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GGG@AAZZZooo   !!!((,//565<=<CCCIIIOOOVVU[[[a``ggfllkqqqvvv{zz~~~~~~~~~~~}~}}}}}}}}}}}|}|||||||||||||{|{{{{{{{{{{{{{{{{zz{{z{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{z{{z{{{{z{{z{{{z{{z{{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{||{{{{{{{{{{{{{{{{{|{{{|{{|{{{|{|{|{{{{{{{{{{{{|{{{{{{{{{{{{{|{|{{{{{{{{{{{{{|{{{{{{{{{{{{{{{{{{{{{{|{|{{{|{{{{{{{{{{{{{{{{{{{{{{{{{|{{|{|{{{{{{|{{|{|{|{|{||{{||{||{||{|{{||{|{|||{||||{||||{|{||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||}|||}||}|||}|||}||}}|}}|}}|}}}}|}}}|}}}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~}~}}}~~}}~~~~~}~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6415f1bb7f944847b68eb8cc38b29ceaSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-31_16-33_01053130Seq1281812818_Steier_K1336_trnHjmadera96-Well K1336_C5_trnHKB_3730_POP7_BDTv3.mobSeqPop7z Ffy)Ru]1j QL?W  c 0 [ s z t h [ K 5  5\2i p*Ntqpnjiq-Ts_1jK)rhcdm| *EYdiijoyfDw[B+>fkNC n  R m Z  O S*+<E  %3R]j : w KEbe zN%0mG7-prD v%IvH 0[7OYQ:u> zN'lUA.N?/>dA205<>8(J L #1@Qbs~rkjp{zeK- /NNh[)w Bpedkw}m]OC:4/--.03454446:AIQY`ddb]ULA6+!       %4H_vv[C1)+4ANV[^cqO>`\0t@"    (19AGIG@3$   $DjmG(  1p*1l1 !.=MY^YM:&&?Yrv^B(  )9FOSPG;,  ,597/% 5s-r _.%4?DC<2'   .o> <94k, !$).7BP^ipqk`SF<521344/''KN-#"f,          6\rN/   (l:'h,   '}p ghmd#6:cpz.  ,:DHD9*   %rnRuQnv*  8Skz{oV83OertiV=$   +@Sae_Q?,         0AMPI:( %4>@9,   :B'Oe k]o_      3FWbc]PA1! <%`X_A*0>E=+ -Sg <`}x]@'  4RnkN0 #=XmwsbI-     !(+(  *362(  V~s O #*-,'    ML].0i& ZQ  > 1+u2     <l+\  /@IH=+ JQ% #:Qaf]J3 ';KSOB/  0CPSL<*  0k@YIcR)K9 2JZ_[N<)  "$%&&%$##$%%! 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"$$"                     z@/A yAIAKB.bcpKB_3730_POP7_BDTv3.mobtermGATGGTCGCCGGCATGCGAAATGCAGTTTTGGCTACATCCGCCCTATCCTATCTTTAGACTATATTAAATAGATTCTCCATTTCTTTTAGTAGTTTAGTACCTTTATTAGTTTTGGATTTTTGAATTTATCTTAAAAAACACACACACAAAACGGAATTTTGATGATTTTATTCTTCTAAAAAATACTACTAATTTAAAAGACAATAAAGGAGCAACAAACCCTTTGATCTTCGATCATAAGGATTTGTTGCTCCTTTATTGTCTTTTATTAATATTACATTTTTTCTCTATTCTATTTTCTGCTATACACTAAGAACAAGTCTTATCCATTTGTTGGAGCTTCAACAGCAGCTAAGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACCAAAGATGGTCGCCGGCATGCGAAATGCAGTTTTGGCTACATCCGCCCTATCCTATCTTTAGACTATATTAAATAGATTCTCCATTTCTTTTAGTAGTTTAGTACCTTTATTAGTTTTGGATTTTTGAATTTATCTTAAAAAACACACACACAAAACGGAATTTTGATGATTTTATTCTTCTAAAAAATACTACTAATTTAAAAGACAATAAAGGAGCAACAAACCCTTTGATCTTCGATCATAAGGATTTGTTGCTCCTTTATTGTCTTTTATTAATATTACATTTTTTCTCTATTCTATTTTCTGCTATACACTAAGAACAAGTCTTATCCATTTGTTGGAGCTTCAACAGCAGCTAAGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACCAAA05ITbgt&;DU^t'0@HT_iv~(1<GR]kx*3?LVbnz,8EP\iv&3@KVamx'4ANZdq} &3@KXcnz   ! - 8 C O [ h t ~    % 1 < I U a m {  " . 9 E R ^ k u    * 6 C N Z g s   " / < H U a m z  !/:FQ]ht*6CN[gq~)5ALXeq}+7CNZfq|'4AMYep}05ITbgt&;DU^t'0@HT_iv~(1<GR]kx*3?LVbnz,8EP\iv&3@KVamx'4ANZdq} &3@KXcnz   ! - 8 C O [ h t ~    % 1 < I U a m {  " . 9 E R ^ k u    * 6 C N Z g s   " / < H U a m z  !/:FQ]ht*6CN[gq~)5ALXeq}+7CNZfq|'4AMYep}    #826%8333!*! **3+)"-3;8;,8;8;;;>>;;;;;..'11% "1+(21000$2311+8;08;8;;;8323;;;;33;;81;288.2.13+28;3;+;26.33;2.>;;1*.;>.8;38;88068.13383;>>>;>>;>>>>>;;882>3383;88;6683;331.83;;;822;3;22."2131313+21+22>;;0'#$"";;;;;>8;8;;+++'++"(1'"(+382838''))"1'+)3&+.''1"+&')++-+'2283-"'+'+&"-+''')"""# *33''&&#,%*8>88'&# *&'*&''*3     #826%8333!*! **3+)"-3;8;,8;8;;;>>;;;;;..'11% "1+(21000$2311+8;08;8;;;8323;;;;33;;81;288.2.13+28;3;+;26.33;2.>;;1*.;>.8;38;88068.13383;>>>;>>;>>>>>;;882>3383;88;6683;331.83;;;822;3;22."2131313+21+22>;;0'#$"";;;;;>8;8;;+++'++"(1'"(+382838''))"1'+)3&+.''1"+&')++-+'2283-"'+'+&"-+''')"""# *33''&&#,%*8>88'&# *&'*&''*3 d!5[5JO"K49. % O"G,PTVGD:!?$[2dF @<6P3.XFDA>% KW ?,,QKbU2B* G5$=!6F,>;?<MG;7]45dY W^bE_HYUbK8S=$@5C)`?ZS );Y4\/ c'8cB. 9#Jb-EM3. \c5 I'  -)JZ>+T2-!I C<"YBDd<W-0Q-W/(@ MaB;5JJO7<OY!2S J11R* 2VU8.G"`1=-`Q)Y=L_cBW7I4TP/+#4CWA ]GOIWN ,HT:CH1Y3(V;B7MGN[GE]-<=WCS:#"G4U/'D`NU]H7 *bc3N` LC/S>P (*Y:#. R/?2% 1 4L`xM `r4)0TLji1T_hC?KMa"D9Y8NwL7?;{}/AU:6,rv/.?<vl9&^&'z:gb%l0xNcE0y 2f<M^$ZNc&oh Iq1#c@DsZ[k~;?c~ f#Kd!DbNgQ){hTE0o$;t`{y){5QWh}v;ZsyCX8g&!2}sg:q\j  H  `{  ~<"~< 1LN%q[hzyOkR3WL?[:h7EQ3,SoF19'1       #H  %"?>:  &H  !"R?|pT+u,z< ywd3~7,2KY&drmW8dp5?E#14#;:"riLXSHHB ?t</`o(;wZ}i 8*,,@ch66 zyH pzO&;*uX.,;^wB s+)xmF/V9u/<z1Ug]CB,p*dpq43EX HgaF9@2J:p4l|SV{@N}j6EC<yrf;'4/FIAGGCCGGCGGCCGTGAGAGGCCAATTCACGTCTACATATGCGAAATCTAATATAGATACAATATAAGGTACTTAGGGAACGCAGAAACCCATAAAGGAACAAAAGGAATAACAATATAAGACATTAAAGGACCTCCGGCTCTCTCTCATCGCAAATCAAAGAGGTTGAGGCACTCCTATTGTCTATTACACTTGCAGTGTTTTTTAGGGAAGTACCATCCTGAAAATTCAGCTTTGTGAGTCTGGGGTGGAAGAAAAATACAAAAACCCAATTCTAAGACAGGGACTAAACAATACAAAGAAATGGAGACTCTTTTTAGTAAAGGCAAATGAAAGGAAGAGGGGGGATCTGAACCGAGGGTTCAACCGAGGGGATTGTGATTCCCTTGAAGCGGAACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-31 18:03:42 -04:00AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS6BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA &SLDATA &SL1KDATA &SL}DATA &SLʗDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE+LAST~~5LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISEP1AM'P1RL#P1WD*,P2AM0hP2BA3P2RL-JPBASPBASxPCON"aPCON PDMFPDMF\PLOCPLOCPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%=SCAN3SMED SMLtSMPLwSPACAyG#SPACUSPACAyG#SVER4.0SVER 6SVER,,vScal@Scan3TUBEC5Tmpr<UserphARphCHsphDYbigphQLcphTRphTR'This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS6BufT#F1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA &SLDATA &SL1KDATA &SL}DATA &SLʗDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabDFVocDFWO_GATCFeat//DGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE+LAST~~5LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISEP1AM'P1RL#P1WD*,P2AM0hP2BA3P2RL-JPBASPBASxPCON"aPCON PDMFPDMF\PLOCPLOCPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT !RUNT :RUNT 6RUNT 9Rate RunN""S/N%=SCAN3SMED SMLtSMPLwSPACAyG#SPACUSPACAyG#SVER4.0SVER 6SVER,,vScal@Scan3TUBEC5Tmpr<UserphARphCHsphDYbigphQLcphTRphTR]nd3ghlc T-* +Wt3z{0bInTor@tbfD7E)0Ie/AK8N'nsv&'Y?sxWlZN.YRz!#fn2%FT!sz^2h3)-I5d~)Uk}`:r&xvr_WG\|8 Xo2h{/S9~i,EAx8amb#QJU0iHAj}W2yv!a!<}B]rQHfKbVU}Uz1p=|(LZjp7xMO9j;q6TB(2JLvuUlN1@#vQ!TMF4FprjDp*K0.]+0Oj!Q4aK{WlVO:}mldvbNhsrU5-uvCbbJ_<$AJu"#p|`*d,SEu1Y$I`Mc_0f4[42g|5[S*.J-omMOxkYEZ&dAro XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]wnF){xD\4AFU?-4$&#S%TwLhl69eD>Ii#9cy|URV T2|rml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_|owoqiY.] z,&0@1>X`]yVlX/-f>P5CRV1]cK=i~E&Xu4FsO1X YhNW~L?n[Y<,h`\wmj7a;A{%i6#a)lmeudrVLgJRv)1mfc4hApvaUG