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***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN 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6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: dcc6f6b4e62f4bf5b71c906c069eac07Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-Well K1316_A7_Miconia_gracilis_trnH05KB_3730_POP7_BDTv3.mobSeqPop7zjq>[}@Iwf( h x p l f \ N A 9 7 : @ G L N M J E ? : 8 : ? 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J$2mXj+O%   SLI]IcWizA ;8IX+   4oImkBmD@_6:uE#   W kCN$   *]6doSN?Y)_te6k:  .d1W\@ Q+  'al h6    'S%,zJ(  (Z/aug<Q2   '3<@?9/$ 4_a:    *Z58vF% !1?HKG>1 N+^oZ%W+  ^[xu &    "(0;Kc zm_PA4)!!&*.2579;<>?ABCDDDDDEGIJJJJIHHIKNQV[bjs $$|pllopng]PC7.(&&'*.26:>ACEGHJLPTY_dggd^VNGCBDHMQTTPJB8.$  o)4@A7AI(APKB.bcpKB_3730_POP7_BDTv3.mobtermTAGGTCCCAGACCGATCTTCGCTTCTCCGCCCCTCTACTATATTTACATTTTTACATTTAGAAAAGATGAAATTTCGACCATTTATCTTCTTTATTATCTTATTTCTGAGATATAAATATTCAGCTATTCAGCAAAAAATTACAATTTCTTTGTTAATGTAAAATAACACCTTCCTAATCTTTTGTGAAGAAAGTATATGAATTTTATATTAATTCATACAGAATGAAAGCACGCTACTCAATTATGAACCAACCCATAATTATCCTTTTTCTTTTTCTGTAATGTAAAAGAAAAAAAGTATATGTAAGTAACATAATCCTAAATAAAAATAAAGGAGCAATACCGCCCTCTTGATAGAACAAGAAAGAGGTTAATGCTCCTTTATTTTCAATAACTCGTATCCACTAAGACCGGGGTCTTATCCATTTGTAGATGGAGCTTCGATAGCAGCTAGGTCTAGAGGGAAGTTATGAGCATTACGTTATTGCATGAACGAGAGTAGGTCCCAGACCGATCTTCGCTTCTCCGCCCCTCTACTATATTTACATTTTTACATTTAGAAAAGATGAAATTTCGACCATTTATCTTCTTTATTATCTTATTTCTGAGATATAAATATTCAGCTATTCAGCAAAAAATTACAATTTCTTTGTTAATGTAAAATAACACCTTCCTAATCTTTTGTGAAGAAAGTATATGAATTTTATATTAATTCATACAGAATGAAAGCACGCTACTCAATTATGAACCAACCCATAATTATCCTTTTTCTTTTTCTGTAATGTAAAAGAAAAAAAGTATATGTAAGTAACATAATCCTAAATAAAAATAAAGGAGCAATACCGCCCTCTTGATAGAACAAGAAAGAGGTTAATGCTCCTTTATTTTCAATAACTCGTATCCACTAAGACCGGGGTCTTATCCATTTGTAGATGGAGCTTCGATAGCAGCTAGGTCTAGAGGGAAGTTATGAGCATTACGTTATTGCATGAACGAGAG/=PX`kw %+7AMZgt&29FRVbny (3>HRan{ !.:DO[eq}$.9EP\gr'2>IU_kv  *6COZeq|$0<IUanz    ) 7 B N Z g s ~  " / < H T ` m x    * 6 B M Z g r }  " / ; G T ` l x    ) 4 @ K W d p {  ,8EP\gs'3>IUan{ %1=JUbmx +7CNZgr%1=IUan{ "/;GT`lx ,9FQ]jv ,8DP\iu(4?JV^fo|!2/=PX`kw %+7AMZgt&29FRVbny (3>HRan{ !.:DO[eq}$.9EP\gr'2>IU_kv  *6COZeq|$0<IUanz    ) 7 B N Z g s ~  " / < H T ` m x    * 6 B M Z g r }  " / ; G T ` l x    ) 4 @ K W d p {  ,8EP\gs'3>IUan{ %1=JUbmx +7CNZgr%1=IUan{ "/;GT`lx ,9FQ]jv ,8DP\iu(4?JV^fo|!2    4   2 &   2 ".>>>>;>>  +>>1;;86;;>>>>>>;>>;>>>>>>>>>>>>>>6;>>>>>>>>>;;>>6>>>;;;>>>>>>>>>>>;>>>>>>>>>>;>>>>>>>>>>>;>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;;>>;;01";.2;>>;>>1*>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>16>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>;>>68>>6116>>>>>>>>>>>>6>>>>>>>>>66>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>;>>.>;6+>>>>>66>>>>>>>>>>>616>>6>>>>>8;>8>+>;;;8;3  3  )))8+) 3*          4   2 &   2 ".>>>>;>>  +>>1;;86;;>>>>>>;>>;>>>>>>>>>>>>>>6;>>>>>>>>>;;>>6>>>;;;>>>>>>>>>>>;>>>>>>>>>>;>>>>>>>>>>>;>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;;>>;;01";.2;>>;>>1*>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>16>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>;>>68>>6116>>>>>>>>>>>>6>>>>>>>>>66>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>;>>.>;6+>>>>>66>>>>>>>>>>>616>>6>>>>>8;>8>+>;;;8;3  3  )))8+) 3*      $ GM ?+>%N;J\>89'2CYP@E*M :_U410E=`M02 11Z*)W"_2O0OR*"G_F a Lc96=IJa9M.L] DW<O409/#(0 Q%<c0SUM4A-I^AFAPV]2!!'9 ^?Q \" KZ+ cS!ZA#X,+\/:W?$NBVF!UX?#]1.`ZKO?#`7D<H%MG!D+-F6 3RXF+W:(]LE.?Na3XNF= V$Vc`'($#$O11F_(@\F ,_9CU]M$A$D I8V&+3IP@0=& PIO(K@4a?bUZ@b`:1?BM9 = *IHVcZD0GQacX +>*X C QQ +!3KM%/CL,H^P#3< .?7<[1F?/E]?cN`#4IGU/&D]bZ9ZJT)2;@2@ c)*HJEf $ 7piUHK1  |*?8EOk` P>"!oCRkZi@ Y} 7)j zEe3Y.o% x)}C2"  -Lqy_e8lG| )8%B!2. 2:7/5AHua:]UIOQ W&Lx ?67dY c-1}*}Bspi?M <#;G_ y9)Q$(/(Q?!Cwh+D786z8@Gi hU,(lV*H($qIc^zd c1([  3-Mw8eP%o1~b%:X&k`)WA~x;"PG&pIk@`ouU )wno;   H  ( 6 (,*@@4 @o@i@@ah@KM\uxx>>FLxAxBGN: 6!*&  x$         Lx  $ .x7! W%      #n pppppb~ peO]GLfS8?nYu uL2" I9Cg#3x=PIK _2pjp^N=yX W1T )1wK0ApplV=n)P,8%l;{- k }y4Q^Fp4,5rdW1"\GX76x7v+74<0p9Xc/@e/5b)QCn87L;8o4qEuVs #Ttb{P\E>/p@j7L=8P3\C:^ )<; t2ZvqiU )t98*TN%\F1| ?E=P "VQak#+&:O8&S;32+"#'&5"***+!"L$L(0#P*;7'1 & Q-X$'0+$.0W.Z<.(M%'1J4 ##*"'7&.!("#'-/"!#-T4.!Q%")Z@,Q$D<)L < 'G#YM     Y3"X   ,K @"'8.;' %5 )9%"I ;Q#rd%-A '1%(/C$i<IqI085Y/hf"zm;Zko ]?7}4j |tAGATCAAACACGTTCCACGGTTCAAATGCGATTCTCCACTATAAATACAGACATACGGACCGGGGATACGGTAGCACGGACGCCTAGGCGGAATGATGTCGTGACTCAGAGATACGGAGACACATCGGATCTTCGGTTTGCTTCTACCTCAACGCGGGAGCGGCAGCGCGTCCTTCCGCTCGCGCGTGGTGGGAGGGGGTGTCAAGCATAACGCCTCCGTCCGGAAGGGCTCTCTCCTCTCGAATGAGCTGCCTTTCAGTAACCTTCAAACTCAAACTCTGCGGCGGGGGAGGGGGGGTATATCTCGGTCCCTCCCTCTTCCGGACGGGGACGGAAGATCTACTTCTTTCTCCCGAGAGGACGAGGGAGAAAAGGGATCTTCAAGAAAATCGAGGTCTCAGCTACTCGGAGTGCCATCTCAGATTTAAAAAGAGAAATTTACTTTCGATTAGAGCCAGGGTTAAAGGTGAGGTTTGGAGCATACCAGTGCAACCAACAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:11:44 -04:00AEPt?3AEPt?3APFN ]cAPXV2APrN 9APrV1APrX}}9ARTNASPFASPt ASPt B1PtB1PtBCTSBufT SWCMNT 9DCTIDS4CTNMS:CTOwSHCTTL 9;CpEPDATA@DATA@DATA@DATA@DATA 5DATA  DATA $DATA .DATA ?]lDATA ?zDATA ?[DATA ?ږDCHTDSamDySN 9Dye#DyeN9'DyeN9,DyeN91DyeN96DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 8EVNT 8EVNT8EVNT8FWO_GATCGTyp!!8HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~~LIMS!!9QLNTD2LsrPaMCHNMODF9rMODL3730NAVGNLNE`NOISYOfScT P1AMmgP1RLiP1WDqOP2AMyP2BA}P2RLu7PBAS[PBASYPCONgPCONePDMF]LPDMFYPLOCaPLOC]PSZE`PTYPSOPXLBRGNmS,RMXV1RMdNKRMdV1RMdXKRPrNRRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 8RunN""S S/N%YSCAN@SMED SMLtSMPL!!]+SPACAcwSPACYSPACAcwSVER4.0SVER ySVER,,8SatdTScal@Scan@TUBEA7Tmpr<UserphARphCHYphDYbigphQLcphTRphTRJɁThis is the confidence range *ABI_Limits..FVocAEPt?3AEPt?3APFN ]cAPXV2APrN 9APrV1APrX}}9ARTNASPFASPt ASPt B1PtB1PtBCTSBufT SWCMNT 9DCTIDS4CTNMS:CTOwSHCTTL 9;CpEPDATA@DATA@DATA@DATA@DATA 5DATA  DATA $DATA .DATA ?]lDATA ?zDATA ?[DATA ?ږDCHTDSamDySN 9Dye#DyeN9'DyeN9,DyeN91DyeN96DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 8EVNT 8EVNT8EVNT8FTabFVocFWO_GATCFeat//GTyp!!8HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~~LIMS!!9QLNTD2LsrPaMCHNMODF9rMODL3730NAVGNLNE`NOISYOfScT P1AMmgP1RLiP1WDqOP2AMyP2BA}P2RLu7PBAS[PBASYPCONgPCONePDMF]LPDMFYPLOCaPLOC]PSZE`PTYPSOPXLBRGNmS,RMXV1RMdNKRMdV1RMdXKRPrNRRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 8RunN""S S/N%YSCAN@SMED SMLtSMPL!!]+SPACAcwSPACYSPACAcwSVER4.0SVER ySVER,,8SatdTScal@Scan@TUBEA7Tmpr<UserphARphCHYphDYbigphQLcphTRphTR@~A67OQ1{zK90plkm$48V^p$&:?wMB>-j%;U*E^h9i_Qw|k8 <.(P-S"EnKl/:5t9z"DEoh9ZzZW-W 7W}$wFTN]%jEX@u]YMAmB] ]ddc}PMfOl>o UekB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RUR6? sdWUg>|});C]_nk35H~.]8zQ%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tr%,yPyN+8ds16;evr./b?Ay0]:jjMSDDl[zLTVAjqOWW3[E6\3Jb}vQ%j8oTGYjns*A-;Vq&!Z=c':Z^STt4)TK%FyPD[6A|zvn[Y<,h`%mI{Mb=K{_"#kI>b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~yHAj}="WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$X?;9y8hI0.wv|$@^]?\Hif3qE`HoG83:8\_pf|r,!}&A8u|@6veO"F~ONGtx63$;;O$0NON!PBwfmk=;A{%iTK(>V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>