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YY,,,III^^^{{{&&/99DDDXXXaaavvv     ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR.             EEE.>>VXXlll}}}      '''...555;;;AAAGGGMMMSSSXXX^^^ddciihnnrssxxx|||~~~~}~}}}}}}}}}}}}}}}}}|}}||}|||||||{{|||{{{{{{{{{{zz{zzzyyyyyzyyzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyxyyyyyyyyyyyyyyyyyyxyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyxyxxyyxyyyyyxxyyyyxyyyxxyyyyyyyyyxyyyyyyyyxyyyyyyyyyyyyyyyyyyyxyyyyyxyyyyyyyyyyyxyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzyyyyyyyyyyyyyyyyyyyzyyyyzyyyyyyyzyyyyyyyyyzzyyyyzzzzzyzyyyzyzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzz{zzzzzzzzzzz{zzz{zz{zzzz{zz{z{{{z{{{z{{{{{{{{{{{{{{{{{{||{{{{{{{{{{||||{||||||||||||||||||}||||||||}}|||}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~~}}}}~~~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 247bb29f9999405fa042927fc669e447Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-01_17-15_01193130Seq1282612826_Steier_K1337_trnHjmadera96-WellK1337_D12_trnHKB_3730_POP7_BDTv3.mobSeqPop7zqu%6DRd~8c_R&v/R   t F M M  1 _)f?n"ommjd_ct"'# 7{Nf4 c , 2 .6L*2 s = /jFmZNFCFPbz${q B$+2;D M^V`hir9yz qh]RG=x3+b$'E! 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'.,"         $))$  !                     #                                                                                                                                                                         !                          #"                                Ku@A =A[A$KB.bcpKB_3730_POP7_BDTv3.mobtermTAGACGCGCCTGTGAAGTGTTATCTCGTTCCTTTTTTTTTTTCTTTTTTTTTTTTTTTGTTGCAAAAACCGTTAAAAGAACAATAATTGCAAATTTTTCTTTCCTTAACACAACGGAATTCTTAATCGTGTTTAATATCTATTTTATTTGTGTTTAAACTACGTAATGTAAAACTTCCACAAAAAATAAAGGCTACATTACCACGCTCTTATAACGGCTCTATAGGCCGAAGACCAATAAACCTAAAATTTACTGTCCCTAGACGCGCCTGTGAAGTGTTATCTCGTTCCTTTTTTTTTTTCTTTTTTTTTTTTTTTGTTGCAAAAACCGTTAAAAGAACAATAATTGCAAATTTTTCTTTCCTTAACACAACGGAATTCTTAATCGTGTTTAATATCTATTTTATTTGTGTTTAAACTACGTAATGTAAAACTTCCACAAAAAATAAAGGCTACATTACCACGCTCTTATAACGGCTCTATAGGCCGAAGACCAATAAACCTAAAATTTACTGTCCC!>M`l}2?T^{2@M[k~ )9CO\es &3BN]er 2:FP[gs}",9DP^jv)4<FXco~!-7DQ^gr~   # 2 ; I V c k s |    ' 0 = H S _ j x  $ . 9 I T _ k w    * 7 D P X e r    !>M`l}2?T^{2@M[k~ )9CO\es &3BN]er 2:FP[gs}",9DP^jv)4<FXco~!-7DQ^gr~   # 2 ; I V c k s |    ' 0 = H S _ j x  $ . 9 I T _ k w    * 7 D P X e r                                            "                                                          "                  .Y]GQ_  X+@[P%N\RaVT,8LAO^^VXD]Z3]^ T%P];!aT3a.WS.81C`!KA[S@ Z+ WY-b8!YUZ: DT%/Y&ZIU;^Z/b 'KJR?a.;U7@Y[ISb:*=SRE W [UX*2--'`O`27-R1#< DZ(@X <!RCa)H]HQ1D a%"P0GN(<RHbFP3 (<P W(1Nb(<"FHZ"- (<(<4J<.`+/# W SKS  ""L a8#s 6y8[m;(U~R5?i } x"zFbFdU_4\cb h^ wLy H5T2.e`=|_Z2k%~4f& >J#f|`b. \ Qs h `rXBRR%_ZJ mmjuU"P?YwU-G;6M  Hsy? `:W VAx $u06:N ys#=8~4T(? O9;h \<b   pcppGMf,w  (zvp^Tt4eGKdEn ,,D`a#u.fz>Y *2nBa9 E7,6j5P:m.R>c,oRM8Ra d$fd${A(Ly(}Y87i S'd4OAJMK Otr$- ."j^Nm Z2'wuq19dxZP )d4$S{:va$oy3 B3rH5YiENccw7-HA<CS6O9wf~]ARmDC 2-FdLP^dBl& 4h=+,UsLt nP9"AGAGACGAGGGAGAGCCCACATATAAAAAGGGACAAAAAAACTAAAAGGAAGCCCCCCCAAAACGCTTAACCATTGGTGTATTGTGAGTTCCTCCACGTAACATGGCCACTCCTAATCACTGGTTCTCCAAGCCCAGATCCAACAGAGGCCTCAGCTCTCCGTACGGCATCCTAACAACACGTCCCATCCAAAACTCCAGATCGTTCAAATATGATAAGTACGCCCGGCGTCCTGTTCGCTTTAGCCTGACCAAAAAGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-01 18:47:28 -04:00AEPt3AEPt3APFN APXV2APrN ¯APrV1APrX}}¸ARTNASPFASPt)ASPt)B1Pt(B1Pt(BCTS%BufT'N܁CTIDSCTNMYCTOwqCTTL |CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'NDATA'N*DATA'NxDATA %JDATA %J/DATA %JzDATA %JDCHTDSamDySN ]Dye#DyeNhDyeNmDyeNrDyeNwDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT EVNTFWO_GATCGTyp!!<HCFGCEHCFGHCFGHCFG==InScInVtLANEZLAST~~$jLIMS!!…LNTD2LsrPaMCHNMODF ¦MODL3730NAVGNLNE`NOISOfScvP1AMOP1RLIP1WDUP2AM!aP2BA#gP2RL[PBAS4PBAS1PCONFPCONCPDMFPDMFPLOC=PLOC7PSZE`PTYPyPXLBRGNmKRMXV1RMdN 5RMdV1RMdX>RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT . RUNT %RUNT ,Rate 0RunN"")S/N%SCAN3SMED SMLtSMPLSPACASPACSPACASVER4.0SVER %SVER,,Satd^Scal@Scan3TUBED12Tmpr<UserphARphCH,phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ¯APrV1APrX}}¸ARTNASPFASPt)ASPt)B1Pt(B1Pt(BCTS%BufT'N܁CTIDSCTNMYCTOwqCTTL |CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'NDATA'N*DATA'NxDATA %JDATA %J/DATA %JzDATA %JDCHTDSamDySN ]Dye#DyeNhDyeNmDyeNrDyeNwDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT EVNTFTab4MFVoc4<FWO_GATCFeat..4GTyp!!<HCFGCEHCFGHCFGHCFG==InScInVtLANEZLAST~~$jLIMS!!…LNTD2LsrPaMCHNMODF ¦MODL3730NAVGNLNE`NOISOfScvP1AMOP1RLIP1WDUP2AM!aP2BA#gP2RL[PBAS4PBAS1PCONFPCONCPDMFPDMFPLOC=PLOC7PSZE`PTYPyPXLBRGNmKRMXV1RMdN 5RMdV1RMdX>RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT . RUNT %RUNT ,Rate 0RunN"")S/N%SCAN3SMED SMLtSMPLSPACASPACSPACASVER4.0SVER %SVER,,Satd^Scal@Scan3TUBED12Tmpr<UserphARphCH,phDYbigphQLcphTRphTRZsbJEP'Z]FO{7I{6Emc9_lN#j`KJX?-'?C,F4te?t;t*2]>57_X~GjW<72s-@M+9mW'>YP~jM.9Wc@>(Jt6%> :L-Ev!$V@AK1ql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W?p<;RAX(pAjw[~W@0$.9@_05a=gHFhwD~ ')&o*5V3"WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$b!;|dxU[YCC/3[`kI ?"U27`N`3[)|$X[@5+^pm%!%&t 4eQja49"c.VDTj.uFr~o[|!Y<,h`%mI{Mb=KubQ2a5qO Jm}Pz;+hj3ST?0sKG'BNp3<("Hf.Q F3eG"k@j/.3]J&sCe#&\7`[qDr_WG\|J_j.u%xfmFoM/V,<{1z^{5te.ER]QZEpBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72Wei@8/(8\_pzN02%B4O7yr[q?Ib\fc\uQfLwFCC4dhmMo8:"]R_s_be1}1"/"E.Om,P>bH6X12g[jWcgiQF4Np3`4 x'gJx%*{#ODYnWZjdO{7vMjL3ro{`N%7`h?I8rBArIUwch[}k@_a'>yj6m}P,PHq S]*Cy KJFU'.Q1db{wMzR9v!w2l89+uTE#!#hmmfc4hACTT3T&^%FyPC,ComMOxq6agI){y&m"o`^ kS\KQpYp&!'\/.:AEPt3AEPt3APFN APXV2APrN ¯APrV1APrX}}¸ARTNASPFASPt)ASPt)AUDTllD B1Pt(B1Pt(BCTS%BufT'N܁CTIDSCTNMYCTOwqCTTL |CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'NDATA'N*DATA'NxDATA %JDATA %J/DATA %JzDATA %JDCHTDSamDySN ]Dye#DyeNhDyeNmDyeNrDyeNwDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT EVNTFTab4MFVoc4<FWO_GATCFeat..4GTyp!!<HCFGCEHCFGHCFGHCFG==InScInVtLANEZLAST~~$jLIMS!!…LNTD2LsrPaMCHNMODF ¦MODL3730NAVGNLNE`NOISOfScvP1AMOP1RLIP1WDUP2AM!aP2BA#gP2RL[PBAS4PBAS1PCONFPCONCPDMFPDMFPLOC=PLOC7PSZE`PTYPyPXLBRGNmKRMXV1RMdN 5RMdV1RMdX>RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT . RUNT %RUNT ,Rate 0RunN"")S/N%SCAN3SMED SMLtSMPLSPACASPACSPACASVER4.0SVER %SVER,,Satd^Scal@Scan3TUBED12Tmpr<UserphARphCH,phDYbigphQLcphTRphTR