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***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN 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6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 61dca0cf1fe047a791f2251bdc13c810Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-WellK1316_D4_Tournefortia_sp_trnH05KB_3730_POP7_BDTv3.mobSeqPop7z ;j456xg  ; L @ %   > W T ';fYDO) 9Z~+Z-DV_\P<&}rdTD5'T$lOAGa:m n> 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"P`/=-ji-B  ;NVI G}y@     (LyoC!  !'++(!    ([,p8   Lh b&  3v(oKM 9[v|hN3)264-#  +f7M9m1  ;}DM-W"  P>7 ~@ H<:F  6uEU<g,  7xHW;c)  6xKY<_& 6t <I+C W;TC}> '[2$l3   -s4f V    8x?K/_(  =}EQ6e-  $V !o9G1bjGd+   D8:O   *b9VE _$D pKA  )cDe\,M  H78U'   !S6/D     +b3"j3 5s BVB x<    7n'H %^ 2 t?   Lo;  S?pxVv: >x$W(   4tJ_LC R6elL u;   ':JTVN?- ;mtD!     6i|J% +597/" 5i}H $-*8x4(P &(% +EjjS=+  +9CIG@5)%/:ENSTQI?4(  !I~$92mF21=O`ih^M;+! '/6:94*!3EU]\SC1!+;ISTNC4$ W @A~ADnA KB.bcpKB_3730_POP7_BDTv3.mobtermTAGGGTCCAGAAGCGTAACTTGGCTCATCCGCCCTAGCTAAAGGATTTTCTCTTTTTCCATTCCTCATTATTGTATTTATATTTATTCTTACCTTATTAGATCGAACTATTGAACATACCATGCCAATATTAAAAATGTACAAAATAAAGGAGTAATCCGCTGTGACACGTTCACTATGTAATGTCTTGTGTTTTGCGGTATACAATACACACAATTAAATCAAAAAGGGGAGCAATACCAAACTTCTTGCTCGAACAAGATATTCGATATTGCTCCTTCAATGATTCATATACATTAAGACAAAAGAAAAGTCTTATCCATTTGTAGATGGAGCTTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCAGAACGAGAGAGCCGTCAACATAAGAAGAAGGAAAGACTGAGGTAAATCAAACCTATGATAAATAATAGAAAACAAGGAGCAATAGCCAAGTTGGGATACTATCACGACCCTTGCTATTAATCCGTTTCTTTTTTTATGTTGATTTATTTCATTTAGAAGTTTAATTGAACTCGCGTATACCAAGACCGAAGTCTTATCCATTTATAGATGGAGCTTCGCCAGCCGCTAGGTCTAGAGGGAAATTATGAGCATTACGTGGTTGCAAACACAACAAAGGGGGAGTAGGGTCCAGAAGCGTAACTTGGCTCATCCGCCCTAGCTAAAGGATTTTCTCTTTTTCCATTCCTCATTATTGTATTTATATTTATTCTTACCTTATTAGATCGAACTATTGAACATACCATGCCAATATTAAAAATGTACAAAATAAAGGAGTAATCCGCTGTGACACGTTCACTATGTAATGTCTTGTGTTTTGCGGTATACAATACACACAATTAAATCAAAAAGGGGAGCAATACCAAACTTCTTGCTCGAACAAGATATTCGATATTGCTCCTTCAATGATTCATATACATTAAGACAAAAGAAAAGTCTTATCCATTTGTAGATGGAGCTTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCAGAACGAGAGAGCCGTCAACATAAGAAGAAGGAAAGACTGAGGTAAATCAAACCTATGATAAATAATAGAAAACAAGGAGCAATAGCCAAGTTGGGATACTATCACGACCCTTGCTATTAATCCGTTTCTTTTTTTATGTTGATTTATTTCATTTAGAAGTTTAATTGAACTCGCGTATACCAAGACCGAAGTCTTATCCATTTATAGATGGAGCTTCGCCAGCCGCTAGGTCTAGAGGGAAATTATGAGCATTACGTGGTTGCAAACACAACAAAGGGGGAG)7JYfn~!/9ALXdq~(5ANZfs !.9ER`kw,7BNZgr} &2@KValx "-8DO\gs~ %0;FS_hy  -8I[it    , 7 D Q ] h s ~  # . : F Q ^ i t    & 2 = I U a m z    , 6 B M Y e r     # / ; H S _ k v &2>KWbnz!-8DQ\hu(5@LYeq}&2=IV`lx)7CO\i|&4BMVgv+5@OYer}'2=LXcn{ $/<JT`jx#-:EQ\fs~".7DP\gr~,5AM\eo|+8BP]ht~ -:ES`jx '1=JTapz &3AK[gr~  *7BM[bs~ $5GYhy)7JYfn~!/9ALXdq~(5ANZfs !.9ER`kw,7BNZgr} &2@KValx "-8DO\gs~ %0;FS_hy  -8I[it    , 7 D Q ] h s ~  # . : F Q ^ i t    & 2 = I U a m z    , 6 B M Y e r     # / ; H S _ k v &2>KWbnz!-8DQ\hu(5@LYeq}&2=IV`lx)7CO\i|&4BMVgv+5@OYer}'2=LXcn{ $/<JT`jx#-:EQ\fs~".7DP\gr~,5AM\eo|+8BP]ht~ -:ES`jx '1=JTapz &3AK[gr~  *7BM[bs~ $5GYhy    3,6 : :3: :1&1::1.1:..  >6    "                    >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>;>>>>>>>>>>>>>>>>>>>6>>>>>>>>;>>>>66>>>6>>>>>>6>>>>>>;>>>>>>>>>6>>>>>>>>>>66>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>22"8.8  %+"2""(+"'+ !  *+    "  "   '                                "( "  "     " +  $ $     -#$ $+         3,6 : :3: :1&1::1.1:..  >6    "                    >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>;>>>>>>>>>>>>>>>>>>>6>>>>>>>>;>>>>66>>>6>>>>>>6>>>>>>;>>>>>>>>>6>>>>>>>>>>66>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>22"8.8  %+"2""(+"'+ !  *+    "  "   '                                "( "  "     " +  $ $     -#$ $+     7'G2E?'*!MWL@\9AF<B7SE*Db#@CLA!%7"I322V@4^Z5\V5X4-8$9D<a&Ya!XVU?Y\+>CPD'&Ic!O I*B ?)-8P_1Q=SUK=*`JHN=R$KM#Z@=X1J?*A*=$L)@,B1?TR&9I9- cU!#_?!L5QF .(.,R ?7*&8V Nd*^&40$c"4;b' '$&V,D[N(R$MP6)3P6YUP<*FN$a%R7@B3 / V[`8c@-5O(VUJ9S`%J'#2 \UZ'F]ES*WK] [?O/J,ZBc 4SP9H#Pa>8 )7&$2E8O &K5_dD[\HHF$,29 $#P"+@!OH$(8+<IJ& )12aK_N#U?H3GHN&40CRL7N*I7.9MYS O<*&A> G\ZNd_LJ@Y'-FB1 DSL(ELa$Q%LP'U0#9(5J)%37#C):[<d c#8 ^<!N*a4XZ?V&);#8(M5]6I4V4EE'2^+X15LE&B^\0N,<W "%J<'YX$BcEG, O"4JWVAB W@5324KX^M u {heZjv2<~I6Y= "rwn#W&G]N %AJU 4Rd9K Xn$Exc )AX2bvnjOVD J(G1VGX_u\''_Pug1b?(*ea>h r~py*l'+CW|w.K#Lxpe(kW*E?,=vRNo/^4&M3\6\fCBEH^q*#o?K}s=+`:;Ta?eu|__+2 L)! `;f-z0O.d reZ ZIUm.l]6pD B@k:ssYl74{<&wa\BYtFo 80YpLZt}yoQX6,0cF\$|lrZyhB[E_.;Lw7d R_Lh, (hd r1E#p0 H H  ( ( ( (U ` ` `jj@pnR~LkK46,'M@@9@;!aC1). "-@  6#   # x >%M/.P/ $%(  c18+%:;Q=iJJ lZuD4}vN@`YD*.z7r6H?/.(O6MA#  &1KN = 40 &`9 o x'!9L Bq"5K2XF*Fb'?5+  x<$$DF027uMXoo1?h)d/ppp+4JCZd#p}uhO<D`~0zFp(F)Kmw@*|"nra=^E,"J,R{;x4;fXp:U@:,M@! F,h'Knpp|/52OT{u,sc[JPj$l_W.~i_ Rp>]FX}bhe%uh<x+ FMpyhz-P' ,wV%`ZGZ&pj{pdiC[azdDFiaV.Vd$\Gc]=;[G$" 'AH"G=*8xApL)^_dpKs<bcA'epwX(^9Y.bt0[%bbrD  2pT1)zq#lO`;,hl-J#_-7!Li:BS,#VWo #[~f9*aNbA<TOb JOO"'Q7!M *$ qI+A`OO8, ?}6+!@%.) I 5  o#{BCOTz D}5OQX#K,?4 B>N;>+b"^:`)L Q  *+    $")+)"$# "*$!,/ #  $  '  5("#".1IXW`GAm# CB!0Br!JLx$lq:wnM [N{%o WJ R#m-s8:3);1]DO}3DW$*eQSe9h"Z:|b x;Fowj|w34u6.};"JwZbzBg%?= Dd_,* uq6ugS~2qio >^TR lH  ZU;X s.L;oh%AGATAGAACACCCTCACCACGTCTAACATGCGTTATAGATGGAATAAACACTCCGGCTACCCTACACCATAAAGTAAATATCCACCGAGATTGAAGAACTTAAAGGAATAATTCTTATGATATTGTCGCCGGCGCTCCACACGCGGCCTAATCCTGGAGCGAAGTGTGGACATTTCGATCTTGCGAACAAAAAAGCGCCATACATCACACACTCTAATTTATCTTGGAACTGAACCATTGCGGTCCTCGTGCTCGGACGAGATAATCGAGAGCTCTTCCTCCCAGAATCATATTCAATGACACCGGAGGCCAATCACATTCAAAAAGATAAATCTCCTCTTCATCCTGGCTAGGTCTAAAGGAAAAGTGTGGGCTTTAGGAGCATTCCAACCCAGAAATGAGTACACTACTAGGCTTGGAGAATCACCCTTCTTTTTTCTGTGGTTGCGCAGAGGGGACGTCCAATCCTTGGGGTGGATAGGTAGCGGCACCAAGCCCGATAATCGAAAGGAGCAACAAGCCTGCAGGCCTCAAAGAACTAGGCCCCTGCCCGCGCGAAACTCCTTCGAGATCCTTCACCTCTATCACCAGTTGTATCTCCTCTTCAGAAGCCTTCACCCCATTGGTCCTCCGAGGACCTTCCCGGCGTTCACACCACCGACACTGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:11:02 -04:00AEPt>AEPt>APFN YRAPXV2APrN 5rAPrV1APrX}}5{ARTNASPFASPtASPtB1PtB1PtBCTSBufT OGCMNT 54CTIDO$CTNMO*CTOwO8CTTL 5+CpEPDATA@DATA@DATA@DATA@DATA  %DATA DATA  DATA *DATA ?\Y[DATA ?\طDATA ?\XDATA ?\oDCHTDSamDySN 5 Dye#DyeN5DyeN5DyeN5!DyeN5&DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!5ALNTD2LsrPaMCHNMODF5bMODL3730NAVGNLNE`NOISVOfScL P1AM6qP1RL6kP1WD6vJP2AM6P2BAP2RL6{PBASYPBASWPCONiCPCONfPDMFY;PDMFVPLOC6arPLOC6\<PSZE`PTYPO?PXLBRGNmORMXV1RMdNFRMdV1RMdXGRPrNNRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 4RunN""NS/N%VSCAN@SMED SMLtSMPL YSPACMSPACVSPACMSVER4.0SVER SVER,,4uSatdLScal@Scan@TUBED4Tmpr<UserphARphCHWphDYbigphQLcphTRphTR&This is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN YRAPXV2APrN 5rAPrV1APrX}}5{ARTNASPFASPtASPtB1PtB1PtBCTSBufT OGCMNT 54CTIDO$CTNMO*CTOwO8CTTL 5+CpEPDATA@DATA@DATA@DATA@DATA  %DATA DATA  DATA *DATA ?\Y[DATA ?\طDATA ?\XDATA ?\oDCHTDSamDySN 5 Dye#DyeN5DyeN5DyeN5!DyeN5&DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTabkFVocZFWO_GATCFeat//+GTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!5ALNTD2LsrPaMCHNMODF5bMODL3730NAVGNLNE`NOISVOfScL P1AM6qP1RL6kP1WD6vJP2AM6P2BAP2RL6{PBASYPBASWPCONiCPCONfPDMFY;PDMFVPLOC6arPLOC6\<PSZE`PTYPO?PXLBRGNmORMXV1RMdNFRMdV1RMdXGRPrNNRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 4RunN""NS/N%VSCAN@SMED SMLtSMPL YSPACMSPACVSPACMSVER4.0SVER SVER,,4uSatdLScal@Scan@TUBED4Tmpr<UserphARphCHWphDYbigphQLcphTRphTR|gCzSD*FTN]%jEX@u]YMQ>*N-~VJ96m-=l=fu.bb>/?_>JNo4^uk_9Vu"U9F~A6IxO\JpSbwEiv5Fhr/1r]C$p~/9k]4;N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIOb]rNT]mQ" ~mj*FW1iSx#_1F,|,/x!q9'D!d/swNI|Z{Z{T7M5Ny[.>zeqr[&XTVZd?]wclQ[>A{%i/"E.Om,\eyDW"[F[.e0$^Gj[m]NvJrdytxLl]hl5cS_d.u?zAw3+fF<7:cFU.F4/~L][U_1(_CcS{[}7"=I).zP{?7.N{Cj_"@?j~J!Eb4;,}E -kb+N4+t457fc|+{Wj2oFtX>j3XV]'p8NpdNmbcnM_GZ}ZqQG|urG&/o{o;Mj2Q&?R2gcV]j4 VI6M<7\\rlfF[>_n&*rs4-+=7s%P[jy%7aU+NU[xinJilZbCd]'p8Npdpu#Wc4hAC?-4$&FkNp&iJ-omMOxjYa/k#Pb~;H26t{_"#kI>b0FcS==6X12Dg8YauBQBhwD~EG7&o.janJiRzq`6\qwHN02T(vW3TA=]Ik'2g|ea}wKShKn6g;X6YM%gcmeY_@h.<$.^ka@GpSC>lVm? ##k0$y!TW3nG6`VX+*flVOY(xs/b@j/.cgoC#Q9UP_`G\U5ZfoQe=N^tpu&ofW)NKo-j2Z.bB E:Yh^n@EK5llme`T95P3&5L(g3`zUii5u49_ %EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMKLbsMvF(wD1RuM]Ug+ 4ojwjq>dLz2zvq6;1&t 4e\,BqnMN;[6_b.O_l;{IF}o-,(B~bHjAt3_iP-2ZE<}DU$~~8WYhY8|k~_D}<|hG6`VsIefaqQleA./q}.}:>JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#k@l'Z9d[TCs]jL'#k=sDa)x+7E2]^AEPt>APFN YRAPXV2APrN 5rAPrV1APrX}}5{ARTNASPFASPtASPtAUDT>B1PtB1PtBCTSBufT OGCMNT 54CTIDO$CTNMO*CTOwO8CTTL 5+CpEPDATA@DATA@DATA@DATA@DATA  %DATA DATA  DATA *DATA ?\Y[DATA ?\طDATA ?\XDATA ?\oDCHTDSamDySN 5 Dye#DyeN5DyeN5DyeN5!DyeN5&DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTabkFVocZFWO_GATCFeat//+GTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!5ALNTD2LsrPaMCHNMODF5bMODL3730NAVGNLNE`NOISVOfScL P1AM6qP1RL6kP1WD6vJP2AM6P2BAP2RL6{PBASYPBASWPCONiCPCONfPDMFY;PDMFVPLOC6arPLOC6\<PSZE`PTYPO?PXLBRGNmORMXV1RMdNFRMdV1RMdXGRPrNNRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 4RunN""NS/N%VSCAN@SMED SMLtSMPL YSPACMSPACVSPACMSVER4.0SVER SVER,,4uSatdLScal@Scan@TUBED4Tmpr<UserphARphCHWphDYbigphQLcphTRphTR