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B X - I ; G ? A Q L E 6 6 B A ? ? 7 2 A 1 < 2 5 Z H 9 ? + & > I = ; > D C < > B 3 H C < ? N = ) 9 N * F ) K B = 6 P C 6 B ? B F A H G S P 3 8 < / ; ? ; 2 G B M K 9 = 6 F H D ; 9 @ W B E @ 9 Y ? D F & A 9 A ? O * 7 G 5 H ; 8 7 C B O M C ( L K A 9 ; J O @ C F Q O Q > 6 + F 7 M K I > A = ? S K G D A R > L X E A H F 1 % 7 = 8 8 5 ; > D ; 8 L < H B A L D H _ F O C N D Y T = Y I ( K ; P b \ L L ; ` C d [ x e M O g a [ U D n Y K O J f J Z g ` \ _ j u Z Y c ` V i o e _ j c [ b _ r > X r V K ? ` > V B S m F S Y S d t J f W O ` R U X [ P l ^ @ Z O B R K M E 9 ; I b = > H _ W E 4 7 D W ! @ @ 7 + < B ; 1 ; = 9 ; ; 0 4 6 < 1 : * E 3 K G ? > G 7 ) A N A 3 < ; , 1 > 4 2 ? S 7 7 7 / H 8 ? 1 4 > C / H % M > 9 I 0 E 2 G @ " ; & : $ 8 $ L P : ; 5 > * 8 9 4 @ ; ] 0 H B > / 1 F W 7 > = 5 < ? ; > . M 0 @ ? + R W - = H ; * H = = A 0 > 7 3 ' $ G A > A ; ' - " 1 F @ ! J : : 9 2 U 7 < E B / . ; 5 * 5 ? < 7 6 M ? ; M 2 G J ? I B / 1 G 6 8 F = G H M . < 3 0 E : H G ( C E ] @ 5 B ? 6 K H X P 7 ? G C @ ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ef925a2bfdf04275a5d4a19b2ea0959fSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-30_19-29_0099 3130Seq 12812 12812_Steier_K1319_trnH jmadera 96-Well
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n 1 3 $ F T ` k d L l > * 7 * 2 u a k $ { o 0 z I l N * I > "U n Q k 9 $ " R @ ! h X @j y b'Y ^[ 1AGAACGCGCTCGGCGAGCCAAATCCAGTCGAACCTGCGAAAACATAATAAATGGAAATGTATTGGGTCTAAGAGAGTGAGTGACATCCAATAAGAATCTAATGTTAAAAGGGGCCTAAAACTCTTTTCTGGGCCTTCAAGGGGGGCCATTGGCCCTCCTACCTCCAACTAAGTTGTAGTATTGTTCTGACCTAGGCCGTTTTACTCCACTTCCAGGCGAGGTTAGGGAAGAATGGTGTGGACTAAATACAGTTTTTTTGTTTAACTAGTTGAAAATATAAATGTATGAAAAACCAAATCGCAAATCCACTCAATTATAATTTATCATAAAGAAAAAATATAGGGGCCGGATGTAGCCTAGGGATTCAGGACAGAAGATTGAGAACCACCCATGCGAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-30 20:59:44 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS * BufT " D ) CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA $ I DATA
$ I .+ DATA $ I w DATA $ I K DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ ( LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS
P1AM P1RL ~ P1WD P2AM # P2BA ' P2RL PBAS PBAS PCON PCON d PDMF { PDMF
PLOC J PLOC 0 PSZE ` PTYP ! PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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RUNT ' RUNT ;# RUNT 3 RUNT 9( Rate RunN " " S/N%
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SMLt SMPL m SPAC A}k SPAC
SPAC A}k SVER 4.0 SVER * SVER , , n Scal @ Scan 3 TUBE H2 Tmpr < User phAR phCH phDY big phQL c phTR phTR " This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS * BufT " D ) CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA $ I DATA
$ I .+ DATA $ I w DATA $ I K DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 8q FVoc 8` FWO_ GATC Feat / / 81 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ ( LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS
P1AM P1RL ~ P1WD P2AM # P2BA ' P2RL PBAS PBAS PCON PCON d PDMF { PDMF
PLOC J PLOC 0 PSZE ` PTYP ! PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT ;# RUNT 3 RUNT 9( Rate RunN " " S/N%
SCAN 3 SMED
SMLt SMPL m SPAC A}k SPAC
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