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***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN 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6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 00359194e15a4f1fa915dab6cb124266Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-Well!K1316_B10_Indeterminate_sp_trnH05KB_3730_POP7_BDTv3.mobSeqPop7z)=N[hz@f46 {z ] "  $\O,Xkh5$7<4&$INGK: k X  . 2 a!?_!YtoG*Vq 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J!_/TY;AL3"a-U*}z/ ^ | Qzd$ deEwHdG&0cj2C{5n)(=p'_5LUg[|R;x3$ b=uxvpxg^;NBfC|M*"-P*T/vfv^8 L]7y}I}Bp'TW  !  gq7]G-v n(,H'kRF_]l J$Joij/ /jnAhc]#8Z* Hz#y);zp#Rnhl5sSh-8 yW'W hJXCF'IRQX(e'@cAz/$!m,}H< ZI=( @as,D`0.8=(~#ItOZ.X)sJ+MUWCf(}<FO  HD' ? ` `[/yj1}jNGGl_@N6c\@08'$#7.)3&   .  xxxx xxx        $ !  #  $,;.)#B# ?!A--+?-#:+-6#"  )/D   ]1  @e/_!pV{? wc>p^; d|EIx/$'WCZL,OYb@ !$x]++MFC2usiJ.&*-JIN$svZbpF>`duYdx=q!lPTDN$MA'\CCB:~L57A 9 F;,,lV  Y[p<rC[cX'=edp`4227yG/pz% w9ed&jDInM`Z@UwDCmpN#5mg8D,j6IUR\>,A+J(p,?5D80p t8d"=7e   Za K *$($: qS95k5dMxiT~cRmW]$-pBX +#@!.,,($Gm4\-08D6E5D!(:#("G&!&"!'18][J$.1'%)+& ',-$ $% ##3))"%  (%i!7G\I !"-(+$S",#$$,5A@%;?=O66,.*8+ @!<  L66"**'$% -!-!N1L!%&$+6!"(:G-,U&)3R,$ L !E#- 97 ]P7<G^W5EbZ,A"q)P!F-e,}2>@i#JNGbM88ZS5a|.+|UMA@=E-,5 xlO*ERFM=VP`<9CTC# %4&D<V4m?d80(gG.3(W5C2@Fm]XZ37S/st:YCN,Qm` e<'@PD"AGGGAATGCGCGCCCGTGGGCCCCAACCCAGGTATAACCTTCTGAAACTTTAATACATGCCTAATCAATACACAGGGAGTGAAAAACACGCCCACCTTAAAGAGAATTAAAGGGCGTTGCGGTCGAAGGTAACACCCCAGGGGGCCTAGCGGGGGCTAGGGGGGCCGCGCCGAGCGCACGAGACGCGAAGGGGGAAGGCAGCACCCCGCGACCCCCCGGGCAGGGGGTCGTGCTCCCTGGGTGTACCGGAGGGGAGGGGGGGTGGTCCGGACCCACTGACCTCGTATCCCCTACGTGCACCGGCCGGAGGCATTTCCCATCCCCGTCGGATACAGCGGCCGGGAGCACAGTTTCTTCTTGTAAACCTCCCCTTCCTACCAGAGGGATCCGGGGGCGGTTGGTGGGGACTCTTCATTTTAGCGGGATTTAATAGATGGCTTGAGGATTTTTTTTCCTTGGCGTGTCCCGTAACTCTCCTTGGTAGGTTCTGGGGGAGCTCTAATTGAAACTCTTTAAATATATAAAGATACTTGCGAATCGAGACAGGGTTTAAGGAGAGGTGTGGAGCATAGAAAATTCCGCCAGACAGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:12:00 -04:00AEPt@AEPt@APFN W<APXV2APrN 3ZAPrV1APrX}}3cARTNASPFASPtASPtB1PtB1PtBCTSpBufT M/CMNT 3CTIDM CTNMMCTOwM CTTL 3CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA (DATA =|zWEDATA =|z=DATA =|zM5DATA =|z-DCHTDSamDySN 2Dye#DyeN2DyeN3DyeN3 DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FWO_GATCGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~o=LIMS!!3)LNTD2LsrPaMCHNMODF3JMODL3730NAVGNLNE`NOISC-OfScP@ P1AMOZvP1RLOUP1WDO_P2AMOhPP2BAOOlP2RLOcPBASOOEPBASOOC`PCONOOSPCONOOQ:PDMFW%PDMFCDPLOCOLPLOCOGPSZE`PTYPM'PXLBRGNmMRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%C%SCAN@SMED SMLtSMPL""WSPACAh7SPACC=SPACAh7SVER4.0SVER pSVER,,2]SatdP@Scal@Scan@TUBEB10Tmpr<UserphARphCHC[phDYbigphQLcphTRphTR/ This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN W<APXV2APrN 3ZAPrV1APrX}}3cARTNASPFASPtASPtB1PtB1PtBCTSpBufT M/CMNT 3CTIDM CTNMMCTOwM CTTL 3CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA (DATA =|zWEDATA =|z=DATA =|zM5DATA =|z-DCHTDSamDySN 2Dye#DyeN2DyeN3DyeN3 DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FTab!FVocFWO_GATCFeat//~GTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~o=LIMS!!3)LNTD2LsrPaMCHNMODF3JMODL3730NAVGNLNE`NOISC-OfScP@ P1AMOZvP1RLOUP1WDO_P2AMOhPP2BAOOlP2RLOcPBASOOEPBASOOC`PCONOOSPCONOOQ:PDMFW%PDMFCDPLOCOLPLOCOGPSZE`PTYPM'PXLBRGNmMRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%C%SCAN@SMED SMLtSMPL""WSPACAh7SPACC=SPACAh7SVER4.0SVER pSVER,,2]SatdP@Scal@Scan@TUBEB10Tmpr<UserphARphCHC[phDYbigphQLcphTRphTRFj6%n?oX?HYTw|bT|MI>"oCTRM*?Bcz6F&ZC'nUz}%?^`0L)^,KUS|Xq:u0#|UR7\6|y9o31)-PA)y1XV7:>JMB*u $O6T-jpjK4|<"G+SP7T]Zjp7xMO9j;q6TIUwch.sQ@_=!)}W}oQln:~]a:WkR,uKsH"Kg5!O$AUI/&({T?HK_eXjKy#Id`Po<_+MWQ(SbXW,VE+,[Ol% eIe] `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gXk0[+D:rF(5,fc-,RIlVinJilZbCd]*>ZEx$DB+c7T-O)\rS|5qk`T1jdRC WIFM/|RB"H2gQ.il=O%?2kE]2Z~$dF0Ntm9>|w:=O{h[A>iv<("Hf.QbU`AtG>udrV|gwCiOfF{\)lOYW|_nw|]jTj.uFPqWgZ*me2&dArXx6m}3,~~+N@c'v+gK >o>s1HT+O}L6>U{mwY1XV7:>JW*z(KC,Zy`[qDr_WG\|xT;#6RlSR5[h-er5!_uJUo6X?/WZ#3|5Dfqcd o;X.>S]T'_C:q|4G,fcp,@B_8Es{jGyZ.E/zC&/VnZAEPt@AEPt@APFN W<APXV2APrN 3ZAPrV1APrX}}3cARTNASPFASPtASPtAUDTB1PtB1PtBCTSpBufT M/CMNT 3CTIDM CTNMMCTOwM CTTL 3CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA (DATA =|zWEDATA =|z=DATA =|zM5DATA =|z-DCHTDSamDySN 2Dye#DyeN2DyeN3DyeN3 DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FTab!FVocFWO_GATCFeat//~GTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~o=LIMS!!3)LNTD2LsrPaMCHNMODF3JMODL3730NAVGNLNE`NOISC-OfScP@ P1AMOZvP1RLOUP1WDO_P2AMOhPP2BAOOlP2RLOcPBASOOEPBASOOC`PCONOOSPCONOOQ:PDMFW%PDMFCDPLOCOLPLOCOGPSZE`PTYPM'PXLBRGNmMRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%C%SCAN@SMED SMLtSMPL""WSPACAh7SPACC=SPACAh7SVER4.0SVER pSVER,,2]SatdP@Scal@Scan@TUBEB10Tmpr<UserphARphCHC[phDYbigphQLcphTRphTR