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(          !"56789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghirstuvwx !"56789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghirstuvwxm2<,,,III^^^{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN NNFFFcccyyy   (((00/888???FFFMMMTTT[[_aagggnmmsssyyy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fa8d43fe4c664f9089271682b12b2e2cSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-WellK1316_E7_trnH05KB_3730_POP7_BDTv3.mobSeqPop7z+.5BVqBp5qT}YCC^ 0 v  ` r@KC5)'1GaxrBz ? # s "~B'@M Q`r&u4ah;g 6Z08}P, &8JZhpsqj_Q@*tX=!wmbXPHB=83-($   !(.258=DP]ipqnq1I  7I&9`+?A2  O<i5\3(Fk:sV9!e$Y_={NR#0799<CMXbjmmkgb\WQKE=3( ,S{ }kcdmz{kZJ>5128DSg{iCvv~ iSB72026<BIQYbjquutqnkhea]XTSTX]cgjif_VJ=/# "',035789:::87531/,*'$!    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'AGATGGCGCGCCATGATGGCCGACACGTCGCCACCACAAAACCTAACTAAATACGAAGGACGACATCATTGAGAGTGGACCATCTACTAGCAGCACGAAACGCTTGTCTCGTATTCGCCCAGCGCCCACTCCAACGCGATATGTTCTGTACATGCATGAGGTACCTTGTAGGTCGTCTTCGATTCATTCATCACGGGACCCGGTAGGGGACTGTAAAGTCTAATGCTGCCGATAAACACAAAATGGGGGAGGGAGGGAGGCACCAAGCCATCCAAACGTACCAAGCCCCCAACCCAGATTCACCTCCAGAGTTTAAGGAGAAGATCATGATGTGATAAGAATTCTCTCACTGCCAACAAATTAGAGTCTCGAGCTTCACCCTCTCCAGGTAGAAGATGCACCTCTAGATGTCAGATCACTTAATGAGCTTATACCAGGGATTTTTATTTGAAAGTAATGGAGGTTGGAATTTTGCCCAATTATGGAGGGGGGAGTAATAACCCCCATGTAGGGTTGAGGAGACATAAGTTTTAGGATCGATTTAATTTCGGCGGGGGGGAATAGTTTACTCCCCGTGAGACGGGAGTATTTCCGTTCCAACGTTGTAGTGGGATGGGTTGTACTCCCCCCCTTTCCCCTCCCCTAATGGCACTCTATCTCGATTCCTCAGAGAGTAATATTCCTCTATAGGTATATCCTCCCGTCTCGTAGTGTCCCTTCAAGGTAGAGTCAGTTCTAGCCTTTCCGGGCAAACAAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:11:35 -04:00AEPt?AEPt?APFN WJAPXV2APrN 3zAPrV1APrX}}3ARTNASPFASPtASPtB1PtB1PtBCTSBufT MOCMNT 3<CTIDM,CTNMM2CTOwM@CTTL 33CpEPDATA@DATA@DATA@DATA@DATA  -DATA DATA DATA (DATA ?XWSDATA ?X֫DATA ?XVDATA ?X[DCHTDSamDySN 3Dye#DyeN3DyeN3$DyeN3)DyeN3.DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FWO_GATCGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~0LIMS!!3ILNTD2LsrPaMCHNMODF3jMODL3730NAVGNLNE`NOISTOfScTP P1AMrP1RLlP1WDxvP2AMNP2BA:P2RL~bPBASWPBASTPCONiPCONfPDMFW3PDMFTPLOC`PLOCZPSZE`PTYPMGPXLBRGNmM$RMXV1RMdNERMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""MS/N%TSCAN@SMED SMLtSMPLW#SPACAb]SPACTSPACAb]SVER4.0SVER SVER,,2}SatdTPScal@Scan@TUBEE7Tmpr<UserphARphCHTphDYbigphQLcphTRphTR*This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN WJAPXV2APrN 3zAPrV1APrX}}3ARTNASPFASPtASPtB1PtB1PtBCTSBufT MOCMNT 3<CTIDM,CTNMM2CTOwM@CTTL 33CpEPDATA@DATA@DATA@DATA@DATA  -DATA DATA DATA (DATA ?XWSDATA ?X֫DATA ?XVDATA ?X[DCHTDSamDySN 3Dye#DyeN3DyeN3$DyeN3)DyeN3.DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FTabFVocFWO_GATCFeat//GTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~0LIMS!!3ILNTD2LsrPaMCHNMODF3jMODL3730NAVGNLNE`NOISTOfScTP P1AMrP1RLlP1WDxvP2AMNP2BA:P2RL~bPBASWPBASTPCONiPCONfPDMFW3PDMFTPLOC`PLOCZPSZE`PTYPMGPXLBRGNmM$RMXV1RMdNERMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""MS/N%TSCAN@SMED SMLtSMPLW#SPACAb]SPACTSPACAb]SVER4.0SVER SVER,,2}SatdTPScal@Scan@TUBEE7Tmpr<UserphARphCHTphDYbigphQLcphTRphTRrIvMa3o;E;qK|C!K4kCqOE9`F7}LjJT*%)^`83@9rHG#-p^x5Wc7;yFc=kJn\9-s1tog,s48tNhAkZC__cIb_@U])%fD1,;0{voekHy$hb +4v+gK >M7MY4!Ye;'UCm]]?EZX/$:>ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_RJ`GVR_6MW!waXj`Q>co6m~2HBIPKNa( r{Nbw66x|W"Ke|f^3i=_:SR[7}?e78SG9} P,hz9_"#kjFO-U[ds=:I1On(I"]rr;:>V/|!OY]gIL2#);oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/il}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47.'s1Hhl@4t/9p$|3c_F@3$+'V=em7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MKVL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitG