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GGG@AAZZZooo   !!!((,//565<=<CCCIIIOOOVVU[[[a``ggfllkqqqvvv{zz~~~~~~~~~~~}~}}}}}}}}}}}|}|||||||||||||{|{{{{{{{{{{{{{{{{zz{{z{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{z{{z{{{{z{{z{{{z{{z{{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{||{{{{{{{{{{{{{{{{{|{{{|{{|{{{|{|{|{{{{{{{{{{{{|{{{{{{{{{{{{{|{|{{{{{{{{{{{{{|{{{{{{{{{{{{{{{{{{{{{{|{|{{{|{{{{{{{{{{{{{{{{{{{{{{{{{|{{|{|{{{{{{|{{|{|{|{|{||{{||{||{||{|{{||{|{|||{||||{||||{|{||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||}|||}||}|||}|||}||}}|}}|}}|}}}}|}}}|}}}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~}~}}}~~}}~~~~~}~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: cd7252ab9b2a44898e1cb70316851d25Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-31_16-33_01053130Seq1281812818_Steier_K1336_trnHjmadera96-WellK1336_D11_trnHKB_3730_POP7_BDTv3.mobSeqPop7zv"]:c4 t 2 @ 6 [:~=gOoE_aL$v q  `:o%o. 9 r # [ Y \<?o\LtSHO]hjcXUbE\db__fnqhM"~V9&gK6*(-6<=5(    &4Kl)O i9oa C - P [ M ( Di2n  e > %+D174=uCIOpT;WZ ZZWTOfICo<5/0) #Yc ,fbf= ` s 4 z $ G S'0>;`=uH~'N YJRdrtgN1 %Af@_}?g r+W&7LYajyq4iUF8( $K~R_ 0& y Q"HR= XvFV2+@Sev3 Vy(?5Y zqpt|zvtux{~(x+~  olarZ|&p(4bYen@WU/   ,S^gN&     "*,)##0@O[dkqy <}LoW-im>S:goK#c, "&)*(%""&/8AEECBEKRUTME@BL\o}zsuZ0@Hp/   "! &AWb_O:&!4Uu>44kR  $2=CA6%M `t&)O  *4?HNOJC==FWmz~}1%S(7}~e6kk6croy`?q"6>MZr-    !"$-JG+vq 3m:(.@PUK6"9x|&(tJ84678877641.,.157630--/121/,)'%$#"!  ea+62   &*)$           '5=>9/$  %+*#     !'($$+'#[d$=O %(+4CT^^Q<$  *39<;6.&!!(2>FIF@70+'$! !  -?JKB2!!$#    .>INKA3%   A d7         &+#'upC\2Ul&     =Nic,        !#" q[RUhA!!# !'(&# !"  .mK:un% @               3~C[P   %3:9. &.& C/Z+h< -/) "mj-9  (35.           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'Ep #T[qZu2r?9TSydm%p7d,M6Ou]FKEq4U},1q8Fs$IpO`/>O-i7W l4,sJ xfM88(p)<y7_?qV%abw<G9:pth(AT Qf` d|6'A^f1 }Kmg+r*.J()pBgTBld)Ke|QB1S^mm ^ 3dILap,p| H=g9 xnC>O8:>Z$ iNgcW_< aa=&KO8i3\^!PXI4,e)\Odf|;61!%J j.B'=z?`1eh?>!%%Z_go.BXFW='\&%2088 1' 45-+ ,'#962%$ -  -8? /"$!*,%*,.-7'<-f,'= 1=- '-4HT;.Ju&!;# 8=_oqQaWD.>*5)0&VB$ &(B#,K(E&KSC!)5(;881 087, P*0cGE2\E y! v -..2  "]8-9K';0 $ 0=Y) D5*-$ *$E,%& g#00/,/FHN=.:17F5"-GH)%L-.rd;iBT}^Ds(+/A"(*&P6+)@Q3Dg%().qn1G,!!q6Y9QZ[MA)2+1!&^>NDYQ0G-)4>5J41/!2472n6!00U V wKK>:?1L/FOCQ$):8eb;$<oGGGAGGCGCGCGCGGGGCCAACTATAAGTAGAACTATCACGCTCATATAAATAAAGATTAGAACTAGTCCCAGGTCAAACGGGAGCCTTTGAAGGGAGCTCGCATAACTCTCCTCCCGCATGACACCCCGAGGAGAATAGGGACCATGATAACACGGAGAGCAGTATGAGGTGTCGGGGACATACCCGGTGCGGTATTTTTTTCTTTATATTATCTTAATCGCCGGGCGCAGGACCTGGTGGGGGTGGACCAAGATCTACGTATAGCGGATCGGTCTAGTATTCTCTATTGAGGATGGGAGGACGGCCAGTTTCTCTTCCTGCGCCTAGAGGGAAGGCACCTCGTAATCTATCTCCAGCTTACTCCCTGGTGCTTCCCGAGGGCGAGGGGGGGAGGGAAGCGCCAGTTCTTTCTCCATGGAGGACGAGGGGAATGGTAATTGTTCCATCAAATTTCCATATTCCAGAGGGGAAGGAAAGGGAACATTCCCATGGTTAAAGAGCCTCCCGATGCTAGTTGGGTGGAAAGGAGAGAAGAATAATTTACTTAAATACCAACCCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-31 18:04:18 -04:00AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS6BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA $I~DATA $I~.$DATA $I~wDATA $I~ DCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~4|LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOIS P1AM1b P1RL1baP1WD1b%%P2AM1b-P2BA112KP2RL1b)PBAS11 PBAS11 PCON110PCON11PDMFPDMF PLOC1bPLOC1b;PSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N% SCAN3SMED SMLtSMPLwSPACA{~SPAC SPACA{~SVER4.0SVER 5SVER,,vScal@Scan3TUBED11Tmpr<UserphARphCH phDYbigphQLcphTRphTRV+This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS6BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA $I~DATA $I~.$DATA $I~wDATA $I~ DCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabD`FVocDOFWO_GATCFeat//D GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~4|LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOIS P1AM1b P1RL1baP1WD1b%%P2AM1b-P2BA112KP2RL1b)PBAS11 PBAS11 PCON110PCON11PDMFPDMF PLOC1bPLOC1b;PSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N% SCAN3SMED SMLtSMPLwSPACA{~SPAC SPACA{~SVER4.0SVER 5SVER,,vScal@Scan3TUBED11Tmpr<UserphARphCH phDYbigphQLcphTRphTR&T`XDG7IG!Hgn+1Ab;*55p]?'KzK:*]Q{Y^@>=,8,XNKioh|m C]Z&|k1jK4|<0S1pPRuKojmqy=Y`gP#0beaA({]/wQ2a52rW.X}=1m)h>VO@A$^8c("RNGJr=VUWM9=hO5+znJO!p8Npd-(bcnP6GZ]#(lCKOMQw T"S2Mmn[Ca,k +9}WQ@UzbaI23ttrRXc|LVn!<|'kU$3g;Z6Obl% eIe5_@{&]t[XS_Q7u2&q6X+9mvH%lFGTF4k}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47.'s1Hhl@Ft/9pV|3#+FJl$W+V5ez7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MK>B%P^gWT^Fu*t&:_Gr9#(+gbk1^/.S3xCmqy=Y`gsB0PPOw[h@tC]Q#V>Ax`X~lTmt.qK|-o3o*oUnY!9s!&&v_QjXaM~3>>//x3L#Id4|Ca$"T'MT,Tc%ToN668d#