/Bc1h67A"yV+L"o&Hmd1I*w&OLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`_dEKW\lWQ7M31pfQ>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W*c3oAa!jv3}H1,Cz#Z|#e1a_WTDr]?'Hrz9Drv4~K3qoI>*?ag5d7m``k:!.tk}Y=52r =&e=.7I 9'kT'_C:j|(5,grOT1 x>|+d;5HMhk3NK;.iL(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@Cz6AqdIqN6`aD/`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT+(aQQxnJilXSXW .C=vz^`%C];p3TVuLg,r996qf}sGp$H+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3oW~!^F7N? >S[jWcgEQm*Y6i47.r~+:mzv&1`Zkel{F&+?cfoQe=N^tpuzemMOMh7&NxOrz_l4~h@<}9W#()rR.X}e6bZ( ]'nya0#CFlc*{hAC?-4$&"Sf^wKB]A\9I*$6elkgahH;M[=g;^{AEPt?,AEPt?,APFN WAPXV2APrN 4 APrV1APrX}}4ARTNASPFASPtASPtAUDT[B1PtB1PtBCTS-BufT MCMNT 3CTIDMCTNMMCTOwMCTTL 3CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA eDATA )9DATA ?TWDATA ?TADATA ?TVDATA ?TDCHTDSamDySN 3Dye#DyeN3DyeN3DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 39EVNT 3EEVNT3QEVNT3dFTabFVocwFWO_GATCFeat//HGTyp!!3HCFGCEHCFGHCFGHCFG==InScInVtLANE#LAST~~LIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISUEOfScf %P1AMHoP1RLHjP1WDHu(P2AMHP2BAP2RLHzpPBASXPBASUxPCONgPCONePPDMFWPDMFU\PLOCH`PLOCHZPSZE`PTYPMPXLBRGNmMRMXV1RMdNERMdV1RMdXERPrNMRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 3wRunN""MS/N%U=SCAN@SMED SMLtSMPLWSPACAaXSPACUUSPACAaXSVER4.0SVER "SVER,,3 SatdfScal@Scan@TUBEG5Tmpr<UserphARphCHUsphDYbigphQLcphTRphTR