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GGG@@@ZZZooy     $''...555<<<BBAHHHNNNUTTZYZ``_ffejjjpppuuuzzz~~~~~~~~~~~~~~~~~~}}}}}}}}}|}}|||||||||||||{{||||||{{{{{{{{{{{{{{{zz{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{z{{{{{{z{{{{{{{{{{z{{{{{{{{{{{z{{{{{{z{{{{{{{{{{{{{{{{{{{z{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{|{{{{{{{{{|{{{{{{{{|||{{{{||||||{{{{{{{{|{{|{{{{{{{{{{{{{{{{|{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{{|{{|{{{{{|{{{|{||{{{{{|{{{{|{|||{|{{{|{|{{|{{||{||{|{{{|||{|{{{|||{{{|{||{|||||||||||||{|||||||||||||||||||||||||||||||||||||||||||||||||||||}}|||||||}}|||}}}}|}}}}|||||}}}|||}}}}}}}}}}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~}~~}~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ca616c72123b4cc98ba37cd5dd9475d0Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-30_19-29_00993130Seq1281212812_Steier_K1319_trnHjmadera96-WellK1319_C12_trnHKB_3730_POP7_BDTv3.mobSeqPop7z;=?DJRY^adipx ,;IWet+5>GOVZ[[YVQKA5%ydO:$}zxxwwwx{~{skd]XUTUUTSRQQRSUVUSQOMMMOQSTTUVWZ]agnw/^P Atg/af;?sQf)5sO eBEuU:$ !+5?IS^it}sjbXN@0 }{xtojc\TLE>83/,)'$" !#%&'())*+,,---,,,-06>HS^gnsuvvtqld[PG?;9:=@CFILOQRQOKGC@>=>@BFJMQTWYZZYWTPMJIHHILQYesl[LA82/--,,*'%"    ..026;?BBA@??@BDFGGFCA?>>>>=<:9752/,)%"        '/6<AFIJJIEA<62-*(&%'*0:ESbq !"  (6ES`lstodTA. !aRY6hY$E@l;ysmheb_\WSOLIHHJNU^iuwoifdefilprtsqmg`XQJEA><:8631.+(%#!!"%)-159=AEILNOOOPT[erG[v?hplmprqme[QG>5.' #(-2552-%          "$%')+-.//.--,+*)'%"       (?Yw 5]hI'o^RKGEDBA@?@BDGIJJJJJJJKJIHGFGHKOSW[_choxyof]VOIEB@??ACHOXajrx{}~~~|yupjd^YTNIC?<<>CJR[dkqssoh_TI?6/*'%$#"#$%&'((('(*-3:CLU\ab_XOD9.%                  !'-13568;@FMSY_dkqx            !#!   qe[UQNMKIFCBAABA@<8410/000/.,+++-/123322247<CJR[er -MlhM/umheeegilqx9\5KazN79I K  : J R Z e v } < U!IrVn wfUF8+ xohda``aa```acgkqx~}wrmieb^ZVRNJGFHLS\fpy}vmd[TMHEBABDIOV^dikjgaZRLHGHLS^m,<B>2{qh_VNE=60,*****)'&%%$$$$######"        !#%'(*+,-.////.,+)(&$#!  /_s@UA AOYAWKB.bcpKB_3730_POP7_BDTv3.mobtermTAGGTCGCGCGAGCGAATGACATACATGCGTACGAACTTTTCTTTTTGTTTCGTTTTTCTAAATTCAATTTTTTTTAAGGTCCGAAAAACAATTATCCCTTTTGGTTTCCGATACACATTGTTTTTTTAATCTACATAAGGCTACAACAACTTTACATCTAAACCCCGGAAAAAAACTCTGGAGACCTATCCCTTCCCTTCCCAGCACACGAAATTGGTGAGGGCGCACAAATTTCTCGGTCTTCTAACCCTACCAACCTCCCCCTTACCCATTTCCGGTTCGCATTTCACGAATAGGTCGCGCGAGCGAATGACATACATGCGTACGAACTTTTCTTTTTGTTTCGTTTTTCTAAATTCAATTTTTTTTAAGGTCCGAAAAACAATTATCCCTTTTGGTTTCCGATACACATTGTTTTTTTAATCTACATAAGGCTACAACAACTTTACATCTAAACCCCGGAAAAAAACTCTGGAGACCTATCCCTTCCCTTCCCAGCACACGAAATTGGTGAGGGCGCACAAATTTCTCGGTCTTCTAACCCTACCAACCTCCCCCTTACCCATTTCCGGTTCGCATTTCACGAA38LYgy(4BKYh &?GUcp"8Oft!,8EN^hu'4IValt)3BLYbn/8DR[kq} ",=M]ly    & 4 @ L Y h o ~    + 7 F O [ h w    ( 3 < J X c n {  " . 7 B O [ e u   % 0 : E S ] i w -=FN^is38LYgy(4BKYh &?GUcp"8Oft!,8EN^hu'4IValt)3BLYbn/8DR[kq} ",=M]ly    & 4 @ L Y h o ~    + 7 F O [ h w    ( 3 < J X c n {  " . 7 B O [ e u   % 0 : E S ] i w -=FN^is                                            !# "           "                                                !# "           "     @_)Z5^H^bYPZ7!DXa)4/G (+)GO 4QZC9a% _Y`  ;S 21a<B>+b=Q!U-H _-T 54JCLaUc)>!`0V,3bA%N?O"D8[K_RRJN4b!3<d6X*:b2M 1:5/**`:*:*:*9\7";231:c13F13J13T 3b1Y: J13 Z@#1]Q<]8],8PTN]OAM,6N"!28+0=bL !^MK,88bZ+U;a$8\ c9c, 1O2aP;JEe/  NA,J P` xSJ`r, vzL{|Ra(5"cIfblX |!vl* %bY G'!sgoos}[M#Of}cY z,E(n!!6 %OulmaVW0 Wes6~lEL lKML5nEyZfj@+Z3&Q?CRO !aZ|)On{d%[  GY 3+&iO\>}fz< +J#OW/,uR\2<h"oXT! !/-|j (-  2? s #$ *6Fp_qQ_Rfp1?({N4mA*07zzQ!%c b (>,O[l u*|*@aa 99/4t,6vl6Vo|j~=t(,LG48\`fR ,=[7,D<,aB;dK=@5UC|=_3T ~+<O$!4s9 F, tn.d9Ml3*ogaI;w-ne$W Azt\t{D]nI*~ddFn8GKg6r @%89\4zx31;!>UK,bx^EZ~F#EX0Ys +3 +dB_- @iK7+DlQM>6;aE|O<s fpK}k&/w QAAGCCCGCGCGCGCGAGCCCCACCTTCGTGTAGAAGGNCACATAAAAAAGCCTCCACAAAGTCTACGCTCCCCCCAAGGTTGATTCTTGCTGGACTGTATACAGATCCATACGAGATTGGCTCCCAAAAGTATACATATTCTTAGAGTGGGGGCCGATGTCGGCAATTCTCCCTTGTACGATATCCTTGTGGATCAAGTGCGATGCACAGAACTCAGAACTGTTCGCGGGCTCAACGAAACCAAAAATTTTTCTTTGTATAAAGTTCCTGTTTACCGTAAAATAATCACACTATCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-30 21:01:00 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt ASPt B1PtB1PtBCTS$ BufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $IDATA $I.DATA $IxDATA $I~DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~"LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS |P1AM&L+P1RL&LP1WD&LwP2AM&LP2BA&&![P2RL&LPBAS&& PBAS&& PCON&&PCON&&PDMF|PDMF PLOC&LGPLOC&LPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 'RUNT ;#RUNT 3RUNT 9(Rate RunN""S/N% tSCAN3SMED SMLtSMPLmSPACA}SPAC SPACA}SVER4.0SVER #SVER,,nScal@Scan3TUBEC12Tmpr<UserphARphCH phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt ASPt B1PtB1PtBCTS$ BufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $IDATA $I.DATA $IxDATA $I~DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab2dFVoc2SFWO_GATCFeat..2%GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~"LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS |P1AM&L+P1RL&LP1WD&LwP2AM&LP2BA&&![P2RL&LPBAS&& PBAS&& PCON&&PCON&&PDMF|PDMF PLOC&LGPLOC&LPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 'RUNT ;#RUNT 3RUNT 9(Rate RunN""S/N% tSCAN3SMED SMLtSMPLmSPACA}SPAC SPACA}SVER4.0SVER #SVER,,nScal@Scan3TUBEC12Tmpr<UserphARphCH phDYbigphQLcphTRphTR@~A67OQ1{zK90plkm$48V^p$&:?wMB>-j%;U*E^h9i_Qw|k8 <.(P-S"EnKl/:5t9z"DEoh9ZzZW-W 7W}$wFTN]%jEX@u]YMAmB] ]ddc}PMfOl>o UekB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RUR6? sdWUW>|})15]Ahk95HK&]e"-%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tr%,yPyN+8ds16;evr./b?Ay0]:jjMSDDl[zLTHArK(\oi$S1[!h3}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03Sx*dW0Vq&!Z=c':Z^STt4)TK%FyPD[6A|zvn[Y<,h`%mI{Mb=K{_"#kI>b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~6>udrV0HJA@vd*W]U_I[Ddg/