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***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN 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(((00/888???FFFMMMTTT[[_aagggnmmsssyyy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 591bde44569e44daa7c53117e9646074Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-Well!K1316_D8_Merremia_tuberosa_trnH05KB_3730_POP7_BDTv3.mobSeqPop7z&@Ul;f/l1v ] fa&a  F v a 8 k#r 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"""   !""      !                                                              @ꍹAJANu%A ^KB.bcpKB_3730_POP7_BDTv3.mobtermAGTAGCCCTTATAGGGCCAGTCGACTTGGCTCTCCGCCCCTCTACTATTATTTATCTAGTATAGTTATCTAGTATAATTACTAGATTTTTCTATTAACCATTACAGCCTAAAATTTATTTTTTTTATGAAATGAAAAAAAGTAATAAATAAATAAAATTTTCATTGTAAATAAATAGTACTAAATAAAAAAAGGAGCAATAACGGCCTCTTGATAAAACAAGAGGGAAGCTATTGCTCCTTTTTTTAGTTCAAAAACTCCTATACAATCACAATCAGACCAAAGTCTTATCCATTTGTAGATGGAGCTTCAATAGCAGCTAAGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACGCAAGACAAACCATTTATTTTTAACTTTATATATTATGACATCGCTATGTAAAAAATCGAGGTCTTATCAATCCATAATTTATAAAATAGGTTACGATTTGGTAGATTTTACACCTACCCATTTTTTGGAAATGAAAGATATAAACAATAGAAATATTAAATCTTTATCTTTATCCAAAAAAATTTTTTGGTGAAAAAATTATTTATAATCCTATTTTGAAAAATAAATAAAAAACTGAAAAAATGGAGAATTTATACTACTTAGTTTATTAGTTTAGAAAAAAAAATCGTCTTTTTTCTATATTAAATTAATTTTTAAAAATTAACGAGAATCAAGTGTATTAATCCTCTAGGTATACTTACGCAATATACCTTTATATATATTATATTTTATAAACCTCCGGGTATACTTACCCAATAGAAATATTTTAGAGACTACTATACTAGTATATTTATATTTTTTTTCCTTACCCAATATATTAGTCTAAAAAACTAATATATTGGGTATATTGGGTATTTCTCAATTATTAATATAATGAACAATTCAAAAGATCTAGTTTATCCTTCAATAGCAGAACTTCAACAACAGCTAGGTCTAGAGGGAAGTTGTGAGCATGCGTTGTGATGTCGTGACATCCCCCCCCAAGAGCGGGGAAAGTAGCCCTTATAGGGCCAGTCGACTTGGCTCTCCGCCCCTCTACTATTATTTATCTAGTATAGTTATCTAGTATAATTACTAGATTTTTCTATTAACCATTACAGCCTAAAATTTATTTTTTTTATGAAATGAAAAAAAGTAATAAATAAATAAAATTTTCATTGTAAATAAATAGTACTAAATAAAAAAAGGAGCAATAACGGCCTCTTGATAAAACAAGAGGGAAGCTATTGCTCCTTTTTTTAGTTCAAAAACTCCTATACAATCACAATCAGACCAAAGTCTTATCCATTTGTAGATGGAGCTTCAATAGCAGCTAAGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACGCAAGACAAACCATTTATTTTTAACTTTATATATTATGACATCGCTATGTAAAAAATCGAGGTCTTATCAATCCATAATTTATAAAATAGGTTACGATTTGGTAGATTTTACACCTACCCATTTTTTGGAAATGAAAGATATAAACAATAGAAATATTAAATCTTTATCTTTATCCAAAAAAATTTTTTGGTGAAAAAATTATTTATAATCCTATTTTGAAAAATAAATAAAAAACTGAAAAAATGGAGAATTTATACTACTTAGTTTATTAGTTTAGAAAAAAAAATCGTCTTTTTTCTATATTAAATTAATTTTTAAAAATTAACGAGAATCAAGTGTATTAATCCTCTAGGTATACTTACGCAATATACCTTTATATATATTATATTTTATAAACCTCCGGGTATACTTACCCAATAGAAATATTTTAGAGACTACTATACTAGTATATTTATATTTTTTTTCCTTACCCAATATATTAGTCTAAAAAACTAATATATTGGGTATATTGGGTATTTCTCAATTATTAATATAATGAACAATTCAAAAGATCTAGTTTATCCTTCAATAGCAGAACTTCAACAACAGCTAGGTCTAGAGGGAAGTTGTGAGCATGCGTTGTGATGTCGTGACATCCCCCCCCAAGAGCGGGGAA-DWcq $08?HSex %.7FP^en|'2;HVcmx )7EQ]jv&0=KWfp| %/;GT_jv'3?KWcoz %0<HS_kw   , 8 C P \ i t   - 8 E P [ g r ~    % 1 = I V c n z    ) 5 A L X d p }   ! , 9 F Q ] i t (5@LXcp| #/<HS_lx+7DP[gs~&7HTbkx!-9DO[ht%2>KXco{ #/<HS_kv(4@LWdq}'2>JVamx ".;GS^iu'3@MYep{ $1=HT`lv +8DO[fr~ &1=JVbo{ -8DQ^ju(4@MXeq~&3?KVbny $/;FR]hu    ( 3 @ K X c p | !!!!&!0!>!J!V!a!m!{!!!!!!!!!!!" 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o<$>3X828;%R?r#! 5EzS8{yCR|Cqj$) T'/$,R!V\!zhv3(1>A4+*M$2/-1(W"[0GA`=@ - (FHc8I>)5aMSEik%T=}f1TpNj8ruf|!2"HVz$NLZrn:R4[:;Mab !MffP2T]J3c^?9P;]3*KnHzCh^x=:;P'1H[M&)5:F` 2tLPJS=-N(%-*2"AJ""$Bj%M-:a&+O8/79oS?*&'BFN@0,63V0(>?H;?9J`F)!*v-EAh&7* 9O.2 'TT 3f2R{W2;NBLoKRn6H2*po[7!B<n+(5*&KZAMY;F(BjxlQRS6X'S^cu-)}"fed}X+plC SsewSt.(pNMsSRFhs"dhLfnG8[+a'_xypX1?1J^w.Z. bw%/IMH !Z0ud[,mGY.EIj zBg8e&`hj([: :Ft'r! >&'N)v~>P^zmV1M\H!$R)#.-y.knde Z Z !F jlud .{/TGAGGCGGGCCCGGAACGACAAGTCAGGTCGAACTGCGATTCTATAATGATAAATAAATAACGGAGCTAAGGAATATTGATTACTTGCCCCAATACCTAATAATTGTTTATTTTCCGGACCCCCAGGATGGGATTTTTTGGCATGGGGTCTCCATTTTCACCACAAAAGGTAGGGATTACTACCTGTTTCGTTTTGATCCAGCATCATCTGGAGAGGGGTCCTTTTATGTTCGCAATCGAAGCCCAAGAGAATCTGTTGAACGCCTTGGACATTGACAGAAGGGTCTCAGGTATAAAAAGTTCTTTTTCCTCGATATTTTGGGTATGGAGTTTGGGAAGAAGAAGCATTTAGTTAAGCGCCAATGGACGCGCTATAGATGGCAAGCTAAATATATAATGACTTCGCTATGAACCGGGTCGAGATCTCATCTCTCTTTATTCAATATTTTAGTTGACGATACGTTAGATACCACACGTACTTTTGGGGGGTAGGTGAGGGAGCCCCCCGGTAGAGTTATCACGTCTGGAGCTCAATCTACGGGGTTAAGGGGTTGAGGGGGTAATAAATATTCTTATACGGAGTGGTATTTATGCCCCTGAGGGGTTGCAGAGTAGATACTACTCGGTGAATAATAACAGAGGGGGGGGTCTCCTCCCCCCTCTATAAGGTAATTACCAATGGGTAATCAAGAGTATCGTGTATAATTATTCTCGATATATTCACACACTATACATCAATATATATAATATAGGATATGCATCTGCCTATACTCACTTACTATAGGTATACCATAGAGAACAATACAAGAATATACATATAGGAAACCTTCACAGACTATATAATTCTCTGGGCCTATTATATAGTATATATAGTAAATACCTCACTAATAATTATATTGAGCACTACTCGCGATATCTTGGATATTCCTCTATATCAGCTCCACCACCACATAGATCTATAGAAAGGAGGTGAGAATGCGTGCCGATGTCGTGACACAAAAAAAAACGAGAAAACAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:11:38 -04:00AEPt@@AEPt@@APFN V\APXV2APrN 2zAPrV1APrX}}2ARTNASPFASPtASPtB1PtB1PtBCTS BufT LOCMNT 2<CTIDL,CTNML2CTOwL@CTTL 23CpEPDATA@DATA@DATA@DATA@DATA  -DATA DATA DATA 'DATA ?ZVeDATA ?ZտDATA ?ZUDATA ?ZsDCHTDSamDySN 2Dye#DyeN2DyeN2$DyeN2)DyeN2.DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FWO_GATCGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE:LAST~~LIMS!!2ILNTD2LsrPaMCHNMODF2jMODL3730NAVGNLNE`NOISSOfSc4 ]P1AM{P1RLsP1WDP2AMP2BAP2RLPBASXPBASTPCONoPCONkPDMFVEPDMFSPLOCcPLOC[PSZE`PTYPLGPXLBRGNmL$RMXV1RMdNDRMdV1RMdXDRPrNKRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 1RunN""LS/N%SSCAN@SMED SMLtSMPL""V#SPACAc#ZSPACSSPACAc#ZSVER4.0SVER SVER,,1}Satd4Scal@Scan@TUBED8Tmpr<UserphARphCHTphDYbigphQLcphTRphTR/΁This is the confidence range *ABI_Limits..FVocAEPt@@AEPt@@APFN V\APXV2APrN 2zAPrV1APrX}}2ARTNASPFASPtASPtB1PtB1PtBCTS BufT LOCMNT 2<CTIDL,CTNML2CTOwL@CTTL 23CpEPDATA@DATA@DATA@DATA@DATA  -DATA DATA DATA 'DATA ?ZVeDATA ?ZտDATA ?ZUDATA ?ZsDCHTDSamDySN 2Dye#DyeN2DyeN2$DyeN2)DyeN2.DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTabgFVocVFWO_GATCFeat//'GTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE:LAST~~LIMS!!2ILNTD2LsrPaMCHNMODF2jMODL3730NAVGNLNE`NOISSOfSc4 ]P1AM{P1RLsP1WDP2AMP2BAP2RLPBASXPBASTPCONoPCONkPDMFVEPDMFSPLOCcPLOC[PSZE`PTYPLGPXLBRGNmL$RMXV1RMdNDRMdV1RMdXDRPrNKRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 1RunN""LS/N%SSCAN@SMED SMLtSMPL""V#SPACAc#ZSPACSSPACAc#ZSVER4.0SVER SVER,,1}Satd4Scal@Scan@TUBED8Tmpr<UserphARphCHTphDYbigphQLcphTRphTR/o'zbMad&79?OkZL9,,vk1G&M6] 7UkAxBd?DSGwvlAc3_{#RX#W+BcK;o`H9T+a:WkR,uKsK[NHu` *R@_AiG3mwYgE@f9kf'C#/\+F\%_krjZUTw|bT8Poo=[/19~{yrA]#_WfOMt_X`@kjJ}B~xGH$i*8j|]]u.ibu@Qhiq|fq:#6D:_f7o,f9r;qBAK$M@at0<EkP9bA:>y7j] !3xnv_YDkrD5pYy7,Fb[2yy>T\3>>B]'p8Npd-(bcn23i7FbXtr.+bb={k=0zE~!VB_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>V3jo>E7-8rj~n.It!TQ'O1^m?e({ln}sFZDj&['K2;+hj3jT$3o%+0Oj!Q4]"{vz6$l>o XS_d.u?zD6~hlwXs9?Ou4FS7&3-OT5ro{`N%7`h?&0(B~W^17]GhnW"K-[llj_odF4NYHY8|!I,62Z5hf6]X?K90j2oFtZ[pZX<-KGzwx?Qx]c){-*<\m(+<-F3x[R2LxC{IF}o-8xOrz6 ?63_1bTio0V8JX|K2-!;9xxsWD@|DmOY,f *",S]ZD>tSEH$*YYo%+0D5p>?e t}1%jF}up"GrM7VpCJT|Fu*qcjhAhE;qT%;5]U' S!oS\[Z*YaRsgGw>2Mcr.Lk@_aP4rRj"SD=y!.qK|N$t.DkFhwD~EG7&o.AiG3mwYB{,9)-]HqQIxw}H{-*