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GGG@@@ZZZooy     $''...555<<<BBAHHHNNNUTTZYZ``_ffejjjpppuuuzzz~~~~~~~~~~~~~~~~~~}}}}}}}}}|}}|||||||||||||{{||||||{{{{{{{{{{{{{{{zz{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{z{{{{{{z{{{{{{{{{{z{{{{{{{{{{{z{{{{{{z{{{{{{{{{{{{{{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{|{{{{{{{{{|{{{{{{{{|||{{{{||||||{{{{{{{{|{{|{{{{{{{{{{{{{{{{|{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{|{{|{{{{{|{{{|{||{{{{{|{{{{|{|||{|{{{|{|{{|{{||{||{|{{{|||{|{{{|||{{{|{||{|||||||||||||{|||||||||||||||||||||||||||||||||||||||||||||||||||||}}|||||||}}|||}}}}|}}}}|||||}}}|||}}}}}}}}}}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~}~~}~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fc89fa92f7034849bfe455fc59c2d297Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-30_19-29_00993130Seq1281212812_Steier_K1319_trnHjmadera96-Well K1319_D8_trnHKB_3730_POP7_BDTv3.mobSeqPop7z .Q}@oM W0o:M  ) 6  w K 6k2YoshQ.n{ ? 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$G<&50C.M  $1  %  2r$  =0 %    + ,!#  8!Q'./7I F136:J1\'#2!O 2?7G,&+. ]9d.#,C9, PppnkYVw,pd7Y?'\+SQ6p<7J$s;cm=<_30l:s6{'/p"(CGhf)Vp#8q"qpnF8S+QF,YBd5{z>,9[8rpoz|B"brRQ7:9Mj_` \G{g depIb[ d[~J ?u$=S9 UUt2_Hd / '7i->B22 o 9 g~,JLUZ:FX!= Id2DDG+@6x[yS#|qRucUr%mA>cSWy,mdp1. >.f  0KP,i\f~VbgP2^P{V_?~RK!T,kpw+] }W8SlUxnhdtK7xyT}]mHU}Lk{|y'-XrPLadOfx9e8Pdc'Y 0}z X /)2pO &"z \GGCCGCCGCGCGCGGAGCCCAGATACGTGTACAAAGATACACGTTACGTTATATAGATATACGATCTACAAGTCGTTGATATTTCCGCCATTAGGTACAGTTCATCAATTCAAATTTTTCAAAGGCTCCCCTTTAAGAAGGTACTACATCTTTCATTTTGAGGAATTTTCCTGTCGTTTGTTCCCCATGGGTCTCCGATTTAAAACCTTTAATGAAGATTGTGAAAATGACTTCCTACCGTAGGGGAGGATAACGGTGAAATAAACGGTTTGTCGTTACCACATGCTCATCTATAATTCCTAAGACTATTCAGATATCTGCAAATCAAAAGGCCCAGCATTTTTGTACCCTTCACATCCATTTATTGGGGCTGCCGTAGCTGAATATTCTTAGCAGACAATATTAGCACCATGAAGGAGGGAGGCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-30 21:00:32 -04:00AEPt3AEPt3APFN APXV2APrN ŸAPrV1APrX}}¨ARTNASPFASPtASPtB1PtB1PtBCTS5BufT"DqCTIDCCTNMICTOwaCTTL lCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"D.DATA"DrDATA %KDATA %K0DATA %K|DATA %KȕDCHTDSamDySN MDye#DyeNXDyeN]DyeNbDyeNgDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT FWO_GATCGTyp!!,HCFGCEHCFGHCFGHCFG==InScInVtLANE:LAST~~4TLIMS!!uLNTD2LsrPaMCHNMODF –MODL3730NAVGNLNE`NOISOfSc $P1AMT%ZP1RLT"P1WDT(P2AMT/VP2BA2P2RLT,PBAS`PBASPCON \PCONPDMFPDMFPLOCT^PLOCT PSZE`PTYPiPXLBRGNm;RMXV1RMdN %RMdV1RMdX.RPrN RPrV1RUND RUND RUND RUND RUNT 'RUNT ;#RUNT 3RUNT 9(Rate  RunN""S/N%{SCAN3SMED SMLtSMPLSPACAxjSPACSPACAxjSVER4.0SVER 5SVER,,Satd $^Scal@Scan3TUBED8Tmpr<UserphARphCHphDYbigphQLcphTRphTR_This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŸAPrV1APrX}}¨ARTNASPFASPtASPtB1PtB1PtBCTS5BufT"DqCTIDCCTNMICTOwaCTTL lCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"D.DATA"DrDATA %KDATA %K0DATA %K|DATA %KȕDCHTDSamDySN MDye#DyeNXDyeN]DyeNbDyeNgDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT FTabD8FVocD'FWO_GATCFeat//CGTyp!!,HCFGCEHCFGHCFGHCFG==InScInVtLANE:LAST~~4TLIMS!!uLNTD2LsrPaMCHNMODF –MODL3730NAVGNLNE`NOISOfSc $P1AMT%ZP1RLT"P1WDT(P2AMT/VP2BA2P2RLT,PBAS`PBASPCON \PCONPDMFPDMFPLOCT^PLOCT PSZE`PTYPiPXLBRGNm;RMXV1RMdN %RMdV1RMdX.RPrN RPrV1RUND RUND RUND RUND RUNT 'RUNT ;#RUNT 3RUNT 9(Rate  RunN""S/N%{SCAN3SMED SMLtSMPLSPACAxjSPACSPACAxjSVER4.0SVER 5SVER,,Satd $^Scal@Scan3TUBED8Tmpr<UserphARphCHphDYbigphQLcphTRphTR^pF?jA]RI<672_4CF_E[ .*ilw17*$xeK>tW\FU"7?-3U'.1P|qW>C^;=0hSvoIxT6eT*a@*_0t}{JTMF4F>hcDW4KuYGH[{OF%6+,/|8iHAj}W2yv!a!<}B]rQHfKb9hC?{r\*xu3{CW?vR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;`8hC0rOHNJw9b6mtTUk1T:29GfnGO5bLhbB/t|4unL,rg#zv{?oH[{E,8#V>Ax^c~=$~!IZHpq'_C:+0Oj!Q4KK*flK|+@O5L |J~RW)&'="%hp]`>\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|Yya,mgrh~nRnX@EV$2kE}:>&ZgL7:tl|y\@fK0\O-YG7Q$$OWFI2c+-@[3>>B%P^gWT^Fu*t&:_Gr9#(+gbk1^/.S3xCmqy=Y`gsB0PPOw[h@tC]Q#V>Ax`X~lTmt.qK|-o3o*oUnY!9s!&&v_QjXaM~3>>//x3L#Id4|Ca$"T'MT,Tc%ToN668d#w>6?xHf.QR9JK[YlyjSu30&reA <3p`g9f$bnAfV/B|m:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=H)RiA9GtXWJoe`rlx%b@cTY_eM6^sO \}!&R2{bP+*,2\]@VrauT&Mz*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;SAEPt3AEPt3APFN APXV2APrN ŸAPrV1APrX}}¨ARTNASPFASPtASPtAUDTjjT B1PtB1PtBCTS5BufT"DqCTIDCCTNMICTOwaCTTL lCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"D.DATA"DrDATA %KDATA %K0DATA %K|DATA %KȕDCHTDSamDySN MDye#DyeNXDyeN]DyeNbDyeNgDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT FTabD8FVocD'FWO_GATCFeat//CGTyp!!,HCFGCEHCFGHCFGHCFG==InScInVtLANE:LAST~~4TLIMS!!uLNTD2LsrPaMCHNMODF –MODL3730NAVGNLNE`NOISOfSc $P1AMT%ZP1RLT"P1WDT(P2AMT/VP2BA2P2RLT,PBAS`PBASPCON \PCONPDMFPDMFPLOCT^PLOCT PSZE`PTYPiPXLBRGNm;RMXV1RMdN %RMdV1RMdX.RPrN RPrV1RUND RUND RUND RUND RUNT 'RUNT ;#RUNT 3RUNT 9(Rate  RunN""S/N%{SCAN3SMED SMLtSMPLSPACAxjSPACSPACAxjSVER4.0SVER 5SVER,,Satd $^Scal@Scan3TUBED8Tmpr<UserphARphCHphDYbigphQLcphTRphTR