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(((00/888???FFFMMMTTT[[_aagggnmmsssyyy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2f9179d5676b4e33861818157e9fe35dSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-WellK1316_F4_Pilea_sp_trnH05KB_3730_POP7_BDTv3.mobSeqPop7z7h!W9T7W  RcO!#%'@((u(y(Y(A(L(|())Z)))))))))))*5*U*))(C&f$!P|3;].) 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"""!     @eAASAKB.bcpKB_3730_POP7_BDTv3.mobtermAAAAGGTACCGCTCTAACTGATGTGAGACTTGGCTCATCCGCCCCCTCGCCTACTTCCATTCTATTTTTACATTATTTAAATTAGTTAGAAAACAAAAGACTCAAGTTCGAATATTTCTTGCTTACTTTTTCTATAATGTAAACAAAAAAGTATATGTAAGTAAAATACTAGTAAATTAATAAATAAAAAGAAAAAAAAAAGAAAGGAGCAATAGCACCCTCTTGATAGACCAAGAAAATGATTATTGCTCCTTTCTTTTCAAAACCTCCTATAGACTAAAGACTAAACTAGGATCTTATCCATTTGTAGATGGAGCTTCGATAGCAGCTAAGTCTAGAGGGAAGTTAGGAGCATTACGTTGATGCATAAACGCGCGAAAGGGCTTCTATTGCTCCTTTTCCCTTTTCACCTCCTATACAATCGGACTAATGTCTCATCTATTTTTAGTTTGAGCTTCAAAAGCAGCTGTTTCTAAAGGGAAATTATGAGCGTTACGTGTCGCTTCCAACCAGTAAAAGGTACCGCTCTAACTGATGTGAGACTTGGCTCATCCGCCCCCTCGCCTACTTCCATTCTATTTTTACATTATTTAAATTAGTTAGAAAACAAAAGACTCAAGTTCGAATATTTCTTGCTTACTTTTTCTATAATGTAAACAAAAAAGTATATGTAAGTAAAATACTAGTAAATTAATAAATAAAAAGAAAAAAAAAAGAAAGGAGCAATAGCACCCTCTTGATAGACCAAGAAAATGATTATTGCTCCTTTCTTTTCAAAACCTCCTATAGACTAAAGACTAAACTAGGATCTTATCCATTTGTAGATGGAGCTTCGATAGCAGCTAAGTCTAGAGGGAAGTTAGGAGCATTACGTTGATGCATAAACGCGCGAAAGGGCTTCTATTGCTCCTTTTCCCTTTTCACCTCCTATACAATCGGACTAATGTCTCATCTATTTTTAGTTTGAGCTTCAAAAGCAGCTGTTTCTAAAGGGAAATTATGAGCGTTACGTGTCGCTTCCAACCAGT#8COfny "-7EMY_jy %-6@OYcp{ #0<IQ[cp~ "/;FQ\fp{ #0;GQ\iu +7AMXco{)5@N[fr~%1=IT`lw    ' 2 > I U a m x    ( 5 B N [ g r    + 7 B N Y d q }  + 6 B M Y f r }  , 7 C N Z f q ~  $0<GS_jv(3@KWcoz "-8COZfr}#.<JUbnz*7@OZfr)4@JXbmy(4ALXcrz)6AKU_jy)6BN[fo{0<GPYdq}#8COfny "-7EMY_jy %-6@OYcp{ #0<IQ[cp~ "/;FQ\fp{ #0;GQ\iu +7AMXco{)5@N[fr~%1=IT`lw    ' 2 > I U a m x    ( 5 B N [ g r    + 7 B N Y d q }  + 6 B M Y f r }  , 7 C N Z f q ~  $0<GS_jv(3@KWcoz "-8COZfr}#.<JUbnz*7@OZfr)4@JXbmy(4ALXcrz)6AKU_jy)6BN[fo{0<GPYdq}   **' .,/ 8333>>> 3>;;>>;3;;;;;;;;;331#.3+.683;;;;.-;;8;;>>>>>>>>>>>88;;;;>>>>;;;>;;8>86;>>;>>>;>;>>;>66;;>8;;>1>;;;;;26>>>>>*>>>>>>>>>>>>>>>>>>>>>>;>>>>>>;>;;;>+;88>;66;;>;>>;>;;8;8>>;>;>>;;>>>>;;;;;;;32;;>>>>>;;8;;8+>;;8>;;>;;;>;;>;;8>;>>;38;;;>>>;>>>>;>>>>>>>;81110>>;>883"#(   +2"   +     *         !      ""        )          **' .,/ 8333>>> 3>;;>>;3;;;;;;;;;331#.3+.683;;;;.-;;8;;>>>>>>>>>>>88;;;;>>>>;;;>;;8>86;>>;>>>;>;>>;>66;;>8;;>1>;;;;;26>>>>>*>>>>>>>>>>>>>>>>>>>>>>;>>>>>>;>;;;>+;88>;66;;>;>>;>;;8;8>>;>;>>;;>>>>;;;;;;;32;;>>>>>;;8;;8+>;;8>;;>;;;>;;>;;8>;>>;38;;;>>>;>>>>;>>>>>>>;81110>>;>883"#(   +2"   +     *         !      ""        )       ?R Y/*5ZDGI=,\^#"/9\@ ^>LG;X5 Dac2\(<87SER+a5_d[AF_8N*(ZH*)^/bQU>J'A).c[MA-H\bM]F&*0d">N"%#SPI!%7cPS6 R.^F/" B =*M4`" 4A4AK#Y ;JKD=`ZWT1.2 ];W'K;2T4 ?O!Z[C,L@Y0P``.+TP R C7`H'@"4c(B6=PL)b: GRa$! F(aEO+<, YINC&VL,J*# S%OE6 c#)F +Z#% *N,L+D?(I:GT$_9W ab[N0' /I$>`E%%:U^Fc.BV02RD-Mc5 @R)&407FNQ1"BDQ29;P++:5ZB\W,E&V+b"8H+?'6&UHVJZbTU4X:9G=&8I_&*#_V/BG/Z6$!,%!:/%A0:`835 ;_<UKODZN5*:PN6>EGHJ a +,@ O{ rbbSF  <rD x7R"c-d/Bgl5|#j`5 uAokxrn #a0uE av]%:A DD )L|*.m 4MB%.L1qwKuG<,fSYdfBq!IEMqk+VOO2E.C?ZNsR8GFf m #j> (ux 3hR/A4kDzN <[&q_hc&a +z` O_;^qd L O_Zl-?TuawfbJY ) 2V9\~. t v 0 xi"B,?N  69%Ncz<yGXPkTH3FGm u IVf / ` ` `9!5KVrSMOZHf\mCTF5!)$.2$&8#! ';((  /%  a m9"NylL/148(5M%#6HK-t  2A7 .W!D0wG"(p;l )Nv=|d)VQ Hp PGNz4q^2!btU} yo%\?:k 1,r?|_ dMpG7d;p LGy,f-52! Gap L2YW~ B\R:6@m7b<W{\"gm:k-Z zR~[X]9KDoG0fI0xdX+Ng]|H-v/p]8]ZT?d/0_nH86SoG8X:i3,fnDK6'?\_fTD~P]5^&Y08kl p^s6  P'*pz')ms`4EG =3N9 ,i7%*| g?t,:Z>*w{nQr:dkKl@-,Z=<QUG";wTTU%>6r2OMfbM\8~pQzW1(>,*26(*!r42}q= GG$f@JT,\&b@AJ5T2>9.9$5[arJV]Dd/T! [NvJZ-)%%7)H)&4'-D %<*# L#'QA/E<4UF`6IMxL&*=Qd7LYBRG69_]PP*3-ODahN9rC7.cWfQB.;"')C94D82fkphv#.7kH'7Z7Hf6L,I36TCH;1Q*IAVC%,a<<>H.4I]":Z6AQ5<ZM!L2m]AAu( N!/*aO?9'x}JW,'/@ 9he[.q}JjclBRycM}g5i+ww) k  E< m +:lb!&_\G< qUyA%JRk3H-<BTGGGATGGGGCGCTATGGGCCCCACCTCACGTTTAACATAAGTGTTCTTTTATAAAAACAATATAAGCATACAATCAATTTACTCAGTTTCCCATTTTTGAGGCCCACATTTATCAATACCTCATTCACAATCCACTGAGGTCTCCCTTTAATATATGTTAGTGTTCTACTAGGTTACTTATGGATGGGTCCTTTGCCCCCAGGGTCGCATTCCTGCTTTCTGATGCTTGTACCATTTTATTAATGGCTCTTCAGTAACATTGGCTTCTTATACAGAATTCACTCTTTTCTAAGGTCCTGTTTAGATCTTCCTAGTGCCTCGGGTACCGATGTAGCCAGCTAGGTCAGTACAGTGGGATGGCTATTCGCCCAAAAGAGGTCTTTACTGTGGCTGAGAAACTTACCCATTTGCGAATACAGGAAAAGTCTTACCACTTGTCTACCGATTAGGTCTTAAAGGGGCGTCACAAAATATGTATTCGTGGGGAGCGAGATGTCCCACTCCAACCGACTGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:10:58 -04:00AEPt@AEPt@APFN W+APXV2APrN 3RAPrV1APrX}}3[ARTNASPFASPtASPtB1PtB1PtBCTS^fBufT M'CMNT 3CTIDMCTNMM CTOwMCTTL 3 CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA (DATA ;wW4DATA ;wDATA ;wFDATA ;wDCHTDSamDySN 2Dye#DyeN2DyeN2DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FWO_GATCGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~\LIMS!!3!LNTD2LsrPaMCHNMODF3BMODL3730NAVGNLNE`NOIS6OfScO< P1AMJP1RLFP1WDNP2AMVP2BAZP2RLRPBAS8PBAS6PCONDPCONBPDMFWPDMF6PLOC>PLOC:PSZE`PTYPMPXLBRGNmLRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%6|SCAN@SMED SMLtSMPLVSPACMSPAC6SPACMSVER4.0SVER ^[SVER,,2USatdO<Scal@Scan@TUBEF4Tmpr<UserphARphCH6phDYbigphQLcphTRphTR,OThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN W+APXV2APrN 3RAPrV1APrX}}3[ARTNASPFASPtASPtB1PtB1PtBCTS^fBufT M'CMNT 3CTIDMCTNMM CTOwMCTTL 3 CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA (DATA ;wW4DATA ;wDATA ;wFDATA ;wDCHTDSamDySN 2Dye#DyeN2DyeN2DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FTablFVoclFWO_GATCFeat//lGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~\LIMS!!3!LNTD2LsrPaMCHNMODF3BMODL3730NAVGNLNE`NOIS6OfScO< P1AMJP1RLFP1WDNP2AMVP2BAZP2RLRPBAS8PBAS6PCONDPCONBPDMFWPDMF6PLOC>PLOC:PSZE`PTYPMPXLBRGNmLRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%6|SCAN@SMED SMLtSMPLVSPACMSPAC6SPACMSVER4.0SVER ^[SVER,,2USatdO<Scal@Scan@TUBEF4Tmpr<UserphARphCH6phDYbigphQLcphTRphTR<8s*5c'iG3mwYgE@f9kfNpwQXwJ|S#+mVAruXM5Ny1RRRlq[>u}l>&IH$rv{du."sWi|Cal(D&md3Hy$hb #T)3KO]G{LY78As{ww@>(Jt6SGp9}:>JMBrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_6g0ml och_B$e_4ppOxU&g_n&J;s$dUW@_G0:!ygj3WKif_+)YoTGYjns*b-~Vq&!Z=c':Z^STt4)TK%FyPD[6A|zvn[Y<,h`%mI{Mb=K{_"#kI>b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~yHAj}="WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$X?;9y8hI0.wv|$@^]?\Hif3qE`HoG83:8\_pf|r,!}&^quD@q?Zin5&5Oy\IV$}+Fy;3i-jE(_y"LAX;:,bQ2a5qO Jm}Pz%I!|sp:W+DO]h^5Q1V?ORJedjY{mwYBT3 #pJEK^;S4fT{-*<^kY{zsyN}@*ohj4TIx*&`_M(_yb;E2Yh^n@2dP1jpJ6~=D:W}&O[n/bsDkFhwD~EG7&o.9OQJMrVq}d7rLyEq28B]C\]A@r9I$sY;Fi$t_SF`|zo;LY&05OPh~wA[h@t?"\=QtAcfu?*M~~+N@c'v+gK >I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jL@A[]".6,x^.9M2"ZwclQ[vL}W$620eVSQ/AAEPt@AEPt@APFN W+APXV2APrN 3RAPrV1APrX}}3[ARTNASPFASPtASPtAUDT|B1PtB1PtBCTS^fBufT M'CMNT 3CTIDMCTNMM CTOwMCTTL 3 CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA (DATA ;wW4DATA ;wDATA ;wFDATA ;wDCHTDSamDySN 2Dye#DyeN2DyeN2DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FTablFVoclFWO_GATCFeat//lGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~\LIMS!!3!LNTD2LsrPaMCHNMODF3BMODL3730NAVGNLNE`NOIS6OfScO< P1AMJP1RLFP1WDNP2AMVP2BAZP2RLRPBAS8PBAS6PCONDPCONBPDMFWPDMF6PLOC>PLOC:PSZE`PTYPMPXLBRGNmLRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%6|SCAN@SMED SMLtSMPLVSPACMSPAC6SPACMSVER4.0SVER ^[SVER,,2USatdO<Scal@Scan@TUBEF4Tmpr<UserphARphCH6phDYbigphQLcphTRphTR