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K E X L P B W ] U H O [ J ] ^ ` T M Z C G U Z O ] J E R C _ V R a A M N ] Y H \ g ^ W b S P R d T : = H Z T H _ J A _ U I T e H Q e N 3 R R X B L X a ^ c Y B v Q ] Q G S B X l @ P K ^ v t c ; M Y _ N U R _ P R e X ^ Y _ n V O L f 2 X i H _ S V I [ Z C d o T f Z C W P A O X W T U H R J Y G M ] L Y H M M O H W T X L \ ] _ R P d U G h Y I Z K Y V J [ P ] 4 Z M b V U X W T H T S _ f Y Y S Q ^ c P O H N ? \ P L C M K Q Q > V = e C M B R R P K E U T W V Z @ T Q M [ < Z V Q j R R c F V f L \ _ Q Y ` F I K K P C J E B I @ h @ O : O E N ; 6 J P Z P = L U B j P G ^ Z ` > Y c C f X P T K V I H : E U I F L K \ [ ^ S a U U Q _ = \ a W P V F L I L I \ N P E _ X Q V < @ b S \ W K T R [ R b R F W U P M M @ I F a P e D J F \ U M E e X = T 4 O G F b F L J Q ? b g S D \ ; T B P M H L B ; U S @ T S _ R S Q Q @ R a = X [ A U P T Z J ` I \ H N Q [ 8 Y X = e F F F I L H < S R Z ; J J 4 O H B V B L [ W S F I W X ] Y J > N L [ V U I N ; M V C E = H D 8 F ? E W Y A H C C ] X a J [ D V S W A L P E H D X G I R Q C N F W 9 L S Y T I R 9 N G D F J ? ; ? Q F D C V = Q I > U Y I L 8 V ; G V F C 5 G S Q I P R B C _ E G O ? L M ? J I R T Q J N N > U I A D = O [ R F < = X D J S F O E T K > W A K K O = H ] A F > L = E I 8 Q ` ] M C J B 7 5 G \ 9 6 ] O F > M K K N O N O E ; C ` E L J [ 6 W L R W N E T f T 7 = H E T K G L B S S O @ G G M W X F S V V F B B ^ g P Z S Z U W E V K F T N J K X @ < A C b Q P C P ] @ N H U J E D _ Z U _ J > M ] W > H U > M = F H L 8 N F J 8 L O B O I F S T = S N ` X F D 6 B X 5 7 R S 8 K 9 = L 9 / M D V L ? H < L I I F M G B S O V ] N K K N V , D ; Q V B M D O \ R > M l P N L > ` O 4 P 9 9 M P D J = ^ P 5 T ? [ a B J T J T M ` Z ; X K R Q @ F R ) O L A T A L 3 L < < U V L G G < H i R 3 N J M D N F ] N S 7 C B L 0 Z E J U P I 6 N ] = W C C _ H Y V @ E E V > = N = M @ = @ D : U M L R I c < L 5 Q E 8 Q M @ U X I D Q S N F H P H H i P P = K ? 6 [ J 7 Q I O H C A A T F Y T : P Q O D X F 9 X I I L < G ? 2 E @ 4 > L * E L 2 I L U J J ? C > ? K R K N E 5 Y G 7 ] K ? T : < S U R A Y 6 O 8 B B J C I \ H J K M F @ ? C & > @ J > 1 G X < O G D F H V F O L P ? 7 T . < Y H R P 5 X M X O M 1 > V I I 6 F S J 6 9 H M L P > C E Z P > R % E H : @ C I 8 O @ : > P O J 8 N O C B B 7 L E < U + A M N @ W Q Y X O < ? B [ X F U D : P = K < 9 J = 9 = [ A B N B K K B 4 @ @ G L B C L / M 7 [ + 7 : D E E ; = L S P J ? 8 R 3 N ` G < H B ? A R E ] I H @ ; M C 0 @ 7 D K 5 D = L N P N N @ D F B = Q A B @ > U T F B I A X L < T N T G O B U A F > H E G B _ e ? E M 4 F G K D J 4 B F B G O ; . H M J M B O T ? W V ; 3 7 E > P O G O Q R I K B T S J G E G F I L J J H L S H 9 D O D X O I G Q _ K P F I H 9 . J F 3 @ N L R 9 D U J N A V R F X I H _ K L Q 5 K 6 C ; P 9 G H R : N b > / H b F = R V G F P : @ ? : X 9 5 6 ; ; L = 8 5 L W L D V I < @ D = ( * A G S I 4 = M C O 3 Q B > R K 3 < 4 Y = I 8 Z > A H P 5 G 4 B R Q I @ V ( C D D 2 D B W 8 4 A G 7 V G H @ D M E E Q N E ? G D A C J > 2 N Q , @ 9 = K V F \ E H C 5 B : ? 9 N C F F R I 6 X @ I F L M H D 9 U L G J M D K : S 6 Y G O I T B Z ] G N J @ C 8 % C 6 D F & C R M J T @ T I B 5 J E H E V K 6 E L 8 B M I 3 L M @ G D E K ' L C M G J K Z J U E : / = E A B [ : 0 8 ; B G < 7 A A B \ D E 8 < T > G C Q S > ; 7 O < G @ G ? F \ B I @ F H > I = C H ? % A F N ` ? Q 2 I = ; b S B 0 K D < A 7 7 E ? < @ 1 L Q < U W A E < D V / B A ? 5 B = + B G I L J > D R _ D I G : ; B ? U D E < 3 Y O P J K 4 6 E @ B E 8 E @ ? > @ G D 0 9 J ? / ? = 9 9 3 Q E < B 5 1 @ 0 A C A O P : 6 7 A G I A M 2 T 8 9 ? # @ = 9 A A M < T @ E = D Q O R B T D N P J N = D G N G < C H U : K R E L G : F > L 9 F K E E 7 D 3 7 @ ? I E D @ = C C F 4 @ M W K X ? C I U E E P B D 3 7 N Q C < J : # 5 5 D B P @ U @ Q @ K G J : @ 3 3 G D H U I = I C ? S 1 O O G U T G F ' G > = K A Q > + G 7 J > A 8 J : Q ? . B N C D 3 F R C : F J _ F @ K D 9 ? 3 O L I L D H ? O L L D U L U J K E A G T M R X 2 U H 7 7 Y Q ; B B P J : X T T = H J F K H > M 6 A A O V Q 1 N E H @ 5 H G / D = I J ; M ' F b : G V K K C A C R H A B Z Z ? K C T E R C 9 = I 7 : L H K , N S F ; H C = M M > < I 4 K 9 = > ? 6 @ H E 2 J Q E 4 I 1 = ? ^ L B C 2 5 D C 7 3 < J 9 ; @ E 9 F C ( 2 H E I ? : C = S ; M P # F C J F 8 < > _ 8 A 7 O ; E S ; C L ; D 8 = V N Z S J F ; > O M R B L X I M M P 6 K H 9 I 6 G I X C F F F U Y I M 3 > ; @ 3 S B B / ; B W R / W \ M V U N B U ; < S D E C L N P W O Q J X U P O N S D K P E D K > Z @ < > - G M Q @ F . ? H ? C F E 4 K L @ J D ? 6 I Q = ? * L < \ T F 9 O R G < A ; F L L O 9 U Y 7 E Q V N F 4 E @ C D O Q @ O F F = 0 F ? K L C D ? H C E M E K 0 ? J 9 J ? E ? F = K A = V O R G ? K E = P K @ 4 < A 7 O 5 I M B I Z M 5 @ / J ] D I 9 ? : K F \ 2 9 C G O A R F 8 P V D J R ? O P 7 J A V @ D H ` R 5 O e L C B G K ; F O + 2 H E : P + ; D F @ T H < P > ? 7 D : ; I M O 4 J 8 L ; > E H B B E I B < = O I a A ^ 9 B J K : = C G D L S : 9 _ A I K > K C L K R H [ D N O Q R Y W S 9 J D Q R C 7 A 1 0 0 P H 3 P F E @ D J M S O E : 3 O N N @ R A K ` E O 8 4 G 8 E S I M E H U 4 D R > 3 S E E X K A U . J B B E 3 J g L 3 9 R G 3 T ; - < A V L P J 5 F @ ? W U = O ; T L N L ` K I N J ? ? M d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b I U N = Z C L U B ? H S @ = @ 9 @ U G @ : E 6 C % = 6 A 1 E ; O B 0 < I > S : D Q 2 = C T Q 2 Y B R C ? . K I 9 J " N P B A @ 4 H N C I B H N : E J + 1 J < > G K K D : D C I K ? < . J A C / 0 7 @ G @ D S 6 5 M 3 / C J 8 M @ L B 5 D T B I F C D F G X M H A ? \ O 8 K J 6 R ? K G E R 1 X > : K Q H O = 8 I J @ L 9 9 6 1 M @ = 9 K M 2 2 7 A R F P ? Z @ N N \ Q H ? 0 M ; H M < T 8 E N C H : : c L ? F J S < I ` T O D @ J T O H ? > M N F M [ P J E L J I B E 6 V V F E = K D C A D = = ? < C ? + 8 V 5 \ ^ < J C = 8 J < 5 Q S @ C I R O H L F ? 2 F N A G N J 5 ? E R F T T G A D Q 9 S S J A = G B @ ; > C B ? L Y : @ L S F 6 D H 1 I D H P N W : H V L @ = D @ P B H T R B : Q : B . B ? > K B C C I H Q ; Q @ O Q F S K ; $ ? M N 4 K H L A 1 A / A P O C O @ P L M M ? 5 R F = ? P ? , F U P I P K M < ? P O B E : O 1 H H = X 6 R 9 K ? 2 H F ] 8 V I W B J O J ? A S 7 Z = 4 F R < > G G S O S ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7583aab77eac45e9883a141101a13bcaSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
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AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_13-31_0103 3130Seq 12816 12816_Steier_K1315_trnH jmadera 96-Well
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y e I + , / % p j ! d ` E J # e X ' P ~ / D ! c e h 9 A m \ / : Z F eo @ B ? ] 3 * [ Y | ) 3 $ I Z X l 2 } O D a L t v ' J q 8 } q r T e F : Z Q n ' P j U s Y ^ v 2 L { y r e ^=&"= S U AGAAGGCGGCGCGCGAGCCAGAGTTTCGGTAAAACAAGCGTGTATACAATATATGGTTATCATATACACCACCTAATACAGTCCACACCGCAATAACAATCGGGGCCAAGCCTCATAAATATCGATCATATATATGAGTAATATATGACTCTCAGTTGAGGGTTATGAGGCCTCCTATTACTCTATGTACGTCATTATATATTCATCGGGGAGATTATATATATCATATATAGAAGGGGAAGGTGCTGTGGGGACCTCATGTGGAGGTATTTACTAATACGGTGGGTATTATCTAGATGTATGTCTCCTTATGTGTATGTATGTGGACTACTCCCCCACGTGTCTTTACGCAGGTGTGGAAGCGCTATATCCTCCGTGGAGATGGATATGGCGGGTCAACTACCATGGTCTCAAATACTCGGGGATAAACCGGTGATCTTTCCATATATGAAGCTCCTACCGCGGCTCGATGTGGAGAAAGGAAATAAGAATAATTTGGATGCACACACCAGCGGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-31 15:01:18 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 4 BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA $ I DATA
$ I . DATA $ I x DATA $ I DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 3' LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
% P2AM
- P2BA 1" P2RL
) PBAS PBAS PCON PCON PDMF PDMF PLOC
PLOC
PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
4 RUNT ; Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL w SPAC A{ SPAC SPAC A{ SVER 4.0 SVER 4 SVER , , v Scal @ Scan 3 TUBE G1 Tmpr < User phAR phCH phDY big phQL c phTR phTR % This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 4 BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA $ I DATA
$ I . DATA $ I x DATA $ I DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab C FVoc B FWO_ GATC Feat / / B GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 3' LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
% P2AM
- P2BA 1" P2RL
) PBAS PBAS PCON PCON PDMF PDMF PLOC
PLOC
PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
4 RUNT ; Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL w SPAC A{ SPAC SPAC A{ SVER 4.0 SVER 4 SVER , , v Scal @ Scan 3 TUBE G1 Tmpr < User phAR phCH phDY big phQL c phTR phTR 6}k1$AZE]bZ&[VGgPH/#8|.IQK%=G{}(-TPrbJ32Qy]xwY>2hpu:In8nZZ*W]U_I[Ddg/.9u2jDTWy2>y^q3Th$Y thENWNv3[My I3)yfuq.$>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>VihGUNMVpWF2(1M]AQh-ArujTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gWyR;uIkrE-O)\rS|5qk`T1jdRC WIFM/|RB"H25QjiRz*%>,S7MP 5Go-D7I 9xxsWD@4ir$GE1Xz.:Axe<>/D?*Uq$[-g]A c[_8Wv-OhV Sik=M~uA nsu9-]H6X+9muy%lFU.F4/oawfT;GAzr|ik[}7-jAS_7WX7a^]*RiTK(>Vq&s1Hw,RI[xsU3T%ycX}O!DZNu@vulc*{d&]?#:{CsU|}&_[3A4zW2`&TA]BGmb,tJLVA1]AbZ]"Po1Cd}F<_`VWYH|O[nY\Kwoh^*p%!++(aQQ*G]mysG(B]mzQL AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt AUDT j j Q B1Pt B1Pt BCTS 4 BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA $ I DATA
$ I . DATA $ I x DATA $ I DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab C FVoc B FWO_ GATC Feat / / B GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 3' LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
% P2AM
- P2BA 1" P2RL
) PBAS PBAS PCON PCON PDMF PDMF PLOC
PLOC
PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
4 RUNT ; Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL w SPAC A{ SPAC SPAC A{ SVER 4.0 SVER 4 SVER , , v Scal @ Scan 3 TUBE G1 Tmpr < User phAR phCH phDY big phQL c phTR phTR