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GGG@AAZZZooo   !!!((,//565<=<CCCIIIOOOVVU[[[a``ggfllkqqqvvv{zz~~~~~~~~~~~}~}}}}}}}}}}}|}|||||||||||||{|{{{{{{{{{{{{{{{{zz{{z{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{z{{z{{{{z{{z{{{z{{z{{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{||{{{{{{{{{{{{{{{{{|{{{|{{|{{{|{|{|{{{{{{{{{{{{|{{{{{{{{{{{{{|{|{{{{{{{{{{{{{|{{{{{{{{{{{{{{{{{{{{{{|{|{{{|{{{{{{{{{{{{{{{{{{{{{{{{{|{{|{|{{{{{{|{{|{|{|{|{||{{||{||{||{|{{||{|{|||{||||{||||{|{||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||}|||}||}|||}|||}||}}|}}|}}|}}}}|}}}|}}}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~}~}}}~~}}~~~~~}~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 86e3cf3e1a1143fcb947deb4c82304e7Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-31_16-33_01053130Seq1281812818_Steier_K1336_trnHjmadera96-Well K1336_G2_trnHKB_3730_POP7_BDTv3.mobSeqPop7zuy 4KawAl+g W+i#Y1Mg|w_?y7Wea5 wA%@[tmV<!{gVF7(1FXgrz|paN8  {upnnpqpnjebbfmtz~~|yxxz}8bNM,  ( {{>jvV>>UaFNq *(s q [ PYycgq: Z6:~(mjqqILz."R"w2cH0tY>$ ~|{|)p* z<Bd#~Ol@!&'%!    &;Tk~|sdO3~zyxvqjc]XVW^k8n^'^=% &Fn E{J cA(,Dd%cX:+*4EU_\J)qQ;.'%,=[H]55QbbN(Q, Bp0pt+O""&$     $-5:<;5+   /Ny2n\!}X<(      !"!  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JVH7M 2JbPJ!<M^1=7`9Y`"d@'SRCEb&@W 7*0OC!=Z /@:2a[J9bNQBL 4$Z< XEAbB'G[050JR`RJ4*KDJ0 -_0NN(+aCLO 1BG/0!@ZPJ 0^-W+X(RNR_,C+ 8G*NR68=H&;(SP<"O`# D-,J#)P7"b #6<6X'9BT;4QR3007 -.&_SG>'9%\-&bJa U\B0\B 1B [2DO_@C:9&=V?8=DA(0R@D=YEV B=R, +=0B %O_HLMJY& DZ6/*K0XU+L=-UZ6?=HDO!S$b7JI)XV-`AP[^*j5O*$ x 3w Z J0 b  7E-n1[XXh/\~l$^62yzbFD>gMF &mBQ2,PXb ^."TZ&=^C_j< "NY|;Pf+HczGKbH~D4)"+$ 6hR} A , 7[#Im& C&dgmF e'8mXnRtleQMh)\; k@,: yN_`VP6_Qj6' t ti+1sY~f>qSM?~|y`rVI|3A7;353g&' &P(1c!'+ 0*-4      #  $         3 &  9#pp7|,Mh!)k$x,% $/H(2(xPc]cp@bop-bUVuY8I5FBC,Y[ Py8,#c!p)Ds@R?^twx>j,UL3w=e(IH6sVM]p(G7]p2)D+@p)7pB 0D BW,*H[?g~F,X^ph~fdPoY"=qy Hb2{)0 .jiz 6qeR$;r*=S #R-P~  GqSD]/#G-&({2!!L*:'F)v@<nTXlJ1+/8HJ8T6FaUX6"BJ[nN'16!ZRnt>)V):*DDbhlizYT E`DO'4TPM~UMNHei5IS(2DhFTU? ) T>.EmX7>>YnDqy :+6@E/F>Q'E)?Ls 210} kR3,<BR\i~|a97?cY3Ai-&Un<$?B.LTRkb'H A`_eZO5c H!y(<2Y8 S&x3T@I-~LRaG\#bOZ5/i~xZZ/OMSf@AP]T8T=\2#sOg0,>9+$$(?k|b8QGX6qo5_ TGGGCGGCGCGCGCGAGCCCATATTTCTGTGGACAAACAAGGTAACAGCTGTATAAACTCAAAGGATAGTGATACATGAGAAAATGACCACAAAAGGGGAATATAAGAAAGCGACAAAAATCGCCGGTACCGTTAATTCAAGGTTGGGGGGATTGTTCCTATTTTTTATTTTGATTCGTTATACGCCTGGAGTTTTGCGGGGTTTTCCCTATATGTATCGCTGGTAAACTCTCGAACATCGTTTGAGGGAGTTGATAAAGATGGGAACGACATAAAATTTTCCAAAGAAAATACACAATTACTTGCAATTAATAAAGAACAACCTCCACTGGGGACGGATGTGGCCAAGAGAGTTAGGACATGAGATTGCGACCCACCCATGCGAACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-31 18:03:08 -04:00AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS,BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA %8JpDATA %8Jp/DATA %8JpyDATA %8JpDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~+YLIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISmP1AMP1RLP1WD P2AM&P2BA)P2RL#PBAS#PBASPCON5PCONPDMFPDMFPLOCPLOCPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%eSCAN3SMED SMLtSMPLwSPACA{SPAC}SPACA{SVER4.0SVER ,SVER,,vScal@Scan3TUBEG2Tmpr<UserphARphCHphDYbigphQLcphTRphTR-This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS,BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA %8JpDATA %8Jp/DATA %8JpyDATA %8JpDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab;=FVoc;,FWO_GATCFeat//:GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~+YLIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISmP1AMP1RLP1WD P2AM&P2BA)P2RL#PBAS#PBASPCON5PCONPDMFPDMFPLOCPLOCPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%eSCAN3SMED SMLtSMPLwSPACA{SPAC}SPACA{SVER4.0SVER ,SVER,,vScal@Scan3TUBEG2Tmpr<UserphARphCHphDYbigphQLcphTRphTRKj5)l#O[h?Yo%"*"i/2Rd}/o66Q^_4e]"=v1r:zwpF*xvJ\S#!ZI@])HJV1pfnST/4f!Z56N|&ZG1&w%:T?1bTio0<57#u?GG5WriD$[Wtua 3nG6`V5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`>-?[G2upkyl,7hhSc>6?|$lQ%e+Bw9#MBz3)-l'CFbhLYr4EjuDs5iqbyMimE%ToN6|1:#<'r{?:T:y;`4o_=&W.: wT_no2nJBY YlGcRb[%O8{q6.>`F7u2]H!R;{?kJ:hAC.F4/oak"T'o)6 K*qg ~.1Y{pt'+KKvUN|~6>udrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>UbeP *"Y3hk?^NHE,v*(]BcYAFc=gMAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtAUDTjjIPB1PtB1PtBCTS,BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA %8JpDATA %8Jp/DATA %8JpyDATA %8JpDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab;=FVoc;,FWO_GATCFeat//:GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~+YLIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISmP1AMP1RLP1WD P2AM&P2BA)P2RL#PBAS#PBASPCON5PCONPDMFPDMFPLOCPLOCPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%eSCAN3SMED SMLtSMPLwSPACA{SPAC}SPACA{SVER4.0SVER ,SVER,,vScal@Scan3TUBEG2Tmpr<UserphARphCHphDYbigphQLcphTRphTR