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NNFFFcccyyy   (((00/888???FFFMMMTTT[[_aagggnmmsssyyy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a983c335ad564960b2cd88469029178eSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-Well"K1316_B2_Valeriana_scandens_trnH05KB_3730_POP7_BDTv3.mobSeqPop7zry7QmV>Q:j$ n 6  u%5Wq^;" 5X{ -mU &~}) O  3 c  Z  baXO % Y /t( Y  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NZ@/?60V0hTmSNT;x:UC6b*mWFV7'k@.FVOTIx:!T@uPtFDpO^u[fxfC:|o)59K3!cU=J./aZ[.;ue`9:#4 yaV%#PtXYz=^Vmf[^I[vR*pY/.yUu@AgN-FK;9 ncAGGAATGGCGCGCTCATGGGGCCCAAGCCCCGTCGAACCTGCGGTCAATAATAATCAAATACTGATCGTGATCGAATACGGATGGGGACCTCTCCACCTGTGGTCCTCACTCGAATCAAACGATTCAGCCTCCGGAGCCGTCGATCGGCACCTCGTTCCTTGCCACCTTGGTTTCGTATTCGCCCATCTGGGGAAGGTCCTGTCTCTCTCTGGTGGTTGGGGCGCCAACGAGACACAAAAAGGCTCACGTTATATGAGGAGAGGGCTCGGACAACAAGAGGGGATGCGCCGGCTCCCCTATGAGAAACATGGAGCACAGATAGAAGGCATTAAGTCACGTAAAAGGGGTCCCCTAAGGTGTAGTTACTGGGAGTAACCAAATTAAAAATTGGGATGGGTGATCATTAACCCTAGTTACGCTTTTAATTGTGATGGCCCCCTTATTGTTTGTTATGGAAGAGGGATTTACCAGGTAGTGGTGTCTGGAGGGAACCGGGGCTGGTCCCTTACCTCTAGGAATCGGGTACTCCCTTAGGGAGTAATTAAAATCTTTCAGTATATAAAGAAGGTTCCGATGCTGCCTATCGTGAAAGGAGGGGATAGTAAGGTGACAGCACATAGAAGCGCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:10:49 -04:00AEPt@AEPt@APFN WUAPXV2APrN 3rAPrV1APrX}}3{ARTNASPFASPtASPtB1PtB1PtBCTSBufT MGCMNT 34CTIDM$CTNMM*CTOwM8CTTL 3+CpEPDATA@DATA@DATA@DATA@DATA  %DATA DATA DATA (DATA ?XW^DATA ?XֶDATA ?XVDATA ?XfDCHTDSamDySN 3 Dye#DyeN3DyeN3DyeN3!DyeN3&DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FWO_GATCGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!3ALNTD2LsrPaMCHNMODF3bMODL3730NAVGNLNE`NOISTOfScSL P1AMtmP1RLthP1WDtriP2AMt|9P2BAtt!P2RLtwQPBASttWmPBASttTPCONttf%PCONttcPDMFW>PDMFTPLOCt^PLOCtYPSZE`PTYPM?PXLBRGNmMRMXV1RMdNDRMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%TSCAN@SMED SMLtSMPL##WSPACAgSSPACTSPACAgSSVER4.0SVER SVER,,2uSatdSLScal@Scan@TUBEB2Tmpr<UserphARphCHTphDYbigphQLcphTRphTR._This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN WUAPXV2APrN 3rAPrV1APrX}}3{ARTNASPFASPtASPtB1PtB1PtBCTSBufT MGCMNT 34CTIDM$CTNMM*CTOwM8CTTL 3+CpEPDATA@DATA@DATA@DATA@DATA  %DATA DATA DATA (DATA ?XW^DATA ?XֶDATA ?XVDATA ?XfDCHTDSamDySN 3 Dye#DyeN3DyeN3DyeN3!DyeN3&DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FTabyFVochFWO_GATCFeat//9GTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!3ALNTD2LsrPaMCHNMODF3bMODL3730NAVGNLNE`NOISTOfScSL P1AMtmP1RLthP1WDtriP2AMt|9P2BAtt!P2RLtwQPBASttWmPBASttTPCONttf%PCONttcPDMFW>PDMFTPLOCt^PLOCtYPSZE`PTYPM?PXLBRGNmMRMXV1RMdNDRMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%TSCAN@SMED SMLtSMPL##WSPACAgSSPACTSPACAgSSVER4.0SVER SVER,,2uSatdSLScal@Scan@TUBEB2Tmpr<UserphARphCHTphDYbigphQLcphTRphTRV"O`x83> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49WW!'YA ruz`Hi4$m=% :6\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvUOT'V4+%UWAG"b>9*O*y~0^O\&t 4e\,BSnHN;[6_b.O_l;{IF}o-,(B~bHjAt3_iP-2ZE<}DU$~~8WYhY8|k~_D}<|hG6`VsIefaqQleA./q}.}:>JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#ky6Ka;v8e7s]jTj.uFn4o[|.]/E7hlC+mNWl26&31x,Af*921kMYYH|O[nY?KPQh^k 1{sI(J8dM!tqw8&rd+'n,J6v$NU,l9cGY@ZWeo)GjL~7L3"6%4y^.N82`\wmj7-U.*P-*KWeq }X'K2MWhc'kT'_C:rF(5,(?z7b[z`>F3!sY#+~/]m#NA^6h9e *E&]E64Ut.K6I TH$^~8[Z*y`89S7wvl_bU=/!F}M`Ws(lu]9g~~hY8|kb+N4+0Oj!Q4M"}JMzR9vAMV^@E P:i~8m+adJsOlG6]}fWG\|J_b.O_l;,[[`7VAd#RBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziP-2ZE<}Q6odPz%X;Frxp/YgnPDMFTPLOCt^PLOCtYPSZE`PTYPM?PXLBRGNmMRMXV1RMdNDRMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%TSCAN@SMED SMLtSMPL##WSPACAgSSPACTSPACAgSSVER4.0SVER SVER,,2uSatdSLScal@Scan@TUBEB2Tmpr<UserphARphCHTphDYbigphQLcphTRphTR