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W L * ? Z G M 9 Q R U G @ L D I K F N 2 8 W ^ ? F _ L > T C ? ` ? J F S L M 3 > K D 2 ; A K Z T ^ J ; K = Q Q J B ; . C - B < ; C e 3 3 H B A 6 E 3 @ 2 I Q J E 4 H F < L E = 4 ? < Z @ 1 4 6 E > D 4 > C K 0 7 L B J 6 % : R ? = D F J E N A R S _ O J P 3 > P 1 D T W W U K X G X m D O R K C V F U ; N [ R ; < J K : > ? ? M E ` O c S d V 9 Q E Q > 7 Q V a G ` R E f G ] O 0 6 Y G ] Q K [ I F J 4 a M Z > > B @ E J : P T B < + = F 3 G < C Q Q \ L C R K > ; Y R ; V F : 0 K S M - F H / F P B O Z W Q H J ^ K / E = L F : : E R K R 6 V T I > N E O S A B 7 A S ? 9 B 9 ? G G I J K G c \ F R S ; T > 3 ? G 7 \ = H @ H 4 Q P 9 I 9 = M = = < 3 ! L 8 : 0 ? M T ; M C 2 D > 2 - I . > > , 7 S H C B = ? E C M S H U E G 8 F P C @ W G 7 < h O : K J 9 > I D 6 ? @ / R I - - > ? F O J J 5 A L 2 V ` 6 5 K G A J G > X U F ? 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f 2 G = : B @ ? A = B ; 7 , ? ) K 3 A 3 B 7 E + ? A , 4 5 C T A = ? @ 1 6 ( > ) . @ ' 0 3 H K 4 ; D G - J E M H X ; F 9 G 9 4 + 8 @ 6 Q : D > E ; Q 2 / < C @ A R = 4 F E H / : A : ; I ? 5 ' & A P F H < 7 7 B > L ( L 0 G ( 9 E T J 6 C [ D B 2 ( 7 8 G R J - C F H @ < D B I 7 : 4 . 8 7 C D , 8 I G , ( : N D G 5 C J D A 9 & 8 + ; : 6 I : B I [ J J R F : = I a 5 ; = I F = I = 6 F B G T 4 < E ; > : 7 A C D 6 A J E 5 ? = F . L / 9 7 B - 2 < K ! A B G 6 5 , < F H 2 B 4 L K 3 Y C 6 P 2 D < X 5 B = 9 R [ S ? O 0 F D M 1 F C C : , ) < E : C > < . 1 N = K , N J 4 J F 7 < @ : 7 7 R = / R L ? P 6 / E B G E 5 8 @ C D M + B ? T E P 8 R % O ? Q K L = ; H ; ? N F < 4 : % 9 = G ; 1 F A U 2 6 @ I 6 9 G + F 9 4 B B < 6 5 8 ; $ F U 2 * < G 8 L ? H 5 : H / D H / T = & @ ` 3 U E R ( ? * ? D N 6 F N H ? 5 6 P 2 2 8 2 Z M @ H = 8 ) ? H G 4 B > < : G J F A 8 K 9 @ H P 7 1 : @ C 2 A < < P R D F I : < : 9 R = A Q G . * 2 = 7 A = A < < S A 8 9 W * < 2 + A / 6 9 D D 9 6 N 6 J B ) ; ; E G : Q a F < 6 9 + 3 M Q : c # @ ' : 8 E D L < ; E @ E C 3 C : 2 K A C B M " : C . 0 ) @ & < B 4 B G Q B 6 M 4 : = D B @ 6 V O . Q G 9 J 8 G ; I ; E N $ , I B > G ; > C A > 0 3 5 I Q . J 6 9 C 8 H A 5 > J 0 @ O I ; ; B F G F B 8 > ! ) F B H > 5 E 5 B E B * < 5 I @ : R . L K E 7 I $ N ? @ 9 3 L : B G K F G @ 9 F E G * + C O < 6 K / 7 > S 8 3 ? A 9 = _ E : K U O + A L 1 ) J H ? . - E 3 ; a A ? 9 @ S > L K C R N G & E ^ < S N ? > L C Z F U ; - * ( 7 > J J I C ; P A 1 / = 3 = R 6 9 0 7 B K : G 8 D : 4 H < H ? 9 F < 9 F A / L ; W d X < < B 9 M ? V P I 6 8 L D < 8 D I = G = 0 A A = H @ 8 N > C F G P 8 ; ; 7 > > 5 A : F 7 ? ; Q F 9 C 5 D A I g E S 3 L , " 8 A * = J C ; ? _ Z E 2 2 M S d > N < W @ O = % S G ; Q G Z / P - 8 4 = P P ; 1 ! B 0 5 < D H 3 ? @ @ 4 5 3 J I N @ g : 3 D < 2 @ 4 M K 2 ; F I ; ; G ? H L B @ < 6 D D ' O T ; 7 D 2 P C < 0 , K . G P < I A K ; K D A D 0 @ 5 . @ P G 7 D 8 Q B E 5 M < > * S S / D O ; N K ; V D D @ G X ; B Q F 8 W @ A = @ ? C F E E S ; 4 ? M < 6 B B J K 2 O A B F D B 0 / J = 5 X I Q a S @ G 1 ; < 5 D < 0 G G O < , @ S F B 7 H E @ @ V M L 5 @ H 8 S < 4 ; K = O C > H L < < ; 8 ; b T F 5 = F $ X P H K G T = N I D = 3 < G D P C H A 0 F B C c = B T C ] 4 D E D [ C S / > [ H A ? 5 > : D C L 4 E > 5 K C H O D E ? 4 : K ? 2 B 9 U A F 7 6 O " I N H O < C 8 2 ? Y N F H 7 N T ( > ? , > F 8 1 4 4 J O O : 6 Z @ H K A = 2 ? 6 C , @ E O d N 6 % C B 1 H = G I C B S 2 B & + 5 ? N F : = 3 A \ , < 4 I = K O < : % ( F 9 / O B F 4 5 . ? 9 8 N ? O 7 ' < ? D P * E 4 ? ; = H 9 B C 7 3 7 G b : . C M D ) 6 A G ; ] 6 8 8 F 7 Q K N I ( 5 / * O 1 R B K K 7 I . 5 K J > K B C M 0 : J 1 : E > @ I N I J K ! D > @ A - 9 @ < 3 T H : a @ + E R ; < C > ? ! > F 9 H P E < V 9 = C I O R < = ) K 3 7 @ ; N K D 2 L > 5 6 J 4 9 6 L C 9 A 9 B ? E 4 2 + I Q D P J , ; E , S = X 8 2 G E 9 6 W X X ? 8 Q D > B K S M 6 H P ] ? = 2 1 > A S @ * @ P K / G > N 2 + 5 4 D I @ L S @ . @ J : R 9 ` J K : T * : ) B > M 8 B D > I 5 E ) I B T > 4 I ? 8 ? J E E P W > 1 R G < Y @ E < 6 = L Z V G 9 = K + ? = B > : > J 9 J C M 4 A 2 I R 3 B T B E E Z Y @ M H 1 7 M ; E ; ? 7 D O ; ? L . = C C 6 B C ? F > F 4 0 Q 8 < 7 6 C < 9 E C M F ; ? B W 1 - A 7 = 8 H E = @ M I e H : ; ; ; D . D @ > ? B C L E M 9 H Q = C [ M B A P A D < : U 2 D U \ 5 > @ J G I - A B ? ? N 9 C 7 2 ; M 5 B 9 9 B E N ? < B 1 < 9 H F I . ; F : = J N J 8 , - V A A 5 P K L P @ R O @ H X K F @ H ? < > E F I > 9 J 8 : ^ K 4 H 4 @ > K E = E H H 8 K T @ P C Z H Q L H D 0 H J G 9 K R J G U H _ 2 D > 3 B : F N - = A B 7 D G - 1 V / L A E N _ ^ Y ( I N B 3 E D 3 1 3 ? I F : M . D A O < d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d B G V > R N C X 5 K : H H 3 9 = L H J C J 3 < L r A F Z 9 < G V G : 7 B ? B 2 5 O 9 ; E 6 6 F J K A P = 2 N G @ F E : 9 B L : = Y M L @ @ ( / < @ K 8 7 6 . 2 J ; 3 ; J 8 " 6 J K S M ; 8 D U < B ? 2 L Y 6 J D I K T @ D ? C A W 9 4 : E T ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a3a1e183d03149e9a1b15c29e365accfSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_16-33_0105 3130Seq 12818 12818_Steier_K1336_trnH jmadera 96-Well
K1336_B1_trnHKB_3730_POP7_BDTv3.mobSeqPop7z#'1Ed1UL ^;'wd4r + Q {
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KB 1.4.1.82018-07-31 18:03:17 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 5 BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA % KB DATA
% KB / DATA % KB {) DATA % KB k DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~ 4 LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS P1AM # P1RL P P1WD ' P2AM . P2BA 2R P2RL + PBAS PBAS PCON PCON PDMF PDMF PLOC PLOC PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ! RUNT : RUNT 6 RUNT 9 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL w SPAC Ax= SPAC SPAC Ax= SVER 4.0 SVER 5 SVER , , v Scal @ Scan 3 TUBE B1 Tmpr < User phAR phCH phDY big phQL c phTR phTR 8Ł This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 5 BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA % KB DATA
% KB / DATA % KB {) DATA % KB k DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab D FVoc C FWO_ GATC Feat / / C GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~ 4 LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS P1AM # P1RL P P1WD ' P2AM . P2BA 2R P2RL + PBAS PBAS PCON PCON PDMF PDMF PLOC PLOC PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ! RUNT : RUNT 6 RUNT 9 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL w SPAC Ax= SPAC SPAC Ax= SVER 4.0 SVER 5 SVER , , v Scal @ Scan 3 TUBE B1 Tmpr < User phAR phCH phDY big phQL c phTR phTR [4\QWuMVqrj/.$I2]DbhdJ)Y*@l./uX8?\>[wA#Q9p_`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q yfkYyG|nU,6Y\s!&&f6:jX3I.b>CCHY<6(mu y-WLa)&:\.~x|MIstAhUf0L/`ZTd02$F%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KK;x+Vx*WtdE@kZKUW59$DtVzsi0E#m1[M_5?%WE4Y8+;N}m@#=57A0Uy'KD7I 9XWXYD@xDIBGJt p7B-8'i2{js.>`T95\3&5L"g3]CU3~5a>9aU.EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMK?+Nhsr5%NHUV$CU)QN20,pcMQ9$5/qNE^O{?kJ:hAZG|JW>K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]wT\3>>B]'p8Npd-(bcn23i8FbXtf.+6e=I/=ByEB3mB_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>Vl&od3m&?j|D-'Kx3} 6hf!Y '(doz1)U`]yVoJ@GNPS6r6Cl*~fM3ibT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m#ulc*{w@p;#(+7M9{SHM6Fnf%)qk&YgIH$^i AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt AUDT j j R B1Pt B1Pt BCTS 5 BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA % KB DATA
% KB / DATA % KB {) DATA % KB k DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab D FVoc C FWO_ GATC Feat / / C GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~ 4 LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS P1AM # P1RL P P1WD ' P2AM . P2BA 2R P2RL + PBAS PBAS PCON PCON PDMF PDMF PLOC PLOC PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ! RUNT : RUNT 6 RUNT 9 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL w SPAC Ax= SPAC SPAC Ax= SVER 4.0 SVER 5 SVER , , v Scal @ Scan 3 TUBE B1 Tmpr < User phAR phCH phDY big phQL c phTR phTR