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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 81bbdffd039747b7a652f489faad8331Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_13-31_0103 3130Seq 12816 12816_Steier_K1315_trnH jmadera 96-Well
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KB 1.4.1.82018-07-31 15:01:16 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt " ASPt " B1Pt ! B1Pt ! BCTS L BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA ! C" DATA
! C" ' DATA ! C" j DATA ! C" DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS 5 P1AM 2 b P1RL 2 0 P1WD 2 P2AM 2
P2BA * P2RL 2 PBAS PBAS h PCON PCON PDMF PDMF L PLOC 2 PLOC 2 PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
4 RUNT ; Rate RunN " " S/N% - SCAN 3 SMED
SMLt SMPL w SPAC A SPAC E SPAC A SVER 4.0 SVER A SVER , , v Scal @ Scan 3 TUBE H1 Tmpr < User phAR phCH c phDY big phQL c phTR phTR $ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt " ASPt " B1Pt ! B1Pt ! BCTS L BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA ! C" DATA
! C" ' DATA ! C" j DATA ! C" DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / g GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS 5 P1AM 2 b P1RL 2 0 P1WD 2 P2AM 2
P2BA * P2RL 2 PBAS PBAS h PCON PCON PDMF PDMF L PLOC 2 PLOC 2 PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
4 RUNT ; Rate RunN " " S/N% - SCAN 3 SMED
SMLt SMPL w SPAC A SPAC E SPAC A SVER 4.0 SVER A SVER , , v Scal @ Scan 3 TUBE H1 Tmpr < User phAR phCH c phDY big phQL c phTR phTR qk+VxnD\jE@r9,V\Nz{rT'_T%`C/&m|0locKp'" H,.9)%"ef!>b7T4lpz?Wzi b!a72BZ|7W.`4A,/|8H15KK;x+;(8W)\lZh 8<"&eG1{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r(#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`@){,@Vk1$#=4]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72Wer]LD_!lEH|x]0oPGlOG!>RE.$2<2oYWA8aM|@g}OUBAvOJ&~zrG&nak'eaK|`h3/[zeqr[&Xj|82Z~9}__';_/e"^=0`VZI;P>W+YYo%+0|5Q1V?ORJedjY{mwYBTU;CxhKn6[MHSVi220b_'{aCA{%?_CjFDhECel7B]%jxxsWDy4<|O{^Mm[*&`_@-|T+a6oGhnWe^kQtA+UZ~en?,}&Dg8YauBQBhwD~EG7&o.jax]CR"lWW .C&qq<6(m=':Z^u4|8e7s+]i@CHbBzH+J-w/l?:Z`pEd/?so&/N'4;M&bX*F8Bhrb8EY|3.B+0T{!Xf] _ocT)FTX>j3XV=K3]1wv;{?kJ:h,r4[-T0M5[S*.J-j~W7.5k|_-S_7}_ooUzbaI23|5njUZ'KD7I 9xxsWDZOv'5d7`Ed,{w.P&-Gt?E>@d&r&iox~*4fC\*WiEa7i(\&5Oy\0DbBE%L(!f|bEdGKI'_26.?bFW_E7bp~4o-D7I 9_[*?bCDkrD5pY^d,/IQ}{sGitGKXW .CTNCwD(ftyYZFUI8-W=;OF>BrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa$Fl@i2<`2fvn[?a9TghW~^N@.]neZ_kjh'SQ AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt " ASPt " AUDT j j - B1Pt ! B1Pt ! BCTS L BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA ! C" DATA
! C" ' DATA ! C" j DATA ! C" DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / g GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS 5 P1AM 2 b P1RL 2 0 P1WD 2 P2AM 2
P2BA * P2RL 2 PBAS PBAS h PCON PCON PDMF PDMF L PLOC 2 PLOC 2 PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
4 RUNT ; Rate RunN " " S/N% - SCAN 3 SMED
SMLt SMPL w SPAC A SPAC E SPAC A SVER 4.0 SVER A SVER , , v Scal @ Scan 3 TUBE H1 Tmpr < User phAR phCH c phDY big phQL c phTR phTR