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***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN NNFFFcccyyy   (((00/888???FFFMMMTTT[[_aagggnmmsssyyy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ac567b77af254beea4b98d8763d67517Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-Well K1316_B4_Indeterminate_sp_trnH05KB_3730_POP7_BDTv3.mobSeqPop7z)UyS!>mc4  *^52]X Ltl~e{>   ' [ 'a + 8 4 l..1IF&_ $ 9 P 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R@AHA>OA 3KB.bcpKB_3730_POP7_BDTv3.mobtermTAGGTAGCGCTCATAACTGATGTGCGAATTGGCTCATCCGCCCCCAGCGAGGACTCTTATTTAGATTAATCAATCTATTTCTGACTGTTTTTTCAAGAACATATGTCAGTTAAATACTATGCATTTTCATTATAATGAAAGAAAAAAAAGGAGAAATAAAGCCCCCTTGCTAGACCAAGGGGGGATTTTTTCTACTTTTTTTCAAAAACTCATATACACTAAGAATCGGTCTTATCCATTTGTAGATGGAGCTTCAAGAGCGGCGCAGTCTGTGGGGGAAGGATGAGAATTGTATTTCCACGTAGCGCCATTGCTTCTCAGAAGCTGATTATAGGAAGTTGCGAGCATTACGTTAGTTATCTCACGGACGTTCAAACCTCAAAGACGAATATTTTAACTTAACATTTCCCAGCCAGAGATACGGGGCACATTCTTTTGCTTTCTTGGTGCTAAAAAGCAGGCTGATCTAGATGAGGAGGGGGAGGAAGTAGGACCCCCCAAAAAGGATTATAGGTAGCGCTCATAACTGATGTGCGAATTGGCTCATCCGCCCCCAGCGAGGACTCTTATTTAGATTAATCAATCTATTTCTGACTGTTTTTTCAAGAACATATGTCAGTTAAATACTATGCATTTTCATTATAATGAAAGAAAAAAAAGGAGAAATAAAGCCCCCTTGCTAGACCAAGGGGGGATTTTTTCTACTTTTTTTCAAAAACTCATATACACTAAGAATCGGTCTTATCCATTTGTAGATGGAGCTTCAAGAGCGGCGCAGTCTGTGGGGGAAGGATGAGAATTGTATTTCCACGTAGCGCCATTGCTTCTCAGAAGCTGATTATAGGAAGTTGCGAGCATTACGTTAGTTATCTCACGGACGTTCAAACCTCAAAGACGAATATTTTAACTTAACATTTCCCAGCCAGAGATACGGGGCACATTCTTTTGCTTTCTTGGTGCTAAAAAGCAGGCTGATCTAGATGAGGAGGGGGAGGAAGTAGGACCCCCCAAAAAGGATTA,8IWfu  +5BJTZft !+4>FP^ix #-:CMYeny #1>JVbny&3@KVamw  -8DOZfs $/=HS^iu ,7BMXdq~    & 4 @ M Y e q }    ) 4 @ M X d q ~    $ 0 = J U b m y   , 7 E O [ h t     ( 4 = I W b o { +7DO^ht*8CQ`t+5DN\is )2>JZmv)6CUiv (4AJZbox #4?M\kv)4AP[fr} '1=FUam{#.=GQ^ju (<KY,8IWfu  +5BJTZft !+4>FP^ix #-:CMYeny #1>JVbny&3@KVamw  -8DOZfs $/=HS^iu ,7BMXdq~    & 4 @ M Y e q }    ) 4 @ M X d q ~    $ 0 = J U b m y   , 7 E O [ h t     ( 4 = I W b o { +7DO^ht*8CQ`t+5DN\is )2>JZmv)6CUiv (4AJZbox #4?M\kv)4AP[fr} '1=FUam{#.=GQ^ju (<KY     .#>>"&% '/)/ ;;>'>>>$>;;11>>;;>>>;;>>>>>161>;>>>>>>;3;>;>>>>>>>>;;>>;>>1*>;;;>>;>>>>>;;>;>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>;6>;>6>>;>>>>>>>>>>;6>>>>>>6>;>>>>>>>>>>>>>>>>;>>>>>;>;>>>68>6+1;>>>>>666>6>>>>8>>>>>8>>;2"       "                  $                    !        +      .#>>"&% '/)/ ;;>'>>>$>;;11>>;;>>>;;>>>>>161>;>>>>>>;3;>;>>>>>>>>;;>>;>>1*>;;;>>;>>>>>;;>;>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>;6>;>6>>;>>>>>>>>>>;6>>>>>>6>;>>>>>>>>>>>>>>>>;>>>>>;>;>>>68>6+1;>>>>>666>6>>>>8>>>>>8>>;2"       "                  $                    !        + *>&Ec^,H+CdX-5 ZW5=' \UO@< 3MKK5D>%)/`QXb; QWE,4D XM@(G9W9Y3"MOY6RGF='U9U a 1b\) _K!P+_Y=9OH/8;59O!CB98O <U UFF#C0F=]QHN6-(;X8Q XN \1$#9IV)X;Z[>')*+ LZ<P&))H9+4b:aJ 'JXR 0P2 c02%RB<%#)N>-)A$`PGZ^ L/,^`/' 25J .@/ 2<"<+I[FA5Q_NaKa*2!S*2 >S7#?AFC0D^ EU 3Z_-E0b&X2[N:M._@3S>U,FH92<EbRPI-A"DD X1]`0@VF`.>*:UHIAVb 5`;bFU!^@]1A X?d[K%)(ZGB.) 72TJ *T3N`I#D%'OGFS<+$I:!9@4<2;$ca `aQw xw^ T4  b `M s9:ha_ :`:d1WC n=k"f4"D./:JpC >B[1'gOh6P0T8H:j3qZ  =9Ix{x@R^h<1x\d5:I`k}6L"4=:x|HYQK,VJ,0qtW J *:^M!P-KN(KenrU'td'jz"Z5W D LJ|lF@saPsvH w_t0S$|[^u Y>Qi A1+ y [!bM0Y ~Zt NqDjb SwU~WT^B P4 ` `-@b<4Gre4Y:`[b>|ifuT:b&YE@?/@/T-+ 5.!%#&   } 'V,(F?^|OF p C'.8:[%-64  ,G l 0N`:([2]&1s,:6M!2 IPI5C [O}3r~pTpkrNphp,h,5htOt& pE#S(D8;Zx zQ>kg0G{{f<GE(Bh'.5cZq2(rOtV}CMs'}6qx3lP\NQ$E@/+ $ D|DE49Kl9 !mN]=i~^E$bX}pN'9- i'~\*a`/u32.*hgd rs7Ip w|,L7&|\hL/IO!IUQH+c8=Nb\[ 1+.nX<p i \ u(HHZ D-$*.B<  o 92p}*5f@ $Pm|b8G/R/ M!^+(8wU.Vf3$ O(7!,1+.(+"44>A '!&  !"  &8 #,% +$#=E$#4!##%4  ;7A'"! 1! E4'I%(-S9.1#7"d#7T/#1)M )&M3Acj ?px"2 : 6+Z a-bdV(J#d$F1w, v`" y\S1` |TTN{g"/NlJ"z0a\*_ue?'5-:[Uy!eSP 3_:(d1K) )q`iI =%/K,hcB>_7iAGATGGCGCGCTCATGGGCCCCACGCCCCGTTGAACATACGGGGACAGAGAACACTCATAAATTTAATCCACCCTACAGGGGAGAACAAACCCACCAGGACATATGACAAACGGACTCTGCTCGCACTTGAGAGGCTGGGATTTTTTCTTAGATCTACCCCTTTTTCCTTCCAGAACGTAACAATAGACACTCTTCACCAACTCGGTTTCTCATAGGCTCTGAGTCGCTCTCCCCTTCCACAATACAAAGTTCCTTCAGCAACGTATCAGAAGAACTAGGTTTAATCCGCAACGAACTTGTAATAAAAACGACACCAAACCCCAAATGCGCGGAAGTACCATAGCATCATATGGCACCCCTGATAAACTAAGGTCGGGCTCCTACAATCGGAACAGTTGCGTGCCCCAAATAAACCGTGGGAATCCTCTCCCGGACCTTGAGTGGTTCAAGCCGGGAGCTTGAATATCTTGAAGTTGACCATGCCGGTCGTTTAATGGACCCCGAATGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:11:07 -04:00AEPt?AEPt?APFN WSAPXV2APrN 3rAPrV1APrX}}3{ARTNASPFASPtASPtB1PtB1PtBCTS|jBufT MGCMNT 34CTIDM$CTNMM*CTOwM8CTTL 3+CpEPDATA@DATA@DATA@DATA@DATA  %DATA DATA DATA (DATA ?\W\DATA ?\ָDATA ?\VDATA ?\pDCHTDSamDySN 3 Dye#DyeN3DyeN3DyeN3!DyeN3&DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FWO_GATCGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~zLIMS!!3ALNTD2LsrPaMCHNMODF3bMODL3730NAVGNLNE`NOISTOfScSL P1AMhP1RLdP1WDlP2AMtP2BAxP2RLpPBASWPBASUPCONbPCON`PDMFW<PDMFTPLOC\PLOCYPSZE`PTYPM?PXLBRGNmMRMXV1RMdNDRMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%TSCAN@SMED SMLtSMPL!!WSPACAb5SPACTSPACAb5SVER4.0SVER |_SVER,,2uSatdSLScal@Scan@TUBEB4Tmpr<UserphARphCHUphDYbigphQLcphTRphTR'This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN WSAPXV2APrN 3rAPrV1APrX}}3{ARTNASPFASPtASPtB1PtB1PtBCTS|jBufT MGCMNT 34CTIDM$CTNMM*CTOwM8CTTL 3+CpEPDATA@DATA@DATA@DATA@DATA  %DATA DATA DATA (DATA ?\W\DATA ?\ָDATA ?\VDATA ?\pDCHTDSamDySN 3 Dye#DyeN3DyeN3DyeN3!DyeN3&DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FTabFVocFWO_GATCFeat//GTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~zLIMS!!3ALNTD2LsrPaMCHNMODF3bMODL3730NAVGNLNE`NOISTOfScSL P1AMhP1RLdP1WDlP2AMtP2BAxP2RLpPBASWPBASUPCONbPCON`PDMFW<PDMFTPLOC\PLOCYPSZE`PTYPM?PXLBRGNmMRMXV1RMdNDRMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%TSCAN@SMED SMLtSMPL!!WSPACAb5SPACTSPACAb5SVER4.0SVER |_SVER,,2uSatdSLScal@Scan@TUBEB4Tmpr<UserphARphCHUphDYbigphQLcphTRphTRk]hiqU"T;#6Rl7U?D0^k,h/fP)} ~&]Dn?IO?p[X!m6ul0${N|X-N&W-dOsW&wzl+<,,pCws^D\we*S#ibu@QhCe3fGX.!6k|TIx*&`cz_GbJE6=W!(/tj glOM5cWL N*hbDv.ItJ[DIBG(XX7&EUU=:+i,S#|n[MVCE N|Xe1oH/`Wa?iITwM"f^@/(y8|o5c;9Vge!Yz]/d*sd6S<;1,;P}J&,39:8 !r|XquYFo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEe>2fkNM2y,'~9'+ez^.-QlMaP)BuWZr%"[ T]Wm\y}4r(>8~Vz7}0eq/V-AMV^@5oOC&qq<6(m=':Z^u4|8e7s+]i@CHbB&H+J-]/l!hZ2pE^j?#vu/N'4;M&bX*F8Bhrb8EY|3.B+0T{!Xf] _ocT)FTX>j3XV=K3]1wv;{?kJ:h,r4[-T0M5[S*.J-j~W7.5k|_-S_7}_ooUzbaI23|5njUZ'KD7I 9xxsWD{xvrL>DI6pebvM<$AJT[Mo"$+r:y`Pz;+hj3jT$3o%+0|5Q1V?yRl<`QV-AMV^@c O*Pv>B~:^gWT<;u4#kBE=+Hqd-wvXfT;KBTAq;ah^.p2kSGKI'_2q@,p;YCWeq }X'KtFW.: wT_eD4<0|fP%4X+*flVS:3AGXSXW .C5wz^`]vWs*D_rj|YnG|urG&/o{o;Mj2Q&?R2gcV]j4 VI6MZ4mX+98lXCsgB]vmZ1Mr03}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03S