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$##+**111888>>>DDGJJPQPVVV\\\bbbggglllrrqvvv|{{~~~~~~~~~~~~~~~}}}}}}}}}~}~}~}~}~~}}}}~~~}}~~}}}}~~~}}}~}~~}~~}~~~~~~~~~~~~~~~~~~~~}~}}~~~~}~~~~~}}~~}~}}}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 06ba66a9dcd54a939a881179fabc01ccSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-23_15-53_0062 3130Seq 12769 12769_Steier_K1320_trnH jmadera 96-Well
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o x Z 6 = U r 5 ] : o 0 } * V r V + 3 1 G + R 8 P a < [ b \ * / C ; \ X 5 V W U [ T Y @ h X j . Y 2 h J " d M N @ 2 4 ? X X p G r \ " g c e 4R GAGACCGCGCGAGGGCCAAGCCCGTCGAACCTGCGAATATAAATTTAAATAGTAAATAACTATGTGCACAATAAACACCACCCATCAAAGGATGTCTAGAGTATACCGCCGGTTTCATGGTTCCGTCAGACAATTGGTATTGGCTCCAGCATTATCTACTAGCTCGACACTTTTTGAGTTCACAATAGCGCTTCAAGACCAACTCTGAGGCCTGTACTGGGTACTGGCCTATGTAAAATATTCCCAAATTTAAATAGGGGCCAGAAGCTGCCCATAGAGTCAGGTCATGAGAATGGTGACCATGCAGGCCCAGGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-23 17:24:22 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ; BufT & L I CTID CTNM ! CTOw 9 CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA ' Od DATA
' Od 4' DATA ' Od DATA ' Od DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 2 LAST ~ ~ : LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS "[ P1AM < x . P1RL < x ,n P1WD < x 1^ P2AM < x 6N P2BA < < 8 P2RL < x 3 PBAS < < # PBAS < < " PCON < < +2 PCON < < ) PDMF PDMF "r PLOC < x '~ PLOC < x % PSZE ` PTYP A PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 5 RUNT # RUNT / RUNT Rate RunN " " S/N% "S SCAN 3 SMED
SMLt SMPL SPAC M SPAC "k SPAC M SVER 4.0 SVER ; SVER , , Scal @ Scan 3 TUBE H8 Tmpr < User phAR phCH " phDY big phQL c phTR phTR '6 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ; BufT & L I CTID CTNM ! CTOw 9 CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA ' Od DATA
' Od 4' DATA ' Od DATA ' Od DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab I FVoc I FWO_ GATC Feat / / I GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 2 LAST ~ ~ : LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS "[ P1AM < x . P1RL < x ,n P1WD < x 1^ P2AM < x 6N P2BA < < 8 P2RL < x 3 PBAS < < # PBAS < < " PCON < < +2 PCON < < ) PDMF PDMF "r PLOC < x '~ PLOC < x % PSZE ` PTYP A PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 5 RUNT # RUNT / RUNT Rate RunN " " S/N% "S SCAN 3 SMED
SMLt SMPL SPAC M SPAC "k SPAC M SVER 4.0 SVER ; SVER , , Scal @ Scan 3 TUBE H8 Tmpr < User phAR phCH " phDY big phQL c phTR phTR PF{ytl+hcDW4KuYGH[{O!QECN&0@]/0dwm\e+<}P?sw!iOup_3:E-v^gMs7M9{SHM6Fnf%)qk&YgDS7}_'AR;L6,8efj+l-uk#%C`]@zMxLrLei;E]yKoS]8qUvn}4|Mdna'"`ko`^ kg"#]eV&U)>`V2]<|':eqM?k3P}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47.'s1Hhl@Ft/9p<13#~FTl$A0V.[(7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MKVRJxG57'9\47.<<_