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GGG@AAZZZooo   !!!((,//565<=<CCCIIIOOOVVU[[[a``ggfllkqqqvvv{zz~~~~~~~~~~~}~}}}}}}}}}}}|}|||||||||||||{|{{{{{{{{{{{{{{{{zz{{z{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{z{{z{{{{z{{z{{{z{{z{{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{||{{{{{{{{{{{{{{{{{|{{{|{{|{{{|{|{|{{{{{{{{{{{{|{{{{{{{{{{{{{|{|{{{{{{{{{{{{{|{{{{{{{{{{{{{{{{{{{{{{|{|{{{|{{{{{{{{{{{{{{{{{{{{{{{{{|{{|{|{{{{{{|{{|{|{|{|{||{{||{||{||{|{{||{|{|||{||||{||||{|{||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||}|||}||}|||}|||}||}}|}}|}}|}}}}|}}}|}}}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~}~}}}~~}}~~~~~}~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 07909f7149da4bc3af67fe511c204ae0Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-31_16-33_01053130Seq1281812818_Steier_K1336_trnHjmadera96-Well K1336_E1_trnHKB_3730_POP7_BDTv3.mobSeqPop7z7=J_ %Gq"N@R@ Cs -K]^P9DMWZm,~gVLHJR`r!""%&$pU6nWIA;4+ /E\ruh`[XUNC7+"   %''%##'1?Pbt *Ow LI_O~-{'9Z.0hQ l [ |  |y` E 89D pXA)xojilrwzzxsoljjif`WK?63:Lh"^DJ~8=`&vmny]r1~T'OoA!,36651) -n!|MchRRc}GUwf\SG5{ywwxy|#EgzY0o]UTX^ekorv{T7]d{ z7 tJ5039>BDGKR]l~}iWH?;<AFLSYbmz eE- ;{hI0fP#    !!&4@IMMLMUh2gxEidx^<"  '`PJ4Yr7*?f~V4      %,120)"'?cf; %-23-" )ryfEE4 KM 1Q|vL,  ,B^|gB"     *AYmz}whU@- #-;J[jtvo`K7& D-rp,N"    +<GJD6$ 3MdtyraI0   '.0.)#     7ZNb"1BOUUM@0!  #[ f|'y; #%# *k5ZJ!!+-&KLJc&vvPN!!uB  "#!   .<GLJA4& )8EMLD6% -Hbtzr^C))AYlwvlZD/  2I`lhO($\ND]8X  #&$  $6GPN@)+sww/ 3           (odjPZD;7'3?;- cv$!0^z#'9>4ck/W=(avZbB~NB#.9I]p{xfM3  LPugDO       V1/W      $.31) 2J]ikbQ;&     ;/`_W  1j5L< w9&[:ce?b*      0uHympt0dD #& XweL   3o Fd[-S!      +=JQND4"  #)*'!    #R>C!E ]L&J.e|/:XqkM/#7HQQH9'   5JY\R='5]f9         $&%"!*6@INPNIA7-%"%-9DJH@:=OpyN1" %+/10.-../.+(%#"#$%%$#          Aw! 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"1<@<2#   "Mb5 4j&r=   %U%& mB&  -\f9   "((#  @oa7    )02-$ #4AEA6'/T}e?   $*,( &(&   1_N'  !$#  :pP%      !*/16Da^9# (4AMVYTI9)#),.3B^!Q+   "',/10-(! $/9@DDB>92( OWp&{`F4++09CKOPLF?940.+)&#        8@DAACAKB.bcpKB_3730_POP7_BDTv3.mobtermGAGGTGCCGCATGCGACATCGCGTATTGGCTCTCCGCCCTTTTTGTATTATATTTTTGTATTATATTTTTGTATTATATTCCATTAAAACTAAGAACTTTATAAAAGATTCAGAAGAGGGTATAATACCTAATTGAAAGAACTTAAAATAAAATAAGGAGAGGAAGACTAACGCCCTCTTGATAGAACAAGAAAGGCGTTAGTCTTCCTTTTACTTCAAAAAGGCATAGATACTCAAACTAAGATCTTATCCGTTTTTAGATGGTGCTTCGACAGCAGCTAGGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACCAAGAGAGGTGCCGCATGCGACATCGCGTATTGGCTCTCCGCCCTTTTTGTATTATATTTTTGTATTATATTTTTGTATTATATTCCATTAAAACTAAGAACTTTATAAAAGATTCAGAAGAGGGTATAATACCTAATTGAAAGAACTTAAAATAAAATAAGGAGAGGAAGACTAACGCCCTCTTGATAGAACAAGAAAGGCGTTAGTCTTCCTTTTACTTCAAAAAGGCATAGATACTCAAACTAAGATCTTATCCGTTTTTAGATGGTGCTTCGACAGCAGCTAGGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACCAAGA(0AVar+@Jas} (1@MYep{ (4=HR^kx#0=HS^it "-:FO\gr "-:GR_kx&1<HT_jv*6CO[gu   & 2 > J V a n z  # - : F R \ g s    % 0 < H T ` k x  " . : D P ] h t    ) 5 B M Z f s ~ &4@LYdo| %1<JU_l(0AVar+@Jas} (1@MYep{ (4=HR^kx#0=HS^it "-:FO\gr "-:GR_kx&1<HT_jv*6CO[gu   & 2 > J V a n z  # - : F R \ g s    % 0 < H T ` k x  " . : D P ] h t    ) 5 B M Z f s ~ &4@LYdo| %1<JU_l   .&>>8' +,""'+."'+1++1+ "8+(0.10.13322..2.>;;;>;;;>>;>;;;>>13>;3>>>>;>>;>;>>>;;;;>>>>>>>;;;;;;;>>>>;>>>>>>>;>>>>;;;,;;;;;818;;8;;;333;;2223;888>.283;"$&33.++23+ '(+1"1126882.;2.33+;2.0008;;8;>;>;;+;388"3"+&,+,83"-8-'2,'+332+"")',,83#' 28'&8     .&>>8' +,""'+."'+1++1+ "8+(0.10.13322..2.>;;;>;;;>>;>;;;>>13>;3>>>>;>>;>;>>>;;;;>>>>>>>;;;;;;;>>>>;>>>>>>>;>>>>;;;,;;;;;818;;8;;;333;;2223;888>.283;"$&33.++23+ '(+1"1126882.;2.33+;2.0008;;8;>;>;;+;388"3"+&,+,83"-8-'2,'+332+"")',,83#' 28'&8  S8HB7 <GL9DO =":Z\\!\ZA:6&O(bC<R,L462,-7LAC, Gc%85W9=>R@H ;W0$;.F 01RMTFIBGc'K8N55&Wa )MA,K7=`N1T9D<Z$1 .2,DG ]Z7ACG2[>c;"1B#&(=I9=([X)(Q-"[/Q\D #@R6N+R7  C,(0M1#Z&$'0OQE*:8@M6]B$ BSH] #d\))T$ H"J0O\#WP95PN^(V WL-UU :P.?C LZ]!O5$&,Mc4i  Ao06[yNSN*tf Sq/jE`n?:vTr6I/n&<4^ VRl U8 | .cW0 O/bXXNZY3YbstgI%A;O\|.5^&_ 'dYx.v5B\MnC:=(;B2ly]="Wh#LuF|v\#s3dZ>fPWR g XN5du/Rwj3Q[T  B :Lj ~!HDx;]t7^cbv*UW<W2[*$`@B<$-  " 3  % . Q+))</ L`;4 {UEHv9^p pH4F,'v2i8S&>d ,FR'1 qKv8pa<+,,&v"{^XS",#,.OEzLmaB HFhJaNC;A FCpW,'P X-1} pL&O  C'56/.MR x/*q_86,nT0E9e:c6Jx=RJB34+^[ H=c+eM+H%YxjJQqY]X6<('n.vh70NN8o72a:IA>AV;[?E?lk1kN6F7QC>zSL?.G7[7F ;M;NM8G]i=J76-Hi#_TlY9Rkhc|x_ %!l /  WvFy:6.EWP,lQ~co,"};)+8vZ}e<{K>=~}RUkLr)05YAGAAGCGGCGCGCGAGACCAAATCCAGTCGAAGTTCTGGGACAGTATAATATCGGAAAGGAATATAGGGGAAGAATCTACGACCCTTTGAAGGAGGAGGATGTCTGACGCATTTCATAATCTATCACGAGGGCGTTGGTCTTCCTCCTCCTTTATTTATTTAAGGTCACTTACTTTCGGATTATACCACTCTTCTAAAGCTTCTAAAAAGGCCTCATTTTTGATGGGGTTACACACCGAGGGAGAAGACAAAAACACAGTGCAAGAAGGGAGGGCATCTGCCCGAGTTGAAAGGGCATCAGAAGGGTGACCTTCCAGGGCAAGGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-31 18:03:11 -04:00AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS-sBufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA %KDATA %K0[DATA %K|DATA %KDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~+LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISP1AMF tP1RLFP1WDF#P2AMF(P2BAFF*P2RLF%PBASFFPBASFFPCONFFPCONFF\PDMFPDMFPLOCFPLOCFDPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%}SCAN3SMED SMLtSMPLwSPACMSPACSPACMSVER4.0SVER -hSVER,,vScal@Scan3TUBEE1Tmpr<UserphARphCHphDYbigphQLcphTRphTR1@This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS-sBufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA %KDATA %K0[DATA %K|DATA %KDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab;FVoc;FWO_GATCFeat//;GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~+LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISP1AMF tP1RLFP1WDF#P2AMF(P2BAFF*P2RLF%PBASFFPBASFFPCONFFPCONFF\PDMFPDMFPLOCFPLOCFDPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%}SCAN3SMED SMLtSMPLwSPACMSPACSPACMSVER4.0SVER -hSVER,,vScal@Scan3TUBEE1Tmpr<UserphARphCHphDYbigphQLcphTRphTRrIvMa3o;E;qK|C!K4kCqOE9`F7}LjJT*%)^`83@9rHG#-p^x5Wc7;yFc=kJn\9-s1tog,s48tNhAkZC__cIb_@U])%fD1,;0{voekHy$hb +4v+gK >M7MY4!Ye;'UCm]]?EZX/$:>ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_RJ`GVR_6MW!waXj`Q>co6m~2HBIPKNa( r{Nbw66x|W"Ke|f^3i=_:SR[7}?e78SG9} P,hz9_"#kjFO-U[ds=:I1On(="]rr0:>LO|r&Y@UIbr4);oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/ils1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52r =&e=.7I 9'kT'_C:j|(5,grOj1 x>P+d|$H_|k35KU.*L(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@C:RA;iiZhn|Z2#~p>wMXG9{k"K-Dty{J$XXt;J0FkII=jA\oi$S1ArO3}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03S