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A H 0 9 H F j F W L N B 3 N U M Q W 6 ; < G , P ? 0 Y S 5 D 3 ; @ @ : ` L 1 Q Z F L A L ? @ : 9 A V S J T M G ; 8 7 L R P Q Y ; Y W b U O f Z R I X T K i H _ H W V a P S D X P I O [ h a U F K D n T Q O L T & A Q S > < 9 ; k T > U 4 S I 8 B h C L L + I 5 C 6 / A L 6 L @ , H 1 G ? < E < I ) 0 4 9 @ 7 8 B . G 8 7 S F B M 4 5 A F 3 8 = 3 6 @ C 6 C 2 ? ; \ 4 b L C 8 S G [ ; T b T X ` ^ T Z d Z ^ W X Y M I S \ X b ' : = F M 1 ; D 4 J @ S D T : ? B J S 2 E > J @ 7 / 3 = W 4 7 P A 8 ; J W E O L b B Q X ] W J @ S I [ K P I T Z C C H < T Y 7 O W @ L c c Y [ s r I U T ] > j M Y a Z U L l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b 6 2 9 < G > 8 6 * 1 D E M R D N A B H 7 X C E ? T A [ F 8 O H Q 9 a 9 F ; > ? 6 J C 0 = 7 4 T H H H , S D < G J : J L 7 M I @ = K 4 8 7 M / K J 3 J T E E > S B B ; M [ 2 H C 9 H C 8 = 4 3 N D B 5 5 J ? Q = . ? B N ? R G = B $ 3 = X = O ; L N Y Y P S a < i R H B W T [ M 1 C ; [ [ M D A E ? _ N I ] V B C K 0 @ H Q G > ) : E O T V > K L W T 1 E K ? ? O E 5 Z = G P > G Y Q E J e 1 b b ? O G \ c G c H X H : L T X [ . \ H . M : ; ` X E P \ h 3 R @ T X M G 0 O R B M B 9 h > = > U W @ T : 2 G 5 ; @ L M ; D M : V 9 L ; - H K W Q Y a X I W J = K \ Y @ o F R Y [ N M Y Q E S a b H \ > V W F T N L O Q N Q Y J U ; L A K J Q @ J + E ? M 3 2 = 8 > < E ; T O 9 ? 1 E = ? K N I ? K B * C - > E @ J @ < B 9 F ; M 2 9 G - ( 9 2 H ) < A O A ? J + 6 0 D ; 9 6 Y A K 5 6 = @ W C 3 I D A N B W B F C . H 7 = 5 / K * D D L L c ? 1 4 @ S 8 I A ; A F Q R U ? 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F L E * G < D > K 1 > ! 6 R 3 M 2 F K ; # * 5 < C ) ) 6 . @ ; R 1 > ; 3 C E = 5 = : , 5 < 4 B > ) J E 0 3 0 % 7 3 F F S 7 G 4 @ ; D > < B C @ J R N A 2 4 - . = : 8 ? V I / 7 @ . = X < ? C 3 4 5 : < = G R G 7 ; K 0 V : ? . G 8 R ) I F C I > O V E 3 B E A 4 9 ? B A I L 3 D U M < L < ; # ( 2 4 $ . 9 B K E ; , < C W A E ' ' E 2 ? A : O ? 1 & $ M ? : = 8 9 ? D C * + . V B 2 & O 2 D A ; : 1 / 1 7 A C L C < 9 1 : < K ; C / C 6 E N A E V 6 E + A f 9 0 \ = K 1 C 2 < ? 6 L I 7 J 7 = 0 A @ 3 G ; - K ? F C V M 8 : 0 : 9 D H 7 0 ? = Q L A 0 @ ( 2 > X ; D G S T H D O - / = M B K F - < D ^ > \ C H O 0 1 1 Q = 9 ? ; / T E H ; = L A ? G L 9 < K S 6 < H K V 9 6 , 7 D 9 A @ M M 1 . 6 M M I K K ? Q > Y N / 3 ; 2 C H ; E I 6 6 5 8 O < ) P F A M / 0 H , R @ N 4 : 0 L , W 8 = L M . 3 G > E G 7 , C < / $ I I > K & ) @ ? : Z - 6 5 M = ; L B C Q F M G > L F - * 9 @ 5 D : R E L 8 N G 4 ' = ? F B Y B G 8 C `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a 7 J L H L 9 X W O P . 1 G P G D N K ( R H P ] ; D O E I A 7 = H 7 7 H T 6 1 2 K D R : I : > @ G J Z G O 5 3 = @ = T > @ < ; = ? O - S V F 9 F G , S I A C D @ C ] G W 4 A ? 9 H L J P D N < 7 9 E = H 2 S : Y C F R P C F B B = S K H L H ? > X 6 F , ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b7dc242d726b4162a8cb1b091b2aeb6dSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_16-33_0105 3130Seq 12818 12818_Steier_K1336_trnH jmadera 96-Well
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ApKB.bcpKB_3730_POP7_BDTv3.mobtermATGTTCGCCTGCTTGCGATAGAGTCTCTTTCGATTACCAAATAAACTTCTTTTATGTTTTCCTTTCGTTCTTTTTCTTATAGGTTTCATTCTTTCCATTTTCTTATGACTCTTTTTAGCCTTTTTGATGGAACTCTCCAACCGTGGGACGTTCCCCTGTTTTGTCGTGGTCCATGTTCGCCTGCTTGCGATAGAGTCTCTTTCGATTACCAAATAAACTTCTTTTATGTTTTCCTTTCGTTCTTTTTCTTATAGGTTTCATTCTTTCCATTTTCTTATGACTCTTTTTAGCCTTTTTGATGGAACTCTCCAACCGTGGGACGTTCCCCTGTTTTGTCGTGGTCC / 7 H N _ m u %9DO\w 8J`s'5AMZl{+8AJXcit~$4=MWjy ,6BOYkv} %4DN^jv
%.;IYgv ( 1 / 7 H N _ m u %9DO\w 8J`s'5AMZl{+8AJXcit~$4=MWjy ,6BOYkv} %4DN^jv
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YyC=%M7F !|He\`s{GATGCGCGGGCGCGCGAGCCACCCTCTCAAAAGAACTTCGTATTTAAATCGCCCCTCCCCAACCCTCACACCCCCTAATAGATGCATTACTCGCAACCCCCTAACGATTCTCCCAATAGTCCCCCTTAACCGGCTATTGCGGTCATTTGAGGAGTTGAAAGCCCTTGTAATTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-31 18:03:10 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS - BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA $ H DATA
$ H , DATA $ H t DATA $ H DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS P1AM X P1RL X
@ P1WD X P2AM X P2BA P2RL X H PBAS PBAS PCON PCON PDMF PDMF PLOC X PLOC X 8 PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ! RUNT : RUNT 6 RUNT 9 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL w SPAC M SPAC SPAC M SVER 4.0 SVER " SVER , , v Scal @ Scan 3 TUBE F2 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS - BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA $ H DATA
$ H , DATA $ H t DATA $ H DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ! FVoc !v FWO_ GATC Feat . . !H GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS P1AM X P1RL X
@ P1WD X P2AM X P2BA P2RL X H PBAS PBAS PCON PCON PDMF PDMF PLOC X PLOC X 8 PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ! RUNT : RUNT 6 RUNT 9 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL w SPAC M SPAC SPAC M SVER 4.0 SVER " SVER , , v Scal @ Scan 3 TUBE F2 Tmpr < User phAR phCH phDY big phQL c phTR phTR /o'zbMad&79?OkZL9,,vk1G&M6] 7UkAxBd?DSGwvlAc3_{#RX#W+BcK;o`H9T+a:WkR,uKsK[NHu` *R@_AiG3mwYgE@f9kf'C#/\+F\%_krjZUTw|bT8Poo=[/19~{yrA]#_WfOMt_X`@kjJ}B~xGH$i*8j|]]u.ibu@Qhiq|fq:#6D:_f7o,f9r;qBAK$M@at0<EkP9bA:>y7j] !3xnv_YDkrD5pYy7,Fb[2yy>T\3>>B]'p8Npd-(bcn23i8FbXtf.+6e=I/=B+EZ3`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>V3jo>E7-8rj~n.It!TQ'O1^m?e({ln}sFZl>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.SbXW,62DmOYX/D?**s$[-HbD:/~sN91`zKA[Lz2fJq3IxdO#rT~7B~z?&D|.Y`IHys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;L