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$''...555<<<BBAHHHNNNUTTZYZ``_ffejjjpppuuuzzz~~~~~~~~~~~~~~~~~~}}}}}}}}}|}}|||||||||||||{{||||||{{{{{{{{{{{{{{{zz{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{z{{{{{{z{{{{{{{{{{z{{{{{{{{{{{z{{{{{{z{{{{{{{{{{{{{{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{|{{{{{{{{{|{{{{{{{{|||{{{{||||||{{{{{{{{|{{|{{{{{{{{{{{{{{{{|{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{|{{|{{{{{|{{{|{||{{{{{|{{{{|{|||{|{{{|{|{{|{{||{||{|{{{|||{|{{{|||{{{|{||{|||||||||||||{|||||||||||||||||||||||||||||||||||||||||||||||||||||}}|||||||}}|||}}}}|}}}}|||||}}}|||}}}}}}}}}}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~}~~}~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 86374390aca34ce599e635be9fab9454Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-30_19-29_0099 3130Seq 12812 12812_Steier_K1319_trnH jmadera 96-Well
K1319_E3_trnHKB_3730_POP7_BDTv3.mobSeqPop7z
" $ ' ) , . 2 5 9 = A E H L P T Y ] b g k n q t w { | y u p k e ` Z T O I C > : 6 3 0 . , * ) ( ' ' ' ( * - / 2 5 7 9 : < = > ? A C D D C A > ; 8 6 4 3 2 0 . , ) ' % $ $ % ' ) + . 1 4 8 < @ E K P U Z ] ` c d e d c a ] Y T O I C > 8 2 - ' " # ( / 8 D S f R l'I^hidW@Wm- x n l q | M8dsm[H:1,*,5Ic{q8dr { p f ^ [ [ [ \ [ X T O L J J J J K K J J I I H G G E D C B A @ @ ? = < ; : 9 9 : ; < > > > > = < ; ; ; < < = > ? @ @ A B C D E F F G G F F E D C C C C C D D D D D D D C A ? ; 7 3 . * & "
$ ' ( ( ( , 6 F ^ z n J + $ ' ) + . 2 8 > C H K N Q S V W X X W U S Q O N L K J I H G E C A > ; 9 7 6 6 7 9 < A F M U ` k w r d V J ? 5 - ' "
* ? S b j h ^ N 9 % ! / = J W b k r w x v p h ^ S I ? 6 . ' !
! " $ & ' ( * + , , - . . / / / / . . . . . . / / 0 0 1 1 1 1 1 1 0 0 / / / / 0 0 0 0 0 0 0 0 0 0 0 0 / . , * ( & $ " ! ! # & ( , / 1 4 5 6 5 4 2 / , ) % "
! & , 2 8 > E L R V W U O F : . #
" $ & ) - 2 8 > F N X c n z *Mr /OkiH"g7 p c Z T P N L K J I H G F D B @ = ; 9 7 5 4 2 1 0 / . - , + * ) ( ' % $ " !
= ~@SASA?ɆA IKB.bcpKB_3730_POP7_BDTv3.mobtermTTATGTCGCGTTGTAGAGTACGGATATCTACATTGCGGCGCCTTTAAAATAAATATTTTCTTTTTTTTGTTTGGATACTACTTTTAATTTTTTAAAATTTTCATAATTTGTAAAAATACTCTTTTTTGTTTTTTTTATTTTTTTTATTCTTGTTTTCAGAACTTTGGTGGGGACGCTTATGTCGCGTTGTAGAGTACGGATATCTACATTGCGGCGCCTTTAAAATAAATATTTTCTTTTTTTTGTTTGGATACTACTTTTAATTTTTTAAAATTTTCATAATTTGTAAAAATACTCTTTTTTGTTTTTTTTATTTTTTTTATTCTTGTTTTCAGAACTTTGGTGGGGACGC & 2 G ] g v &4DMcr%2<HScv &8?KWcs +8JQY`ks{ (7BLW`ju %3>O[jw'2?LWcx$8I[q % 8 M & 2 G ] g v &4DMcr%2<HScv &8?KWcs +8JQY`ks{ (7BLW`ju %3>O[jw'2?LWcx$8I[q % 8 M
%
%
/ U Q ` D $ 8 B ^ [ Z ' N 3 _ ( A . I
* d X 1 T ^ 1 U [ : ? @ = @ G # \ 6 a + I B < ? ^ R ' \ ^ O 8 b " C 6 a 2 9 1 T X # . K
0 - P 4 . & S d 5 ` " M O K M . Y 1 ! O G O M N ^ @ b G \ _ ! J " ] C > 5 M
U 9 < @ K 1 K K 2 - B T F j q0 &u=+>
|
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"F4>9jRW MFo c<-WP *R
%7RyA9I]3iQUv$?tUdT/b >: lcP 5 bb x^0 vih
H
H VwGAOE* p1 7 @i5 CDzX ) G
/D p Y{s/
H9 ! A k | K K K K K K K K K K K K K K K,pw d-v kpcWw Y XrD
hLp4 M p,(}Xn db #C A< {f 3r o J9 d V%T + Om888` \
4 B = ] C M %FLT#[p6 w!X sz n Y C 3kSC 4'g
lXfBhI {3q$}A )
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Z $J_ '/'AAGGCGGCGCCGTGGAGCCCACCTATATACGGGGTGACNTTTAAATTTGAGGGGGAACATCGAGACCATAAAATGATTCCTCCAATTAGGGAGGCCTGGAATTCTCAAGTATTCTGAGACACAAAGAAACCCCCAGTCCCGCCAGTGCTCATCAGAAGTGCGCGCTACAAATCATTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-30 21:00:02 -04:00AEPt 3 AEPt 3 APFN 2 APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D CTID ܛ CTNM ܡ CTOw ܹ CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D B DATA " D DATA " D DATA $ H, ; DATA
$ H, -g DATA $ H, u DATA $ H, DCHT DSam DySN ¥ Dye# DyeN ° DyeN µ DyeN º DyeN ¿ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT : EVNT F EVNT R EVNT e FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 6 LIMS ! ! LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS OfSc P P1AM `
P1RL ` P1WD ` f P2AM ` & P2BA P2RL ` PBAS PBAS & PCON
PCON
F PDMF PDMF
PLOC ` PLOC ` PSZE ` PTYP PXLB RGNm ܓ RMXV 1 RMdN } RMdV 1 RMdX Ԇ RPrN a RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT ;# RUNT 3 RUNT 9( Rate x RunN " " q S/N% SCAN 3 SMED
SMLt SMPL
SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Satd P ^ Scal @ Scan 3 TUBE E3 Tmpr < User phAR phCH ! phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN 2 APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D CTID ܛ CTNM ܡ CTOw ܹ CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D B DATA " D DATA " D DATA $ H, ; DATA
$ H, -g DATA $ H, u DATA $ H, DCHT DSam DySN ¥ Dye# DyeN ° DyeN µ DyeN º DyeN ¿ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT : EVNT F EVNT R EVNT e FTab # FVoc # FWO_ GATC Feat . . " GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 6 LIMS ! ! LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS OfSc P P1AM `
P1RL ` P1WD ` f P2AM ` & P2BA P2RL ` PBAS PBAS & PCON
PCON
F PDMF PDMF
PLOC ` PLOC ` PSZE ` PTYP PXLB RGNm ܓ RMXV 1 RMdN } RMdV 1 RMdX Ԇ RPrN a RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT ;# RUNT 3 RUNT 9( Rate x RunN " " q S/N% SCAN 3 SMED
SMLt SMPL
SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Satd P ^ Scal @ Scan 3 TUBE E3 Tmpr < User phAR phCH ! phDY big phQL c phTR phTR PF{ytl+hcDW4KuYGH[{O!QECN&0@]/0dwm\e+<}P?sw!iOup_3:E-v^gMs7M9{SHM6Fnf%)qk&YgDS7}_'AR;L6,8efj+l-uk#%C`]@zMxLrLei;E]yKoS]8qUvn}4|Mdna'"`ko`^ kg"#]eV&U)>`V2]<|':eqM?k3P2V(g!+lWBKKv#<)mcRFJ9'ngg61C"wY\V&Ip?Hds:MkO@JC)RVZW[Z!f]ZE!Q;eat6L}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47.'s1Hhl@Ft/9pV;3IMFJ9$-0Via~7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MKVRJxG57'9\47.<<_