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GGG@AAZZZooo   !!!((,//565<=<CCCIIIOOOVVU[[[a``ggfllkqqqvvv{zz~~~~~~~~~~~}~}}}}}}}}}}}|}|||||||||||||{|{{{{{{{{{{{{{{{{zz{{z{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{z{{z{{{{z{{z{{{z{{z{{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{||{{{{{{{{{{{{{{{{{|{{{|{{|{{{|{|{|{{{{{{{{{{{{|{{{{{{{{{{{{{|{|{{{{{{{{{{{{{|{{{{{{{{{{{{{{{{{{{{{{|{|{{{|{{{{{{{{{{{{{{{{{{{{{{{{{|{{|{|{{{{{{|{{|{|{|{|{||{{||{||{||{|{{||{|{|||{||||{||||{|{||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||}|||}||}|||}|||}||}}|}}|}}|}}}}|}}}|}}}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~}~}}}~~}}~~~~~}~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4699c7fedad849999efbc77a89274196Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-31_16-33_01053130Seq1281812818_Steier_K1336_trnHjmadera96-WellK1336_D10_trnHKB_3730_POP7_BDTv3.mobSeqPop7z07E[|!Gt>k^79_" o d A V  R P  5 < K K* 3rrYNP]r9_0AJJB4" c0}>kZK=/$"0HftR2zgYOG@93//3:ESdw4Md|.ZDU t<^nc  8 g h>`Vlj h  $i{ w9 g ^ o 8+tI[{R7+*/46413?X;;6= 4 . * T c U - / - ooO^ 1^w1%x4^5 o\K<0'##&,1589:@L`~9l $Npex w:`&\"#B\rnT;$vbK2YV*7#Voa_cjprqnkf`WL?3*')/7@GMRW\`ba^YUSTW\`a^WND;2*"    'TV$tBjN  "5J\imj^N;* ,9DKLG=0# $WkEJ)-}@   "0@NY][RD5%               '5?CA8,      2G[krpeUD5+&%')+,,-06?L[ju||wmbWOJGC?81*%$&*/4:AIRY\XN=+  /CT]\O<% !:RcjeV@),<IPNF9*     -;FG>,'e!}sc* "%#"-9DJKGCDNc~E+L7k\Li$y\a#%KtW) AZSY+mQ$*("  "2CS]_WI6"             "!         !   !       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(:LWZRB.+GelQ7"       .AOTPB/-Mq{Y:#  #9O_d[I2 $?ecC-!$*./+%  4HV[UF4$ 2No~_B,!!(19;7/% (0563+#"1F]oxteN9,0FltP:14;BFD=3'  '-/-' %8Qoy`S[z&MXFyv}gRC95579985/'                                  wdz@,AAW$ASKB.bcpKB_3730_POP7_BDTv3.mobtermGAGTCGCGCTGGCAAATATCAGTCACGGTCTCGTCCGCCCCTAATACTATGTAAAAATAAGCTAAATTACAATTTCTACCCCTTCATTCTTTTTTTTTTTCTTAAAAAAAAAAGAATGGTGATTTACTTCAACATTATTTTATTTATTATATACAAATAAAAAAAAAAAAAAAAAAATAAAATTTTTAAAAATTTTATCTAAATAAAAAAGTGAAAGTAAAGGAGCAATATCAACTCTGTTGATTTTGCTCCTTTACTCTCAAAAGCTCGTATATTTTAAAACAAAAAAATTATCCTTTTTTAGATGGAGCCTCGGCCGCAGCTAGGTCTAGGGGGAAGTTATGAGCATTACGTTCATGCATAACCAGGGAGTCGCGCTGGCAAATATCAGTCACGGTCTCGTCCGCCCCTAATACTATGTAAAAATAAGCTAAATTACAATTTCTACCCCTTCATTCTTTTTTTTTTTCTTAAAAAAAAAAGAATGGTGATTTACTTCAACATTATTTTATTTATTATATACAAATAAAAAAAAAAAAAAAAAAATAAAATTTTTAAAAATTTTATCTAAATAAAAAAGTGAAAGTAAAGGAGCAATATCAACTCTGTTGATTTTGCTCCTTTACTCTCAAAAGCTCGTATATTTTAAAACAAAAAAATTATCCTTTTTTAGATGGAGCCTCGGCCGCAGCTAGGTCTAGGGGGAAGTTATGAGCATTACGTTCATGCATAACCAGG 2KXbw%9?GWby "*3BMT`ir~ %/:DP]fq{ $-8EP]iv "-8CNYdq} %1;FR]jw)5@LWco|  ,7CNZeq|  $ 0 < G S _ l w    + 7 B N Y e r   % 3 > K W c o z  " . : F Q ] h t    ' 4 ? K V b n z  "-8EQ]jv  ,9DQ]ju+7CO[gs(4AO\ 2KXbw%9?GWby "*3BMT`ir~ %/:DP]fq{ $-8EP]iv "-8CNYdq} %1;FR]jw)5@LWco|  ,7CNZeq|  $ 0 < G S _ l w    + 7 B N Y e r   % 3 > K W c o z  " . : F Q ] h t    ' 4 ? K V b n z  "-8EQ]jv  ,9DQ]ju+7CO[gs(4AO\   "8%  *,;>'".2+2.;>1;;;>1;;";>>>>;>>>>6+"'"(#6;22888;;3311333>>>>231;1+62+0$.;;3120,"".+81222;.88;3+8+18382;+;88;>8>>>>>6616611  """"""" "   "  %" "%  "     "$"      "    "8%  *,;>'".2+2.;>1;;;>1;;";>>>>;>>>>6+"'"(#6;22888;;3311333>>>>231;1+62+0$.;;3120,"".+81222;.88;3+8+18382;+;88;>8>>>>>6616611  """"""" "   "  %" "%  "     "$"      " #Y)bXM?DNc H*H9%BTM9VUEY)I#16 S S-KEZZP#IR)< M#U8 F>!:G]6#L)"F-W4J#H)VR5DU< K &)9Y%=0%"1)V1*FMN\-B8 8 OOF1 #698P*L\E%AU%]3 AM)[[a6BM8 [@'4=GJa]VF/;]NYC-6\>^SNID'O"XB4?GS J+MV  O8<0K[ _:)^EH>PO2 40U +^'%EI$'[ =YEU&>WQCD$4]& R7VMa8`Y#1:G$7ZT8IC]\;.H F4$ ERUPk |#;`dS2&/p7'gI:Bi_KTL8p#WGL!K_2Z!LiO1lMYvpjer |.ga,dx|~ "x;sT$TZ#'$/>$~mk+wVq$r.=M/b 6Tc!Tp=45qF5&Y4a8Eu=MDn?9s8_sax7Lvy^zi}J g; 00Qf[$g y!.(h$cur 6  P H  U#Z <f-g @B3pNNI`WCO{DlVp.@03{"!K`  *        5 )8'pppFp9l8pd%yJ=d}L rkG{4?,nYZesqB-P%[dG#pLdCP.mgD,'{^>p93v9K8&sahO<|O.0A@`;?o/?(EVKTY?A3d%hI zn&:JVdtXxt>=E\$p^UeT9/s(F@"+V wc_Mt/Q>\xBaARc$: 6n|Kzk0)X( ;C4  xe ,3*1;H'`0=# 9ON,-2p4X 4!D:6FC"n5ujCVvW&,R`oR;% )0S*HCDe`(B\}"U|eby'u /!s7-2<-ly4Q0;8!,70)!9Y.L\WE/5/K = DKONEIgV?"vx@{Gg:vt.-g@HR[?{dLEV")T6ki),cAKu'fP"9ER<_0 f`jJ@ 34aAGCGGCGCGCCTGGGCCCAGTTCATTTAAAAAAAAACGAGGATTACAATATATGTGGATGAAATTTAATACCCCAACCTATACAACCCTAAGAGAGAAACGGAGCCTTTTCCGAGGGATGATAAACTCCTCCATAATACCATCCCTCATATACGCTTATGTTTTGGGGGGTTTTTTTATTCCCCCAATTTTTAAAATCTATTTATGGGGGTGAGGGTATGGAAGCACTATCACCTCTGTGGATAAGGCTCTTAAACTCTCACCTGCTCGTATATAAAAGGGCATTTGGGTAATCTAACAAAAGATGTAGCTTCGCCGGCGGCTAGATCTAGAAAAAGGTGATGAGCATAACGTCCTTGCATACCAAGAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-31 18:04:06 -04:00AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS/BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA %KzDATA %Kz0 DATA %Kz{DATA %KzDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEJLAST~~.,LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISP1AMq!3P1RLqQP1WDq$P2AMq)P2BAqq,P2RLq&PBASqq:PBASqqPCONqqPCONqqoPDMFPDMFPLOCqPLOCqPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%SCAN3SMED SMLtSMPLwSPACAx`SPACSPACAx`SVER4.0SVER /SVER,,vScal@Scan3TUBED10Tmpr<UserphARphCHphDYbigphQLcphTRphTR(This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS/BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA %KzDATA %Kz0 DATA %Kz{DATA %KzDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab>FVoc=FWO_GATCFeat..=GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEJLAST~~.,LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISP1AMq!3P1RLqQP1WDq$P2AMq)P2BAqq,P2RLq&PBASqq:PBASqqPCONqqPCONqqoPDMFPDMFPLOCqPLOCqPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%SCAN3SMED SMLtSMPLwSPACAx`SPACSPACAx`SVER4.0SVER /SVER,,vScal@Scan3TUBED10Tmpr<UserphARphCHphDYbigphQLcphTRphTRRz0L/kbnE!0z^n3HVQQEO2astcZ~~VGC2lB)lW+Uf066}ZC__cIBM}.z zFef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`P|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTveurDmCq?`DQ`+}{Uplql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W@Vk1$#=4ZbZ4yM=VrTH&zqrua*b iq*#7rO/adu{>(Jt6qZ><}P?[NB@vulc*{w@p;#(":?S`,j.uFTcM#yKA#2rBA++muIl4Nf)C^DZ=<7:cmj^k-T0M5[S*.JpxcSX`"lef5U_9}|Ate1}1"/bW>y&bC]LVn!<|'k0|3.B+0Oj!Q4X+*flaO8JB6E$} {r*i`?e Ml1}ssb[l"G>=?]p&k6v)ukO"yA&d}8qQgE5]U'j*-[~!Tq*nEg8Z~KI'_26?^V$Yeaejp~{>ZHm? >#veu]@|Z5hf6]X?K90#qN8>o XS_xP=O_l;mq;;9VgO(_y"`k:!.tkSY4E2 (#>+6hjbzpTZy:'Gkg_!#=Kr)YW [xinJil]ZCd]'DZNu@v0kc "V&S$~=}F)h>;-T)RJTZmU)P? sIe]>BZuGZ-p@vulc*{<=pjHOo&eG1Vcy[*oCf`y6n?2x`agv`kt3G/3t\#9/hV6T]==6X12O[n/bsDU5hf61FA>&oPWTRI1]f)yA@r9s_|g+*F}t.("P~fI2P&N+E/Gz^n4.6t}VX*os7}Z&[Eb4;,}s@dUa0u:W^$B!X`7.ykI0o6Gzv&1fu4WyYZFU5FS7&3-OT5ro{`N%7`h?&03ghW~^(';_/C]Q#V>Ax^c~=$~~ !T_*|QK:jin'D5?C7B.V[ZAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtAUDTllL"B1PtB1PtBCTS/BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA %KzDATA %Kz0 DATA %Kz{DATA %KzDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab>FVoc=FWO_GATCFeat..=GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEJLAST~~.,LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISP1AMq!3P1RLqQP1WDq$P2AMq)P2BAqq,P2RLq&PBASqq:PBASqqPCONqqPCONqqoPDMFPDMFPLOCqPLOCqPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%SCAN3SMED SMLtSMPLwSPACAx`SPACSPACAx`SVER4.0SVER /SVER,,vScal@Scan3TUBED10Tmpr<UserphARphCHphDYbigphQLcphTRphTR