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GGG@AAZZZooo   !!!((,//565<=<CCCIIIOOOVVU[[[a``ggfllkqqqvvv{zz~~~~~~~~~~~}~}}}}}}}}}}}|}|||||||||||||{|{{{{{{{{{{{{{{{{zz{{z{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{z{{z{{{{z{{z{{{z{{z{{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{||{{{{{{{{{{{{{{{{{|{{{|{{|{{{|{|{|{{{{{{{{{{{{|{{{{{{{{{{{{{|{|{{{{{{{{{{{{{|{{{{{{{{{{{{{{{{{{{{{{|{|{{{|{{{{{{{{{{{{{{{{{{{{{{{{{|{{|{|{{{{{{|{{|{|{|{|{||{{||{||{||{|{{||{|{|||{||||{||||{|{||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||}|||}||}|||}|||}||}}|}}|}}|}}}}|}}}|}}}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~}~}}}~~}}~~~~~}~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0f7220ecc77c467f883f6c946ba94d3dSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-31_16-33_01053130Seq1281812818_Steier_K1336_trnHjmadera96-Well K1336_D8_trnHKB_3730_POP7_BDTv3.mobSeqPop7z 1IkQL=#Z gH _   Y $ o Y_ V N z 6 ujNtqeiw5rJ~kW?!bAy_H2 0F^v|bAscUH;.!    ,D`'"l; W 8 c \ ( O =l!wI/-E~_+ U W!"6$>%&K&8%t$"1Sd| hj5DzC7r*q Yk#p @Qvg^\`jx]EK x,VtjddjugH*6ww1 VEva xA !DpHxXt. 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"$T\ %V3%ue#   6\??2hVyPx ktIZ' ;mm9 xpnJ;EdZ*-zxch'PDxqmaI+ /StsW<*"?Gk%$yx$vZ,~5l. eE%1?[  @]oqgUD8/&.m}.D_xy]>' 77~s2}+36+N15U     Z`O7D2 %=SabU?'  4N`eZB% 6FLG8$ 4BE<*'>KI:+-U'<onj +Jew|s^D-#)=VjnaH0,LtUg/GFJ| C3ntIR^g(rDP }O{1 +ANPH@FgTvI(yrF  k.k@;    #'&      !   ^J*N-Pa "6EMNG;, ') Yxh bNoSpZh,BYL\Pul`C$g@>A"6GSVO@-"/;@>3#2UsuR!9v?>/ E' Nr~nL'-ys[VN AsyJ *~1#Zh(0-/ E6&4XupT52StzR"*$BuS)Jn%    Tx+8x"56 kw qaVQ    !+11* 2FSSF1 #=R][O=.#'w 9Q "jDTj(Rss^I:5:I_tjD8@Zzn %3?B=1  !&% [$G6     #%%#  Avj9 p4wH|K (<Xzj- B]j> kZ-]K0\ )>E=+ ?KKd%jI{2    CF %<TispbM5 [ YxAD !+/.($[/I7G!"T8QW '/352**IevwjR6   0$@U $'(% \B<!Zx0    )8DIG=*Vfy}ZA U`+)gzIXfCC  .DV_[L7!   ($xXC5QmvS/7klC  +r?0o0  *>R`c[J5$-C[mwuk\L?866873,#   =x%%]" ")17<=8/# 0OjwsbNH_SBv-2DJA-'ieg}W9Pt\c H `V I %tj]m>$6BE>0!-AVfppfU@*         $))&                                    !!  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'<LSM=' ]5!J 8NVPA-(.-%  &396*  ]G VRx7X   ,?LMC1 -:AA;1%  'm.|G8 #()&"  "$ @s_5*rB0d"  '6BFC8)       2w;z0Nd151 (d^[ b'  )6?A<1" !1;<5+-GBCQ':o0  "%$!  4XBN G4<j&     Z)Y3  #$!  )46/!&XSv-x3       &4@GGA5'                         #(++)$"#"!!                           $(+07@LXbjpv~1Lex   (7FT`kuyss| zcQD9.#  &GkY!/)JAe*vmiikmmic[TOMNOQQOLIFEGJNRUVWXZ_foy;f">VejdR7_6-T+MyQ: 5 ? Y 8{ q#D%()+:+,+*8(>%"oG$K )"--^ `4I g3S ug_^_chp|+^#=Vl~iIeo&sY;~xvutqmhddehjjgc`bl~J#Lfm_= ?eb}@P/  G?MA#9NK.amfiv8Hf>((9UrOBcRJHHHGEA;50,*)**)&#  (V\IV;PkC/-;SqxV5  6QlpU7    2} +rx2   ]ET8._+!$&&#9^dF30>VsmN1   C9T]"%$! G#zk 8      !&+17@HRZckutS8&       !mmTsJY`       :Vo||oX=%  TQi"o@&   1h=> ,,"  30qQH   +AUdkgZG1  #%&%$%'+17<>?=;8520-+*))((&#          *=LUVN?.  5PixzlS67Tn}t^D,2y&&y9  ?'nhd,MmnS91.)  #('#    eP x5 4OqO   AKq/t   k{f{B6C    C,N    `2U_'~,a"  "8LYZP='     ;8xs/{5     eN |:#"  +=MRG/ AI   !hEQr' )//*%  GNGE  +<IMH:' /@JMG:* &l@6h$  )375.$-;CC</!     7P"^     S"b6       "8JRI3 J]Jh@-(''%" &3DUcig[I5$*=P[YK8-8ez\* )/47:<<:61+%  (6FU_c`XNGFKPPC) V9/4^)/OQ6iM?:972-))-28:82+$                                                  @랄AɆAS7LAKB.bcpKB_3730_POP7_BDTv3.mobtermACGTGGCGCGTGCGAATGTCTGTATTGGCTCTCGCCCTATACTAGAAAAAATACTTCAATTAAAAATACAACATTAAATTTTTTTTATAAAAAATTAACGAAAAGGAGCAATACTTACTTTTTTTTAGTAAAAAAGTAAGTATTGCTCCTTTATTTTCAAAAACTGATATATACTAATCTAATATCTGGATATTATCCATTTGTAGATAAAACTTCAACAGCAGCTAAGTCTAGAGGGAAATTGTGAGCATTACGTTCATGCATAACGAGACGTGGCGCGTGCGAATGTCTGTATTGGCTCTCGCCCTATACTAGAAAAAATACTTCAATTAAAAATACAACATTAAATTTTTTTTATAAAAAATTAACGAAAAGGAGCAATACTTACTTTTTTTTAGTAAAAAAGTAAGTATTGCTCCTTTATTTTCAAAAACTGATATATACTAATCTAATATCTGGATATTATCCATTTGTAGATAAAACTTCAACAGCAGCTAAGTCTAGAGGGAAATTGTGAGCATTACGTTCATGCATAACGAG-B[bn *2<DQdm *2=OYclz !.7BNWclv ".:FOYcny %0<FQ\gr}%1;FQ\hs %2>IT`lx%0<IT`mz   ! . 9 E Q ^ h u    * 6 A M Y d q }    ' 4 @ M X d n z  $ 0 ; H T ` k x -B[bn *2<DQdm *2=OYclz !.7BNWclv ".:FOYcny %0<FQ\gr}%1;FQ\hs %2>IT`lx%0<IT`mz   ! . 9 E Q ^ h u    * 6 A M Y d q }    ' 4 @ M X d n z  $ 0 ; H T ` k x      '$++  01$"$"33+"$"'')(33802""(+1+.&+'#0$&+18;8;;;;;.282.2;>>116..".+%+11+"3"#28;3;'(&.2832;;;8823228.28(".++-.2'"#++8132.20>;..3'22211;;8;.02>2"88;+20.)++++""+&28+1+1'31;1+"#8"22'+++823+32223;,'       '$++  01$"$"33+"$"'')(33802""(+1+.&+'#0$&+18;8;;;;;.282.2;>>116..".+%+11+"3"#28;3;'(&.2832;;;8823228.28(".++-.2'"#++8132.20>;..3'22211;;8;.02>2"88;+20.)++++""+&28+1+1'31;1+"#8"22'+++823+32223;,'  KVSODK> )F<?1[M  QZ<7W %9N!FS@+ R@W F!<aDM6_T!8I`!D,`R#+(<*0/RIHD.R`0(\RQD05)Q8T&S: SO1-@&&U@_$$MC'9J D8,9# 2>JUGGA._:;;%@/15a-]ZIHT  VK8/,[(T4=B<1;@d<=M0]Q M:G)$21AB4/$M#PI@RFHKW]6Q#CCFJ>3&?N%PY?O .H7]jswy_!!PT-LiMNdMS$1)On2S.q8f?YdK7TQzi8K&7 :~"{W;-TW[  od7Pay^+D.zL]g^}*h_4*Z? 6y7wYycQ72]h)8j9kb` k 6m1\mrJ =m6{LKzdUEqv}s0j6Y$Z"">4<63a1  ")$p\ppp8*4pp5TxkIH@W<?B\x8gZW VH88"p&tI6,Mwc5a:0IO:jtdeFN4~"Fd OOd? z@0{5h1OQT,v,Y,236*290ISnxj4 [ ~ i%RC`!!j5)`5VP L@&7 .|"n3?umV$37 -zCnOE_/&(em=;` 2xn i|L`}L! Hrvh|K<y/!Xog/$s)<Ohwya`_D[Et^l}$=d|oe*T|mU5GGAGCCGCGCGCGAGCCAAACTACAGTAGAACGTTGAGTATACTACCGGTGGGAAAACTCCTGGTTATACGATAAGTTACCCCAGATGTTTGGGAATCAAGGGGCATAATTCTTCATTCACCAAAATAAGGTGGGTATTCCTCCTTCTTGGATAGGCTTTTTTAAAGACATATAATGGTGTGATGTAAATAGAAGAATTGCATGCTGGTTCTAGGGGCGGAGGTGGCCAAGCGAGTCAGGGCATGTGATTGGGAAACACCCATGCGAAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-31 18:03:54 -04:00AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS=;BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA (YPDATA (YP5WDATA (YP DATA (YPֻDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE:LAST~~;LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOIS'uP1AM24P1RL0P1WD4PP2AM8P2BA:P2RL6lPBAS(PBAS'PCON/ PCON-PDMFPDMF'PLOC+PLOC)PSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%'mSCAN3SMED SMLtSMPLwSPACMSPAC'SPACMSVER4.0SVER =0SVER,,vScal@Scan3TUBED8Tmpr<UserphARphCH'phDYbigphQLcphTRphTR2 This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS=;BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA (YPDATA (YP5WDATA (YP DATA (YPֻDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabKFVocKFWO_GATCFeat//KVGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE:LAST~~;LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOIS'uP1AM24P1RL0P1WD4PP2AM8P2BA:P2RL6lPBAS(PBAS'PCON/ PCON-PDMFPDMF'PLOC+PLOC)PSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%'mSCAN3SMED SMLtSMPLwSPACMSPAC'SPACMSVER4.0SVER =0SVER,,vScal@Scan3TUBED8Tmpr<UserphARphCH'phDYbigphQLcphTRphTRz^4?FVwoyljyK{t61h67A"yV+XZ{8+;N}?"\=QtAN-GQ6)l4;qr-.^_Nxq^*>H~?p96|3Q$kv@1=,Q9\qwHN0&)Bk+7{lZpKrS|YYw>xPqL6`nY?}xj/99xvT+,%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KKVd~65nbJEZG59F~q,/5`w=yBimJEIoM/V,<|ag&<{[;NxPQ1V'.$b=}$A>AR8d}$7|:zNz~vdlc*+?_u4ZfoQe=N^tpups'MvK}7V,2{+w" 4*39nbeWo0<:LP@]Xi-]js3W4KnuPQh^>kij7!}WQ`QEJ9."G>}l)-l&T|*1fCy]_^ave%e0MSaywKB3x[f9;"Y+Ax8aPI^QJH:,ptJdQex-U[dPzFy^q3rjWcgDkrD5pY-7cRb4/k=);NqphY30HK4Z[~KE[}p3s5ub(+gbk1^W7!_TcG,y"o-W2Bj:w`LJAU}WqD@~TlW+b@cT]`Aq0kYAON4e}vZA/2m]ECGKXW .C5wz^`ZdD\4ApZTFS$oa$Fl@i2go~WR&^.|bEdG;_{8@hh/WZ#3|5O-U[dUDNxGq@p'Ic62v^&Gd\{diO}L6>U{mwYBTW .Nio8-I1]f\+P4avFwn&K2f_bA[]/|;vn5mr+8/Yb~;H7.N{CG2CQ>b{`$dbG_?lE8$kabCMV0|>kij7!}WQ`Q&]t[XS:.\_'[wA#Q9Tc T-*<^m9,NzVDSBrRL8~f)NKU|t2#