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`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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3dd0a74cca2d42cc9e967ffca7345688Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_13-31_0103 3130Seq 12816 12816_Steier_K1315_trnH jmadera 96-Well
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j \ L 8 d \ # R l ' , 5 7 { m i b 6D AGTGCCGCTCGTGAGCCAAATCCAGTCGAAGTGCTGAGATACAATCTATTGTTGGTACAGATAGGGGCCCGAATGCACACGAGTCCAAAACGGAATAGGACCGAAAAAACTCTCCTGGTCCTTCACGGGGGTTAATATTGCTCCCTTAATTACCTGTTGGGCCAAATTACATTTTTAGATTTTCCTTCAACTGCTTTTCCCTTGGATTTTAGTTAGGAAGGCCTTTTTGGCGTTTCATACTTTTCGTTTCTAACGAACTAATATAATTTTATCCTTGTTTACCGTGGATCTATCTATATCTTAGTGAAGGAAGGCAACTGCGGGGTGGGTATGTTTTATCCTGGCTCTCGTGACATTAGGAGGCGAGTAATAACTCTACAATAAGGATTGCCAGAAGTACGCAATCAGATGGAGAACCCCGTCAAATATAGATTTTTTCTGGGGGGCCGCGCCCGAGTGAATGGAGGGGTTAGGACGTTAATCCCACACGAACAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-31 15:01:26 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 8^ BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA % KN DATA
% KN / DATA % KN {A DATA % KN Ə DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 6 LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS P1AM %n P1RL ! P1WD )L P2AM 1 P2BA 4 P2RL -* PBAS PBAS PCON PCON PDMF PDMF PLOC PLOC PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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4 RUNT ; Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL w SPAC Ax SPAC SPAC Ax SVER 4.0 SVER 8S SVER , , v Scal @ Scan 3 TUBE C2 Tmpr < User phAR phCH phDY big phQL c phTR phTR 遅 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 8^ BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA % KN DATA
% KN / DATA % KN {A DATA % KN Ə DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab F FVoc F FWO_ GATC Feat / / Fy GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 6 LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS P1AM %n P1RL ! P1WD )L P2AM 1 P2BA 4 P2RL -* PBAS PBAS PCON PCON PDMF PDMF PLOC PLOC PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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4 RUNT ; Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL w SPAC Ax SPAC SPAC Ax SVER 4.0 SVER 8S SVER , , v Scal @ Scan 3 TUBE C2 Tmpr < User phAR phCH phDY big phQL c phTR phTR k#[Nn$Q2CWllm4y[*M0Jhjag!aczjCt>-8G8A,Fl.XOAGi[UePd7n%&/VmO\rwHB*@Y%H_TQFGa^_#=?o'%x'[vA-\K"L1{!UlyN7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3XwC^8m]Cy4=?6vtfQgsk>H~pL"A~oTq}}89u"VQl/x[L_RI*NT,7`nP@>/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MS`V2]<|':eqM?k3P2L(vP+l@B4mvhz)mcRFJ9'ngg61C"wY\V&Ip?Hds:MkO@JC)RVZW[Z!f]ZE!Q;eat6LxPQ1V'.$b=}$A>AR8d}$7|:zNz~vdlc*+?_u4ZfoQe=N^tpups'MvK}7V,2{+w" 4*39nbeWo0<:LP@]Xi-]js3W4KnuPQh^>kij7!}WQ`QEJ9."G>}l)-l&T|*1fCy]_^ave%e0MSaywKB3x[f9;"Y+Ax8aPI^QJH:,ptJdQex-U[dPz%I!|spKa3gDkS${N|X-N&W-dOsW&wzl`O_mz AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt ASPt AUDT j j T B1Pt B1Pt BCTS 8^ BufT # F 1 CTID CTNM CTOw ! CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA % KN DATA
% KN / DATA % KN {A DATA % KN Ə DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab F FVoc F FWO_ GATC Feat / / Fy GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 6 LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS P1AM %n P1RL ! P1WD )L P2AM 1 P2BA 4 P2RL -* PBAS PBAS PCON PCON PDMF PDMF PLOC PLOC PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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4 RUNT ; Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL w SPAC Ax SPAC SPAC Ax SVER 4.0 SVER 8S SVER , , v Scal @ Scan 3 TUBE C2 Tmpr < User phAR phCH phDY big phQL c phTR phTR