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DHHHA@@ZZcpo~   !!'((///666===CCCIJIPPOVVU[[[aaaggglllqqqwwv{{{~~~~~~}~}~~}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~~}}}~}}}}~}~~~}}}~}}}}}~~}}}}}}}~~}}~~}~~~~~~~}~~~~~~~~}~~}~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ace57fbd5431488c8afdeb0e5bcd5566Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-03_23-20_01463130Seq1285412854_Steier_K1322_trnHjmadera96-WellK1322_E10_trnHKB_3730_POP7_BDTv3.mobSeqPop7zX^m6Nc6cWM<\t}nY><^pF lL4$!'+/39EXs !1=CDB<60*"{iYH2ubRHEEED@;;?JXhv -9BKSZ`dfea\VQOQZk#iKEFoaQ>%{[=! 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(?c31fB)   !4V;^dMjE0(%#   2R"iK<:?EHF?6-$)6CLQQMG@<=H`8l{NrR=1+((()*****)(&"$4G\mwxpcXXk=-;.!{j^TJA7-%"$%#          @rAPAU@A˒KB.bcpKB_3730_POP7_BDTv3.mobtermGAGGAGGCCGCGAGCAAACATGTCTGATTGGCTACATCCGCCCCTAATACTCTATTTACAAATTAACAAGATTCGAATTTCTAACATTTACCATTTAATTTAACCATAAGTCTTATAAGAAAGAAATATAAAAGTAAAAAAATTATTTAAGGAGCAATGCCAACCTTCTTGATAGAACAAGAAATTGGCGATTGCTCCTTTTTTTTTTCAAAAGCTCGTACACACTAAGACAAAAGTCTTATCCATTTGTAGATGGAACTTCGACAGCAGCTAGGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAAACGAGAGAGGAGGCCGCGAGCAAACATGTCTGATTGGCTACATCCGCCCCTAATACTCTATTTACAAATTAACAAGATTCGAATTTCTAACATTTACCATTTAATTTAACCATAAGTCTTATAAGAAAGAAATATAAAAGTAAAAAAATTATTTAAGGAGCAATGCCAACCTTCTTGATAGAACAAGAAATTGGCGATTGCTCCTTTTTTTTTTCAAAAGCTCGTACACACTAAGACAAAAGTCTTATCCATTTGTAGATGGAACTTCGACAGCAGCTAGGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAAACGAGA"&OU[in05BVgx (7@HS\eq}  (3ANXdp}'2>GTanx'3?JU`lx %0;FR]it%1<HT`kw +6AM[fr|    ( 4 A M Y e q |   + 6 A M X f q }  $ 0 < G S ` l y   . 8 C O \ h s    * 6 A L X c o }  $0<HT_lv"&OU[in05BVgx (7@HS\eq}  (3ANXdp}'2>GTanx'3?JU`lx %0;FR]it%1<HT`kw +6AM[fr|    ( 4 A M Y e q |   + 6 A M X f q }  $ 0 < G S ` l y   . 8 C O \ h s    * 6 A L X c o }  $0<HT_lv   " *%(%/. $ *40'>8! 88'*8! />/!883>>3>88,+8#,3;,2;,.;1.8;+1#>;3/+.22;>>;;>;;>8>;;.;328;38;288;;;883;;;>>;>;>>;>>;;8;>>>;3;;>;>>;;>;;;;>>;>>>>>;;>>>;;>;;>;>;;8>>>>>>>>>++-3-+%2&& 2&*>8>/88*638>>3*3882>388#88>8'$838>>83888*/8388 '83'888&))+$/8>>88/6>&(8'/++046+/2.&/4    " *%(%/. $ *40'>8! 88'*8! />/!883>>3>88,+8#,3;,2;,.;1.8;+1#>;3/+.22;>>;;>;;>8>;;.;328;38;288;;;883;;;>>;>;>>;>>;;8;>>>;3;;>;>>;;>;;;;>>;>>>>>;;>>>;;>;;>;>;;8>>>>>>>>>++-3-+%2&& 2&*>8>/88*638>>3*3882>388#88>8'$838>>83888*/8388 '83'888&))+$/8>>88/6>&(8'/++046+/2.&/4 ](6.B*OC,ZG`VX%-2IEVW !F%B4MU TZ<SZ 3OF E1/5]#2cV($":[4PW-.Y A::1@64H:_QYa"$ =J%' ?.b/Q B\5!% $/9,$< bH;H@'&8aCW&F%M OJ6F+A* 747,RY6L LY-F 6$M?2.a0B>'K79H27(%3I1 %98Q%!R'4G N5[/M207'1`''0TOc CI.<M7(!5-<!>+(Y1>-%NKa,YJ"#cB%4Eb#NR*KC20'WV /ODgx.u*zqVzsGKIpcI  =_8qu6';:_\Ne+r=%N^*83 zuYD;QXd\\eO8L@_G@Yq:*O:y-| _rl8o,G=umC2[5@V2@' _wYd{JXO@^AQT l9~4 [T Hp6X~ j,6}63}i,I9cPTLMVyRu?9a?mP'F)'2AP$,#-+9$&**.*$'  !       $     3! 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(G 18&!(-%'))&#5   :O =' pddp@phpE'd#N~7C1[qM 5FR,iKE;k3y1dB8pV.Axpk*q+,Kznup%T_d8NlMZn,VI] {f5~z83Db 3F6gIVs P6;3,hVCI|y?]0v<U[[0P=P`RG:8[&(R&3]Ec`K.90B%1%*us8(er<RA=3R\BckGQ8zM='+("GAizN9Al_*d94G:<-9>TS|8p=Q0^B%P;7%,,>3C9Q2/JyBg9BcZ ?R#FRY<1<YU}RGf3`2g]Ab=6M]Y 3urv"[9MI_/[?DrYO`p?ZH|u8PT?A)N<f29$!Rb@urWqh`??]u)qX?MAGATGCCGACTTGAGGGGGCCAAAGACCGTCGACACCTGCGAAAATTATACTATAAATACGTAATCACGTTAGTCTTAGCGAGCACGACCTACCGATGAAATCAGGGTGCCTCAGCCGTTTTCTTGATCTCTCTGGGGGGTTGGCAGAGCTCCCTCGGTGACTTTCCTACATATATCTCTCTTTCGTAGAGGACTCATGGAAAAAAACACTGGTTCGTGTACACTATTAGACTCCTGGCATATTTGGGAAGAGGTAGTAACGCGAGCACCTCGCCCTATAGCTAGGGGATGAGAATAGTGCACACGCGCAGCCAAGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-04 00:51:27 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS1BufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA &|LDATA &|L1DATA &|L~DATA &|L˄DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~0QLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM>|%#P1RL>|"P1WD>|'P2AM>|,P2BA>>/P2RL>|*PBAS>>PBAS>>PCON>>!iPCON>> +PDMF|PDMFPLOC>|PLOC>|3PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 2RUNT )RUNT 0Rate RunN""S/N%|SCAN3SMED SMLtSMPLmSPACMSPACSPACMSVER4.0SVER 1SVER,,nScal@Scan3TUBEE10Tmpr<UserphARphCHphDYbigphQLcphTRphTR$8This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS1BufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA &|LDATA &|L1DATA &|L~DATA &|L˄DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab@5FVoc@$FWO_GATCFeat//?GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~0QLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM>|%#P1RL>|"P1WD>|'P2AM>|,P2BA>>/P2RL>|*PBAS>>PBAS>>PCON>>!iPCON>> +PDMF|PDMFPLOC>|PLOC>|3PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 2RUNT )RUNT 0Rate RunN""S/N%|SCAN3SMED SMLtSMPLmSPACMSPACSPACMSVER4.0SVER 1SVER,,nScal@Scan3TUBEE10Tmpr<UserphARphCHphDYbigphQLcphTRphTRS1P7!3A2Qi4'.QmjMy J1i2boR"^OH1sbu!M4M\mk*+_Wr9|>u2KTTkw^8-l278;;9Vge!Yz]%MJeVId4|Cn=aib*y%`;5m;*/P9m4%2pQWi?oFciW 45=)ySQbir8r|:7'I3#wor({Y]?\/t]66{qT[sEAhiW?WUBs*u hb7+#?Q{6Jn`|T6{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+rrbNn%Q8^L."gE{}Vm)HNtXehapc?_)54n,\(nqBz_^_<~H)&6AEMCBt9}j~:Hz4W9W3|7nM6!"/D?*128)TK72K`rx./vtFl "K_*]$lo#zM!ft,+!'[/ C,Z:X=<;`O|g+Vsp@OJ-12Mm8joIiD60mPa6(U.*)./"Dlyj,K]yDWa%2gP')3g;Z6ObeIXz7_lnqR03AGGK.97V=y`6@vdeW\jhAZG|JWm;2FVOR(_Y~8b`KJk/&UPh_^_,AG)!OiK(/(WWh[8:h%ZFnlk)cP\}:miPS!T/RD8z{&]t[XSXW .CANq~Q9U,Z:X=<;'4"C\|J_@A[]"TM,s"6rg8Z~9}__';_/x"ieGlhx6m}PzN?29zxhW)!hs|*>H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,I~<'",s@3[{R9Ya)@Xp}"aFV;LX4KU>?+Nhsr5%NHxh$jUK+{GaN!dMn,? 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