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, ' ' % 2 $ $ ' 5 4 5 & , & & , " 0 $ ! 1 - & + 0 + 1 3 % + , 0 $ ! + 4 , , % 2 8 0 1 < 2 9 F / O ? 8 ? = 9 ) 8 0 ' " ! ( " ' - # ! , 5 * & # % 1 ) + % - & , ! ( ? + . / : 8 6 ; - 7 6 . % ) ; B ; D - 8 6 4 A > * 1 . ? 7 , > C < ? ( + 5 $ . ( - ) # . 4 ) ' - : , + ( , 4 # & * 7 4 $ * 9 $ , 2 # " / 0 / ; + & * 4 2 3 . / 5 ) . & $ 3 . " , C & # C E % ( 3 < ? G U Z \ t { ` W G : D 4 K > 5 A A < D ) > + ' / 7 * , / : - ' , , + $ ( " * ( , ? B + # > U T a u q ` c X D @ - / , . . 1 3 ) , * 3 $ * B 8 B P @ 8 3 7 1 & $ 8 0 7 @ & + N : / A 2 / ; = A W \ ~ j e ` > F 1 A + + ) - ? $ ( 2 # * $ $ " 1 ( 7 : > C > : 5 & 9 I A Q k i m b \ = G 9 G ; 4 . * + = + - % ' ( / 3 8 4 5 , < & 4 = 6 5 , % + % & / ) # ( * 5 4 @ 0 / 6 & I C B + U W m y } d H J V ) 1 4 J & ' 5 , 9 8 ( ) < 2 C 0 ; ? $ ' ) ' ) - ! & 9 ; # " ' # ) 1 ! 2 ' 7 1 0 ' 9 / A F E C B : 1 < - + 1 ; 5 - ? ? > = < = = = > E + 7 2 0 < 6 # ( ! # ( 0 , $ - 8 0 % 4 < > * " 4 * ! / - 7 / ) : , . ? / B 6 8 R ; 3 ; 8 4 - 4 $ . + 5 0 + 1 3 4 : & 4 0 @ - 3 3 4 @ % @ 3 * 5 3 , 6 2 8 0 2 = 7 [ O L W Y a T P D C Z I \ u n } N F T N I ? K 9 E 1 ; , 1 2 ? + 4 @ F I 3 2 3 < 9 * 0 + 0 3 0 J > O \ ] w v N f f R D = A = A 3 7 < 2 < < 7 , / . 5 2 : T 3 / ) / , / + & , 5 / 5 ' * 4 . ' 0 " 0 2 ( ( 5 / 4 1 = 5 3 / " 0 8 7 2 5 - " , 6 7 : : 6 D % 2 ? N C ^ q Z r P b ^ G > ? 9 7 0 * - 3 4 $ # = / ) 2 ) - 1 2 ; % " . 4 $ 8 . " 3 5 # 3 " 8 , . / , 2 1 / ) 3 ) 3 , 0 E : J 3 1 - 0 3 6 9 C B B 5 = / 6 B 9 5 & 9 F > . . 4 4 : 0 & ( + 7 & 9 + 5 - # - 1 ; & ! % - 7 2 / 2 3 5 0 ? 0 , ' , F 9 9 2 C 8 H Q Y [ z v o F P Y N : = 7 C . D D A : : H O L R k k | n t n V M K D K F - ? D > 5 / 2 . : 8 / 2 3 @ C N F K B ^ E a @ M - > > 7 9 D Y E 6 J H ; H < 8 ? 1 : 6 J ; @ 7 9 E 5 0 ? = 7 6 ; 1 7 6 , + , 3 3 , & G 0 5 6 7 ' / 2 , ) I A @ 4 0 7 1 : 6 : 7 E 8 3 B ? & < < < 3 2 8 = L C E F I P E B 9 F O G I - 3 ; D 8 J Q E 7 / R 4 G I B F H W G D H 9 ; 8 R E F @ 4 7 , % . 7 + / B 6 @ ' @ @ 8 @ D M M Q [ ^ k t z | J N H O N H 3 H 0 B , 6 G 7 D @ 2 : 3 E 7 9 ; B I = 8 : > C N = E @ S B N H L U W T @ X V Y F T + M J ' : , ' / . , 8 9 ; 0 " 9 ' 2 9 5 % * A @ L ; D E W a n y { s _ t _ N O D = > 2 K 2 8 1 8 / 7 9 ? 8 0 $ ; / G ; 8 6 3 6 6 - 1 * / < : , 3 K ( ; < C C ) * + 9 9 1 ; 9 > I L 2 N B ; 6 6 > J 3 M X f v f Y Z N G J K H K / < ; 2 5 # ) 5 3 7 C > & 6 4 . ( 4 ' 1 B 7 = C @ 4 " 0 7 ' : 8 2 + 3 1 6 1 A . 4 / < > 0 N 1 6 ( . R 6 . 4 8 ) 7 B ) 7 / 5 2 6 ; 1 2 ' D 4 : 1 3 > > 6 5 4 8 6 1 & ! 9 ; + ; 1 4 H 4 3 B 6 X M @ 7 @ @ 8 = A 2 O ; * 9 1 @ C 9 5 B B : J E T S 7 : 6 A H G , B N \ R @ D > F I : @ 1 D J = B O 4 G D ) < J A 0 S H F F L G X T P P W N % E ? A 7 8 : 3 B ; I F : 5 K C K J N E 6 > H < 7 E I L A > > 4 T O A E Y h B @ ? U X A H A Z I : 0 - 6 N , H Y I > B L D K V > L K A F G X J P X > G S J : C C = @ 2 ; , 2 - > 5 ' ! 9 : M 9 J > : > I : J J @ L L G g g y k ^ \ X A E C G G 6 8 A A T P 9 K @ G Q B 8 U C Q J D 6 - ? D / 5 1 - 9 * G 2 > J 4 ) & 7 9 0 , @ / @ 7 5 6 ( $ $ ' 3 5 * 9 1 > " ( 7 > ! - = - ( B 1 5 9 6 4 / 7 $ * 7 1 * - . , # . / M , & 5 * 6 8 A = 6 F ? D A > ? 5 G J L Z y w z | j d Z = F 6 I 7 0 7 5 R K > 4 1 9 2 * = 6 > 5 4 @ L A * K ? K G 8 B 5 < 3 / = 9 4 5 9 4 7 ( 9 ' ! ( 0 0 4 ! 1 C * 2 1 0 - ) / * / " / , 2 4 %
2 1 % 4 5 ; ( 1 C + # 6 0 * . ) 8 B 1 - - 8 ; + C 0 2 < 0 1 E ) + 3 0 3 = . * % ( A * ( - ; 2 ' ) ) - ; 3 : 8 2 6 9 @ ( 2 F ) 6 9 D - 8 0 ( * & ( . 7 0 < - 2 G 7 ( D ; , 1 + / 3 - / 4 = ) 4 ' 9 2 ) 3 ; 7 : 9 * 4 9 1 # " 7 # 9 6 ; 1 / * 3 6 3 % ) . ( " - ) 8 @ ; ! 2 0 : 6 8 " 0 6 7 $ H 2 ! / C * = * 0 C @ D < 5 ; : - / ( % - 1 @ E 6 D I = F Z B D / C b f g q v | r R j N O I B - A : - 4 . 7 0 6 , : 1 G : @ R 2 : T C Z T W T B V a _ \ b v u y V S D H : E 9 ? < ; = 9 * 1 I @ G > 8 / 6 = ? T H G B U c _ P v b d [ { d s u x k Y Q 3 7 < F ) 2 F 2 5 B 3 * + , 0 1 / ) C 4 A 7 A < B 7 . E B 7 - 3 H B * B C : O Z I U M = Y E Y \ g q { } p k q x _ P V I I 1 D 8 0 3 A 5 8 , 2 G . ; 7 2 @ H : 0 ? / : 3 : 0 0 # 6 ( # ; 7 / L 8 : , 2 0 + 2 6 > C 7 G T u [ y t r e R ^ M P I = 0 < ; > 6 2 ; C R 6 8 ' $ < " A 3 ) - + # . $ 1 ? D 0 * ; ; ' . = ? C H J , K U V M g m g W \ M L ? ; J B ? ) 3 6 0 C A 7 ? ? 4 ? ) 9 * 5 . C B , - 0 8 8 ) > 2 2 ; - ? / $ J J 6 > A 8 3 A A < R R _ u y d p u } j v m m s ` o w | { y e i v ] U S N F N F K B S I ; D G B U O l c r g u p m [ m j | l w s d ` I b A D ? M ( @ < @ 8 4 7 3 3 F 8 F A G K J ] [ b s u a q e n b f X O G 9 = > 5 I 4 5 ? N 6 F H @ G S S N Y ; i | l z { i e b T P 7 J 7 / / , 4 ! D 5 ? 5 < / $ 9 7 0 2 ' 3 1 % % F 9 . . * ? # 0 < 4 I ) < 7 < 7 I H ; 9 7 7 J F B [ [ r o v S k T Q ^ P 6 ? > / : . 3 7 3 / & 0 B 4 5 6 5 8 D @ / B @ 6 ; @ ? A < 0 2 ) : + 2 1 ? 1 : @ + 7 = > ; A H < W e p y x e b W V B 8 I - < 0 . ; ; = E A ; ' 0 8 B E 6 D E D H > D 9 L Q T Z ] f y z k o e H @ B @ G ? C Q A < - 1 9 3 L M S R X i u l v x k g a S L U ? ; 7 B 9 0 ? = 8 ) I + 6 @ + < < * 1 > 3 = > F 6 . ( - 3 4 , " A . 3 - 8 8 + 9 5 2 B ) 3 . * 3 ) # $ , + 9 * 3 1 1 6 " : ) . 1 ( & ; ; G 1 4 : 3 ' " ; 4 0 3 9 1 6 5 $ / 6 1 2 H < L R Y C X R c l f d ^ N L H S H C ) Q G 8 K A B 6 C / K O I L p h b n { y z e i Z l T _ l | w x h e \ S X < H = 8 9 4 9 G 8 E ( 7 / ! 4 6 * / 1 0 0 : 7 1 = / 8 * E 9 @ 8 : 8 . A 6 > 2 < 5 G K H U K P Y e { t ~ { v ^ r k v t m W x q c _ V n I S P R C ? 5 8 D D 2 E C d ` e s | } r t u x u } r r `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
9 & 1 5 4 . D = 5 7 8 % : : 9 6 6 = @ Z J G \ m k x | | q t b T g m _ z s o r w n v f h c Y , Y > G 1 6 9 9 3 4 + 1 & * % ' . $ 3 + 5 2 3 / 5 * 6 * 5 # - 2 - 9 ; - & , - , , : ? E > = ; @ > 6 8 K : + - , 8 ( 7 > ? 0 = + ) - 8 I Z Y c Z ] b e e Z R E R C 3 8 1 8 : $ = * - * 1 * + * * 2 0 * / 5 = / < 0 & ) 1 8 3 ) . # ( / # 5 1 - 1 / 7 ! / - ' ( ' ) : . ) ! ' " # < , - ' $ 2 # : / * 8 0 $ + # & , 0 3 ( ) 4 ' , 0 - # & $ / " & 6 ( 0 6 1 6 7 7 > < S H F M X e a j h [ m m Z e _ _ p V A M J % / 9 0 5 9 0 + = 4 - + ' 0 . & , ? ! 7 & B & 6 ) ; Q T N M C X c k e i r Y I O Z C < = 6 6 A / F & 7 2 / . @ , 0 5 0 / 3 $ 4 4 4 C : 1 3 7 B 1 9 > E 7 % 5 + 0 $ 7 5 ( 8 ! 2 : + , / 6 6 < ? : : : 1 ? L H O V N s c f q n u d c W W X U ] [ b ` c q t x { \ l Z Y N O P E 2 / 8 0 2 0 2 , * - 5 9 > ; ; 0 4 @ = 9 3 C $ > E < I B ) / / 3 4 6 , + * 3 5 3 3 / @ @ 9 \ E R X R V h m ` a [ P I O D D > E 4 ) : < ) ; @ > A 8 + ; ) 4 6 % 0 . B 0 & 2 ) - ) - / 8 0 / , 4 0 = $ ' * / ( * & . < & * 2 / 4 & 0 & A ' . 0 8 . 3 " $ ) M 4 9 3 ) 2 5 9 - % % ) , 5 ; . " % % / 2 2 " ! - + . . 0 ) * ( 7 5 * - 4 1 & 0 8 0 8 ; = . 4 E / E < 8 3 0 K K = B K R e p o [ s { s x u o y o h U e k ^ s { w r x ] o i X L C < 5 / Q 2 I I @ * + + ( 7 9 * - 0 ; * - - $ ( ' 2 0 + - , 0 $ ( , ' ) ' $ ( * , $ B , 4 ) ' 9 + ) # 3 * $ ( , ) 1 % % # % ! * - 8 < . ' 3 : % # % - * - . 3 7 * G . 1 - 4 3 < ) " + ! 1 ' 9 3 % 1 1 7 0 - * / % 0 4 4 . / & $ + , , . $ + " 7 ) ! % * ) 0 3 ' % E " ( / 3 = 1 > / % & / , 5 % ( 3 4 . % ; ' " 1 & ) ' 1 & ) ( 5 ! $ " 5 . " * " % 7 ' ) ? ? , 2 / ; 6 0 ) 3 / : ( 9 / 0 . A ? " D - D H < < O S W _ ^ ` [ s a s W S M N O E ? D C 2 2 C 8 < < ; K 4 L - - > ! = : 6 0 ' / I ' = O E K A \ c v a x | k t U \ _ e M K Q S X Q C A 2 E I B E ; L O C E P H 6 B J > 9 M ; B O D b _ W k s h t w a q P P R : M a K [ 9 Q ; B 1 C , * 3 2 < 9 ? 6 ? = G * O A E 6 > : 5 5 ; ( 8 7 H E 5 9 A ; 0 2 4 A 4 1 5 0 9 9 # 7 7 C 6 1 . + = 6 6 < / & 3 ( 0 = U L C W H T I T a _ _ d V K Q N L T H R G _ ^ ^ m f s o h l S d _ K _ X 3 . 2 D 5 , 3 < ) 1 9 * 2 / + 5 D ? 7 / ' . 0 3 3 $ % 1 9 - , ! 1 * ! * * . 7 2 " ( % ' 3 2 ' , # 8 = 9 1 0 5 ; K T V [ p d t _ _ \ g O _ E I G : . @ E ) < 1 & % " ) * " - ) * ! & ) * % ) & ( ) / 5 % 4 # H . + ! ; # 6 4 . 1 8 > > < . , 5 6 7 ' ( ) * . , ) 2 . * ' % 8 , % # 8 ( $ " 5 9 - 0 3 ( = 3 = . 4 ) 7 " - $ 2 3 D , - # + " , 3 & ! 5 % ) * $ ! 7 ' < - ' 0
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0bcce605854648a1845743587ed1e869Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
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00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_16-33_0105 3130Seq 12818 12818_Steier_K1336_trnH jmadera 96-Well K1336_C11_trnHKB_3730_POP7_BDTv3.mobSeqPop7z , . 0 4 9 @ F J L O S X ` g o w +5@L[jxwi[M?1 { t m f ` [ X U S Q Q P Q R U Y ] b g m r w | ~ { w t p k e _ X Q L G E C B @ ? > < < < > @ C F G F E B @ ? ? @ B D E E D C A @ A B E H K N P S V [ a j t
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KB 1.4.1.82018-07-31 18:04:20 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 2 BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA $q H DATA
$q H - DATA $q H vj DATA $q H L DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE [ LAST ~ ~ 0} LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS 6 P1AM 8 P1RL 8 I P1WD 8 ! P2AM 8 *) P2BA .a P2RL 8 % PBAS
PBAS i PCON - PCON PDMF PDMF M PLOC 8 PLOC 8 PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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RUNT ! RUNT : RUNT 6 RUNT 9 Rate RunN " " S/N% . SCAN 3 SMED
SMLt SMPL w SPAC A~J SPAC F SPAC A~J SVER 4.0 SVER 1 SVER , , v Scal @ Scan 3 TUBE C11 Tmpr < User phAR phCH d phDY big phQL c phTR phTR 4 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 2 BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA $q H DATA
$q H - DATA $q H vj DATA $q H L DCHT DSam DySN
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RUNT ! RUNT : RUNT 6 RUNT 9 Rate RunN " " S/N% . SCAN 3 SMED
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