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"     #            " &                       '!          #       $(#'  !      !        *%+,/#0)!+!.4                                  "                        89:;<=>?@ABCUVWXYZ[\]^_`abcde89:;<=>?@ABCUVWXYZ[\]^_`abcdeY,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR*               FFF???YYYmmm~~~   $##+**111888>>>DDGJJPQPVVV\\\bbbggglllrrqvvv|{{~~~~~~~~~~~~~~~}}}}}}}}}~}~}~}~}~~}}}}~~~}}~~}}}}~~~}}}~}~~}~~}~~~~~~~~~~~~~~~~~~~~}~}}~~~~}~~~~~}}~~}~}}}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: cf78b4f384774cd9b569311e1e4f1e18Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-23_15-53_00623130Seq1276912769_Steier_K1320_trnHjmadera96-Well K1320_G2_trnHKB_3730_POP7_BDTv3.mobSeqPop7zj\I19^} As!2:DTnEN= 0 p p\E L$z]!N#=% &(*,Z.d03`6^9<@.CQFNI0LNQTWZJ\]]^~^j]\:ZZXUROLJGADB ?m<: 75d322]2X23N4V579;=>@@ACDxEG5HHHxGGF DCBBlBlBCEFHqJM\PTuX]c.i+ovz}Q~xrOkd]BVO^I:C>950,Q(&$""!M r88h " !D!!"P"#$&J'(*n-4@Pcz|+vqnqlLk?jjjjjjjjihfdb$_]`[ZZYZ YYYDXXXYY[\ \\\m[rZ8YXXY~Z\^_<`!`ajab}bbbaU```4abbdefgjfe@by^YTNIC~>84/_*&#JAB$ : - o G 8 ,  5 q @gzqJ e  "V9Af`aW9 |7,PU< UK{ ZvI`JK[m{,bBNm,g[_m{|mXB. #**&$*;Pbh]F) #(&,H]e[E(#4@A8)  'A_x}eB! )Z&UkhR3hWS^srP. 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"&  )/*                                       %)& "!                      !                %#                   #('!        !   %%  %&"  "        !%           %%         "'(%                                           %-.&   @A A5&@\)KB.bcpKB_3730_POP7_BDTv3.mobtermATTAGTCACACATTAAGGATATGTACTCTCTCGGCCCTCTCTATCTATTATCAAGTCTAGTATATGATAAGGTAGTTTAGAAAAGATTTATAGATTACTAAAACATATTACTTTCTTTTTAAAAAAATAATATAGGAGCAATAACGCCCTCTTTGAAAAACAAGAGGGATGCTATTGCTCCATTTTTATTTCTGAAGGCCGATACATTGCAATCGAACCAAGATGGGTGGGGTGAGATGGAAGGGAAAAAAGCCATTCCAATTAGTCACACATTAAGGATATGTACTCTCTCGGCCCTCTCTATCTATTATCAAGTCTAGTATATGATAAGGTAGTTTAGAAAAGATTTATAGATTACTAAAACATATTACTTTCTTTTTAAAAAAATAATATAGGAGCAATAACGCCCTCTTTGAAAAACAAGAGGGATGCTATTGCTCCATTTTTATTTCTGAAGGCCGATACATTGCAATCGAACCAAGATGGGTGGGGTGAGATGGAAGGGAAAAAAGCCATTCCA&=\n />K[jw$2<EXfs*8BPYdp#5BM]fq *7BM[ir|"/:FPYeks )5ALW_oy)5@IVcoy   , 8 E P ^ h v ~    * 6 ? L W ` o w    ) 5 C R a o }    - 8 B S _ l w  &=\n />K[jw$2<EXfs*8BPYdp#5BM]fq *7BM[ir|"/:FPYeks )5ALW_oy)5@IVcoy   , 8 E P ^ h v ~    * 6 ? L W ` o w    ) 5 C R a o }    - 8 B S _ l w                  '           "  "        (                           '           "  "        (            cNV* N$22(cMRS/A AM,^F4IT @^;!]VRVd UW]T[I(`S]7-[5U\c.S@4 YOZ)BEYa;U9 TM&$2 S9@K4(6 \Z! ""W*3T1M%AYK &9@X$,G!a/_'SEQD^PA''8U=(_=(1-'=1 S7\\92C"2\G:KLM\&\F2D\1'V!IC=2ZPR\E\%2@.2\J  D7 g/%ED_P p# V33mCniW o$NnJ75lfN ( V1U6U# YZJ1vZx D  Q F G  Yiq8k&` ~^W`PFB\g=`xd]\ZN"iyocA/-1 O,W >fBG; M $U u*^A|H"V\{RkR!#\ Je0 H" _?MCBHgWGjhr@,['X@+ SM'0+S$: z_q9!"s !\%)l >^U6JJ5,b$!B+mh\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\:,p#|ppCSp (JoUj45}IdpCwK_ K0+Hb/8r')mK(1_,[<v\d.A<k5/f]Y0b\| D=l`3vrmYyM-J!5-B d  d.M.McV}! Qe+l|5>28|k` Vo.MT3:]aA=:#%0#3 cA Hh{ R+MC^@b29CH[s`OW`g8)?"*J0wq O,n\EZ &Pa4[7 jNvM:`^MhmlX/<V"1WsS$ yXCAACCGGGGGGCCGGGAACCCACGCAATAAGGCCGATCTAAATAAACGAGGTTTAAAACTATACGTTACGATGCCCCCTTTTTGATCCCTCGATGCGTACTTCATGGAAGGCCCTCCCCATTTTGCCGGGGTAGTTGAATTCGGAATGTATCAATGGTTGGGGTCTTAGATGCTCCCACTACAACAATGCCTCATTCATATCCCGCAGCGGTGTAGGAAGGAGGCTAGCCATGATCTCTTTGTCCGCTGGCAAACCCATGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-23 17:23:46 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ÈARTNASPFASPtASPtB1PtB1PtBCTS+BufT&LQCTID#CTNM)CTOwACTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&LfDATA&LDATA&LDATA&LJDATA $*HTDATA $*HT.DATA $*HTvsDATA $*HTDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS#OfSc!P1AM~P1RLvP1WDP2AMP2BAP2RLPBASZPBASVPCONrPCON nPDMFPDMF:PLOC fPLOC ^PSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5RUNT #RUNT /RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACMSPAC3SPACMSVER4.0SVER  SVER,,–Satd!^Scal@Scan3TUBEG2Tmpr<UserphARphCHQphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ÈARTNASPFASPtASPtB1PtB1PtBCTS+BufT&LQCTID#CTNM)CTOwACTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&LfDATA&LDATA&LDATA&LJDATA $*HTDATA $*HT.DATA $*HTvsDATA $*HTDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab*FVoc*tFWO_GATCFeat..*FGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS#OfSc!P1AM~P1RLvP1WDP2AMP2BAP2RLPBASZPBASVPCONrPCON nPDMFPDMF:PLOC fPLOC ^PSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5RUNT #RUNT /RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACMSPAC3SPACMSVER4.0SVER  SVER,,–Satd!^Scal@Scan3TUBEG2Tmpr<UserphARphCHQphDYbigphQLcphTRphTRRz0L/kbE!0z^n3HVQQEO2astcZ~~VGC2lB)lW+Uf066}ZC__cIBM}.z zFef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`P|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTveurDmCq?`DQ`+}{Uplql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W@Vk1$#=4ZbZ4yM=VrTH&zqrua*b iq*#7rO/adu{>(Jt6qZ><}P?[NB@vulc*{w@p;#(":?S`,j.uFTcM#yKA#2rBA++muIl4NU[|18$ Gw]x*,4"B (_e:RJ4ir$G(~}d M]f)C^DZ=<7:cmj^k-T0M5[S*.JpxcSX`"lef5U_9}|Ate1}1"/bW>y&bC]LVn!<|'k0|3.B+0Oj!Q4X+*flaO8JB6E$} {r*i`V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3*-CdM]4e):EGa9?QLwUF3TP.&"D|;f[5kr+E{@2K/^1bf+kT#*\"MiD$bWz%I!|spN$t.DkrD5pY(d+{o.jaxJK[Ylid?O>JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUG