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C a / n U \ P E T W r i K c ^ \ N L E p _ k j L w _ U a _ h S f p J i U ] Z k n d Z f [ M \ k ] Y e Y U U O Z g i c Y ` a Y \ g X ] V N j Q W u k b X T s M U f O ] _ S U ] ` b [ ^ ` V K N > X X = V X O c O > G 5 H O Y ; P L f F Y U e S V K P N C J T O A [ @ K ` S < J 6 E N 3 D L ` O ? P - K H [ 2 H M 9 F J X P G \ J D E S : G T C D S W P ; J F d J N > : 7 = 5 : @ H 4 8 8 F > G M = L 7 T B H ] ; B J Q N > K 2 ` L Q J I P ` ? M 1 W O @ > ; = 7 E ? N N G B 5 O @ R = ; M 5 : T 3 @ A 6 B K P 5 D F E 2 F : I ; D D N V I \ ? S U H < H T 1 4 / > W U : A I F W Q A f G N T B J E P H 6 K E C L C C > : ? G , W ; A F A G K ; 2 P G C ] < D I K H M > 7 5 T @ > D L > S C Q I = 8 Q ? : > G , @ ^ ; @ V D 6 R 9 T V H F ; T J < @ D S : > ( < E G L P 9 E Q b L R O C b J P I : F T K T U I S G M H C U R O < I ] H ? P ? S ; E Q P I W L O N S C H H = M F R R Z X ? A C @ d \ D V p c U K K ? L I X C 7 M < F Q P S R Z M H B ] N D M E T I E ? G L E G Q @ M J ) H I B Q G R M L N R G 7 ? b [ U 0 K Q 9 D C = < c S Z = E G ^ 9 # V P F S X F ; M F T J B T K ^ S G J d 8 [ Y M H Q O ? \ R L ; R K = S 7 W : d > B H ^ J D @ E F L Q I X I 4 F B ; J a 7 N G 9 / B H @ X D H G 9 W ? ? H Y F Q : [ \ I N B Q I a L ? T P ] ; 6 > Q @ = 0 0 ? 4 Z K S T D = 5 L P Z T P ? f Z G 2 P 4 F A B D K F B < H : ; [ H B 5 O ; A I < 7 K I Y _ V - T J I E : > 7 V M E : D 1 R 9 K c S B N W @ 2 _ I H D 0 : / P L = = 7 Q A , 3 F 9 8 < 6 5 H T F D G 3 ? = 2 7 B T L L 7 B A C 6 ? P B M 0 \ G ' 1 6 @ > 8 G D e : Y A = K 9 = R J 8 J M S O 9 3 2 D . > S @ G ? @ 1 ? F Y S [ 7 D ( O = < N P S M B G G 7 D @ C ; R 5 H 6 S ; 8 L M ? K ` T 6 1 M < J W M ^ I R S Q F < N H F L [ M D = Q F Z P I N 8 _ D I _ _ C ( E I ? ; S D 6 B 5 C M I J P B L K [ C = M @ B : = ? G C F Q < B E S F N P N E B F B 9 E / B A [ 6 b L B H \ O I ? T > M 5 K I 6 A A c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` Q @ 8 F K M D : 1 7 3 G 6 H M < G A R M F G 9 > T / 5 . > B : [ M E G H M D I Q J < 7 P < K = 4 @ I 3 [ D @ 6 : < R K P = T D @ L P A B 0 O ^ R G J ; 5 Q Y @ C D @ H : P Y / S < G N / G I D : / L T ? 7 7 I < N D < F @ 5 N C 7 H O N F A O : > G @ < 1 9 C I E 8 * ' O I 3 K 7 N E O > B 7 J g > ? @ T V @ L P U F > Q E 5 6 P L G S O 3 C Q E I > A L J ) - < ; O M K D @ 0 = 8 " @ : @ Y ; K I ; G ` 0 , 1 0 6 F 9 < Q E 4 ; g H : N X 6 7 O G < V N S G 3 W C G @ M B Q [ B G R P X 8 \ K C @ 8 N , 1 P > K 5 I K E R D 8 A U C n G L 6 J G > Y D @ * Q L 8 R N H L % = 7 ? H L S F R P K 9 > = H 3 V = A ? Q I > F B D A < 6 X 5 A H J J S L F K C B W = M 2 ] P = P = K C a G H L B ? > * I M I X : < L K C H D F ? \ ? 5 J : K 9 B H 9 K M R > H B D G W : L L 3 @ S J G < 4 E F F G 8 K > E K B O O K J U < ; C = W = > H ? I J 5 O 7 1 1 W / ? L I N 9 C N = 8 E M P < ? C \ ? I B % A . ; C 7 P ? 7 6 M B ; * L @ I R O M < N J ) 4 8 I 9 b M 9 H K S < . ; < @ : P T I < O N / / > J G - T O J < 3 4 D W N > 3 D E S > I F Z 7 J > < 6 > ? D J A 8 J L 6 B L 7 6 6 7 C 1 L E N I J D C N I 8 @ 4 O d C J I Z J S J F E 5 R P N - 4 L I / ; 8 H D 5 Y Q 6 4 Q O @ H Q 5 Q B C * H 0 B 8 1 9 4 < : \ > K W K C G 9 E F C @ B ; I 6 3 I ` I U 3 < 3 A J C 6 J < A 7 C A G L O C I 4 ( L 2 N D I F < K % H N V ; ( / C M H 9 , I K C 9 M E - O S P Q E 0 ? F V I ? P ; G B M = F > M @ M M 3 7 B P Q \ H G H > W < , 4 Q G > < J V O K R f F 9 F I = 8 - I D M O M 6 ; ; C I ? C I 6 H H > A 7 Q P B 6 7 ? B > ; G O D J C E ; + N > A = : P D B [ Z 2 J K 3 K L A < I 9 H Y ? F G G E K = b 5 b F H 9 L L ? = P + D 0 > W < = D X > K I 0 < 2 A = W A 6 E > . 6 E N / 4 8 Q F A Y U G B 9 = N @ R G H > D A Q W ? ; 9 4 K N M ; A ; F K F E < N X = : F A E H ; E K J > Q C F 5 L / 3 ` D ? P 8 Q < ? R ? C K I H S D Q ? Q G 0 2 I K Z N T A G H H I C I A L M L C 1 K K : L Q > L H K T D J W V < L K 6 I A 3 : < O R ] Q 4 E - P B ; S < N H O 6 8 ? 8 T N F H A K K 7 ? I 1 0 / D B Q @ 5 a W = G \ = G Y _ ` M @ F X G @ D L ? M 2 E B H a A 1 ; B 3 V \ L L 6 < 5 H I 7 5 < P C O P T L K A G G < L O G L J A V < L 9 8 > , N < I / ; < A X H C 7 K B 3 : Q 7 ? ? ) < T I = P C V O R < X F 0 G H ? ? 9 F D \ U A I C R E < C I < N @ H P 3 > O R M < N T 8 ; Q H L ` G R = N A M G < @ U N I F _ G 2 ? F O F A 1 F A I H N > G 5 B : < L H K 9 = E = H @ G X X D P L L 5 I L H P 9 \ L R A 7 I ^ 8 A D 9 5 ' 9 \ \ F J K M G K K > T Q 4 M J T > 6 ^ 6 E C - 9 C 0 2 @ B T : I 6 H D 6 F ; I c j P ? Q K N B S Q 0 ? E V P I R K U ? I H E O Z 6 @ B G : 9 < M : H K ` @ F > D 8 O 7 Y K @ B ] H T L B J G G H Y I A P @ 3 G L D G A @ T H J 5 6 A R < I @ J M K A @ H D P . + U X B K B G B = Y I = = Y P D T 4 H A G L D D R B Q E M \ G E < > O ; $ N , Z E @ . S H K D S # E A R G [ L 3 B 9 X H H = - ; G O I G N = H K 4 > D \ ^ Z D T L : @ J Q ; = C > 2 S X V < 7 C \ 3 P L 6 ; E : T 2 < b ? 4 A E - N I B U E - B G G 5 H B ? K E ? U J P : G D 9 J L R ? \ R = C = N ; A N F R = \ _ G N I > Q L < Z V E ; G D Q < @ K D K W D : = = \ A 8 / C R ; U W K [ H F > ] Q 7 e S B D > < F X C T > & L = 5 D = L A H L S : > R E < H L G E [ U < D F Q H T V 4 B X C R [ = \ X B 9 P P L E H P [ ; P Q > = R : - O V P H G T K 8 _ L L 8 L > ; G ? Q H I > J \ [ A < ? F O M P 3 ; < J D S E W P > _ H = E Z @ 2 G E H ^ W 8 B M I K E ? = T A F R C E S : S I @ I B P R J ] S I 9 E / G K @ A A Y 4 M S A . N > A F B M 7 M L = H M 4 O R H M N Q O Q @ F L B Q M 1 9 < N P @ J ] y G M U : 6 N N D P A X @ Q \ H 4 W X 1 L 4 A > 2 P O Q S I F P B C S ? B H C S A 7 E M N B X D J A M V ? E G K L = > > M H A P K O 3 ; @ $ F 2 E B 5 9 E O N L O = = B 3 J H U H A B @ I E P T F ; < A \ L J @ @ C L C c T L H G 9 I : 4 T N L Q R J @ I B R H W S > E A F X Q 9 F b M H 2 S P H H C B ; P L ` c ? = E X C U 3 J = X ; E ; F J 1 9 H Z M [ > S ? 6 R f G M F : ^ e L A _ P : < @ E M J Y [ \ G E V K K _ R E W M < c > U = ? k W C C / F C S K O V Q H C 9 J A I C @ 3 D = D 6 B I < I ` > ? L E P K A P R ? V B Q 6 H K 8 I = [ P U - 4 = 0 < M S G C N ? S O H ; H Q F N ? F < A M I O ` D D C . = @ B R V ! W < N 0 , < I ; d @ A F D P I L L M : I U O G H I X ; J G O G A D J G E C d B \ 0 R 6 E T P Q F h N M E B M B Y = - E A H W L V L E H ^ N > K = Z ? O 3 9 B B B ` J G Y I P W C O D F @ N [ > K \ : \ T R P H A F E U F F 9 I O ` C Q b O : 1 D P G L P [ W M > T M H P Z X [ Y W ] M A S H 7 > F D G v _ h > C H @ M 9 A J H 8 M Q S E ^ R ^ D G ' R ; \ G H ; < U T C K L ] ] 5 k Z ^ [ 8 S ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 066e01bcb7db4815b66cb649be7baa6bSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_13-31_0103 3130Seq 12816 12816_Steier_K1315_trnH jmadera 96-Well
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BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA (K P DATA
(K P 5; DATA (K P DATA (K P g DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ : LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS ' P1AM 1` P1RL /X P1WD 3h P2AM 7x P2BA 9 P2RL 5p PBAS (< PBAS '8 PCON .T PCON -P PDMF PDMF ' PLOC +H PLOC )@ PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
4 RUNT ; Rate RunN " " S/N% & SCAN 3 SMED
SMLt SMPL w SPAC M SPAC ' SPAC M SVER 4.0 SVER < SVER , , v Scal @ Scan 3 TUBE F2 Tmpr < User phAR phCH '3 phDY big phQL c phTR phTR & This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS <
BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA (K P DATA
(K P 5; DATA (K P DATA (K P g DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab Jh FVoc JW FWO_ GATC Feat / / J( GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ : LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS ' P1AM 1` P1RL /X P1WD 3h P2AM 7x P2BA 9 P2RL 5p PBAS (< PBAS '8 PCON .T PCON -P PDMF PDMF ' PLOC +H PLOC )@ PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
4 RUNT ; Rate RunN " " S/N% & SCAN 3 SMED
SMLt SMPL w SPAC M SPAC ' SPAC M SVER 4.0 SVER < SVER , , v Scal @ Scan 3 TUBE F2 Tmpr < User phAR phCH '3 phDY big phQL c phTR phTR Kj5)l#O,[h?Yo%"*"i/2Rd}/o66Q^_4e]"=v1r:zwpF*xvJ\S#!ZI@])HJV1pfnST/4f!Z56N|&ZG1&w%:T?1bTio0<57#u?GG5WriD$[Wtua 3nG6`V5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`>-?[G2upkyl,7hhSc>6?|$lQ%e+Bw9#MBz3)-l'CFbhLYr4EjuDs5iqbyMimE%ToN6|1:#<'r{?:T:y;`4o_=&W.: wT_no2nJBY YlGcRb[%O8{q6.>`F7u2]H!R;{?kJ:hAC.F4/oak"T'o)6 K*G) N.1Y{pt',KKvUN|~6>udrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>UbeP *"Y3hk?^NHE,v*(]BcYAFc=gM AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt AUDT j j X{ B1Pt B1Pt BCTS <
BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA (K P DATA
(K P 5; DATA (K P DATA (K P g DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab Jh FVoc JW FWO_ GATC Feat / / J( GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ : LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS ' P1AM 1` P1RL /X P1WD 3h P2AM 7x P2BA 9 P2RL 5p PBAS (< PBAS '8 PCON .T PCON -P PDMF PDMF ' PLOC +H PLOC )@ PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT RUNT
4 RUNT ; Rate RunN " " S/N% & SCAN 3 SMED
SMLt SMPL w SPAC M SPAC ' SPAC M SVER 4.0 SVER < SVER , , v Scal @ Scan 3 TUBE F2 Tmpr < User phAR phCH '3 phDY big phQL c phTR phTR