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H I J K L U V W X Y Z a b c d e o p q r | } ~  \]^_`abm2<,,,III^^^{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN NNFFFcccyyy   (((00/888???FFFMMMTTT[[_aagggnmmsssyyy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5386a9e7945245d4a078fd5c6e989d75Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-Well(K1316_C9_Lycianthes_stephanocalyx_trnH05KB_3730_POP7_BDTv3.mobSeqPop7zb OhE$ |   D \ Y G 1           $ ; 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M Z c q }   ! , 8 D Q [ e q }   + 6 @ G P _ h t  "-=GR^hy%0=KXis+5@N_lv $4DL[fq} -9DO\k{ )7EUboz'5AK]iy'5@MVdp}&6AMZfs"1;JYhx          """'"+ '"   %%&""                     " &         "                   "                                       """'"+ '"   %%&""                     " &         "                   "                             ?aM=?8bYN+%'MQ?VI%.J <TA>ZY"WK>8BH SEZ!>I_M- AH-U:@ 8- S=4?_'BaV^A.QM$S5A>=DT2 O`A; WX4B D>8@a]-@ 2[N >+M9"aUMEN+M!`"Z+1O32#4P#=:QO_K\*:>U)JI0G7)>% Z'C8 ?3'X@AO13UAQK,!N"3%b!JVG;O3VTD AGOXS6P 9HU06$Q>-d93I() 7^HPZ] 0O3 2OT0b!JQO/FN%\R2U5_$.0= S8c A ?TKa?: ST?L<E!W !"?, .c`*)'^ KOW4J:B? ^@ [C)c a2B: :Y3S1F]U,G&3P(4d,QF25a9`%OB4[7S^IE@"Z$>T(L\Q,&2H8,ES<cZc>^]\l _ q1p1].DO Xe\ @y>F`4UjYgjy<\ ;\mhJZG@p%dYK}'42S@8Tc1,8wLEwI_d ~$dEO-j|RFC`^xbEyR?Hj#Vi[&5Q66 *;V'7K+#/F<kRUe2bI+?&WA 2\[|>!&`=uSk04 dl nt]Poc"yKSNi RA%e i[{.@iJ 0) 1Bs%@3 NcPc| pUB WQRZ'Hd 1 \(5 Hr VK| H H H ( (5 @@@@@x@bxRxx?x;xxxovx=xxxR@489ET*&&S/GF-( >35u9 B>,v$  f   /bD,}N/X/A3z5m6KF4;A)!U 3O-;(94UV%bjE?Z !D  apppBO! phx"*m}XJ+Xa)-(?r'2[wtiT^w5(Psf1;zP>+n{|M? d/jH{4E;v D,!P*Q]])6=|-@wW+3+=L2M9#.8'0_d8 "WD%T1H8TXuQ Ki!+p^@p,KbQ, -x &"kE9e 0)O q >lB 3nk b|~Nr M$2zoy#i)+9?Z /% "N&.?^-orytrz6&5uy0@ U2|[uxzE'DT 8EW)im<[]W5 Bk3DV K[V2>d&x.pGGACCCTCCACACCCAACCTCTAACCTGTGAACAAAACTACATCTGATAGGATGACTATTCTTTTTACGGGGTAGGGGGAGCGTTCGCACCTGAGGGACTAAAACCAAGCATTCGTGCAGGGGGGTTGGTACTATTTCATTGATTCTTTTCCTAGACATTTGGTAATGATAAAGAGGCAGTTGAAATTTTAAGTCAATCTTTTCAAAAAAGTTGAGAATGTTAAAGAATATATAGTAGGGGCGAGTAGACACTACAGAGTCAGGTCGTGTGGCAGAGATCTCCCACTCGCGAGGAGGGGAAAGTCGCCTCTAGTAGATGAGGAATACTGAGATGAATTTTGGTGAGAAGTTCTACATGCTCGTCGAATGAGACGATACGGTGACAAAGGAACACCCACAAGATCTATTCTCTAAGTTTTTTTTTTTTTTTTTCCTTATAGGTTTATGGTCTGTGTTATAAATAAAAAAAGACCCCCACCTACCTTGGTAACGCCGGAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:11:57 -04:00AEPt@AEPt@APFN _CAPXV2APrN ;ZAPrV1APrX}};cARTNASPFASPt#ASPt#B1Pt"B1Pt"BCTS]>BufT U/CMNT ;CTIDU CTNMUCTOwU CTTL ;CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA &DATA 0DATA :u_LDATA :uDATA :uJDATA :uDCHTDSamDySN :Dye#DyeN:DyeN;DyeN; DyeN;DyeWDyeW8DyeWSDyeWgEPVt!4EVNT :EVNT :EVNT:EVNT:FWO_GATCGTyp!!:HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~[LIMS!!;)LNTD2LsrPaMCHNMODF;JMODL3730NAVGNLNE`NOIS6OfScP@ P1AMJ3P1RLFOP1WDNP2AMUP2BAYP2RLQPBAS8PBAS6PCOND]PCONBkPDMF_,PDMF6PLOC>PLOC:PSZE`PTYPU'PXLBRGNmURMXV1RMdNLRMdV1RMdXLRPrNTRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate :RunN""TS/N%6SCAN@SMED SMLtSMPL))_SPACMSPAC6SPACMSVER4.0SVER ]3SVER,,:]SatdP@Scal@Scan@TUBEC9Tmpr<UserphARphCH6phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN _CAPXV2APrN ;ZAPrV1APrX}};cARTNASPFASPt#ASPt#B1Pt"B1Pt"BCTS]>BufT U/CMNT ;CTIDU CTNMUCTOwU CTTL ;CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA &DATA 0DATA :u_LDATA :uDATA :uJDATA :uDCHTDSamDySN :Dye#DyeN:DyeN;DyeN; DyeN;DyeWDyeW8DyeWSDyeWgEPVt!4EVNT :EVNT :EVNT:EVNT:FTabkFVockFWO_GATCFeat..kYGTyp!!:HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~[LIMS!!;)LNTD2LsrPaMCHNMODF;JMODL3730NAVGNLNE`NOIS6OfScP@ P1AMJ3P1RLFOP1WDNP2AMUP2BAYP2RLQPBAS8PBAS6PCOND]PCONBkPDMF_,PDMF6PLOC>PLOC:PSZE`PTYPU'PXLBRGNmURMXV1RMdNLRMdV1RMdXLRPrNTRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate :RunN""TS/N%6SCAN@SMED SMLtSMPL))_SPACMSPAC6SPACMSVER4.0SVER ]3SVER,,:]SatdP@Scal@Scan@TUBEC9Tmpr<UserphARphCH6phDYbigphQLcphTRphTR.iTr8p|r_1{63_`EHsMT@OmAmDe8HR/D2GcA13woF$1PI?p":*Xq)apMf4S><3$G,lwd-l#frL?*Y\FX`@kjJ}B~xGH$i*8j|]]u.ibuILW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A\&C9":?ZEq1hu$#k:X=<;3On&+VN;172e,qz7|!Y<,h`Fzmj7Ul*8ml%R7MK(h_[W!(ZqK(Ic:=Gk;0|5Q1V?|&@8ja:3AGXS<< xrTW?iMAkc PkIp?H^Poa#]}Ko)6V8JX|K2-!;9xxsWD@|DmOY,f *"49J_'JKxwQ4fC\{lKd#8 UC;2i$@jEl0\JWT|+adJsLl&pvedsgbk1^J.(KC4!7`h?&03BSmv^*:U[*WXWo0\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|_&agD~jg6j{th_B$e_4ppOxU&g_n&J;s$dUW@_G0:!y$y3a;/w_z{(J!!PlX.MP?i=qb^Y&t 4e\,B)nEN;[6_b.O_l;{IF}o-,(B~bHjAt3_iP-2ZE<}DU$~~8WYhY8|k~_D}<|hG6`VsIefaqQleA./q}.}:>JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#k@l'Z9d[TCs]jL'#k=sDa)x+7E2]^BufT U/CMNT ;CTIDU CTNMUCTOwU CTTL ;CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA &DATA 0DATA :u_LDATA :uDATA :uJDATA :uDCHTDSamDySN :Dye#DyeN:DyeN;DyeN; DyeN;DyeWDyeW8DyeWSDyeWgEPVt!4EVNT :EVNT :EVNT:EVNT:FTabkFVockFWO_GATCFeat..kYGTyp!!:HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~[LIMS!!;)LNTD2LsrPaMCHNMODF;JMODL3730NAVGNLNE`NOIS6OfScP@ P1AMJ3P1RLFOP1WDNP2AMUP2BAYP2RLQPBAS8PBAS6PCOND]PCONBkPDMF_,PDMF6PLOC>PLOC:PSZE`PTYPU'PXLBRGNmURMXV1RMdNLRMdV1RMdXLRPrNTRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate :RunN""TS/N%6SCAN@SMED SMLtSMPL))_SPACMSPAC6SPACMSVER4.0SVER ]3SVER,,:]SatdP@Scal@Scan@TUBEC9Tmpr<UserphARphCH6phDYbigphQLcphTRphTR