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***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN 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6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 037bb7175b4c400bab77bce2c4e19132Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-Well(K1316_A9_Cucurbita_okeechobeensis_trnH05KB_3730_POP7_BDTv3.mobSeqPop7zhlu2Vz$N{L2k =p5[ygF b)_Y#]1 4Ux8OapzxjV<[.w]D+ 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X+9G2b=$H a_2Oa5XG B 4N({\H'*) :5VTYvi%H'9F3%Y #I%`//VR5 K@[]rUi?.mjpUvQ*^n*(ytyc~B1z"c7qWzAzuCEV XI#lAbky?>J]1AF!l19Gh_ Q<LL9qNqNGr8w`{JrI8K!Lv;`+[ 6A3)-gN:'[a{ |@-_F[R;6$fwo`F) ku8:9{kL3@p< X ju <?Qwi= ??=~r$(,'  s{F3g- $ 8F9\Ps W ` `Y{?ilN+,AjuCV+bUJ5KM8^G_( "dxv$Jp#2!<  C  !/    F , &  J  7 2 `B +4+G"*-NN/ An1g  $      K $ 3 + (    1*1  { ~Lc                     I                             -Spy0pp{nT*pW?k# 8 l8JXA&y_uuy-R k@]IB93J 8H8NmvEn E|_-13o,>D9.?6,818(tEX$FQpC pp1m  'uVXs+0&zpdaBrq C;PThmr&A-FG G_r=8?+ovFAD[rotKC fc15dp(I| V8Djd p,1{lFMu2K0F:Z`;NB Q( ZtF>"}tYF qS2J 548-z,Df~Ct5N^OT}u6ORy*z?JFQI`w{y&HW{x*93yK}^4%(%uQ   uy}/-'_} 9{Cp; +C,pew>1T3f.puwD_Op+;rrqIk$?M6g (?C6Z0C|6C*>a}H7YOPFHzS4:)/'53THF=;1K";"8$5<KO5.;+%$3lU>&=#<LK(/53Bf,(6@+=$  2+35<AT@B\@D=F&A6S;i1V%<+D@Jle'?TaD7S3A@I$o1`P4ARPDOM/{ Fo-QErX~_3\y*("6lG Osh;GGAGCCCGCTCGGAACACCAAGCTACGTGAAATAACGTTTGATTATATTTAAAAAATATCATAAAAGTGTTATTCTACCGTGCTTGGTTATTTCTGGTATAAACCCGTTTACTACTTCTTACCTGCGTGAGGAGAGGGTCATATTAGATCTATATTGCAAATAATCACAAAGCAGAAGAAAATAAAAAATGAAGGGTGACTACTCTACAGGCATGCCCAGAATCCATCCTCTGGAAGTCGAGATATAGAAGGGAGTTCAAACCCAACTCTTAGGGAAGAATACTGAATGAGGATACAGGGGGACGAGAGCGCGTTCTCGCTAGAGCACGAGGAAACAGCGCACTGTTGAAAGGAAAGGGGGGAGGCTATTGTGTTTTTACGGAGGAAAGATTGCCAAATCGGTCGCAGCTTCCGATGCTCCCTATCTTTAAAGGAAGTATTGTTGCTTTCGGTCCGGGCGTAACAAGGAACTGTATTCCAGTGTCATAAAAGGGTATACGTATTTCTTTTTCGCTAGTAGGGGAAAATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:12:01 -04:00AEPt?AEPt?APFN VAPXV2APrN 3 APrV1APrX}}3ARTNASPFASPtASPtB1PtB1PtBCTS}PBufT LCMNT 2CTIDLCTNMLCTOwLCTTL 2CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA eDATA (9DATA ?XVDATA ?XTDATA ?XUDATA ?XDCHTDSamDySN 2Dye#DyeN2DyeN2DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 29EVNT 2EEVNT2QEVNT2dFWO_GATCGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~{LIMS!!2LNTD2LsrPaMCHNMODF2MODL3730NAVGNLNE`NOISTdOfScF P1AM i7P1RL eP1WD mWP2AM uP2BAyP2RL qwPBASVPBASTPCONcPCON`PDMFVPDMFT{PLOC \PLOC XPSZE`PTYPLPXLBRGNmLRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2wRunN""LS/N%T\SCAN@SMED SMLtSMPL))VSPACAdLSPACTtSPACAdLSVER4.0SVER }ESVER,,2 SatdFScal@Scan@TUBEA9Tmpr<UserphARphCHTphDYbigphQLcphTRphTR%ʁThis is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN VAPXV2APrN 3 APrV1APrX}}3ARTNASPFASPtASPtB1PtB1PtBCTS}PBufT LCMNT 2CTIDLCTNMLCTOwLCTTL 2CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA eDATA (9DATA ?XVDATA ?XTDATA ?XUDATA ?XDCHTDSamDySN 2Dye#DyeN2DyeN2DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 29EVNT 2EEVNT2QEVNT2dFTabFVocFWO_GATCFeat//kGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~{LIMS!!2LNTD2LsrPaMCHNMODF2MODL3730NAVGNLNE`NOISTdOfScF P1AM i7P1RL eP1WD mWP2AM uP2BAyP2RL qwPBASVPBASTPCONcPCON`PDMFVPDMFT{PLOC \PLOC XPSZE`PTYPLPXLBRGNmLRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2wRunN""LS/N%T\SCAN@SMED SMLtSMPL))VSPACAdLSPACTtSPACAdLSVER4.0SVER }ESVER,,2 SatdFScal@Scan@TUBEA9Tmpr<UserphARphCHTphDYbigphQLcphTRphTR@~A67OQ1{zK90plkm$48V^p$&:?wMB>-j%;U*E^h9i_Qw|k8 <.(P-S"EnKl/:5t9z"DEoh9ZzZW-W 7W}$wFTN]%jEX@u]YMAmB] ]ddc}PMfOl>o UekB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RUR6? sdWUg>|});C]_nk3RHK2]B(b%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tr%,yPyN+8ds16;evr./b?Ay0]:jjMSDDl[zLTVAjpO-D3TYi~4G$9q8q>(GE[V^rj7}CP~9Dz$j`}c_0f4[42g|5[S*.J-omMOxkYEZ&dAro XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]wnF){xD\4AFU?-4$&#S%TwLhlh9eD>0i#5!yv+R&dT1orml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_|owoqiY.] z,&0@1>X`]yVlX/-f>P5CRV1]cK=i~f&XKEFZw+;;S6FsF#Gy}nLi:B_y&O{1eLt=l{s9;N1jk[K2;+hj3@*7_62Q$OEH5 G,90lVSFsA bFX97z?&q6X+9mK\TI_aEdO{7+VDTj.uFPqWgZ*me2&dAr&;t[W?*b1j glX ,cW`$~+hj:gh_)!hs|O1^M6C,nJ3qo8-@MV^A$+v/y#PzFtSEH$*YYo%@rs{6`Vs=y I/ax]]?EZp9}:>Ju6v;(vJnPkGr#d#"0Koiw87=6"4#O|!fEW<#G@njH[=KubQ2a50jj,4o-8N'nsv&'Y?sxWlZN.YHng=!AEPt?AEPt?APFN VAPXV2APrN 3 APrV1APrX}}3ARTNASPFASPtASPtAUDT~B1PtB1PtBCTS}PBufT LCMNT 2CTIDLCTNMLCTOwLCTTL 2CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA eDATA (9DATA ?XVDATA ?XTDATA ?XUDATA ?XDCHTDSamDySN 2Dye#DyeN2DyeN2DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 29EVNT 2EEVNT2QEVNT2dFTabFVocFWO_GATCFeat//kGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~{LIMS!!2LNTD2LsrPaMCHNMODF2MODL3730NAVGNLNE`NOISTdOfScF P1AM i7P1RL eP1WD mWP2AM uP2BAyP2RL qwPBASVPBASTPCONcPCON`PDMFVPDMFT{PLOC \PLOC XPSZE`PTYPLPXLBRGNmLRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2wRunN""LS/N%T\SCAN@SMED SMLtSMPL))VSPACAdLSPACTtSPACAdLSVER4.0SVER }ESVER,,2 SatdFScal@Scan@TUBEA9Tmpr<UserphARphCHTphDYbigphQLcphTRphTR