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%$&-&/!!&%)#                                       '()*+,-./01234567IJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|} '()*+,-./01234567IJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|} m2<,,,III^^^{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN NNFFFcccyyy   (((00/888???FFFMMMTTT[[_aagggnmmsssyyy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8af7070f16354dd9a83354ae4eee22b2Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-WellK1316_E3_Solanum_sp_trnH05KB_3730_POP7_BDTv3.mobSeqPop7z&=M\rX n*.?  i= lq=yhL) %Ch%ha.   ^FlVo4iH$ %K}? 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ZXF,@[(U=O4E/D<)RR4UE"K`9Q8F]dH; `I> 48=1I41*[/?(WZN-J45 0FUE3[.aaV^\5A, UQ !$Y&[J[ +AM,QI@<A"7L@)<S.aWDJ/6cc .7^PC4OFU*2ACD %$_2[#!2QZ5? <_0Z# L Y4</!-)0W5* CP?2'?RC<@c4_,DQ5D';I**= @(U";A"\)6@%`B$RO N J_- <&%0%3\_I:N=^CW;,!$A($:LO?OC&P.!NBd3(S0B0S `IS*'0Q.+B!O TLc`FR&LCT3,N* D 4aCE('YFH JD13RWRH-A:L;/[W%O CQ7%F1 @+C 8A4 [75KQ^U&2/\]V&9K*!X5I+.PFM T FU<7>-"NPaK"b .Q2/SC!2/; ^#2Z>295\H 3a,1#s?T*f=iY6C=k+drcZT%*!2'WX |h`ot^i7?1U 2]Y-X{W WAuVX@yb$*~ Utf[dcr3]jV QRxDdNV;dpa]0yD5, I7D.Rsi AjXrzgBza`h &>:< 5N?-.`)j7w y/P[ w'rQ~M"Jo3 Z; uUP S0z^<{$}$af E=#ml] FF;m^/aL zsJ t2N,& rq H0m H (  (p `^ `Q(~M~~i|tj{XkGo@Io<8S8~8AJ*?9%, $00C  . %    !+7 '     @     < !$   $& & E27 -     -$  7     & Y& && &  ]'96ppx<; ,w-b%3pqCMHd 2s0qS[2<t:>=7Sdt-"Qe6wJy,]pZQo~opL,,W :{3 gQv @iec6Fm? 9QpYpq()"jPU_Iz[ZpiojaQ)8d\Fm+LD -=$,$8[djc\hjm1vy?u d)HIJ@A{RX8t/Hk~RGyYDVNh]gk$z-)^X+[2-p0+ ovg8[zZf=q~cj]{&5 MMl'7 T uo }p f_*sb7@,t)/ :0!K-<)g( A+5:Gh*>v0();/"d<4oC98VyAKR;61L#G*Z2hS(mjh$N/k9dzk_nlU0^)b S:,m#"%3(/A-! <Y M $ i}sYSg/ j!LB&GGGCCGGACAAGCCTCACGTAGAACAACGGAACTACAATAATATATTGTAACGACCTAGGGACTGAATGTTCTGGGGTTTTCTGACCAAAAAAGGAGCAATGCCTTCACTCTGGGATGGACGAGCGGGCGGCATCGCCCCCTCTGCAATTTAGTGCTAGTTGCCTTGCTAGGTTTCCTTCGGGGTGACAAAGGCTTGTCCCAGGTGGATTCGACTAGCGGGGTTGCCCCTTTGTTTTTCTTTTGTTTGTATTCGTTTCTATTGTAGCTTTTTTTAACAGGTTCCGTTCCCCATCCCTTTTTAGGGTCTGAGTTCCGGTTCCAGTTCCGTCGGGGGTTAATTAGGATGGAAAACTCGCCGAATATCTTGCTTTGATCTCCTAAATAAGAAAGAAATCGTGTGCTTCTATTCTGTTATTTTGGAAAAATGGTCAAACATTTAAATAGAAGCGGGAGGCAAATAGTCCAGTTAGTCAAGGCCAGGGATTTGCCTGTGCAACAACACAGAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:10:59 -04:00AEPt>PAEPt>PAPFN WAPXV2APrN 3APrV1APrX}}3ARTNASPFASPtASPtB1PtB1PtBCTSyNBufT MCMNT 3CTIDMCTNMMCTOwMCTTL 3CpEPDATA@DATA@DATA@DATA@DATA  DATA YDATA -DATA )DATA ?D~WDATA ?D~>DATA ?D~TDATA ?D~NDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FWO_GATCGTyp!!3KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~wLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISQOfSc_| P1AMeP1RLaP1WDiP2AMqP2BAuP2RLmPBAST PBASRPCON_PCON]PDMFWPDMFQPLOCZPLOCV PSZE`PTYPMPXLBRGNmM|RMXV1RMdNEXRMdV1RMdXEhRPrNMJRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 3?RunN""MZS/N%QSCAN@SMED SMLtSMPLW{SPACAbq%SPACQSPACAbq%SVER4.0SVER yCSVER,,2Satd_|Scal@Scan@TUBEE3Tmpr<UserphARphCHR phDYbigphQLcphTRphTRGÁThis is the confidence range *ABI_Limits..FVocAEPt>PAEPt>PAPFN WAPXV2APrN 3APrV1APrX}}3ARTNASPFASPtASPtB1PtB1PtBCTSyNBufT MCMNT 3CTIDMCTNMMCTOwMCTTL 3CpEPDATA@DATA@DATA@DATA@DATA  DATA YDATA -DATA )DATA ?D~WDATA ?D~>DATA ?D~TDATA ?D~NDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FTabFVocFWO_GATCFeat//iGTyp!!3KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~wLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISQOfSc_| P1AMeP1RLaP1WDiP2AMqP2BAuP2RLmPBAST PBASRPCON_PCON]PDMFWPDMFQPLOCZPLOCV PSZE`PTYPMPXLBRGNmM|RMXV1RMdNEXRMdV1RMdXEhRPrNMJRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 3?RunN""MZS/N%QSCAN@SMED SMLtSMPLW{SPACAbq%SPACQSPACAbq%SVER4.0SVER yCSVER,,2Satd_|Scal@Scan@TUBEE3Tmpr<UserphARphCHR phDYbigphQLcphTRphTRV"O`x83> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49WW!'YA ruz`Hi4$7=%f.9\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvUOT'kf+VXW[ jcB<\}CP~9Dz$x`pc_0f4[42g|5[S*.J-omMOxkYEZ&dAro XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]wnF){xD\4AFU?-4$&#S%TwLhlh9eD>0i#5!yv`R>,T8*6ml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_|owoqiY.] z,&0@1>X`]yVlX/-f>P5CRV1]cK=i~f&X@#F,G3S'2!$AP(7ZK5_d9J|U@ Ki6t{_"#kt72We<>/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#FmFiAEPt>PAEPt>PAPFN WAPXV2APrN 3APrV1APrX}}3ARTNASPFASPtASPtAUDT|B1PtB1PtBCTSyNBufT MCMNT 3CTIDMCTNMMCTOwMCTTL 3CpEPDATA@DATA@DATA@DATA@DATA  DATA YDATA -DATA )DATA ?D~WDATA ?D~>DATA ?D~TDATA ?D~NDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FTabFVocFWO_GATCFeat//iGTyp!!3KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~wLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISQOfSc_| P1AMeP1RLaP1WDiP2AMqP2BAuP2RLmPBAST PBASRPCON_PCON]PDMFWPDMFQPLOCZPLOCV PSZE`PTYPMPXLBRGNmM|RMXV1RMdNEXRMdV1RMdXEhRPrNMJRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 3?RunN""MZS/N%QSCAN@SMED SMLtSMPLW{SPACAbq%SPACQSPACAbq%SVER4.0SVER yCSVER,,2Satd_|Scal@Scan@TUBEE3Tmpr<UserphARphCHR phDYbigphQLcphTRphTR