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z x i d h g c u l l r k j s u >Jio`; U ] W ] O R Z 6 I X J Y w d [ m Z g n ` Y [ ^ N W \ a V ` a Z u v Y z b { r t w .MlW; o d w \ ` ^ @ H V 4 H 6 P @ K = m I h B W W ; a T V a O d Q t r z } k ^ h } t v h k ~ } q c \ g c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
9 2 J = W , ) ; F + , ) 5 = - > . = @ 4 A 8 4 % 8 ) T D , 6 G 5 " 8 1 / 5 > + ? ? : 8 J * 3 5 A 5 5 - 0 F F B ! I 1 - 1 , ! A 7 1 4 ' - ! * A C 9 " < T < 8 4 E E K < ? " " ; & E 3 ) * 2 = 0 2 0 ? ( # D 9 I 4 $ < 1 - ' 8 = C 8 ; H : 6 2 D 4 : = E = 3 8 , 0 % % 4 5 9 1 5 * - & ) : ? 0 < 0 A " ! : + 2 2 4 1 ( % 0 1 7 ; 8 ; , M A @ 7 4 7 , ; 2 ) > > 6 H < 4 8 , = + Q 1 1 4 3 # ( , = 8 & 9 5 2 ; 7 4 < 0 = " . - ' 1 * K ) < ) B F 7 G & 8 / ? : ( ) 4 + ? < L " % 5 0 9 , + 9 + E 0 H $ 4 2 - > ' 5 2 ' * ' 4 0 5 . * > Q B = 3 , B % A - ) D ; 1 3 6 ' B ? F 4 ) 8 . % 5 C @ E ( U J 3 ) < % : 1 0 + 6 ' 7 7 8 @ 5 7 6 @ A ! @ @ W # @ 5 5 & 7 / 6 H 1 D M 2 5 > ; > 8 * ; # 6 A @ @ . , 2 , . 8 6 ) : 7 / - ? 9 ? G 5 7 : ? < A > ; 7 ) = 3 % 4 ! . 5 / " " < / 9 @ K 5 * : H 0 A 6 A 7 = 4 B : 3 E G I ( 1 3 , ' 6 4 ! F [ 0 D 2 7 $ 6 2 : 9 4 @ * H " : 5 ) 1 ) 1 1 ( + / D ? 9 3 / 8 3 4 + 5 4 6 E > < ! 8 = : , R - ' / R 9 J , 6 : * 3 0 3 9 ) 8 = B - ? 9 * / $ $ ) ) B * 0 D 5 % + = % < 9 9 2 E 7 6 H & < 2 + E . : > 0 5 # , 7 ) L : ' 9 < 9 & : A O 2 # / - 0 > + + 2 9 ; ' ( I 9 < 1 , : 4 0 0 2 5 4 = ; 0 ; 5 % = 2 < 4 ! ? 2 6 4 3 % 6 ; 0 3 , - A / C < ) 5 5 $ A 2 8 , " 8 4 = + 9 + 4 7 6 4 > 6 " L 4 ; ) ' ) 1 > 6 6 / " 3 3 - ( B * 2 5 3 6 ) I # 4 * ( 5 * 3 2 . * A ; $ # * / 1 A < - ; 5 = / ! . > % + 2 + 5 9 A * / ) + 8 * J 7 + 9 $ 7 2 0 ; 9 = , 9 = J 8 6 5 0 # / @ $ 1 # ; 0 2 @ / 7 ; 7 8 7 8 & : F & 2 5 - = ' 1 5 < 9 ' * 3 $ 3 7 5 @ - 7 - 4 $ * 3 3 Q . 9 - < . 9 > : ; ; ? < 6 2 * ; , 2 ) ; 0 & > A 3 > 5 0 2 7 & 5 E : 1 " 0 @ A . 8 : 2 9 1 / 5 1 7 , ) = B - G L - 1 5 : 4 . 4 B & ; ' 9 6 ) 2 * - + 1 2 ' / < 5 - 1 & 3 2 6 T C / $ 0 9 R < H , 3 " D % 7 " - 8 7 3 , 5 > / / I 6 3 8 F 3 2 ( % ' 7 ' 6 / , 8 A : / 3 ( 2 ; 9 . 0 ( : U 9 0 , 4 4 6 , 5 B 1 # B 9 8 ; - D 2 8 & - . & 7 $ / ' 1 ) 6 ; A " 5 E + ( - 9 ) 1 < @ K 6 8 = 4 2 4 0 . $ 0 8 + 2 ' 7 G 3 ? ! - N 2 % E ' C = - C ; E ; C ( " , 3 7 - B 4 G % 8 6 ) 7 : , 2 D $ 0 / / ? * 1 - : 6 ; 1 1 1 5 1 / 9 ' < : 8 0 5 B B 9 > . A 1 ? ? 6 3 1 = 5 ) # - > : 4 B / < , / 2 1 3 ' 1 4 9 6 ' E , 7 5 > 2 + * * % * ; 6 6 9 3 ' 1 ' > B ' 7 = 6 % 5 7 0 / , 6 2 = ( 7 & = " F - 8 4 3 C 0 ? ' ! ( & - A * + 9 = 8 7 : , 7 , F 9 9 < 0 * $ 6 7 0 - 7 ) A ) ? 0 5 + 7 1 D / % ; " 1 C 2 : . . ? / 9 & / 6 1 " - $ 1 K ' 0 ' 6 3 , - & & 3 6 : 1 4 4 > 2 3 0 4 7 ! C N < , $ G 1 5 6 2 4 , 4 2 G 0 # , ; 6 B : ' , 7 3 ( , , 7 : = # 4 + 6 ! 0 5 . 7 ) 5 & + 5 ) 8 9 > 8 2 % 6 3 3 3 ) E : E + 7 1 < B # ! A B . E ; 7 0 / - : ( 9 8 6 ) $ 4 / @ A : / . ; & # / 8 7 . 0 A 8 < ( / ) F / . D / 5 $ ; H 4 3 A 6 6 ; ( 6 1 . F & 3 1 E 2 2 , 1 ( 1 7 @ < A ( C + B 9 8 $ ? ; 8 H ; - # 9 0 ; C , : ) 6 : & 1 G $ ( 0 + : 1 5 - 8 & 2 C , 7 ) , - 5 & = . 9 > 3 6 % 0 + * " 7 ) ) 9 D @ < : : 9 1 ? 5 8 1 C * 9 % * B : ; / @ / 4 & / 6 ' ) 2 - 7 - / * : 3 . % 8 & 5 " $ 4 B / 7 ; 5 ; ) - @ 6 8 ; 1 * . 1 3 3 5 < ; 0 , ) 4 6 : ? - ' 0 ) ! 9 4 C 5 0 ; 5 0 . 8 0 6 9 7 ? 2 7 , . 5 6 . A 1 6 : ! 7 % * ' ( = " 2 , % 0 1 9 5 , 1 - / 8 9 < ( 0 8 * - 1 5 . J ' 1 0 , 3 / 1 , - > = + @ ' D - < ; $ " 9 ' 8 2 ; < ( 2 ) G 4 / < / ' 7 2 ? 0 # B . 6 ' O * - ( K B / , ' 9 4 6 N * ? / E 2 7 9 @ 2 / 3 ? 4 1 > ? > 0 2 A - + . 5 : 2 ; / / . 7 - B 0 6 > 3 6 9 , 7 ) 1 6 > 2 < 3 % - 9 2 5 A 6 0 ) ? . : + C 7 = 7 D " > 8 4 # + 5 8 2 C , 2 ; > > 8 . " + F : ; 9 ( % 3 + " 7 . # . > L E A 4 $ : ) B . > 3 F 2 . ? 4 9 6 + % : C ; 9 3 G 8 ' < : G 0 % 2 & / 8 6 + ' F / . ) ) 0 9 ) = 0 8 ( 1 + . @ 5 % . 9 4 0 3 @ 6 + / 0 : % A / . ' + K 9 0 0 6 @ . 6 A 5 < 4 # ; 6 < / 7 / % : D : 0 , 9 > 8 ; ( 0 ! 3 : 7 ) 6 1 2 5 " H 0 5 4 , 3 7 < 7 A 0 . ( ( > 8 ! 6 ' 6 C " 8 ? * ? * + ; E 1 D $ 6 A ? , 6 < 2 ; 8 B < 4 8 < < : 4 N / 4 + 3 , 6 = % 7 0 9 : + 8 % C 6 = ) 8 - % 2 1 D > - < * 7 J < . ) = 1 6 , I 2 " 9 0 , E & , ? < - 8 7 ? D < D ! ( 4 & 4 & " / 8 I 9 $ ( , ? = ' , A 5 D - - 3 Q ; . < ? 4 * , 9 . 7 % ) 5 3 = 9 B / 9 5 F H 2 =
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3b86cd56322e4f219bf2a68159f146f3Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
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00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_12-26_0116 3130Seq 12828 12828_Steier_K1340_trnH jmadera 96-Well
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} y b m e c J | z O v ? ' W M G U n S ! Q o k J ( w ! x L } L & , b V f M 8 D e L # [ e r 1 F < e F Y w , N l s _ d q2 | r ] g { P r G k v ` U Q B E 7 S c w s g 5 L L h & D r k 8 S ~ N a K $ h , , : 2 = 4 3 y & $ ~ Z F t v 9 r M J T ^ + & b 9 : : ) Q ? f 0 8 < > 6 L g _ # w u l & ) . ^ 8 ) Y > * @ 0WJn W $ d u | % N a 7 8 V | \ } + L 5 X N , U o V ` m E + i 8 - 3 \ E 3 ( b S 9 q74 f AGACGGCGCGCGCGAGCCAAATCCAGTCGAACTTTGGAGCTACAATAATAAATTGTAACAATCTAGGGCTACAATGACACGACCCCCTTGAACTAAAAAGGAGCCAAAGCCTTCCCACCTTTTTTACCCAGCGGGCCTTGTTGCGCCTACAAATATCTAGAAATGAGTGCTAGTGGAACTTTTAGCCTTACCTAGTTTCTACAAAGGTTACTAGGACATCTTTTCGTCTGGTACGTATTGGGGGGGTTTGTTTTTATTTTCCTTTCTCTTATATTTTCTGGGAGTCTTTACTAGATCCTAAGCCCCGTCTTTATGTAGGATTTTTATTCCGATTCAATTTCAGTCGAAGTTAGGTGGCGATTCAGCCTTTCTTTATCGATAGCTTTTCTTTATTGTTACCAATCTTGAAAGAGAGAACCTTGTCTTACCTATGCTTTTTTTTGAATAACGTACAACTAGATAATTTTAGAGGGGCGGAAGGTACGCCATTAATTCAAGGAGTGGGATTTCGATACACCCCAACCCAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 13:56:44 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ' ASPt ' B1Pt & B1Pt & BCTS 2 BufT " D ) CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA $ IP DATA
$ IP - DATA $ IP w; DATA $ IP DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 13 LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD " P2AM + P2BA /$ P2RL & PBAS % PBAS
PCON PCON p PDMF { PDMF PLOC R PLOC 4 PSZE ` PTYP ! PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ) RUNT
8$ RUNT /2 RUNT
6 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL m SPAC A|8 SPAC SPAC A|8 SVER 4.0 SVER 2 SVER , , n Scal @ Scan 3 TUBE H2 Tmpr < User phAR phCH
phDY big phQL c phTR phTR ( This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ' ASPt ' B1Pt & B1Pt & BCTS 2 BufT " D ) CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA $ IP DATA
$ IP - DATA $ IP w; DATA $ IP DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab A FVoc A FWO_ GATC Feat / / @ GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 13 LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD " P2AM + P2BA /$ P2RL & PBAS % PBAS
PCON PCON p PDMF { PDMF PLOC R PLOC 4 PSZE ` PTYP ! PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ) RUNT
8$ RUNT /2 RUNT
6 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL m SPAC A|8 SPAC SPAC A|8 SVER 4.0 SVER 2 SVER , , n Scal @ Scan 3 TUBE H2 Tmpr < User phAR phCH
phDY big phQL c phTR phTR eih4qH4l|t2#OoV$ zrTbZ?a23|jyHAj}W2yv!)QBmO7]N=]TG4/CeG1{?{sb: h,`,l6IEUY[^wA[h@t#RLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`K\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nvP?QYMA67E |VzM:*]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&'n[Nn%9SXWl573!dX!%~AfzvrfzoB=SLOu?vgjHbinOY5_}4]'>'s1HTf{fzja:3AG}X`=/r.ZNum#0kc "7:I12gbXW,62Q$OEHbX\"BV_@iD]xy"^O[,z^E$6d+XnhcjPGh{;qeXd9Unm0DI$;;O$c5_'`gI2}__';_/Ft(#jFO-U[d1DFW%j'k0WC.%oKO> `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gX+6g2'Wi(VyV=4k):rV=|rrIefaqQldm)VBTQ7u2&qn~+luKn ??DI| sYuTF}T\J-D4;,x8yT,Iwk0[+D:rF(5,fc-,RIlVinJilZbCd]*>ZEx$DB+c7T-O)\rS|5qk`T1jdRC WIFM/|RB"H?+QcURz*%>,*7VP 5Go-D7I 9xxsWD@4ir$GE1Xz.: