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"*3 !     %+;9K[STgNQ2'/ %    -'8WVYP[WxbqMbfTwbyzt[dI3'!$**,!  -)'L7KRRJ@0%'%"%5%"$   DDSb  yaM/-9 & 9IW|liaT8%##  %& 853@[fdtcNA08%%%!(/ #B4W^](H_OjvYV)Ce|y}V*$!31"3O3+$/vrvcfdlev>gQdv1!uys{Z>.xpth^rt!:zl yfe?"5""& bMNOPQRSTUVWXYZ[\]^yz{|}~MNOPQRSTUVWXYZ[\]^yz{|}~m2<,,,III^^^{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN NNFFFcccyyy   (((00/888???FFFMMMTTT[[_aagggnmmsssyyy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 35ad5bdf5e9d4a1ca48b67852e58c278Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-WellK1316_A11_Ocotea_sp_trnH05KB_3730_POP7_BDTv3.mobSeqPop7z !$)-27=CJRZdoz.DZp /=IS]ekopmf[M=+t[D/  '.25652-&ujaZUQNLKJKMORTWYZZZZYYXVSOJFA<72-)%"!#%&'*09FUfx2~0YbS9 "5FK: fTxoe\TLF@;730-+)(&%$$$%'*-159>CJQX_ekquz~xqjfhquV<,%%*/34310..-,+)'$#!                         &.5;>?<6.%  !#&),0258;>AEHLORUWY[]_bdfhiiihggfecb`^\ZWTPMIEA=:63/+'#     Bs  nA  !#',047;?DJPUY\`dhnt{#5EQY]ZQB-tmmu M />=0    1Tpxd2Bl[L>3+%!     $+27::84.(!        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"T  K+Kc$cI%*>3*EVURXHE)dX WZ'>I5 D=A:_?T67L8cbbW= <(\F@6K';N=9X("3G(B@`DQ4Yc=!5 "=^!YTL RN <YBAM^Z 1!/L\QW\`3? \O^*SSOY1K6Z4 :P I*W13=/ADJ]B[!L D :$^M =/T8U.C9"7b6FR3?$B V4F ^PK/,+aOB!F0PA2#C5[-62(4<FR8#:T6B c"?" X 4-a dRXb?]H_UQ(VE G 8,Y\=80 ;<^K<.V` I,I_ 8% "]49=/ X#c #9T`/"DLL02'HPC2Y2NO_?%a%/$G'a9Q-J]` [N =0/%/2? 0] ?_$7-E>0 g GBx D@_A '%uN n[~VwDD,GB-J>n?BQ~z-,6 WM275]ca>R#@Jq.is-@Rmq)h G@WknfQJuG (  ,v P=2#6uq|+)HP69 \J j "cP"dO{$7|K5 Xn\F@ Yl's<,45 s.8o KJ] 3d3'QzSP^e&q>]:vFtjF+@pb{ e*iR#h!B! Ea*Xg$m`6]9*W8d LGJkl[OebJV`t/9N h b0  ]K;(#w `"fG82i`Y4u. 1&72+@=OZb ?9=h6:7GM  J c,p(1 S qs +ni& ?t L :v P  tm?a ,`ep ` ( `}DeGu}\ Edly;6J}3W"aBeP1JhK  ,.,`* <89RE$c8-M'Q +  #!/) 9> # e   ) K ! C0  &A/$42 )0rY'! :88/, %;/(`%V/->)R!+!|4 #%@70!=% (0=$ &17+?6 %G185=7-<%B4'o U"3AAA?K,77777=wHGG77V+VGS'G*GGAG?77dA\i6Cb]tYRbyQXQ.iiB_ZK@VY^ccRii6iicOi\cikkiciciiiiccxciiVici7qOOckiOOkcOkcikOcckkccdpI8p:'p imp)^dp8Ge_!%d7tEJ<"(jmHNS'p2D %&__nWMNFDM!7,pXP!|]J" Z Rd`<m3ve(CTj8zZ[MqZz(kSLFCQ;pdcl `I4T&3CX2dm,oK8wAR*^L9g,,C/Bc@%HNG,VDGnv8(%A,]AQ8FF7ZP=D2jFp,d)ASd)#a3}8FrDv- ["dD.Q *,*8p}!'=8R,j8dpxiJ>pp1,q+W>"DK4\K[y".$jI]1  Z&L 'I [>dI&? /lAUq"MlE/jUI>0mViL%i3Q)\ 6Zlr2^g3Av\|}{(y1w%4^AfHNj  N~9L+eQ8hx!m-dp[s(i 3ci{ = SY0*@f+,U>7?{.d3aN axphb\7>k_Uaw_`V~~hp20V0^j[i 9Un$9>Z}Ixq3-GUC`8Wsqn<<X*] cpYT@iP27U1.^#[2HeH]^u6*~<LTNkFENMeZUZRYyPe4'_L7N2GZMoZ`.nE3|752Z6SdEzx?99GHjgC*yCdiOy/@J2'CBI+*'mytu8AGAGCGCGGCCCAGAGACCCTAAACACATTAACCGGACCCAAAAATGGCATCTATTGGTTAATCCCCTCGAACAAAGACGCACCATACCCCTATAACTCCGAATATCTTGAGTAAGATGGACTGGGAAATTTTATATTCTGGCCCTACCTCCGGTCCTTTTTGTTCCCCGTGGGCTCTTGTATCACGTGCTCAGTTGTGGCGCATTCCCCTAGAAAGTATTAGCTTAACATGCGTCCACTGTGAATCTTTGAGCTAGCAGAGTCATCGGTTGGACTCACGTACTCGCATTGATGGAACGATACATAGGGCATGGGACTATACGCGCTCAATGAATATTGCAGGGTTTTGTGGACTTGCCCCTATACTTGATGGCCCGCAAGACCTTGGCGACAAAGACTACTCGCTTCCTCTCTGGAAGTTTCGACATAATCTAGTTAATATTATTTATAGTCGAAAGGACATTCGTAGGGGCCGGTAGAGCGGGGGTGGGGGCGGTTTAGACCCCCCCCTGGGGGGGAGGGAGGTCTTAGTTACTATCTCGACAAGAAGGATAGGGACCGTAAACCATGCTCAAAGGGAGGGAAGGAGAAATGAGAGATATACACGTGTAGGCTGAGGATGGTTCGACGCAAGACAGTGGAGAGAATATTATTCGTGACGGAATTTGGATAGCCACCGAAAAGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:12:16 -04:00AEPt@AEPt@APFN VAPXV2APrN 2APrV1APrX}}2ARTNASPFASPtASPtB1PtB1PtBCTSbDBufT LCMNT 2CTIDLtCTNMLzCTOwLCTTL 2{CpEPDATA@DATA@DATA@DATA@DATA  uDATA IDATA DATA 'DATA :uVDATA :ubDATA :uBDATA :uDCHTDSamDySN 2\Dye#DyeN2gDyeN2lDyeN2qDyeN2vDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT2 EVNT2FWO_GATCGTyp!!2;HCFGCEHCFGHCFGHCFG==InScInVtLANE_LAST~~`LIMS!!2LNTD2LsrPaMCHNMODF2MODL3730NAVGNLNE`NOIS-OfSc= P1AM\HP1RL\CAP1WD\MP2AM\XP2BA^ P2RL\SUPBAS0PBAS-PCON@PCON=PDMFVPDMF-PLOC\8PLOC\3-PSZE`PTYPLPXLBRGNmLlRMXV1RMdNDHRMdV1RMdXDXRPrNL:RPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2/RunN""LJS/N%-SCAN@SMED SMLtSMPLVkSPACAjgSPAC-SPACAjgSVER4.0SVER b9SVER,,1Satd=Scal@Scan@TUBEA11Tmpr<UserphARphCH-phDYbigphQLcphTRphTR=qThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN VAPXV2APrN 2APrV1APrX}}2ARTNASPFASPtASPtB1PtB1PtBCTSbDBufT LCMNT 2CTIDLtCTNMLzCTOwLCTTL 2{CpEPDATA@DATA@DATA@DATA@DATA  uDATA IDATA DATA 'DATA :uVDATA :ubDATA :uBDATA :uDCHTDSamDySN 2\Dye#DyeN2gDyeN2lDyeN2qDyeN2vDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT2 EVNT2FTabpFVocpFWO_GATCFeat//p_GTyp!!2;HCFGCEHCFGHCFGHCFG==InScInVtLANE_LAST~~`LIMS!!2LNTD2LsrPaMCHNMODF2MODL3730NAVGNLNE`NOIS-OfSc= P1AM\HP1RL\CAP1WD\MP2AM\XP2BA^ P2RL\SUPBAS0PBAS-PCON@PCON=PDMFVPDMF-PLOC\8PLOC\3-PSZE`PTYPLPXLBRGNmLlRMXV1RMdNDHRMdV1RMdXDXRPrNL:RPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2/RunN""LJS/N%-SCAN@SMED SMLtSMPLVkSPACAjgSPAC-SPACAjgSVER4.0SVER b9SVER,,1Satd=Scal@Scan@TUBEA11Tmpr<UserphARphCH-phDYbigphQLcphTRphTRFB}}Cg/&D&Wr`s;bqz^]/r$^.>6pe:*>^Jp@48Pl: &Pwi~69b[dWUlh{.4eY\`;w>xPqL6`nCRNX}a/JBcw*jEth@t#Rt+7+:}JcLL-GIA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]kId`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{c|5<&n5N`!1;U*W]S#|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTve!@;Ps>4]F%65r#W3}hxv_1=|8Ifz2wHiD(g=0ia}yI?t_tG|T`S'_[PTcM#yKAu22`ArIxT6e&?bFW3|>oW~!Te4;,: TVc:q?khbmV\vx+M?Axb0p$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VL&p?)_QQe|vH[p!hjqf[MH-YtDSYcJ|_nO*rSW,0cM&rAmdF7#WSW3Uny]/\U:"iPS!T/RD8z{&]t[XSXW .CANq~Q9U,Z:X=<;'4"C\|J_@A[]"TM,s"6rg8Z~9}__';_/x"ieGlhx6m}PzN?29zxhW)!hs|*>H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,I~<'",s@3[{R9Ya)@Xp}"aFV;LX4KU>?+Nhsr5%NH!V$tUS+[-GT[y]r.*V2J?'[nVr]_y!iU5|8e7sINi@C,_,qz7z}yaih?]R_s_bed{a9_ajFO-U[dUDNxGq@p'Ic62nrD5pY^d,/IQ}{sGitG3(|}jGPNYQIxw}H{-*<^k8e7?GOV5ro{`qafE*&aF2`_c&a6';_szT/"a/hV6T]==6X*s$[-T_sKQm5APYXA\95Lq>l>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.