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                                !"#$%&'()*+,-./0CDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmno !"#$%&'()*+,-./0CDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnom2<,,,III^^^{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN NNFFFcccyyy   (((00/888???FFFMMMTTT[[_aagggnmmsssyyy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 607a864b634f489cb83a7ba3e5446a15Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-Well K1316_A2_Vismia_baccifera_trnH05KB_3730_POP7_BDTv3.mobSeqPop7z1^g_SRN #k=0c!7#$&(7)+- .s/012w23L3a3722130.-Z+* (/&2$!0)N c 6 > { y(3-MY  <r 7cn.?0x!QwdXPJD:)kP4!3F[s!MrJ 9 )c$-5!:==<:j8\66n667@7r7k73666|679P; <_<:71*$(2ms-$ D ! 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IIC.PR?\Q07MFQK/!(%#1"` #Q#X;HIF=+E#QR8 KX-M ,NY<-_8ZaO*UY' +C V2BGYV'08 ' 4F;9+<6)V*3#D>\3CTN9>YJK$-;97GX*:`6=8 1?#FYG L.D[-B&U"@#!R9#N- )W?D-#WQF:c"BK-^P'YU[X>*:(PC3-&)AFF Q.-; <K [16A% 89KL#o[Ow!@DTDy$/ h$q Ml6Dtn!SoW7 6M  adSZ G$q >YJ(n #!.`f!u(5c9{#[Z9PmZ@t,$g:(K3O0Q't |m|}yo_AVB65Lfy!W6oU=j^a!yz] >'"(h$Xj4h \M7xM@\' q{CX&0&q -%&MoyF[1f%((Sm Gv f$oh[ r'\Q>LZ0Z^~n  x I$A   3e^ x [x H gP N ` ` `{|dL\?Sp#{ZlWt%TjG& ?G=GRs 4W2>*-  ;,"3gC_ /&  #"8A"&G)EoGy  B" ' Kge0?e:L&:K9"8-15x   ["* ! -5!#/3?*A) @.>$. .< 8!2[    ? @7rW-+9l^7zp%p12{NOV*XsO0?*zR=~l<.gKLMyPA%B});-Mi!"EaQ 6 cQ$;{pn4GCpvWSKBhjYU7L}8h)=2:?hjI2#}7 GK,a)Q*1$ }73;c9$pawhNHcn[[AWsZfN,g.p oPpX=31Sd OUm|v$3xPA{?q:3O*s9edB>D QDip@q0 E2 vd^RaI@BA4B"-[ `$$~%23EPv#>OJJt6Ay4N%il$7F"et$t $}Wsk,0|S;,`~ ^}t&3(eqWmTyxSN*($Rxeb)R7xp@(oS@>rZ<="C')n#51;t7zs("i=GI$+ !.0\fbR_9mB~m7'#' 7:K`'U8Rq  sn$nM].]Vu@ X<%tbV&Q6R](Sv3]t~phs&|N<Yp4?a ;Q WAGGAGGCCCGCCATGGGCCCAACCTCTTAAATTATAAAGAAATAAAGTTATAATAAACAATTGATCTTAAGGCCTAGAGTCACCAAGGAAAAACTTATTAAGTAGTGCAGTTGAAGGCCAAAGACCCGTATAGTGCCTCGTTTTCCTTATCCAACTTTGCTTCTCCCGTGAGGTGGGGGTCTCCTTTGAGGTGTGGTAACTGCTGGGGCTACTTGCCGAGAGGATGACGAAAACGTCTCTCATAAACTGCGCCAATGATTTTACTCCCCCCTTGATTACTCATTGTATTTTGGGAGTGAATTCACCTCCCCCATGATGCTAACTTTCCTTGTCTTCCAGAAGAGGTACTTGGTCCTAATTTCTACGAGACAATGCAGGGGAACCGGTTCGCTATTACGAAGTAGGAATGCAAAATTCGAAAGTTCAGAAATGGCAGGGTGTGGGTCCAGAGTTTAAGACACATCAGTGGTACTCCAACACAGCGCGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:10:51 -04:00AEPt?AEPt?APFN VAPXV2APrN 2APrV1APrX}}2ARTNASPFASPtASPtB1PtB1PtBCTSz BufT LCMNT 2CTIDLCTNMLCTOwLCTTL 2CpEPDATA@DATA@DATA@DATA@DATA  DATA YDATA -DATA (DATA ?ZVDATA ?ZDATA ?ZUpDATA ?ZDCHTDSamDySN 2lDye#DyeN2wDyeN2|DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2 EVNT2EVNT2,FWO_GATCGTyp!!2KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~xLIMS!!2LNTD2LsrPaMCHNMODF2MODL3730NAVGNLNE`NOIST,OfSc? P1AMgoP1RLcP1WDk?P2AMrP2BAvP2RLoPBASVGPBAST_PCONaPCON_PDMFVPDMFTCPLOC[PLOCX/PSZE`PTYPLPXLBRGNmL|RMXV1RMdNDXRMdV1RMdXDhRPrNLJRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2?RunN""LZS/N%T$SCAN@SMED SMLtSMPL!!V{SPACAd{SPACT<SPACAd{SVER4.0SVER zSVER,,1Satd?Scal@Scan@TUBEA2Tmpr<UserphARphCHTZphDYbigphQLcphTRphTRPThis is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN VAPXV2APrN 2APrV1APrX}}2ARTNASPFASPtASPtB1PtB1PtBCTSz BufT LCMNT 2CTIDLCTNMLCTOwLCTTL 2CpEPDATA@DATA@DATA@DATA@DATA  DATA YDATA -DATA (DATA ?ZVDATA ?ZDATA ?ZUpDATA ?ZDCHTDSamDySN 2lDye#DyeN2wDyeN2|DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2 EVNT2EVNT2,FTab|FVockFWO_GATCFeat00;GTyp!!2KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~xLIMS!!2LNTD2LsrPaMCHNMODF2MODL3730NAVGNLNE`NOIST,OfSc? P1AMgoP1RLcP1WDk?P2AMrP2BAvP2RLoPBASVGPBAST_PCONaPCON_PDMFVPDMFTCPLOC[PLOCX/PSZE`PTYPLPXLBRGNmL|RMXV1RMdNDXRMdV1RMdXDhRPrNLJRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2?RunN""LZS/N%T$SCAN@SMED SMLtSMPL!!V{SPACAd{SPACT<SPACAd{SVER4.0SVER zSVER,,1Satd?Scal@Scan@TUBEA2Tmpr<UserphARphCHTZphDYbigphQLcphTRphTRK2OI*-V>yj6m}:)9`[?y[nvbeK]TM7l:2Fj62o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sbV_f7o,f9r;qBwJUo6;^UWZi7WeOzJu%~4%rIZHp#)+KT>:L-Ev!$V@AKM\8}48vc. 7$?Z2o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*M0JNbm|XMrDK"*.GHiJ(/\_'MChMoG;KMJ95nM,aVh6dYHp-Op;ZJLgS]jTj.uFk4W7|!y]18`'|_n?26.?bFWJQ:Ocl2[ALYZB TTe::AkR4"i`gqFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72We|w:=O[;HA>iv<("Hf.QbU`AtG>udrV|gwCiOfF{\)lOYW|_nw|]jTj.uFPqWgZ*me2&dArXx6m}3,~~+N@c'v+gK >o>s1HT+O}L6>U{mwY1XV7:>JW*z(KC,Zy`[qDr_WG\|xT;#6RlSR5[h-er5!_uJUo6X?/WZ#3|5Dfqcd o;X.>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_^Z0b>"EJX.=DaJ2`>nX$t!TQqvLWvO$iEZ#6)Q>[!5bp~ofpPwmdD9>`MuO\LKZI\T(k"T'o)6yHAj}=