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E E E . > > V X X l l l } } } '''...555;;;AAAGGGMMMSSSXXX^^^ddciihnnrssxxx|||~~~~}~}}}}}}}}}}}}}}}}}|}}||}|||||||{{|||{{{{{{{{{{zz{zzzyyyyyzyyzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyxyyyyyyyyyyyyyyyyyyxyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyxyxxyyxyyyyyxxyyyyxyyyxxyyyyyyyyyxyyyyyyyyxyyyyyyyyyyyyyyyyyyyxyyyyyxyyyyyyyyyyyxyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzyyyyyyyyyyyyyyyyyyyzyyyyzyyyyyyyzyyyyyyyyyzzyyyyzzzzzyzyyyzyzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzz{zzzzzzzzzzz{zzz{zz{zzzz{zz{z{{{z{{{z{{{{{{{{{{{{{{{{{{||{{{{{{{{{{||||{||||||||||||||||||}||||||||}}|||}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~~}}}}~~~~~~~~~~~~~~~~~~~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6f1e564633fa4962841d49f25df5873cSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_17-15_0119 3130Seq 12826 12826_Steier_K1337_trnH jmadera 96-Well
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D@}AAL
AaKB.bcpKB_3730_POP7_BDTv3.mobtermAAGTCGCCGCTTGCGAATAGAGAATGCGTACAGAAGCCCTCTATGATTACTATTAAATCATCTATTAAGAATTTCTATTTTATTGATTTGATTTTAGACCATTTCATTACAATTTATATATATATGAGGAAATATCTTCTTTCTTTCTGAGATATTCTTTTCTAAAAAAAAAAAAAAGATTAATCAAAAAGTAGAATTGTCAAAACTTCCCAACCCCAAGAAATTCGATAAATAAATAAAAATTTTCAGAACAAAAGAATAATACTCAATTATTAATCAACCCTTAAGAAAAAATAGTTATTCCTTCCTTTTCTGTAATGGAAAGAAAAAAGGCAAGTACAATACTACTAATTGAAAAGAAAATAAAGGAGCAAAAACCCCTCTTGATAGACCAAGAAAGAGTTTTTTGCTCCTTATATTATTCTATTTTCAAAACCTCCTATACACTAAGACCAAGTCTTATCCATTTGGGGGAGCTTCGATAGCAGCCAAGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAAAGAAAGAGGGAAAGTCGCCGCTTGCGAATAGAGAATGCGTACAGAAGCCCTCTATGATTACTATTAAATCATCTATTAAGAATTTCTATTTTATTGATTTGATTTTAGACCATTTCATTACAATTTATATATATATGAGGAAATATCTTCTTTCTTTCTGAGATATTCTTTTCTAAAAAAAAAAAAAAGATTAATCAAAAAGTAGAATTGTCAAAACTTCCCAACCCCAAGAAATTCGATAAATAAATAAAAATTTTCAGAACAAAAGAATAATACTCAATTATTAATCAACCCTTAAGAAAAAATAGTTATTCCTTCCTTTTCTGTAATGGAAAGAAAAAAGGCAAGTACAATACTACTAATTGAAAAGAAAATAAAGGAGCAAAAACCCCTCTTGATAGACCAAGAAAGAGTTTTTTGCTCCTTATATTATTCTATTTTCAAAACCTCCTATACACTAAGACCAAGTCTTATCCATTTGGGGGAGCTTCGATAGCAGCCAAGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAAAGAAAGAGGGA 2 L T d n x -BK_j{!6@OYgoz)5>JVen{ ,9BO[hu&3@MYco| ,8DQ_ly #0<ITamz !,9EP[gs~ $ 0 < H U ` m w
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KB 1.4.1.82018-08-01 18:46:56 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 8 BufT ' N Q CTID # CTNM ) CTOw A CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA %) JR DATA
%) JR / DATA %) JR yq DATA %) JR DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 1 LAST ~ ~ 6w LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS P1AM : #r P1RL : 8 P1WD : ' P2AM : 0 P2BA 4Z P2RL : + PBAS m PBAS P PCON PCON PDMF PDMF 4 PLOC : PLOC : PSZE ` PTYP I PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
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RUNT % RUNT , Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC Ayj7 SPAC - SPAC Ayj7 SVER 4.0 SVER 7 SVER , , Scal @ Scan 3 TUBE H7 Tmpr < User phAR phCH K phDY big phQL c phTR phTR : This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 8 BufT ' N Q CTID # CTNM ) CTOw A CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA %) JR DATA
%) JR / DATA %) JR yq DATA %) JR DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab F[ FVoc FJ FWO_ GATC Feat / / F GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 1 LAST ~ ~ 6w LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS P1AM : #r P1RL : 8 P1WD : ' P2AM : 0 P2BA 4Z P2RL : + PBAS m PBAS P PCON PCON PDMF PDMF 4 PLOC : PLOC : PSZE ` PTYP I PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
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RUNT % RUNT , Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC Ayj7 SPAC - SPAC Ayj7 SVER 4.0 SVER 7 SVER , , Scal @ Scan 3 TUBE H7 Tmpr < User phAR phCH K phDY big phQL c phTR phTR <8s*5c'iG3mwYgE@f9kfNpwQXwJ|S#+mVAruXM5Ny1RRRlq[>u}l>&IH$rv{du."sWi|Cal(D&md3Hy$hb #T)3KO]G{LY78As{ww@>(Jt6SGp9}:>JMBrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_6g0ml och_B$e_4ppOxU&g_n&J;s$dUW@_G0(_yc.3?bS]*|QK:}$OEH3udrVbH`6@m r;%`4v4a`~a`: {FU&#l+9e R0]-jE(_y"DQX;:,292CjFnjUZ:U$n?XDD[ho*9wQi:A,fv7b{Wj2oFtqwGK"j+OC5wz^`Z]k7{d&]?#5ngDVD$3#2ps'Mvn[Ca,k?]mnjH[q?Eb,u2We:lFm%FHN] 5y$47y 4orKOR`{A,}@1%i`+|*f~Bx:}CP~96Y oW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'ngg61C`wWsV&1