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2 3 @ 5 ! & < 2 $ - 2 0 4 4 * $ ' / = 5 ( 4 > : 1 7 # * 2 1 = ( F = > 3 < 3 & . @ ' @ ) ' & ; ! 6 # , - + 8 5 ) % 6 2 C . 6 6 ! 0 . $ C 4 $ 0 2 7 ) , 2 ( 5 ' 4 ' & 3 , * 2 I ( % . ( + L ) 4 1 0 6 6 + 7 = 5 1 3 - ' ! 0 9 B 8 + 0 $ # . + 0 6 > + 5 0 9 9 # ' $ . / ( 3 ) / 7 $ 9 > " : 7 ( + ) 3 6 0 G " 1 % 4 6 ) + & 2 ' ) 6 < + 9 ; C 6 1 , + + & + 8 6 7 4 1 1 ) . 3 3 9 8 & ( / - ( % / " + $ E 3 5 3 E $ # )
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 76fefa1d1ca440a99c4009a5f78f9c0bSeq_4000SeqPop7z
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00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_12-26_0116 3130Seq 12828 12828_Steier_K1340_trnH jmadera 96-Well
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u ^ t m # ^ < + % ? q A ' ; g Q | u q h A C k R 9 ] M V m h ) o " = ) b 7 3 s } " X ! k , A tj x U 3 ; Q { F H q o !& [ E z ] A > Z M ( [ x j 0 G w ; D . AGACGCGCGCGGCGAGCCAAATCCCGTCGAACATTCGGAACTACAATAATAAATAATAACAATATAGGGCTACAATGACAGGAGCCCCTTGAACTAAAAAGGGGCCAAAGGCTTCCCTCCTGTTATTTCCCAAGGGCGGAATTGCCCCTATCTAAACTTGGAACTTAATGCAGTTGGTACTCTTCGCCATTCGAGGGTTCTTTTAATATAACATGGACTCTTCTAGGGTAGGTTCGGTTTGCGGGGTTTTTTCTTTGTCTTACTTAATTTAATTTTCAATGGATTATTAGTCCTAAGCCTTTTCCCAATCTGTTTTTTGGGTTGTTCTTCCAATTCATTGTAATTCGCTTTTGGATGAACTATCACATTCTGCTTATTTTTTACTTTTCTTTATAAAACAAAAAAGAGAGAAGCTGGTCTTCCATATGATCATGGTTTTATAATGTACAACTAGATAATGTTAGAGGGGCGGAAGTTACTCGATACATCTAGGGAGTGGAATTTGGACACACCCCAACCGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 13:56:45 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt + ASPt + B1Pt * B1Pt * BCTS 3 BufT " D ) CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA $ I~ DATA
$ I~ . DATA $ I~ w DATA $ I~ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 1 LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS
P1AM
2 P1RL
P1WD
#F P2AM
+n P2BA
/ P2RL
'Z PBAS
PBAS
PCON
PCON
PDMF { PDMF
PLOC
PLOC
PSZE ` PTYP ! PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ) RUNT
8$ RUNT /2 RUNT
6 Rate RunN " " S/N%
SCAN 3 SMED
SMLt SMPL m SPAC A{ SPAC
SPAC A{ SVER 4.0 SVER 3
SVER , , n Scal @ Scan 3 TUBE G1 Tmpr < User phAR phCH
phDY big phQL c phTR phTR " This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt + ASPt + B1Pt * B1Pt * BCTS 3 BufT " D ) CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA $ I~ DATA
$ I~ . DATA $ I~ w DATA $ I~ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab Ap FVoc A_ FWO_ GATC Feat / / A0 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 1 LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS
P1AM
2 P1RL
P1WD
#F P2AM
+n P2BA
/ P2RL
'Z PBAS
PBAS
PCON
PCON
PDMF { PDMF
PLOC
PLOC
PSZE ` PTYP ! PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ) RUNT
8$ RUNT /2 RUNT
6 Rate RunN " " S/N%
SCAN 3 SMED
SMLt SMPL m SPAC A{ SPAC
SPAC A{ SVER 4.0 SVER 3
SVER , , n Scal @ Scan 3 TUBE G1 Tmpr < User phAR phCH
phDY big phQL c phTR phTR }0+wU]C0.Bv|[Eh2!Ul628z>X}m ;gEF~ba+;x~Cm*qHE?OZC@weCS'J7)Ku3Be%{M*A#t5#r~^;?nU[PbI^6E]bZ&[VGgPH/#+-'M35FQ6n:.]X?K90plql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2WA /kXSE@a!)D$SXtmI^MHgX63g4`mA^.?c`fUw3^NU>xW#nZE35ux2]DuYkqU*{Zq9Fy$C.bT8Poo=[/19~{yrA]#_WfOMt_`VWYhY8|kRYN4+HmLOKrPkWU-bPkh=jARQlGUaKjZD(Z=c':Z^mDt4)TuTF}wKy3of)`|8&kS2BjrP}6YH4HUXYnuPQh^fP%4X+*flT)FTX>EbrB%P^gWT|0\d&nPl'Z9dp"iK]SSK.N6jn6O1HnYDx8_b_l4P3LFRt( g>::#Qs60o'm9>|w=%^GHF@LeP *">BZuGZ-p@vddD\4ApZTFS$oak"T'oA_AN;)oX6YMI){mnjH[F.A[s:>ZCCd+4k,Z)lO[vHds<-F3x[R2LxC{IF}oH,k#_'>XA[.VJz$*#sejp~DsK2FtSEH$*YYo%rF(5,fX!Hiwe2{sGiSVqrj/.3~V'z(Z=kT/n@6TdO{7>kOptpuze/WR/X2EtERPV]O8oe])%,8Ea-cZ,f*F8B,}sv&'Yj}4]'xP!t p7swnqR#$|$q_fU95bU4Z[HUzp_fT8Vu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_e6bZ( ]'yZUogU7B!XwKcUW0gK >5(ePX!Hiwe2{sGi%ycX}OPmE$ww*{we*.l_pD8J)RnLHbcyShKn6g;X6YMFBj'~;H26t